Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O75052 (CAPON_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein
Alternative name(s):
C-terminal PDZ ligand of neuronal nitric oxide synthase protein
Nitric oxide synthase 1 adaptor protein
Gene names
Name:NOS1AP
Synonyms:CAPON, KIAA0464
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length506 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Adapter protein involved in neuronal nitric-oxide (NO) synthesis regulation via its association with nNOS/NOS1. The complex formed with NOS1 and synapsins is necessary for specific NO and synapsin functions at a presynaptic level. Mediates an indirect interaction between NOS1 and RASD1 leading to enhance the ability of NOS1 to activate RASD1. Competes with DLG4 for interaction with NOS1, possibly affecting NOS1 activity by regulating the interaction between NOS1 and DLG4 By similarity.

Subunit structure

Interacts with the PDZ domain of NOS1 or the second PDZ domain of DLG4 through its C-terminus. Interacts with RASD1 and SYN1, SYN2 and SYN3 via its PID domain. Forms a ternary complex with NOS1 and RASD1. Forms a ternary complex with NOS1 and SYN1 By similarity. Ref.5

Polymorphism

Genetic variation in NOS1AP influences the electrocardiographic QT interval [MIM:610141]. The QT interval is defined as the time from the beginning of the Q wave to the end of the T wave, representing the duration of ventricular electrical activity. The QT interval, a measure of cardiac repolarization, is a genetically influenced quantitative trait with considerable medical relevance: both high and low values are associated with increased risk of cardiovascular morbidity and mortality.

Sequence similarities

Contains 1 PID domain.

Sequence caution

The sequence BAA32309.2 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O75052-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O75052-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: MPSKTKYNLVDDGHDLR → MSLSSLCPVFSAAASSL
     18-312: Missing.
Isoform 3 (identifier: O75052-3)

The sequence of this isoform differs from the canonical sequence as follows:
     91-95: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 506506Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein
PRO_0000089316

Regions

Domain26 – 196171PID
Region494 – 50613Interaction with NOS1 By similarity
Coiled coil322 – 36342 Potential
Motif504 – 5063PDZ-binding By similarity
Compositional bias301 – 3088Poly-Gln

Amino acid modifications

Modified residue1881Phosphoserine By similarity
Modified residue1921Phosphoserine By similarity
Modified residue1951Phosphoserine By similarity
Modified residue2661Phosphoserine Ref.10
Modified residue3741Phosphoserine By similarity

Natural variations

Alternative sequence1 – 1717MPSKT…GHDLR → MSLSSLCPVFSAAASSL in isoform 2.
VSP_042751
Alternative sequence18 – 312295Missing in isoform 2.
VSP_042752
Alternative sequence91 – 955Missing in isoform 3.
VSP_043350

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 4, 2005. Version 3.
Checksum: D969C65E87684A7C

FASTA50656,150
        10         20         30         40         50         60 
MPSKTKYNLV DDGHDLRIPL HNEDAFQHGI CFEAKYVGSL DVPRPNSRVE IVAAMRRIRY 

        70         80         90        100        110        120 
EFKAKNIKKK KVSIMVSVDG VKVILKKKKK LLLLQKKEWT WDESKMLVMQ DPIYRIFYVS 

       130        140        150        160        170        180 
HDSQDLKIFS YIARDGASNI FRCNVFKSKK KSQAMRIVRT VGQAFEVCHK LSLQHTQQNA 

       190        200        210        220        230        240 
DGQEDGESER NSNSSGDPGR QLTGAERAST ATAEETDIDA VEVPLPGNDV LEFSRGVTDL 

       250        260        270        280        290        300 
DAVGKEGGSH TGSKVSHPQE PMLTASPRML LPSSSSKPPG LGTETPLSTH HQMQLLQQLL 

       310        320        330        340        350        360 
QQQQQQTQVA VAQVHLLKDQ LAAEAAARLE AQARVHQLLL QNKDMLQHIS LLVKQVQELE 

       370        380        390        400        410        420 
LKLSGQNAMG SQDSLLEITF RSGALPVLCD PTTPKPEDLH SPPLGAGLAD FAHPAGSPLG 

       430        440        450        460        470        480 
RRDCLVKLEC FRFLPPEDTP PPAQGEALLG GLELIKFRES GIASEYESNT DESEERDSWS 

       490        500 
QEELPRLLNV LQRQELGDGL DDEIAV 

« Hide

Isoform 2 [UniParc].

Checksum: E92ED7BE06D43943
Show »

FASTA21122,955
Isoform 3 [UniParc].

Checksum: 365A1A9B987EE0F3
Show »

FASTA50155,569

References

« Hide 'large scale' references
[1]"Characterization of cDNA clones in size-fractionated cDNA libraries from human brain."
Seki N., Ohira M., Nagase T., Ishikawa K., Miyajima N., Nakajima D., Nomura N., Ohara O.
DNA Res. 4:345-349(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[2]"Increased expression in dorsolateral prefrontal cortex of CAPON in schizophrenia and bipolar disorder."
Xu B., Wratten N., Charych E.I., Buyske S., Firestein B.L., Brzustowicz L.M.
PLoS Med. 2:E263-E263(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING.
[3]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Tissue: Brain.
[5]"CAPON: a protein associated with neuronal nitric oxide synthase that regulates its interactions with PSD95."
Jaffrey S.R., Snowman A.M., Eliasson M.J.L., Cohen N.A., Snyder S.H.
Neuron 20:115-124(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 354-506 (ISOFORM 1), INTERACTION WITH NOS1.
[6]"A common genetic variant in the NOS1 regulator NOS1AP modulates cardiac repolarization."
Arking D.E., Pfeufer A., Post W., Kao W.H.L., Newton-Cheh C., Ikeda M., West K., Kashuk C., Akyol M., Perz S., Jalilzadeh S., Illig T., Gieger C., Guo C.-Y., Larson M.G., Wichmann H.E., Marban E., O'Donnell C.J. expand/collapse author list , Hirschhorn J.N., Kaeaeb S., Spooner P.M., Meitinger T., Chakravarti A.
Nat. Genet. 38:644-651(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INVOLVEMENT IN THE REGULATION OF QT INTERVALS.
[7]"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-266, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB007933 mRNA. Translation: BAA32309.2. Different initiation.
AY841899 mRNA. Translation: AAW57298.1.
AL590408, AL450163, AL512785 Genomic DNA. Translation: CAH71497.1.
AL450163, AL590408, AL512785 Genomic DNA. Translation: CAH73230.1.
AL512785, AL590408, AL450163 Genomic DNA. Translation: CAI15779.1.
BC112295 mRNA. Translation: AAI12296.1.
BC143771 mRNA. Translation: AAI43772.1.
AF037070 mRNA. Translation: AAC39656.1.
RefSeqNP_001119532.2. NM_001126060.1.
NP_001158229.1. NM_001164757.1.
NP_055512.1. NM_014697.2.
UniGeneHs.731942.

3D structure databases

ProteinModelPortalO75052.
SMRO75052. Positions 29-167.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115071. 9 interactions.
IntActO75052. 1 interaction.
STRING9606.ENSP00000355133.

PTM databases

PhosphoSiteO75052.

Proteomic databases

PaxDbO75052.
PeptideAtlasO75052.
PRIDEO75052.

Protocols and materials databases

DNASU9722.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000361897; ENSP00000355133; ENSG00000198929. [O75052-1]
ENST00000493151; ENSP00000434988; ENSG00000198929. [O75052-2]
ENST00000530878; ENSP00000431586; ENSG00000198929. [O75052-3]
GeneID9722.
KEGGhsa:9722.
UCSCuc001gbv.2. human. [O75052-1]
uc001gbw.2. human. [O75052-3]
uc009wut.1. human. [O75052-2]

Organism-specific databases

CTD9722.
GeneCardsGC01P162039.
HGNCHGNC:16859. NOS1AP.
HPACAB018582.
MIM605551. gene.
610141. phenotype.
neXtProtNX_O75052.
Orphanet101016. Romano-Ward syndrome.
PharmGKBPA142671259.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG244289.
HOGENOMHOG000111298.
HOVERGENHBG050788.
InParanoidO75052.
KOK16513.
OMAQNTMGSQ.
OrthoDBEOG7JDQXM.
PhylomeDBO75052.
TreeFamTF317226.

Gene expression databases

ArrayExpressO75052.
BgeeO75052.
CleanExHS_NOS1AP.
GenevestigatorO75052.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
InterProIPR011993. PH_like_dom.
IPR006020. PTB/PI_dom.
[Graphical view]
PfamPF00640. PID. 1 hit.
[Graphical view]
SMARTSM00462. PTB. 1 hit.
[Graphical view]
PROSITEPS01179. PID. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiNOS1AP.
GenomeRNAi9722.
NextBio36573.
PROO75052.
SOURCESearch...

Entry information

Entry nameCAPON_HUMAN
AccessionPrimary (citable) accession number: O75052
Secondary accession number(s): B7ZLF5 expand/collapse secondary AC list , O43564, Q3T551, Q5VU95
Entry history
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 4, 2005
Last modified: April 16, 2014
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM