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Protein

Eukaryotic translation initiation factor 2A

Gene

SPBC4B4.04

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis, Translation regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 2A
Short name:
eIF-2A
Gene namesi
ORF Names:SPBC4B4.04
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC4B4.04.
PomBaseiSPBC4B4.04.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 576576Eukaryotic translation initiation factor 2APRO_0000286083Add
BLAST

Proteomic databases

MaxQBiO74965.

Interactioni

Protein-protein interaction databases

BioGridi277515. 174 interactions.
MINTiMINT-4682114.

Structurei

Secondary structure

1
576
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 138Combined sources
Beta strandi15 – 195Combined sources
Helixi20 – 223Combined sources
Beta strandi37 – 426Combined sources
Beta strandi46 – 5510Combined sources
Beta strandi57 – 615Combined sources
Turni62 – 643Combined sources
Beta strandi67 – 715Combined sources
Beta strandi75 – 817Combined sources
Beta strandi85 – 928Combined sources
Beta strandi106 – 1138Combined sources
Beta strandi116 – 1205Combined sources
Beta strandi138 – 1436Combined sources
Beta strandi146 – 1527Combined sources
Beta strandi160 – 1623Combined sources
Beta strandi170 – 1734Combined sources
Beta strandi176 – 1783Combined sources
Beta strandi180 – 1856Combined sources
Beta strandi193 – 2019Combined sources
Beta strandi203 – 21412Combined sources
Beta strandi220 – 2223Combined sources
Beta strandi228 – 2336Combined sources
Beta strandi242 – 2476Combined sources
Beta strandi267 – 2693Combined sources
Beta strandi271 – 2766Combined sources
Beta strandi283 – 29715Combined sources
Beta strandi299 – 3013Combined sources
Beta strandi303 – 3119Combined sources
Beta strandi314 – 3174Combined sources
Beta strandi321 – 3277Combined sources
Beta strandi330 – 3334Combined sources
Beta strandi335 – 3406Combined sources
Turni341 – 3444Combined sources
Beta strandi345 – 3528Combined sources
Beta strandi358 – 3614Combined sources
Beta strandi365 – 3728Combined sources
Turni374 – 3763Combined sources
Beta strandi382 – 3909Combined sources
Beta strandi392 – 3976Combined sources
Beta strandi399 – 4068Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3WJ9X-ray2.51A/B2-412[»]
ProteinModelPortaliO74965.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati71 – 11949WD 1Add
BLAST
Repeati266 – 30742WD 2Add
BLAST
Repeati308 – 34942WD 3Add
BLAST
Repeati351 – 39646WD 4Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat EIF2A family.Curated
Contains 4 WD repeats.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

HOGENOMiHOG000193775.
InParanoidiO74965.
KOiK15026.
OMAiCARVVTN.
OrthoDBiEOG092C2TXG.
PhylomeDBiO74965.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR011387. TIF2A.
IPR013979. TIF_beta_prop-like.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamiPF08662. eIF2A. 1 hit.
[Graphical view]
PIRSFiPIRSF017222. eIF2A. 1 hit.

Sequencei

Sequence statusi: Complete.

O74965-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQKSQFAYR SSKSIGLVNA SENYASPPKF EAISEPARNA CYSPNGKLFA
60 70 80 90 100
YATATQVVIN DTESGAKLTQ LPAANTYELG FSPLGKYLST WERPGKEADG
110 120 130 140 150
TPKQNMKVWN TETGQLVFSF VQRNQTGWNL QYTCDESLAA RLVTNEVHFY
160 170 180 190 200
ETGNMSKGPI AKLRVEGISD FALSPGQNHA VAVFIPEKKG APASVRTYSI
210 220 230 240 250
PNFNSPLSQK TFFKADKVQF KWNALGTSLL VLTQTEVDKS NKNYYGETNL
260 270 280 290 300
YLLGITGQFD CRVDLDREGP IHDVCWNADS KEFGIVYGYM PAKTAIFDNR
310 320 330 340 350
ANVVSIIPPA PRNTLIFSPN SRYILLAGFG NLQGSIDIFD AANNMKKITT
360 370 380 390 400
VEAANCTYCE FSPDSQFLLT AVTSPRLRVD NSIKIWHITG APMFYEEFNE
410 420 430 440 450
LYQAFWRPRP LNPTLLQNAL TSASLPSPPT PHASASKLAA KPSVKPAGAY
460 470 480 490 500
RPPGARGQNS TFSYRREEID VMSSGSANKH VNSSRQRVVP GATPVIDGNK
510 520 530 540 550
KNNKKTKPAV KQVAQPTAEV SDEKKIRSLC KKLRAIDDLK SRLNNNEKLE
560 570
ATQVKKIESE GKVLAELKAL GWTPDA
Length:576
Mass (Da):63,497
Last modified:November 1, 1998 - v1
Checksum:i6702ADCCAF5A2A62
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA19284.1.
PIRiT40476.
RefSeqiNP_596421.1. NM_001022340.2.

Genome annotation databases

EnsemblFungiiSPBC4B4.04.1; SPBC4B4.04.1:pep; SPBC4B4.04.
GeneIDi2540999.
KEGGispo:SPBC4B4.04.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA19284.1.
PIRiT40476.
RefSeqiNP_596421.1. NM_001022340.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3WJ9X-ray2.51A/B2-412[»]
ProteinModelPortaliO74965.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277515. 174 interactions.
MINTiMINT-4682114.

Proteomic databases

MaxQBiO74965.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC4B4.04.1; SPBC4B4.04.1:pep; SPBC4B4.04.
GeneIDi2540999.
KEGGispo:SPBC4B4.04.

Organism-specific databases

EuPathDBiFungiDB:SPBC4B4.04.
PomBaseiSPBC4B4.04.

Phylogenomic databases

HOGENOMiHOG000193775.
InParanoidiO74965.
KOiK15026.
OMAiCARVVTN.
OrthoDBiEOG092C2TXG.
PhylomeDBiO74965.

Miscellaneous databases

PROiO74965.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR011387. TIF2A.
IPR013979. TIF_beta_prop-like.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamiPF08662. eIF2A. 1 hit.
[Graphical view]
PIRSFiPIRSF017222. eIF2A. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiEIF2A_SCHPO
AccessioniPrimary (citable) accession number: O74965
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: November 1, 1998
Last modified: September 7, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.