O75110 (ATP9A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable phospholipid-transporting ATPase IIA
EC=3.6.3.1

Alternative name(s):
ATPase class II type 9A

Gene names

Name:	ATP9A
Synonyms:	ATPIIA, KIAA0611

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1047 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + H2O + phospholipid(In) = ADP + phosphate + phospholipid(Out).
Subcellular location	Membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily. [View classification]
Sequence caution	The sequence BAD18775.1 differs from that shown. Reason: Erroneous initiation. The sequence CAI18889.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI19203.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI22924.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cation transport Inferred from electronic annotation. Source: InterPro phospholipid translocation Non-traceable author statement. Source: UniProtKB
Cellular component	early endosome Inferred from direct assay. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW perinuclear region of cytoplasm Inferred from direct assay. Source: UniProtKB recycling endosome Inferred from direct assay. Source: UniProtKB trans-Golgi network Inferred from direct assay. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: InterPro phospholipid-translocating ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: O75110-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: O75110-2)

The sequence of this isoform differs from the canonical sequence as follows:
     149-269: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1047	1047	Probable phospholipid-transporting ATPase IIA		PRO_0000046375
Regions
	Topological domain	1 – 69	69	Cytoplasmic Potential
	Transmembrane	70 – 91	22	Helical; Potential
	Topological domain	92 – 96	5	Extracellular Potential
	Transmembrane	97 – 119	23	Helical; Potential
	Topological domain	120 – 303	184	Cytoplasmic Potential
	Transmembrane	304 – 325	22	Helical; Potential
	Topological domain	326 – 332	7	Extracellular Potential
	Transmembrane	333 – 354	22	Helical; Potential
	Topological domain	355 – 841	487	Cytoplasmic Potential
	Transmembrane	842 – 862	21	Helical; Potential
	Topological domain	863 – 874	12	Extracellular Potential
	Transmembrane	875 – 893	19	Helical; Potential
	Topological domain	894 – 923	30	Cytoplasmic Potential
	Transmembrane	924 – 942	19	Helical; Potential
	Topological domain	943 – 949	7	Extracellular Potential
	Transmembrane	950 – 972	23	Helical; Potential
	Topological domain	973 – 978	6	Cytoplasmic Potential
	Transmembrane	979 – 999	21	Helical; Potential
	Topological domain	1000 – 1006	7	Extracellular Potential
	Transmembrane	1007 – 1030	24	Helical; Potential
	Topological domain	1031 – 1047	17	Cytoplasmic Potential
Sites
	Active site	391	1	4-aspartylphosphate intermediate By similarity
	Metal binding	785	1	Magnesium By similarity
	Metal binding	789	1	Magnesium By similarity
Natural variations
	Alternative sequence	149 – 269	121	Missing in isoform Short.		VSP_000432
Experimental info
	Sequence conflict	186	1	F → S in BAD18775. Ref.4
	Sequence conflict	415	1	L → P in BAD18775. Ref.4
	Sequence conflict	957	1	S → P in BAD18775. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified January 24, 2001. Version 3. Checksum: 80C307CF5A396755	FASTA	1,047	118,583
	10 20 30 40 50 60 MTDNIPLQPV RQKKRMDSRP RAGCCEWLRC CGGGEARPRT VWLGHPEKRD QRYPRNVINN 70 80 90 100 110 120 QKYNFFTFLP GVLFNQFKYF FNLYFLLLAC SQFVPEMRLG ALYTYWVPLG FVLAVTVIRE 130 140 150 160 170 180 AVEEIRCYVR DKEVNSQVYS RLTARGTVKV KSSNIQVGDL IIVEKNQRVP ADMIFLRTSE 190 200 210 220 230 240 KNGSCFLRTD QLDGETDWKL RLPVACTQRL PTAADLLQIR SYVYAEEPNI DIHNFVGTFT 250 260 270 280 290 300 REDSDPPISE SLSIENTLWA GTVVASGTVV GVVLYTGREL RSVMNTSNPR SKIGLFDLEV 310 320 330 340 350 360 NCLTKILFGA LVVVSLVMVA LQHFAGRWYL QIIRFLLLFS NIIPISLRVN LDMGKIVYSW 370 380 390 400 410 420 VIRRDSKIPG TVVRSSTIPE QLGRISYLLT DKTGTLTQNE MIFKRLHLGT VAYGLDSMDE 430 440 450 460 470 480 VQSHIFSIYT QQSQDPPAQK GPTLTTKVRR TMSSRVHEAV KAIALCHNVT PVYESNGVTD 490 500 510 520 530 540 QAEAEKQYED SCRVYQASSP DEVALVQWTE SVGLTLVGRD QSSMQLRTPG DQILNFTILQ 550 560 570 580 590 600 IFPFTYESKR MGIIVRDEST GEITFYMKGA DVVMAGIVQY NDWLEEECGN MAREGLRVLV 610 620 630 640 650 660 VAKKSLAEEQ YQDFEARYVQ AKLSVHDRSL KVATVIESLE MEMELLCLTG VEDQLQADVR 670 680 690 700 710 720 PTLETLRNAG IKVWMLTGDK LETATCTAKN AHLVTRNQDI HVFRLVTNRG EAHLELNAFR 730 740 750 760 770 780 RKHDCALVIS GDSLEVCLKY YEYEFMELAC QCPAVVCCRC APTQKAQIVR LLQERTGKLT 790 800 810 820 830 840 CAVGDGGNDV SMIQESDCGV GVEGKEGKQA SLAADFSITQ FKHLGRLLMV HGRNSYKRSA 850 860 870 880 890 900 ALSQFVIHRS LCISTMQAVF SSVFYFASVP LYQGFLIIGY STIYTMFPVF SLVLDKDVKS 910 920 930 940 950 960 EVAMLYPELY KDLLKGRPLS YKTFLIWVLI SIYQGSTIMY GALLLFESEF VHIVAISFTS 970 980 990 1000 1010 1020 LILTELLMVA LTIQTWHWLM TVAELLSLAC YIASLVFLHE FIDVYFIATL SFLWKVSVIT 1030 1040 LVSCLPLYVL KYLRRRFSPP SYSKLTS
	Isoform Short [UniParc]. Checksum: F2F66A97B9000AA4	FASTA	926	105,133
	10 20 30 40 50 60 MTDNIPLQPV RQKKRMDSRP RAGCCEWLRC CGGGEARPRT VWLGHPEKRD QRYPRNVINN 70 80 90 100 110 120 QKYNFFTFLP GVLFNQFKYF FNLYFLLLAC SQFVPEMRLG ALYTYWVPLG FVLAVTVIRE 130 140 150 160 170 180 AVEEIRCYVR DKEVNSQVYS RLTARGTVVG VVLYTGRELR SVMNTSNPRS KIGLFDLEVN 190 200 210 220 230 240 CLTKILFGAL VVVSLVMVAL QHFAGRWYLQ IIRFLLLFSN IIPISLRVNL DMGKIVYSWV 250 260 270 280 290 300 IRRDSKIPGT VVRSSTIPEQ LGRISYLLTD KTGTLTQNEM IFKRLHLGTV AYGLDSMDEV 310 320 330 340 350 360 QSHIFSIYTQ QSQDPPAQKG PTLTTKVRRT MSSRVHEAVK AIALCHNVTP VYESNGVTDQ 370 380 390 400 410 420 AEAEKQYEDS CRVYQASSPD EVALVQWTES VGLTLVGRDQ SSMQLRTPGD QILNFTILQI 430 440 450 460 470 480 FPFTYESKRM GIIVRDESTG EITFYMKGAD VVMAGIVQYN DWLEEECGNM AREGLRVLVV 490 500 510 520 530 540 AKKSLAEEQY QDFEARYVQA KLSVHDRSLK VATVIESLEM EMELLCLTGV EDQLQADVRP 550 560 570 580 590 600 TLETLRNAGI KVWMLTGDKL ETATCTAKNA HLVTRNQDIH VFRLVTNRGE AHLELNAFRR 610 620 630 640 650 660 KHDCALVISG DSLEVCLKYY EYEFMELACQ CPAVVCCRCA PTQKAQIVRL LQERTGKLTC 670 680 690 700 710 720 AVGDGGNDVS MIQESDCGVG VEGKEGKQAS LAADFSITQF KHLGRLLMVH GRNSYKRSAA 730 740 750 760 770 780 LSQFVIHRSL CISTMQAVFS SVFYFASVPL YQGFLIIGYS TIYTMFPVFS LVLDKDVKSE 790 800 810 820 830 840 VAMLYPELYK DLLKGRPLSY KTFLIWVLIS IYQGSTIMYG ALLLFESEFV HIVAISFTSL 850 860 870 880 890 900 ILTELLMVAL TIQTWHWLMT VAELLSLACY IASLVFLHEF IDVYFIATLS FLWKVSVITL 910 920 VSCLPLYVLK YLRRRFSPPS YSKLTS

References

[1]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed: 11780052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Ishikawa K., Nagase T., Suyama M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:169-176(1998) [PubMed: 9734811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 15-1047 (ISOFORM SHORT). Tissue: Brain.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 91-1047 (ISOFORM LONG). Tissue: Hepatoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL353799, AL138807, AL035684 Genomic DNA. Translation: CAI18889.1. Sequence problems. AL353799, AL035684, AL138807 Genomic DNA. Translation: CAI18890.1. AL035684, AL138807, AL353799 Genomic DNA. Translation: CAI19202.1. AL035684, AL138807, AL353799 Genomic DNA. Translation: CAI19203.1. Sequence problems. AL138807, AL035684, AL353799 Genomic DNA. Translation: CAI22924.1. Sequence problems. AL138807, AL035684, AL353799 Genomic DNA. Translation: CAI22925.1. CH471077 Genomic DNA. Translation: EAW75597.1. CH471077 Genomic DNA. Translation: EAW75598.1. CH471077 Genomic DNA. Translation: EAW75599.1. CH471077 Genomic DNA. Translation: EAW75600.1. AB014511 mRNA. Translation: BAA31586.1. AK172802 mRNA. Translation: BAD18775.1. Different initiation.
IPI	IPI00024368. IPI00220239.
RefSeq	NP_006036.1. NM_006045.1.
UniGene	Hs.726016.
3D structure databases
ProteinModelPortal	O75110.
SMR	O75110. Positions 757-820.
ModBase	Search...
Protein-protein interaction databases
IntAct	O75110. 1 interaction.
STRING	O75110.
Proteomic databases
PRIDE	O75110.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000338821; ENSP00000342481; ENSG00000054793.
GeneID	10079.
KEGG	hsa:10079.
UCSC	uc002xwg.1. human. uc010gih.1. human.
Organism-specific databases
CTD	10079.
GeneCards	GC20M050213.
HGNC	HGNC:13540. ATP9A.
HPA	CAB020699. HPA035630.
MIM	609126. gene.
neXtProt	NX_O75110.
PharmGKB	PA25171.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG15792.
HOVERGEN	HBG050617.
InParanoid	O75110.
OMA	SIIMYGA.
OrthoDB	EOG4T1HKQ.
PhylomeDB	O75110.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules.
Gene expression databases
ArrayExpress	O75110.
Bgee	O75110.
CleanEx	HS_ATP9A.
Genevestigator	O75110.
GermOnline	ENSG00000054793. Homo sapiens.
Family and domain databases
InterPro	IPR023306. ATPase_cation_domN. IPR008250. ATPase_P-typ_ATPase-assoc-dom. IPR023300. ATPase_P-typ_cyto_domA. IPR023299. ATPase_P-typ_cyto_domN. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR006539. ATPase_P-typ_Plipid-transl. IPR023214. HAD-like_dom. [Graphical view]
Gene3D	G3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 2 hits. G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 1 hit. G3DSA:3.40.50.1000. HAD-like_dom. 2 hits.
KO	K01530.
Pfam	PF00122. E1-E2_ATPase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE.
SUPFAM	SSF81660. ATPase_cation_domN. 1 hit. SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01652. ATPase-Plipid. 1 hit. TIGR01494. ATPase_P-type. 2 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	38107.
SOURCE	Search...

Entry information

Entry name

ATP9A_HUMAN

Accession

Primary (citable) accession number: O75110
Secondary accession number(s): E1P5Y3 , E1P5Y4, Q5TFW5, Q5TFW6, Q5TFW9, Q6ZMF3, Q9NQK6, Q9NQK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 24, 2001
Last modified:	January 25, 2012
This is version 107 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.