Reviewed, UniProtKB/Swiss-Prot O75052 (CAPON_HUMAN)

Names and origin

Protein names

Recommended name:
    Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein
Alternative name(s):
    C--terminal PDZ ligand of neuronal nitric oxide synthase protein
    Nitric oxide synthase 1 adaptor protein

Gene names

Name:	NOS1AP
Synonyms:	CAPON, KIAA0464

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Adapter protein involved in neuronal nitric-oxide (NO) synthesis regulation via its association with nNOS/NOS1. The complex formed with NOS1 and synapsins is necessary for specific NO and synapsin functions at a presynaptic level. Mediates an indirect interaction between NOS1 and RASD1 leading to enhance the ability of NOS1 to activate RASD1. Competes with DLG4 for interaction with NOS1, possibly affecting NOS1 activity by regulating the interaction between NOS1 and DLG4 By similarity.
Subunit structure	Interacts with the PDZ domain of NOS1 or the second PDZ domain of DLG4 through its C-terminus. Interacts with RASD1 and SYN1, SYN2 and SYN3 via its PID domain. Forms a ternary complex with NOS1 and RASD1. Forms a ternary complex with NOS1 and SYN1 By similarity.
Polymorphism	Genetic variation in NOS1AP influences the electrocardiographic QT interval [MIM:610141]. The QT interval is defined as the time from the beginning of the Q wave to the end of the T wave, representing the duration of ventricular electrical activity. The QT interval, a measure of cardiac repolarization, is a genetically influenced quantitative trait with considerable medical relevance: both high and low values are associated with increased risk of cardiovascular morbidity and mortality.
Sequence similarities	Contains 1 PID domain.

Ontologies

Keywords
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	PDZ domain binding Ref.4 Inferred from sequence or structural similarity. Source: UniProtKB nitric-oxide synthase binding Ref.4 Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PTPRN	Q16849	1	EBI-780467,EBI-728153

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein		PRO_0000089316
Regions
	Domain	26 – 196	171	PID
	Region	494 – 506	13	Interaction with NOS1 By similarity
	Coiled coil	322 – 363	42	Potential
	Motif	504 – 506	3	PDZ-binding By similarity
	Compositional bias	301 – 308	8	Poly-Gln
Amino acid modifications
	Modified residue	188	1	Phosphoserine By similarity
	Modified residue	192	1	Phosphoserine By similarity
	Modified residue	266	1	Phosphoserine Ref.6 Ref.7
	Modified residue	371	1	Phosphoserine Ref.8
	Modified residue	374	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	O75052-1 [UniParc]. Last modified January 4, 2005. Version 3. Checksum: D969C65E87684A7C	FASTA	506	56,150
	10 20 30 40 50 60 MPSKTKYNLV DDGHDLRIPL HNEDAFQHGI CFEAKYVGSL DVPRPNSRVE IVAAMRRIRY 70 80 90 100 110 120 EFKAKNIKKK KVSIMVSVDG VKVILKKKKK LLLLQKKEWT WDESKMLVMQ DPIYRIFYVS 130 140 150 160 170 180 HDSQDLKIFS YIARDGASNI FRCNVFKSKK KSQAMRIVRT VGQAFEVCHK LSLQHTQQNA 190 200 210 220 230 240 DGQEDGESER NSNSSGDPGR QLTGAERAST ATAEETDIDA VEVPLPGNDV LEFSRGVTDL 250 260 270 280 290 300 DAVGKEGGSH TGSKVSHPQE PMLTASPRML LPSSSSKPPG LGTETPLSTH HQMQLLQQLL 310 320 330 340 350 360 QQQQQQTQVA VAQVHLLKDQ LAAEAAARLE AQARVHQLLL QNKDMLQHIS LLVKQVQELE 370 380 390 400 410 420 LKLSGQNAMG SQDSLLEITF RSGALPVLCD PTTPKPEDLH SPPLGAGLAD FAHPAGSPLG 430 440 450 460 470 480 RRDCLVKLEC FRFLPPEDTP PPAQGEALLG GLELIKFRES GIASEYESNT DESEERDSWS 490 500 QEELPRLLNV LQRQELGDGL DDEIAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of cDNA clones in size-fractionated cDNA libraries from human brain." Seki N., Ohira M., Nagase T., Ishikawa K., Miyajima N., Nakajima D., Nomura N., Ohara O. DNA Res. 4:345-349(1997) [PubMed: 9455484] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"CAPON: a protein associated with neuronal nitric oxide synthase that regulates its interactions with PSD95." Jaffrey S.R., Snowman A.M., Eliasson M.J.L., Cohen N.A., Snyder S.H. Neuron 20:115-124(1998) [PubMed: 9459447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 354-506, INTERACTION WITH NOS1.
[5]	"A common genetic variant in the NOS1 regulator NOS1AP modulates cardiac repolarization." Arking D.E., Pfeufer A., Post W., Kao W.H.L., Newton-Cheh C., Ikeda M., West K., Kashuk C., Akyol M., Perz S., Jalilzadeh S., Illig T., Gieger C., Guo C.-Y., Larson M.G., Wichmann H.E., Marban E., O'Donnell C.J. , Hirschhorn J.N., Kaeaeb S., Spooner P.M., Meitinger T., Chakravarti A. Nat. Genet. 38:644-651(2006) [PubMed: 16648850] [Abstract] Cited for: INVOLVEMENT IN THE REGULATION OF QT INTERVALS.
[6]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-266, MASS SPECTROMETRY.
[7]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-266, MASS SPECTROMETRY.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-371 AND SER-374, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB007933 mRNA. Translation: BAA32309.2. Different initiation. AL590408, AL450163, AL512785 Genomic DNA. Translation: CAH71497.1. AL450163, AL590408, AL512785 Genomic DNA. Translation: CAH73230.1. AL512785, AL590408, AL450163 Genomic DNA. Translation: CAI15779.1. BC112295 mRNA. Translation: AAI12296.1. AF037070 mRNA. Translation: AAC39656.1.
IPI	IPI00514825.
RefSeq	NP_055512.1.
UniGene	Hs.655000
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O75052. 1 interaction.
STRING	O75052.
PTM databases
PhosphoSite	O75052.
Proteomic databases
PeptideAtlas	O75052.
PRIDE	O75052.
Genome annotation databases
Ensembl	ENST00000361897; ENSP00000355133; ENSG00000198929; Homo sapiens. [Genome view]
GeneID	9722.
UCSC	uc001gbv.2. human.
Organism-specific databases
CTD	9722.
GeneCards	GC01P160306.
H-InvDB	HIX0001257.
HGNC	HGNC:16859. NOS1AP.
HPA	CAB018582.
MIM	605551. gene. 610141. phenotype.
PharmGKB	PA142671259.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O75052.
HOVERGEN	O75052.
OMA	SKVSPHP
OrthoDB	EOG96HJWH
Gene expression databases
ArrayExpress	O75052.
Bgee	O75052.
CleanEx	HS_NOS1AP.
Genevestigator	O75052.
GermOnline	ENSG00000198929. Homo sapiens.
Family and domain databases
InterPro	IPR011993. PH_type. IPR006020. PTB_PID. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
Pfam	PF00640. PID. 1 hit. [Graphical view]
SMART	SM00462. PTB. 1 hit. [Graphical view]
PROSITE	PS01179. PID. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	36573.
SOURCE	Search...

Entry information

Entry name

CAPON_HUMAN

Accession

Primary (citable) accession number: O75052
Secondary accession number(s): O43564, Q5VU95

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	January 4, 2005
Last modified:	November 24, 2009
This is version 62 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.