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Reviewed, UniProtKB/Swiss-Prot O75077 (ADA23_HUMAN)

Last modified November 25, 2008. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    ADAM 23
Alternative name(s):
    A disintegrin and metalloproteinase domain 23
    Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 3
      Short name=MDC-3
Gene names
Name: ADAM23
Synonyms: MDC3
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length832 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

May play a role in cell-cell and cell-matrix interactions. This is a non-catalytic metalloprotease-like protein.

Subunit structure

Ligand for integrin alpha-V/beta-3.

Subcellular location

Cell membrane; Single-pass type I membrane proteinPotential.

Isoform Gamma: Secreted.

Tissue specificity

Highly expressed in the brain and weakly expressed in the heart. In the brain, expressed prominently in the amygdala, caudate nucleus, hypothalamus, thalamus, cerebral cortex and occipital pole.

Developmental stage

Highly expressed in the fetal brain.

Domain

A conserved motif AVN[ED]CD within the disintegrin-like domain could be involved in the binding to the integrin receptor.

Sequence similarities

Contains 1 disintegrin domain.

Contains 1 EGF-like domain.

Contains 1 peptidase M12B domain.

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform Alpha (identifier: O75077-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Beta (identifier: O75077-2)

The sequence of this isoform differs from the canonical sequence as follows:
     787-817: GPSATNLIIGSIAGAILVAAIVLGGTGWGFK → VNMATSRLIGAVAGTLLALGVIFGGTGWGIE
Isoform Gamma (identifier: O75077-3)

The sequence of this isoform differs from the canonical sequence as follows:
     787-832: GPSATNLIIG...RFDPTQQGPI → EMSRREGSIL...AAVLLELLSL

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 5959 Potential
Propeptide60 – 286227 Potential
PRO_0000029118
Chain287 – 832546ADAM 23
PRO_0000029119

Regions

Topological domain287 – 792506Extracellular Potential
Transmembrane793 – 81321 Potential
Topological domain814 – 83219Cytoplasmic Potential
Domain299 – 496198Peptidase M12B
Domain502 – 58887Disintegrin
Domain732 – 76938EGF-like
Region563 – 5686May bind the integrin receptor
Compositional bias589 – 61123Cys-rich

Amino acid modifications

Modified residue7071Phosphotyrosine
Glycosylation761N-linked (GlcNAc...) Potential
Glycosylation961N-linked (GlcNAc...) Potential
Glycosylation1001N-linked (GlcNAc...) Potential
Glycosylation2631N-linked (GlcNAc...) Potential
Glycosylation5471N-linked (GlcNAc...) Potential
Glycosylation5481N-linked (GlcNAc...) Potential
Glycosylation6641N-linked (GlcNAc...) Potential
Glycosylation7321N-linked (GlcNAc...) Potential
Disulfide bond408 ↔ 491 By similarity
Disulfide bond450 ↔ 475 By similarity
Disulfide bond452 ↔ 459 By similarity
Disulfide bond560 ↔ 580 By similarity
Disulfide bond736 ↔ 751 By similarity
Disulfide bond745 ↔ 757 By similarity
Disulfide bond759 ↔ 768 By similarity

Natural variations

Alternative sequence787 – 83246GPSAT…QQGPI → EMSRREGSILLSKAPSESAA LDGHRLALLDSGYDILAAVL LELLSL in isoform Gamma.
VSP_012045
Alternative sequence787 – 81731GPSAT…GWGFK → VNMATSRLIGAVAGTLLALG VIFGGTGWGIE in isoform Beta.
VSP_012046

Experimental info

Mutagenesis5661E → A: Significantly lower of adhesion-promoting activity

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Sequences

Sequence LengthMass (Da)Tools
Isoform Alpha [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: 7841A9670E1C24EF

FASTA83291,926
        10         20         30         40         50         60 
MKPPGSSSRQ PPLAGCSLAG ASCGPQRGPA GSVPASAPAR TPPCRLLLVL LLLPPLAASS 

        70         80         90        100        110        120 
RPRAWGAAAP SAPHWNETAE KNLGVLADED NTLQQNSSSN ISYSNAMQKE ITLPSRLIYY 

       130        140        150        160        170        180 
INQDSESPYH VLDTKARHQQ KHNKAVHLAQ ASFQIEAFGS KFILDLILNN GLLSSDYVEI 

       190        200        210        220        230        240 
HYENGKPQYS KGGEHCYYHG SIRGVKDSKV ALSTCNGLHG MFEDDTFVYM IEPLELVHDE 

       250        260        270        280        290        300 
KSTGRPHIIQ KTLAGQYSKQ MKNLTMERGD QWPFLSELQW LKRRKRAVNP SRGIFEEMKY 

       310        320        330        340        350        360 
LELMIVNDHK TYKKHRSSHA HTNNFAKSVV NLVDSIYKEQ LNTRVVLVAV ETWTEKDQID 

       370        380        390        400        410        420 
ITTNPVQMLH EFSKYRQRIK QHADAVHLIS RVTFHYKRSS LSYFGGVCSR TRGVGVNEYG 

       430        440        450        460        470        480 
LPMAVAQVLS QSLAQNLGIQ WEPSSRKPKC DCTESWGGCI MEETGVSHSR KFSKCSILEY 

       490        500        510        520        530        540 
RDFLQRGGGA CLFNRPTKLF EPTECGNGYV EAGEECDCGF HVECYGLCCK KCSLSNGAHC 

       550        560        570        580        590        600 
SDGPCCNNTS CLFQPRGYEC RDAVNECDIT EYCTGDSGQC PPNLHKQDGY ACNQNQGRCY 

       610        620        630        640        650        660 
NGECKTRDNQ CQYIWGTKAA GSDKFCYEKL NTEGTEKGNC GKDGDRWIQC SKHDVFCGFL 

       670        680        690        700        710        720 
LCTNLTRAPR IGQLQGEIIP TSFYHQGRVI DCSGAHVVLD DDTDVGYVED GTPCGPSMMC 

       730        740        750        760        770        780 
LDRKCLQIQA LNMSSCPLDS KGKVCSGHGV CSNEATCICD FTWAGTDCSI RDPVRNLHPP 

       790        800        810        820        830 
KDEGPKGPSA TNLIIGSIAG AILVAAIVLG GTGWGFKNVK KRRFDPTQQG PI

« Hide

Isoform Beta [UniParc].

Checksum: E388ACE51334052C
Show »

83292,046
Isoform Gamma [UniParc].

Checksum: 7F54A1805FA2EC58
Show »

83292,130

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References

« Hide 'large scale' references
[1]"Metalloproteinase-like, disintegrin-like, cysteine-rich proteins MDC2 and MDC3: novel human cellular disintegrins highly expressed in the brain."
Sagane K., Ohya Y., Hasegawa Y., Tanaka I.
Biochem. J. 334:93-98(1998) [PubMed: 9693107] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA).
Tissue: Brain.
[2]"ADAM 23/MDC3, a human disintegrin that promotes cell adhesion via interaction with the alpha v beta 3 integrin through an RGD-independent mechanism."
Cal S., Freije J.M.P., Lopez J.M., Takada Y., Lopez-Otin C.
Mol. Biol. Cell 11:1457-1469(2000) [PubMed: 10749942] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA), INTERACTION WITH INTEGRIN ALPHA-V/BETA-3, MUTAGENESIS OF GLU-566.
Tissue: Brain.
[3]"Two novel isoforms of Adam23 expressed in the developmental process of mouse and human brains."
Sun Y.P., Deng K.J., Wang F., Zhang J., Huang X., Qiao S., Zhao S.
Gene 325:171-178(2004) [PubMed: 14697522] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA; BETA AND GAMMA), TISSUE SPECIFICITY.
Tissue: Fetal brain.
[4]"Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry."
Tao W.A., Wollscheid B., O'Brien R., Eng J.K., Li X.-J., Bodenmiller B., Watts J.D., Hood L., Aebersold R.
Nat. Methods 2:591-598(2005) [PubMed: 16094384] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-707, MASS SPECTROMETRY.
Tissue: T-cell.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AB009672 mRNA. Translation: BAA32351.1.
AJ005580 mRNA. Translation: CAC20565.1.
RefSeqNP_003803.1.
UniGeneHs.591643

3D structure databases

HSSPHSSP built from PDB template 1FVL based on UniProtKB P18619.
ModBaseSearch...

Protein family/group databases

MEROPSM12.979.

PTM databases

PhosphoSiteO75077.

Genome annotation databases

EnsemblENSG00000114948. Homo sapiens. [Contig view]
GeneID8745.
KEGGhsa:8745.

Organism-specific databases

H-InvDBHIX0002773.
HIX0030166.
HGNCHGNC:202. ADAM23.
MIM603710. gene.
PharmGKBPA24519.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMO75077.
HOVERGENO75077.

Gene expression databases

ArrayExpressO75077.
CleanExHS_ADAM23.
GermOnlineENSG00000114948. Homo sapiens.

Family and domain databases

InterProIPR006586. ADAM_cysteine.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR006210. EGF.
IPR000742. EGF_3.
IPR013111. EGF_extracell.
IPR013032. EGF_like_reg_CS.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
Gene3DG3DSA:4.10.70.10. Blood-coag_inhib_Disintegrin. 1 hit.
PfamPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF07974. EGF_2. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
ProDomPD000664. Disintegrin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
SM00181. EGF. 1 hit.
[Graphical view]
PROSITEPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. False negative.
PS50214. DISINTEGRIN_2. 1 hit.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. False negative.
PS50026. EGF_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio32813.
SOURCESearch...

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Entry information

Entry nameADA23_HUMAN
AccessionPrimary (citable) accession number: O75077
Entry history
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: November 1, 1998
Last modified: November 25, 2008
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents