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Protein

Trehalose phosphorylase

Gene
N/A
Organism
Grifola frondosa (Maitake) (Polyporus frondosus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the synthesis and degradation of trehalose from glucose and alpha-D-glucose 1-phosphate. The equilibrium lies in the direction of trehalose synthesis.2 Publications

Catalytic activityi

Alpha,alpha-trehalose + phosphate = alpha-D-glucose + alpha-D-glucose 1-phosphate.2 Publications

Enzyme regulationi

Activity abolished by 1 mM Cu2+. 0.1 mM Cu2+ reduces trehalose phosphorolysis to 76% and trehalose synthesis to 48% of maximum activity. 1 mM Zn2+ abolishes trehalose synthesis, and reduces trehalose phosphorolysis to 40% of maximum activity. Unaffected by EDTA.1 Publication

pH dependencei

Optimum pH is 6.5 for trehalose phosphorolysis activity, 6.5-6.8 for trehalose synthesis activity.1 Publication

Temperature dependencei

Optimum temperature is 32.5 degrees Celsius for trehalose phosphorolysis activity, 37.5 degrees Celsius for trehalose synthesis activity. Stable up to 35 degrees Celsius for trehalose phosphorolysis activity, 32.5 degrees Celsius for trehalose synthesis activity.1 Publication

GO - Molecular functioni

  • alpha,alpha-trehalose phosphorylase (configuration-retaining) activity Source: UniProtKB

GO - Biological processi

  • glucose 6-phosphate metabolic process Source: UniProtKB
  • glucose metabolic process Source: UniProtKB
  • trehalose metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-5943.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
Trehalose phosphorylaseBy similarity (EC:2.4.1.231)
Alternative name(s):
Trehalose synthaseImported
Short name:
TSase2 Publications
OrganismiGrifola frondosa (Maitake) (Polyporus frondosus)
Taxonomic identifieri5627 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesSchizophyllaceaeGrifola

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00004052931 – 251 PublicationAdd BLAST25
ChainiPRO_000040529426 – 732Trehalose phosphorylase1 PublicationAdd BLAST707

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO75003.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O75003-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPPHQFQSK PSDVIRRRLS SAVSSKRPNI PGYTSLTPMW AGIAGAVVNN
60 70 80 90 100
NTQFEVAISI HDSVYNTDFA SSVVPYSPNE PEAQAGIIEK HVLETLRKFS
110 120 130 140 150
TEHMCKFLGA GVTVILLREA PNLCTRLWLD MDIVPIVFNI KPFHTDSITR
160 170 180 190 200
PNVRHRISST TGSYVPSGAE TPTVYYDPAQ LQDPNKLSAN VQTRLPIPRT
210 220 230 240 250
VDEQADSAAR KCIMYFGPGN NPRLQIGPRN QVAVDAGGKI HLIDDIDEYR
260 270 280 290 300
KTVGKGTWNS VIKLADELRE KKIKIGFFSS TPQGGGVALM RHAIIRFFTA
310 320 330 340 350
LDVDAAWYVP NPSPSVFRTT KNNHNILQGV ADPSLRLTKE AADNFDSWIL
360 370 380 390 400
KNGLRWTAEG GPLAPGGVDI AFIDDPQMPG LIPLIKRIRP DLPIIYRSHI
410 420 430 440 450
EIRSDLVHVK GSPQEEVWNY LWNNIQHSDL FISHPVNKFV PSDVPLEKLA
460 470 480 490 500
LLGAATDWLD GLSKHLDAWD SQYYMGEFRN LCVKEKMNEL GWPAREYIVQ
510 520 530 540 550
IARFDPSKGI PNVIDSYARF RKLCVDKVME DDIPQLLLCG HGAVDDPDAS
560 570 580 590 600
IIYDQVLQLI HAKYKEYAPD IVVMRCPPSD QLLNTLMANA KFALQLSTRE
610 620 630 640 650
GFEVKVSEAL HAGKPVIACR TGGIPLQIEH GKSGYLCEPG DNAAVAQHML
660 670 680 690 700
DLYTDEDLYD TMSEYARTHV SDEVGTVGNA AAWMYLAVMY VSRGVKLRPH
710 720 730
GAWINDLMRT EMGEPYRPGE PRLPRGELHV QG
Length:732
Mass (Da):81,582
Last modified:November 1, 1998 - v1
Checksum:i5E01041357118DAF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30I → V in ADM15725 (Ref. 2) Curated1
Sequence conflicti52T → S in ADM15725 (Ref. 2) Curated1
Sequence conflicti73V → I in ADM15725 (Ref. 2) Curated1
Sequence conflicti378M → V in ADM15725 (Ref. 2) Curated1
Sequence conflicti471S → A in ADM15725 (Ref. 2) Curated1
Sequence conflicti483V → I in ADM15725 (Ref. 2) Curated1
Sequence conflicti496E → D in ADM15725 (Ref. 2) Curated1
Sequence conflicti718P → A in ADM15725 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010104 Genomic DNA. Translation: BAA31349.1.
AB010105 mRNA. Translation: BAA31350.1.
HM631875 mRNA. Translation: ADM15725.1.
PIRiT00130.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010104 Genomic DNA. Translation: BAA31349.1.
AB010105 mRNA. Translation: BAA31350.1.
HM631875 mRNA. Translation: ADM15725.1.
PIRiT00130.

3D structure databases

ProteinModelPortaliO75003.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-5943.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTREPH_GRIFR
AccessioniPrimary (citable) accession number: O75003
Secondary accession number(s): E1ACW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: November 1, 1998
Last modified: November 11, 2015
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.