O75063 (XYLK_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycosaminoglycan xylosylkinase
EC=2.7.1.-

Alternative name(s):
Protein FAM20B
Xylose kinase

Gene names

Name:	FAM20B
Synonyms:	KIAA0475

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Responsible for the 2-O-phosphorylation of xylose in the glycosaminoglycan-protein linkage region of proteoglycans thereby regulating the amount of mature GAG chains. Sulfated glycosaminoglycans (GAGs), including heparan sulfate and chondroitin sulfate, are synthesized on the so-called common GAG-protein linkage region (GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser) of core proteins, which is formed by the stepwise addition of monosaccharide residues by the respective specific glycosyltransferases. Xylose 2-o-phosphorylation may influence the catalytic activity of B3GAT3 (GlcAT-I) which completes the precursor tetrasaccharide of GAG-protein linkage regions on which the repeating disaccharide region is synthesized. Ref.1
Catalytic activity	ATP + D-xylose = ADP + D-xylose 2-phosphate. Ref.1
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Ref.1.
Tissue specificity	Widely expressed. Ref.7
Sequence similarities	Belongs to the FAM20 family.
Sequence caution	The sequence BAA32320.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW phosphotransferase activity, alcohol group as acceptor Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 409	409	Glycosaminoglycan xylosylkinase		PRO_0000008745
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 25	19	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	26 – 409	384	Lumenal Potential
Amino acid modifications
	Glycosylation	193	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O75063 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 03E211C0AF37B766	FASTA	409	46,432
	10 20 30 40 50 60 MKLKQRVVLL AILLVIFIFT KVFLIDNLDT SAANREDQRA FHRMMTGLRV ELAPKLDHTL 70 80 90 100 110 120 QSPWEIAAQW VVPREVYPEE TPELGAVMHA MATKKIIKAD VGYKGTQLKA LLILEGGQKV 130 140 150 160 170 180 VFKPKRYSRD HVVEGEPYAG YDRHNAEVAA FHLDRILGFH RAPLVVGRFV NLRTEIKPVA 190 200 210 220 230 240 TEQLLSTFLT VGNNTCFYGK CYYCRETEPA CADGDIMEGS VTLWLPDVWP LQKHRHPWGR 250 260 270 280 290 300 TYREGKLARW EYDESYCDAV KKTSPYDSGP RLLDIIDTAV FDYLIGNADR HHYESFQDDE 310 320 330 340 350 360 GASMLILLDN AKSFGNPSLD ERSILAPLYQ CCIIRVSTWN RLNYLKNGVL KSALKSAMAH 370 380 390 400 DPISPVLSDP HLDAVDQRLL SVLATVKQCT DQFGMDTVLV EDRMPLSHL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"FAM20B is a kinase that phosphorylates xylose in the glycosaminoglycan-protein linkage region." Koike T., Izumikawa T., Tamura J., Kitagawa H. Biochem. J. 421:157-162(2009) [PubMed: 19473117] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, CATALYTIC ACTIVITY, FUNCTION.
[2]	"Characterization of cDNA clones in size-fractionated cDNA libraries from human brain." Seki N., Ohira M., Nagase T., Ishikawa K., Miyajima N., Nakajima D., Nomura N., Ohara O. DNA Res. 4:345-349(1997) [PubMed: 9455484] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Hippocampus.
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Eye.
[7]	"FAM20: an evolutionarily conserved family of secreted proteins expressed in hematopoietic cells." Nalbant D., Youn H., Nalbant S.I., Sharma S., Cobos E., Beale E.G., Du Y., Williams S.C. BMC Genomics 6:11-11(2005) [PubMed: 15676076] [Abstract] Cited for: TISSUE SPECIFICITY.
[8]	"2-o-phosphorylation of xylose and 6-o-sulfation of galactose in the protein linkage region of glycosaminoglycans influence the glucuronyltransferase-I activity involved in the linkage region synthesis." Tone Y., Pedersen L.C., Yamamoto T., Izumikawa T., Kitagawa H., Nishihara J., Tamura J., Negishi M., Sugahara K. J. Biol. Chem. 283:16801-16807(2008) [PubMed: 18400750] [Abstract] Cited for: ROLE OF XYLOSE 2-O-PHOSPHORYLATION IN GAG BIOSYNTHESIS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB480690 mRNA. Translation: BAH79819.1. AB007944 mRNA. Translation: BAA32320.2. Different initiation. AK289989 mRNA. Translation: BAF82678.1. AL139132 Genomic DNA. Translation: CAH70923.1. AL139132 Genomic DNA. Translation: CAH70924.1. CH471067 Genomic DNA. Translation: EAW91034.1. BC046441 mRNA. Translation: AAH46441.1. BC051794 mRNA. Translation: AAH51794.1.
IPI	IPI00006657.
RefSeq	NP_055679.1. NM_014864.3.
UniGene	Hs.5737.
3D structure databases
ProteinModelPortal	O75063.
ModBase	Search...
Protein-protein interaction databases
STRING	O75063.
PTM databases
PhosphoSite	O75063.
Proteomic databases
PRIDE	O75063.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000263733; ENSP00000263733; ENSG00000116199. ENST00000440702; ENSP00000404005; ENSG00000116199.
GeneID	9917.
KEGG	hsa:9917.
UCSC	uc001gmc.1. human.
Organism-specific databases
CTD	9917.
GeneCards	GC01P178995.
H-InvDB	HIX0001372.
HGNC	HGNC:23017. FAM20B.
HPA	HPA007409.
MIM	611063. gene.
neXtProt	NX_O75063.
PharmGKB	PA134930918.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG07068.
GeneTree	ENSGT00390000007484.
HOGENOM	HBG715211.
HOVERGEN	HBG051635.
InParanoid	O75063.
OMA	KLARWEY.
OrthoDB	EOG44F696.
PhylomeDB	O75063.
Gene expression databases
ArrayExpress	O75063.
Bgee	O75063.
CleanEx	HS_FAM20B.
Genevestigator	O75063.
GermOnline	ENSG00000116199. Homo sapiens.
Family and domain databases
InterPro	IPR009581. DUF1193. IPR024869. FAM20. [Graphical view]
PANTHER	PTHR12450. DUF1193. 1 hit.
Pfam	PF06702. DUF1193. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	37408.
SOURCE	Search...

Entry information

Entry name

XYLK_HUMAN

Accession

Primary (citable) accession number: O75063
Secondary accession number(s): Q5W0C3, Q5W0C4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2003
Last sequence update:	November 1, 1998
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.