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Protein

Eukaryotic translation initiation factor 2A

Gene

SPBC4B4.04

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis, Translation regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 2A
Short name:
eIF-2A
Gene namesi
ORF Names:SPBC4B4.04
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC4B4.04.
PomBaseiSPBC4B4.04.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002860831 – 576Eukaryotic translation initiation factor 2AAdd BLAST576

Proteomic databases

MaxQBiO74965.
PRIDEiO74965.

Interactioni

Protein-protein interaction databases

BioGridi277515. 174 interactors.
MINTiMINT-4682114.

Structurei

Secondary structure

1576
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 13Combined sources8
Beta strandi15 – 19Combined sources5
Helixi20 – 22Combined sources3
Beta strandi37 – 42Combined sources6
Beta strandi46 – 55Combined sources10
Beta strandi57 – 61Combined sources5
Turni62 – 64Combined sources3
Beta strandi67 – 71Combined sources5
Beta strandi75 – 81Combined sources7
Beta strandi85 – 92Combined sources8
Beta strandi106 – 113Combined sources8
Beta strandi116 – 120Combined sources5
Beta strandi138 – 143Combined sources6
Beta strandi146 – 152Combined sources7
Beta strandi160 – 162Combined sources3
Beta strandi170 – 173Combined sources4
Beta strandi176 – 178Combined sources3
Beta strandi180 – 185Combined sources6
Beta strandi193 – 201Combined sources9
Beta strandi203 – 214Combined sources12
Beta strandi220 – 222Combined sources3
Beta strandi228 – 233Combined sources6
Beta strandi242 – 247Combined sources6
Beta strandi267 – 269Combined sources3
Beta strandi271 – 276Combined sources6
Beta strandi283 – 297Combined sources15
Beta strandi299 – 301Combined sources3
Beta strandi303 – 311Combined sources9
Beta strandi314 – 317Combined sources4
Beta strandi321 – 327Combined sources7
Beta strandi330 – 333Combined sources4
Beta strandi335 – 340Combined sources6
Turni341 – 344Combined sources4
Beta strandi345 – 352Combined sources8
Beta strandi358 – 361Combined sources4
Beta strandi365 – 372Combined sources8
Turni374 – 376Combined sources3
Beta strandi382 – 390Combined sources9
Beta strandi392 – 397Combined sources6
Beta strandi399 – 406Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WJ9X-ray2.51A/B2-412[»]
ProteinModelPortaliO74965.
SMRiO74965.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati71 – 119WD 1Add BLAST49
Repeati266 – 307WD 2Add BLAST42
Repeati308 – 349WD 3Add BLAST42
Repeati351 – 396WD 4Add BLAST46

Sequence similaritiesi

Belongs to the WD repeat EIF2A family.Curated
Contains 4 WD repeats.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

HOGENOMiHOG000193775.
InParanoidiO74965.
KOiK15026.
OMAiCARVVTN.
OrthoDBiEOG092C2TXG.
PhylomeDBiO74965.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR011387. TIF2A.
IPR013979. TIF_beta_prop-like.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamiPF08662. eIF2A. 1 hit.
[Graphical view]
PIRSFiPIRSF017222. eIF2A. 1 hit.

Sequencei

Sequence statusi: Complete.

O74965-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQKSQFAYR SSKSIGLVNA SENYASPPKF EAISEPARNA CYSPNGKLFA
60 70 80 90 100
YATATQVVIN DTESGAKLTQ LPAANTYELG FSPLGKYLST WERPGKEADG
110 120 130 140 150
TPKQNMKVWN TETGQLVFSF VQRNQTGWNL QYTCDESLAA RLVTNEVHFY
160 170 180 190 200
ETGNMSKGPI AKLRVEGISD FALSPGQNHA VAVFIPEKKG APASVRTYSI
210 220 230 240 250
PNFNSPLSQK TFFKADKVQF KWNALGTSLL VLTQTEVDKS NKNYYGETNL
260 270 280 290 300
YLLGITGQFD CRVDLDREGP IHDVCWNADS KEFGIVYGYM PAKTAIFDNR
310 320 330 340 350
ANVVSIIPPA PRNTLIFSPN SRYILLAGFG NLQGSIDIFD AANNMKKITT
360 370 380 390 400
VEAANCTYCE FSPDSQFLLT AVTSPRLRVD NSIKIWHITG APMFYEEFNE
410 420 430 440 450
LYQAFWRPRP LNPTLLQNAL TSASLPSPPT PHASASKLAA KPSVKPAGAY
460 470 480 490 500
RPPGARGQNS TFSYRREEID VMSSGSANKH VNSSRQRVVP GATPVIDGNK
510 520 530 540 550
KNNKKTKPAV KQVAQPTAEV SDEKKIRSLC KKLRAIDDLK SRLNNNEKLE
560 570
ATQVKKIESE GKVLAELKAL GWTPDA
Length:576
Mass (Da):63,497
Last modified:November 1, 1998 - v1
Checksum:i6702ADCCAF5A2A62
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA19284.1.
PIRiT40476.
RefSeqiNP_596421.1. NM_001022340.2.

Genome annotation databases

EnsemblFungiiSPBC4B4.04.1; SPBC4B4.04.1:pep; SPBC4B4.04.
GeneIDi2540999.
KEGGispo:SPBC4B4.04.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA19284.1.
PIRiT40476.
RefSeqiNP_596421.1. NM_001022340.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WJ9X-ray2.51A/B2-412[»]
ProteinModelPortaliO74965.
SMRiO74965.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277515. 174 interactors.
MINTiMINT-4682114.

Proteomic databases

MaxQBiO74965.
PRIDEiO74965.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC4B4.04.1; SPBC4B4.04.1:pep; SPBC4B4.04.
GeneIDi2540999.
KEGGispo:SPBC4B4.04.

Organism-specific databases

EuPathDBiFungiDB:SPBC4B4.04.
PomBaseiSPBC4B4.04.

Phylogenomic databases

HOGENOMiHOG000193775.
InParanoidiO74965.
KOiK15026.
OMAiCARVVTN.
OrthoDBiEOG092C2TXG.
PhylomeDBiO74965.

Miscellaneous databases

PROiO74965.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR011387. TIF2A.
IPR013979. TIF_beta_prop-like.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamiPF08662. eIF2A. 1 hit.
[Graphical view]
PIRSFiPIRSF017222. eIF2A. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiEIF2A_SCHPO
AccessioniPrimary (citable) accession number: O74965
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.