O75083 (WDR1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
WD repeat-containing protein 1

Alternative name(s):
Actin-interacting protein 1
Short name=AIP1
NORI-1

Gene names

Name:

WDR1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	606 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Induces disassembly of actin filaments in conjunction with ADF/cofilin family proteins By similarity.
Subcellular location	Cytoplasm › cytoskeleton By similarity.
Sequence similarities	Belongs to the WD repeat AIP1 family. Contains 11 WD repeats.
Sequence caution	The sequence AAD05045.1 differs from that shown. Reason: Aberrant splicing.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat WD repeat
Ligand	Actin-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	platelet activation Traceable author statement. Source: Reactome platelet degranulation Traceable author statement. Source: Reactome sensory perception of sound Traceable author statement. Source: ProtInc
Cellular component	cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Traceable author statement. Source: Reactome extracellular region Traceable author statement. Source: Reactome
Molecular function	actin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O75083-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O75083-3)

The sequence of this isoform differs from the canonical sequence as follows:
     47-186: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.6
	Chain	2 – 606	605	WD repeat-containing protein 1		PRO_0000051341
Regions
	Repeat	56 – 95	40	WD 1
	Repeat	100 – 143	44	WD 2
	Repeat	144 – 184	41	WD 3
	Repeat	187 – 226	40	WD 4
	Repeat	232 – 271	40	WD 5
	Repeat	318 – 359	42	WD 6
	Repeat	363 – 401	39	WD 7
	Repeat	443 – 482	40	WD 8
	Repeat	487 – 526	40	WD 9
	Repeat	530 – 569	40	WD 10
	Repeat	574 – 605	32	WD 11
Amino acid modifications
	Modified residue	28	1	N6-acetyllysine Ref.11
	Modified residue	81	1	N6-acetyllysine Ref.11
	Modified residue	95	1	N6-acetyllysine Ref.11
	Modified residue	115	1	N6-acetyllysine Ref.11
	Modified residue	238	1	Phosphotyrosine Ref.9 Ref.10
	Modified residue	480	1	N6-acetyllysine Ref.11
Natural variations
	Alternative sequence	47 – 186	140	Missing in isoform 2.		VSP_012926
	Natural variant	185	1	I → V. Ref.5 Corresponds to variant rs13441 [ dbSNP | Ensembl ].		VAR_013445

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 8C08C31D5CD097F1	FASTA	606	66,194
	10 20 30 40 50 60 MPYEIKKVFA SLPQVERGVS KIIGGDPKGN NFLYTNGKCV ILRNIDNPAL ADIYTEHAHQ 70 80 90 100 110 120 VVVAKYAPSG FYIASGDVSG KLRIWDTTQK EHLLKYEYQP FAGKIKDIAW TEDSKRIAVV 130 140 150 160 170 180 GEGREKFGAV FLWDSGSSVG EITGHNKVIN SVDIKQSRPY RLATGSDDNC AAFFEGPPFK 190 200 210 220 230 240 FKFTIGDHSR FVNCVRFSPD GNRFATASAD GQIYIYDGKT GEKVCALGGS KAHDGGIYAI 250 260 270 280 290 300 SWSPDSTHLL SASGDKTSKI WDVSVNSVVS TFPMGSTVLD QQLGCLWQKD HLLSVSLSGY 310 320 330 340 350 360 INYLDRNNPS KPLHVIKGHS KSIQCLTVHK NGGKSYIYSG SHDGHINYWD SETGENDSFA 370 380 390 400 410 420 GKGHTNQVSR MTVDESGQLI SCSMDDTVRY TSLMLRDYSG QGVVKLDVQP KCVAVGPGGY 430 440 450 460 470 480 AVVVCIGQIV LLKDQRKCFS IDNPGYEPEV VAVHPGGDTV AIGGVDGNVR LYSILGTTLK 490 500 510 520 530 540 DEGKLLEAKG PVTDVAYSHD GAFLAVCDAS KVVTVFSVAD GYSENNVFYG HHAKIVCLAW 550 560 570 580 590 600 SPDNEHFASG GMDMMVYVWT LSDPETRVKI QDAHRLHHVS SLAWLDEHTL VTTSHDASVK EWTITY
	Isoform 2 [UniParc]. Checksum: 4CC6C543EB6F365D	FASTA	466	50,694
	10 20 30 40 50 60 MPYEIKKVFA SLPQVERGVS KIIGGDPKGN NFLYTNGKCV ILRNIDDHSR FVNCVRFSPD 70 80 90 100 110 120 GNRFATASAD GQIYIYDGKT GEKVCALGGS KAHDGGIYAI SWSPDSTHLL SASGDKTSKI 130 140 150 160 170 180 WDVSVNSVVS TFPMGSTVLD QQLGCLWQKD HLLSVSLSGY INYLDRNNPS KPLHVIKGHS 190 200 210 220 230 240 KSIQCLTVHK NGGKSYIYSG SHDGHINYWD SETGENDSFA GKGHTNQVSR MTVDESGQLI 250 260 270 280 290 300 SCSMDDTVRY TSLMLRDYSG QGVVKLDVQP KCVAVGPGGY AVVVCIGQIV LLKDQRKCFS 310 320 330 340 350 360 IDNPGYEPEV VAVHPGGDTV AIGGVDGNVR LYSILGTTLK DEGKLLEAKG PVTDVAYSHD 370 380 390 400 410 420 GAFLAVCDAS KVVTVFSVAD GYSENNVFYG HHAKIVCLAW SPDNEHFASG GMDMMVYVWT 430 440 450 460 LSDPETRVKI QDAHRLHHVS SLAWLDEHTL VTTSHDASVK EWTITY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A gene upregulated in the acoustically damaged chick basilar papilla encodes a novel WD40 repeat protein." Adler H.J., Winnicki R.S., Gong T.-W.L., Lomax M.I. Genomics 56:59-69(1999) [PubMed: 10036186] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	Abe Y., Nezu K., Ueda N. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Lymphoid tissue.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Placenta.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT VAL-185. Tissue: Colon, Eye and Mammary gland.
[6]	"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed: 12665801] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-17 (ISOFORMS 1/2). Tissue: Platelet.
[7]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 58-606 (ISOFORM 1). Tissue: Uterus.
[8]	Lubec G., Afjehi-Sadat L. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 290-306 (ISOFORMS 1/2), MASS SPECTROMETRY. Tissue: Brain and Cajal-Retzius cell.
[9]	"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed: 15592455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-238, MASS SPECTROMETRY.
[10]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-238, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[11]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-28; LYS-81; LYS-95; LYS-115 AND LYS-480, MASS SPECTROMETRY.
[12]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF020056 mRNA. Translation: AAD05044.1. AF020260 mRNA. Translation: AAD05045.1. Sequence problems. AB010427 mRNA. Translation: BAA31855.2. AK291614 mRNA. Translation: BAF84303.1. AC005674 Genomic DNA. No translation available. AC093664 Genomic DNA. No translation available. BC000201 mRNA. Translation: AAH00201.1. BC002489 mRNA. Translation: AAH02489.1. BC030541 mRNA. Translation: AAH30541.1. AL050108 mRNA. Translation: CAB43276.1. AL110208 mRNA. Translation: CAB53674.2.
IPI	IPI00746165. IPI00903292.
PIR	T13152.
RefSeq	NP_005103.2. NM_005112.4. NP_059830.1. NM_017491.3.
UniGene	Hs.128548. Hs.713658.
3D structure databases
ProteinModelPortal	O75083.
SMR	O75083. Positions 2-606.
ModBase	Search...
Protein-protein interaction databases
IntAct	O75083. 3 interactions.
STRING	O75083.
PTM databases
PhosphoSite	O75083.
2D gel databases
OGP	O75083.
REPRODUCTION-2DPAGE	IPI00746165. O75083.
UCD-2DPAGE	O75083.
Proteomic databases
PRIDE	O75083.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000382452; ENSP00000371890; ENSG00000071127.
GeneID	9948.
KEGG	hsa:9948.
UCSC	uc003gmf.1. human. uc003gmg.1. human.
Organism-specific databases
CTD	9948.
GeneCards	GC04M010075.
H-InvDB	HIX0004092.
HGNC	HGNC:12754. WDR1.
HPA	CAB020804.
MIM	604734. gene.
neXtProt	NX_O75083.
PharmGKB	PA37358.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG08918.
HOVERGEN	HBG000780.
PhylomeDB	O75083.
Enzyme and pathway databases
Reactome	REACT_604. Hemostasis.
Gene expression databases
ArrayExpress	O75083.
Bgee	O75083.
CleanEx	HS_WDR1.
Genevestigator	O75083.
GermOnline	ENSG00000071127. Homo sapiens.
Family and domain databases
InterPro	IPR020472. G-protein_beta_WD-40_rep. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 2 hits.
Pfam	PF00400. WD40. 10 hits. [Graphical view]
PRINTS	PR00320. GPROTEINBRPT.
SMART	SM00320. WD40. 11 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 2 hits.
PROSITE	PS00678. WD_REPEATS_1. 1 hit. PS50082. WD_REPEATS_2. 5 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	37534.
SOURCE	Search...

Entry information

Entry name

WDR1_HUMAN

Accession

Primary (citable) accession number: O75083
Secondary accession number(s): A8K6E9 , A8MPU4, O75313, Q8N6E5, Q9UG05, Q9UG78, Q9UQE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 121 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.