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O75022 (LIRB3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leukocyte immunoglobulin-like receptor subfamily B member 3
Short name=LIR-3
Short name=Leukocyte immunoglobulin-like receptor 3
Alternative name(s):
CD85 antigen-like family member A
Immunoglobulin-like transcript 5
Short name=ILT-5
Monocyte inhibitory receptor HL9
CD_antigen=CD85a
Gene names
Name:LILRB3
Synonyms:ILT5, LIR3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length631 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May act as receptor for class I MHC antigens. Becomes activated upon coligation of LILRB3 and immune receptors, such as FCGR2B and the B-cell receptor. Down-regulates antigen-induced B-cell activation by recruiting phosphatases to its immunoreceptor tyrosine-based inhibitor motifs (ITIM) By similarity.

Subunit structure

Interacts with LYN, PTPN6/SHP-1 and PTPN11/SHP-2 By similarity. Ref.5

Subcellular location

Cell membrane; Single-pass type I membrane protein.

Tissue specificity

Detected in monocytes and B-cells. Ref.2

Domain

Contains 3 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases, including PTPN6/SHP-1, resulting in the dephosphorylation of the downstream protein kinases SYK and BTK By similarity.

Post-translational modification

Phosphorylated on tyrosine residues by LYN. Phosphorylation at Tyr-595 and Tyr-625 is important for interaction with PTPN6/SHP-1 and PTPN11/SHP-2 By similarity.

Miscellaneous

Belongs to the leukocyte receptor cluster (LRC) present on 19q13.4.

Sequence similarities

Contains 4 Ig-like C2-type (immunoglobulin-like) domains.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O75022-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O75022-2)

The sequence of this isoform differs from the canonical sequence as follows:
     437-437: G → GGPEDQPLNPPGSGPQNG
Note: No experimental confirmation available.
Isoform 3 (identifier: O75022-3)

The sequence of this isoform differs from the canonical sequence as follows:
     530-530: S → SQ

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 631608Leukocyte immunoglobulin-like receptor subfamily B member 3
PRO_0000014822

Regions

Topological domain24 – 443420Extracellular Potential
Transmembrane444 – 46421Helical; Potential
Topological domain465 – 631167Cytoplasmic Potential
Domain42 – 10059Ig-like C2-type 1
Domain111 – 229119Ig-like C2-type 2
Domain225 – 31490Ig-like C2-type 3
Domain338 – 41982Ig-like C2-type 4
Motif512 – 5176ITIM motif 1
Motif593 – 5986ITIM motif 2
Motif623 – 6286ITIM motif 3

Amino acid modifications

Modified residue5951Phosphotyrosine; by LYN By similarity
Modified residue6251Phosphotyrosine; by LYN By similarity
Glycosylation1391N-linked (GlcNAc...) Potential
Glycosylation2801N-linked (GlcNAc...) Potential
Glycosylation3011N-linked (GlcNAc...) Potential
Glycosylation3401N-linked (GlcNAc...) Potential
Disulfide bond49 ↔ 98 By similarity
Disulfide bond144 ↔ 196 By similarity
Disulfide bond245 ↔ 296 Potential
Disulfide bond345 ↔ 396 Potential

Natural variations

Alternative sequence4371G → GGPEDQPLNPPGSGPQNG in isoform 2.
VSP_008459
Alternative sequence5301S → SQ in isoform 3.
VSP_040126
Natural variant211V → M.
Corresponds to variant rs1132588 [ dbSNP | Ensembl ].
VAR_017001
Natural variant591R → Q. Ref.2
Corresponds to variant rs678876 [ dbSNP | Ensembl ].
VAR_017002
Natural variant691L → W.
Corresponds to variant rs620207 [ dbSNP | Ensembl ].
VAR_017003
Natural variant901E → Q. Ref.2
Corresponds to variant rs1052963 [ dbSNP | Ensembl ].
VAR_017004
Natural variant1221S → N. Ref.1
Corresponds to variant rs3826750 [ dbSNP | Ensembl ].
VAR_017005
Natural variant2051W → Q Requires 2 nucleotide substitutions. Ref.1
Corresponds to variant rs1063805 [ dbSNP | Ensembl ].
VAR_017006
Natural variant4001Y → F.
Corresponds to variant rs8105096 [ dbSNP | Ensembl ].
VAR_017009
Natural variant4001Y → H.
Corresponds to variant rs1052992 [ dbSNP | Ensembl ].
VAR_017008
Natural variant4001Y → R Requires 2 nucleotide substitutions. Ref.2 Ref.3
VAR_017007
Natural variant4051H → Y. Ref.2
Corresponds to variant rs1132604 [ dbSNP | Ensembl ].
VAR_017010
Natural variant5391Q → H. Ref.2
Corresponds to variant rs1053002 [ dbSNP | Ensembl ].
VAR_017012
Natural variant5741V → A. Ref.2
Corresponds to variant rs1053008 [ dbSNP | Ensembl ].
VAR_017013

Experimental info

Sequence conflict531Q → L in AAB68668. Ref.1
Sequence conflict531Q → L in AAB87667. Ref.2
Sequence conflict611H → D in AAB68668. Ref.1
Sequence conflict611H → D in AAB87667. Ref.2
Sequence conflict1151M → L in AAB68668. Ref.1
Sequence conflict1151M → L in AAB87667. Ref.2
Sequence conflict1201A → F in AAB68668. Ref.1
Sequence conflict1491G → R in AAB87667. Ref.2
Sequence conflict1751R → G in AAB68668. Ref.1
Sequence conflict1751R → G in AAB87667. Ref.2
Sequence conflict1871T → N in AAB68668. Ref.1
Sequence conflict1871T → N in AAB87667. Ref.2
Sequence conflict1871T → N in AAP30716. Ref.3
Sequence conflict2011T → M in AAB68668. Ref.1
Sequence conflict2011T → M in AAB87667. Ref.2
Sequence conflict2051W → R in AAB87667. Ref.2
Sequence conflict2521N → D in AAB68668. Ref.1
Sequence conflict2521N → D in AAB87667. Ref.2
Sequence conflict2631D → A in AAP30716. Ref.3
Sequence conflict2681P → S in AAP30716. Ref.3
Sequence conflict2901N → H in AAB68668. Ref.1
Sequence conflict4091H → F in AAB68668. Ref.1
Sequence conflict4171V → M in AAB68668. Ref.1
Sequence conflict5251R → G in AAB87667. Ref.2
Sequence conflict5611S → P in AAB87667. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 30, 2010. Version 3.
Checksum: 595B1BD5283A7E3E

FASTA63169,386
        10         20         30         40         50         60 
MTPALTALLC LGLSLGPRTR VQAGPFPKPT LWAEPGSVIS WGSPVTIWCQ GSQEAQEYRL 

        70         80         90        100        110        120 
HKEGSPEPLD RNNPLEPKNK ARFSIPSMTE HHAGRYRCHY YSSAGWSEPS DPLEMVMTGA 

       130        140        150        160        170        180 
YSKPTLSALP SPVVASGGNM TLRCGSQKGY HHFVLMKEGE HQLPRTLDSQ QLHSRGFQAL 

       190        200        210        220        230        240 
FPVGPVTPSH RWRFTCYYYY TNTPWVWSHP SDPLEILPSG VSRKPSLLTL QGPVLAPGQS 

       250        260        270        280        290        300 
LTLQCGSDVG YNRFVLYKEG ERDFLQRPGQ QPQAGLSQAN FTLGPVSPSN GGQYRCYGAH 

       310        320        330        340        350        360 
NLSSEWSAPS DPLNILMAGQ IYDTVSLSAQ PGPTVASGEN VTLLCQSWWQ FDTFLLTKEG 

       370        380        390        400        410        420 
AAHPPLRLRS MYGAHKYQAE FPMSPVTSAH AGTYRCYGSY SSNPHLLSHP SEPLELVVSG 

       430        440        450        460        470        480 
HSGGSSLPPT GPPSTPGLGR YLEVLIGVSV AFVLLLFLLL FLLLRRQRHS KHRTSDQRKT 

       490        500        510        520        530        540 
DFQRPAGAAE TEPKDRGLLR RSSPAADVQE ENLYAAVKDT QSEDRVELDS QSPHDEDPQA 

       550        560        570        580        590        600 
VTYAPVKHSS PRREMASPPS SLSGEFLDTK DRQVEEDRQM DTEAAASEAS QDVTYAQLHS 

       610        620        630 
LTLRRKATEP PPSQEGEPPA EPSIYATLAI H

« Hide

Isoform 2 [UniParc].

Checksum: 3FAEB2EEC21105BD
Show »

FASTA64871,029
Isoform 3 [UniParc].

Checksum: FF29920A9E55897D
Show »

FASTA63269,514

References

« Hide 'large scale' references
[1]"Molecular identification of a novel family of human Ig superfamily members that possess immunoreceptor tyrosine-based inhibition motifs and homology to the mouse gp49B1 inhibitory receptor."
Arm J.P., Nwankwo C., Austen K.F.
J. Immunol. 159:2342-2349(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS ASN-122 AND GLN-205.
Tissue: Monocyte.
[2]"A family of human lymphoid and myeloid Ig-like receptors, some of which bind to MHC class I molecules."
Borges L., Hsu M.-L., Fanger N., Kubin M., Cosman D.
J. Immunol. 159:5192-5196(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS GLN-59; GLN-90; ARG-400; TYR-405; HIS-539 AND ALA-574, TISSUE SPECIFICITY.
Tissue: Peripheral blood leukocyte.
[3]"Genomics and diversity of the immunoglobulin-like transcript 5 locus."
Cuillerier B., Bahram S.
Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 2), VARIANT ARG-400.
[4]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"Extensive gene duplications and a large inversion characterize the human leukocyte receptor cluster."
Wende H., Volz A., Ziegler A.
Immunogenetics 51:703-713(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE LRC.
[6]Erratum
Wende H., Volz A., Ziegler A.
Immunogenetics 52:3-4(2001)
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U91928 mRNA. Translation: AAB68668.1.
AF025533 mRNA. Translation: AAB87667.1.
AF256195 Genomic DNA. Translation: AAP30716.1.
AC010492 Genomic DNA. No translation available.
AC012314 Genomic DNA. No translation available.
IPIIPI00376782.
IPI00893140.
IPI00938185.
RefSeqNP_001074919.1. NM_001081450.1.
NP_006855.2. NM_006864.2.
UniGeneHs.631592.

3D structure databases

ProteinModelPortalO75022.
SMRO75022. Positions 28-419.
ModBaseSearch...

Protein-protein interaction databases

IntActO75022. 1 interaction.
STRING9606.ENSP00000390120.

Protein family/group databases

MEROPSI43.001.

PTM databases

PhosphoSiteO75022.

Proteomic databases

PaxDbO75022.
PRIDEO75022.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000245620; ENSP00000245620; ENSG00000204577.
ENST00000391750; ENSP00000375630; ENSG00000204577.
ENST00000424807; ENSP00000412771; ENSG00000204577.
ENST00000570726; ENSP00000459919; ENSG00000262087.
ENST00000575296; ENSP00000461633; ENSG00000262087.
GeneID11025.
KEGGhsa:11025.
UCSCuc002qee.1. human.
uc002qef.1. human.

Organism-specific databases

CTD11025.
GeneCardsGC19M054720.
H-InvDBHIX0015438.
HGNCHGNC:6607. LILRB3.
MIM604820. gene.
neXtProtNX_O75022.
PharmGKBPA30381.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG25855.
HOGENOMHOG000234395.
HOVERGENHBG074353.
KOK06512.
OrthoDBEOG43R3MG.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.

Gene expression databases

ArrayExpressO75022.
BgeeO75022.
CleanExHS_LILRB3.
GenevestigatorO75022.
GermOnlineENSG00000186152. Homo sapiens.

Family and domain databases

Gene3D2.60.40.10. 4 hits.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
[Graphical view]
SMARTSM00409. IG. 2 hits.
[Graphical view]
PROSITEPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSLILRB3. human.
GenomeRNAi11025.
NextBio41896.
SOURCESearch...

Entry information

Entry nameLIRB3_HUMAN
AccessionPrimary (citable) accession number: O75022
Secondary accession number(s): C9J1P3 expand/collapse secondary AC list , C9JIP1, O15471, Q86U49
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: November 30, 2010
Last modified: April 3, 2013
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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