O74991 (POF3_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
F-box/TPR repeat protein pof3

Gene names

Name:	pof3
ORF Names:	SPCC338.16

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has a role in substrate recognition in the Skp1-Cullin-1/Cdc53-F-box (SCF) ubiquitin ligase complex. Required for the maintenance of telomere length and transcriptional silencing at the telomere. Also required for chromosome segregation. Ref.1
Subunit structure	A part of the E3 ubiquitin ligase Skp1-Cullin-1-F-box (SCF) complex. Interacts with cul1, mcl1 and skp1. Ref.1 Ref.3 Ref.4
Subcellular location	Mitochondrion. Nucleus Ref.1 Ref.5.
Sequence similarities	Contains 1 F-box domain. Contains 3 TPR repeats.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Ubl conjugation pathway
Cellular component	Mitochondrion Nucleus
Domain	Repeat TPR repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of G2/M transition of mitotic cell cycle Inferred from mutant phenotype. Source: GeneDB_Spombe regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW telomere maintenance Inferred from mutant phenotype. Source: GeneDB_Spombe transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from direct assay. Source: GeneDB_Spombe nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction Ref.3. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
cul1	O13790	2	EBI-1153554,EBI-1154807
mcl1	Q9C107	2	EBI-1153554,EBI-1203666
skp1	Q9Y709	3	EBI-1153554,EBI-1172248

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 577	577	F-box/TPR repeat protein pof3		PRO_0000106362
Regions
	Repeat	6 – 39	34	TPR 1
	Repeat	41 – 74	34	TPR 2
	Repeat	76 – 108	33	TPR 3
	Domain	138 – 180	43	F-box

Sequences

	Sequence		Length	Mass (Da)
	O74991 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 507D7901C1980D3F	FASTA	577	66,239
	10 20 30 40 50 60 MNNYQVKAIK EKTQQYLSKR KFEDALTFIT KTIEQEPNPT IDLFELRAQV YEKSGQYSQA 70 80 90 100 110 120 ELDAKRMIHL NARNARGYLR LGKLLQLDGF DKKADQLYTQ GLRMVHKMDP LRPVLKKVSQ 130 140 150 160 170 180 RLNERILRTR PVLDLFRILP REVLLCILQQ LNFKSIVQCM QVCKHWRDCI KKEPSLFCCL 190 200 210 220 230 240 DFSCASPRSV NSRDRNVMAV ARYSVYSKDN IQEVIGLEKL GILTPTKALL RSVKSLKVYK 250 260 270 280 290 300 TISPLHTQST DKLYTIWTPF SELHYFYCAT PITFSIASKI LSCCKKLKQV ELVDLIPDLI 310 320 330 340 350 360 FDSMDWDKLF NAESVPLALK SLTFIRNQKF PFHHKEQQFL KDLLSASPYL EYLEASYQSD 370 380 390 400 410 420 LVAAIKKYKI NLRSLIIIDE GVSNTVKDLA FLPQSLTTLI VKPCNPASTI LCPYLFPTNV 430 440 450 460 470 480 RMESLINLEL FLYLRLSQND IDNVVKFLTS CYKLKKLVLH DSLALAPHFF EIFASLPELE 490 500 510 520 530 540 HLEIPDNVAL QNKHAIHITD CCPNLKYVNF SNSISLDGSG FIAVLRGLKE LKRIDIINCD 550 560 570 SVSRDAIDWA RSKGMQVTVA SSLPNSQPLG TKKIRLI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Fission yeast F-box protein Pof3 is required for genome integrity and telomere function." Katayama S., Kitamura K., Lehmann A., Nikaido O., Toda T. Mol. Biol. Cell 13:211-224(2002) [PubMed: 11809834] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT, SUBCELLULAR LOCATION. Strain: 972 / ATCC 24843.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[3]	"Molecular interactions of fission yeast Skp1 and its role in the DNA damage checkpoint." Lehmann A., Katayama S., Harrison C., Dhut S., Kitamura K., McDonald N., Toda T. Genes Cells 9:367-382(2004) [PubMed: 15147268] [Abstract] Cited for: INTERACTION WITH SKP1.
[4]	"Fission yeast Mcl1 interacts with SCF(Pof3) and is required for centromere formation." Mamnun Y.M., Katayama S., Toda T. Biochem. Biophys. Res. Commun. 350:125-130(2006) [PubMed: 16997270] [Abstract] Cited for: INTERACTION WITH MCL1.
[5]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB032411 Genomic DNA. Translation: BAA84529.1. CU329672 Genomic DNA. Translation: CAA19347.1.
PIR	T41727.
RefSeq	NP_588152.1. NM_001023141.1.
3D structure databases
ProteinModelPortal	O74991.
ModBase	Search...
Protein-protein interaction databases
IntAct	O74991. 4 interactions.
STRING	O74991.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPCC338.16.1; SPCC338.16.1:pep; SPCC338.16.
GeneID	2539527.
GenomeReviews	Gene locus pof3 in contig CU329672_GR.
KEGG	spo:SPCC338.16.
NMPDR	fig|4896.1.peg.490.
Organism-specific databases
GeneDB_Spombe	SPCC338.16.
Phylogenomic databases
eggNOG	fuNOG13293.
GeneTree	EFGT00050000006629.
OMA	SRDAIDW.
OrthoDB	EOG4004SM.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-000092-MONOMER.
Gene expression databases
ArrayExpress	O74991.
Family and domain databases
InterPro	IPR001810. F-box_dom_cyclin-like. IPR022364. F-box_dom_Skp2-like. IPR013026. TPR-contain. IPR011990. TPR-like_helical. [Graphical view]
Gene3D	G3DSA:1.25.40.10. TPR-like_helical. 1 hit.
KO	K15069.
Pfam	PF00646. F-box. 1 hit. [Graphical view]
SMART	SM00256. FBOX. 1 hit. [Graphical view]
SUPFAM	SSF81383. F-box_dom_Skp2-like. 1 hit.
PROSITE	PS50181. FBOX. 1 hit. PS50005. TPR. False negative. PS50293. TPR_REGION. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POF3_SCHPO

Accession

Primary (citable) accession number: O74991

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	November 1, 1998
Last modified:	November 16, 2011
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program