Reviewed, UniProtKB/Swiss-Prot O74991 (POF3_SCHPO)

Names and origin

Protein names

Recommended name:
    F-box/TPR repeat protein pof3

Gene names

Name:	pof3
ORF Names:	SPCC338.16

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has a role in substrate recognition in the Skp1-Cullin-1/Cdc53-F-box (SCF) ubiquitin ligase complex. Required for the maintenance of telomere length and transcriptional silencing at the telomere. Also required for chromosome segregation. Ref.1
Subunit structure	A part of the E3 ubiquitin ligase Skp1-Cullin-1-F-box (SCF) complex. Interacts with cul1, mcl1 and skp1. Ref.1 Ref.3 Ref.4
Subcellular location	Mitochondrion. Nucleus. Ref.1 Ref.5
Sequence similarities	Contains 1 F-box domain. Contains 3 TPR repeats.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Ubl conjugation pathway
Cellular component	Chromosomal protein Mitochondrion Nucleus
Domain	Repeat TPR repeat
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW telomere maintenance Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromosome Inferred from electronic annotation. Source: UniProtKB-KW mitochondrion Ref.5 Inferred from direct assay. Source: GeneDB_SPombe nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Ref.1 Ref.3 Ref.4 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
cul1	O13790	1	EBI-1153554,EBI-1154807
mcl1	Q9C107	1	EBI-1153554,EBI-1203666
SKP1	P52286	1	EBI-1153554,EBI-4090	From a different organism.
skp1	Q9Y709	2	EBI-1153554,EBI-1172248

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 577	577	F-box/TPR repeat protein pof3		PRO_0000106362
Regions
	Repeat	6 – 39	34	TPR 1
	Repeat	41 – 74	34	TPR 2
	Repeat	76 – 108	33	TPR 3
	Domain	138 – 180	43	F-box

Sequences

	Sequence		Length	Mass (Da)
	O74991-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 507D7901C1980D3F	FASTA	577	66,239
	10 20 30 40 50 60 MNNYQVKAIK EKTQQYLSKR KFEDALTFIT KTIEQEPNPT IDLFELRAQV YEKSGQYSQA 70 80 90 100 110 120 ELDAKRMIHL NARNARGYLR LGKLLQLDGF DKKADQLYTQ GLRMVHKMDP LRPVLKKVSQ 130 140 150 160 170 180 RLNERILRTR PVLDLFRILP REVLLCILQQ LNFKSIVQCM QVCKHWRDCI KKEPSLFCCL 190 200 210 220 230 240 DFSCASPRSV NSRDRNVMAV ARYSVYSKDN IQEVIGLEKL GILTPTKALL RSVKSLKVYK 250 260 270 280 290 300 TISPLHTQST DKLYTIWTPF SELHYFYCAT PITFSIASKI LSCCKKLKQV ELVDLIPDLI 310 320 330 340 350 360 FDSMDWDKLF NAESVPLALK SLTFIRNQKF PFHHKEQQFL KDLLSASPYL EYLEASYQSD 370 380 390 400 410 420 LVAAIKKYKI NLRSLIIIDE GVSNTVKDLA FLPQSLTTLI VKPCNPASTI LCPYLFPTNV 430 440 450 460 470 480 RMESLINLEL FLYLRLSQND IDNVVKFLTS CYKLKKLVLH DSLALAPHFF EIFASLPELE 490 500 510 520 530 540 HLEIPDNVAL QNKHAIHITD CCPNLKYVNF SNSISLDGSG FIAVLRGLKE LKRIDIINCD 550 560 570 SVSRDAIDWA RSKGMQVTVA SSLPNSQPLG TKKIRLI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Fission yeast F-box protein Pof3 is required for genome integrity and telomere function." Katayama S., Kitamura K., Lehmann A., Nikaido O., Toda T. Mol. Biol. Cell 13:211-224(2002) [PubMed: 11809834] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT, SUBCELLULAR LOCATION. Strain: ATCC 38366 / 972.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[3]	"Molecular interactions of fission yeast Skp1 and its role in the DNA damage checkpoint." Lehmann A., Katayama S., Harrison C., Dhut S., Kitamura K., McDonald N., Toda T. Genes Cells 9:367-382(2004) [PubMed: 15147268] [Abstract] Cited for: INTERACTION WITH SKP1.
[4]	"Fission yeast Mcl1 interacts with SCF(Pof3) and is required for centromere formation." Mamnun Y.M., Katayama S., Toda T. Biochem. Biophys. Res. Commun. 350:125-130(2006) [PubMed: 16997270] [Abstract] Cited for: INTERACTION WITH MCL1.
[5]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB032411 Genomic DNA. Translation: BAA84529.1. CU329672 Genomic DNA. Translation: CAA19347.1.
PIR	T41727.
RefSeq	NP_588152.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O74991. 4 interactions.
Genome annotation databases
GeneID	2539527.
KEGG	spo:SPCC338.16.
NMPDR	fig|4896.1.peg.490.
Organism-specific databases
GeneDB_Spombe	SPCC338.16.
Phylogenomic databases
OMA	O74991. RIDIINC.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-000092-MON.
Gene expression databases
ArrayExpress	O74991.
Family and domain databases
InterPro	IPR001810. F-box. IPR011990. TPR-like_helical. IPR013026. TPR_region. [Graphical view]
Gene3D	G3DSA:1.25.40.10. TPR-like_helical. 1 hit.
Pfam	PF00646. F-box. 1 hit. [Graphical view]
SMART	SM00256. FBOX. 1 hit. [Graphical view]
PROSITE	PS50181. FBOX. 1 hit. PS50005. TPR. False negative. PS50293. TPR_REGION. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POF3_SCHPO

Accession

Primary (citable) accession number: O74991

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	November 1, 1998
Last modified:	June 16, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)