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Protein

Protein argonaute

Gene

ago1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for G1 arrest and mating in response to nitrogen starvation. Ago1 regulation of cytokinesis and cell cycle checkpoints occurs downstream of dcr1. Required, indirectly, for regulated hyperphosphorylation of cdc2. Has a role in the RNA interference (RNAi) pathway which is important for heterochromatin formation, accurate chromosome segregation, centromere cohesion and telomere function during mitosis and meiosis. Required for silencing at the centromeres and for initiation of transcriptionally silent heterochromatin at the mating type locus. Promotes histone H3K9 methylation necessary for centromere function. Required for recruitment of swi6 and cohesin to an ectopic dg repeat. A member of the RNA-induced transcriptional silencing (RITS) complex which is involved in the biosynthesis of dsRNA from primer siRNAs provided by the RNA-directed RNA polymerase (RDRC) complex. Has ribonuclease H-like cleavage (slicing) activity towards target messages complementary to siRNA and can direct site-specific cleavage of RNA substrates via siRNA. Slicing activity is required for both post-transcriptional and transcriptional gene silencing as well as for histone H3 'Lys-10' methylation spreading, conversion of double-stranded siRNA to single-stranded siRNA and siRNA-dependent association of ago1 with chromatin. A member of the argonaute siRNA chaperone (ARC) complex which is required for histone H3K9 methylation, heterochromatin assembly and siRNA generation. The ARC complex contains mostly double-stranded siRNA.10 Publications

GO - Molecular functioni

  • endoribonuclease activity, producing 5'-phosphomonoesters Source: PomBase
  • RNA binding Source: PomBase
  • single-stranded RNA binding Source: PomBase

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • chromatin silencing at centromere Source: PomBase
  • chromatin silencing by small RNA Source: PomBase
  • chromosome segregation Source: UniProtKB-KW
  • conversion of ds siRNA to ss siRNA involved in RNA interference Source: PomBase
  • co-transcriptional gene silencing by RNA interference machinery Source: PomBase
  • heterochromatin assembly involved in chromatin silencing by small RNA Source: PomBase
  • heterochromatin organization involved in chromatin silencing Source: PomBase
  • negative regulation of transcription from RNA polymerase II promoter Source: PomBase
  • production of siRNA involved in chromatin silencing by small RNA Source: PomBase
  • regulation of histone H3-K9 methylation Source: PomBase
  • RNA phosphodiester bond hydrolysis, endonucleolytic Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Chromosome partition, RNA-mediated gene silencing

Enzyme and pathway databases

ReactomeiR-SPO-426486. Small interfering RNA (siRNA) biogenesis.
R-SPO-426496. Post-transcriptional silencing by small RNAs.
R-SPO-5578749. Transcriptional regulation by small RNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein argonaute
Alternative name(s):
Cell cycle control protein ago1
Eukaryotic translation initiation factor 2C 2-like protein ago1
PAZ Piwi domain protein ago1
Protein slicer
RNA interference pathway protein ago1
Gene namesi
Name:ago1
Synonyms:csp9
ORF Names:SPCC736.11
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC736.11.
PomBaseiSPCC736.11. ago1.

Subcellular locationi

GO - Cellular componenti

  • ARC complex Source: PomBase
  • chromosome, telomeric region Source: UniProtKB-SubCell
  • cytosol Source: PomBase
  • mating-type region heterochromatin Source: PomBase
  • nuclear pericentric heterochromatin Source: PomBase
  • nuclear subtelomeric heterochromatin Source: PomBase
  • RITS complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Nucleus, Telomere

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi276F → A: Decreased association with siRNAs. Normal histone H3 'K-10' methylation. 1 Publication1
Mutagenesisi580D → A: Lower target RNA cleavage activity. Up-regulated centromeric transcripts from the dg and dh heterochromatic repeats. Defective for centromeric heterochromatin assembly and silencing. Decreased association with siRNAs. 2 Publications1
Mutagenesisi651D → A: Lower target RNA cleavage activity. Up-regulated centromeric transcripts from the dg and dh heterochromatic repeats. 1 Publication1
Mutagenesisi788H → A: Up-regulated centromeric transcripts from the dg and dh heterochromatic repeats. Reduced siRNA corresponding to heterochromatic repeats. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001940651 – 834Protein argonauteAdd BLAST834

Proteomic databases

MaxQBiO74957.
PRIDEiO74957.

Interactioni

Subunit structurei

Ago1, chp1 and tas3 interact to form the core of the RNA-induced transcriptional silencing (RITS) complex. The RITS complex interacts with the RDRC complex via interaction between ago1 and hrr1. Clr4 has a role in mediating this interaction. Component of the argonaute siRNA chaperone (ARC) complex composed of ago1, arb1 and arb2. Interacts with arb1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SPBP8B7.28cO942762EBI-422882,EBI-2651917

Protein-protein interaction databases

BioGridi275968. 213 interactors.
DIPiDIP-29298N.
IntActiO74957. 4 interactors.
MINTiMINT-4682059.

Structurei

3D structure databases

ProteinModelPortaliO74957.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini212 – 326PAZPROSITE-ProRule annotationAdd BLAST115
Domaini500 – 799PiwiPROSITE-ProRule annotationAdd BLAST300

Sequence similaritiesi

Belongs to the argonaute family. Ago subfamily.Curated
Contains 1 PAZ domain.PROSITE-ProRule annotation
Contains 1 Piwi domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000116043.
InParanoidiO74957.
KOiK11593.
OMAiMCHTYAR.
OrthoDBiEOG092C0NVF.
PhylomeDBiO74957.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR014811. ArgoL1.
IPR032472. ArgoL2.
IPR032473. Argonaute_Mid_dom.
IPR032474. Argonaute_N.
IPR003100. PAZ_dom.
IPR003165. Piwi.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF08699. ArgoL1. 1 hit.
PF16488. ArgoL2. 1 hit.
PF16487. ArgoMid. 1 hit.
PF16486. ArgoN. 1 hit.
PF02170. PAZ. 1 hit.
PF02171. Piwi. 1 hit.
[Graphical view]
SMARTiSM01163. DUF1785. 1 hit.
SM00949. PAZ. 1 hit.
SM00950. Piwi. 1 hit.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEiPS50821. PAZ. 1 hit.
PS50822. PIWI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O74957-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYKPSSEIA LRPGYGGLGK QITLKANFFQ IISLPNETIN QYHVIVGDGS
60 70 80 90 100
RVPRKQSQLI WNSKEVKQYF GSSWMNSVYD GRSMCWSKGD IADGTIKVNI
110 120 130 140 150
GSESHPREIE FSIQKSSKIN LHTLSQFVNS KYSSDPQVLS SIMFLDLLLK
160 170 180 190 200
KKPSETLFGF MHSFFTGENG VSLGGGVEAW KGFYQSIRPN QGFMSVNVDI
210 220 230 240 250
SSSAFWRNDS LLQILMEYTD CSNVRDLTRF DLKRLSRKFR FLKVTCQHRN
260 270 280 290 300
NVGTDLANRV YSIEGFSSKS ASDSFFVRRL NGEEQKISVA EYFLENHNVR
310 320 330 340 350
LQYPNLPCIL VKNGAMLPIE FCFVVKGQRY TAKLNSDQTA NMIRFAVQRP
360 370 380 390 400
FERVQQIDDF VHQMDWDTDP YLTQYGMKIQ KKMLEVPARV LETPSIRYGG
410 420 430 440 450
DCIERPVSGR WNLRGKRFLD PPRAPIRSWA VMCFTSTRRL PMRGIENFLQ
460 470 480 490 500
TYVQTLTSLG INFVMKKPPV LYADIRGSVE ELCITLYKKA EQVGNAPPDY
510 520 530 540 550
LFFILDKNSP EPYGSIKRVC NTMLGVPSQC AISKHILQSK PQYCANLGMK
560 570 580 590 600
INVKVGGINC SLIPKSNPLG NVPTLILGGD VYHPGVGATG VSIASIVASV
610 620 630 640 650
DLNGCKYTAV SRSQPRHQEV IEGMKDIVVY LLQGFRAMTK QQPQRIIYFR
660 670 680 690 700
DGTSEGQFLS VINDELSQIK EACHSLSPKY NPKILVCTTQ KRHHARFFIK
710 720 730 740 750
NKSDGDRNGN PLPGTIIEKH VTHPYQYDFY LISHPSLQGV SVPVHYTVLH
760 770 780 790 800
DEIQMPPDQF QTLCYNLCYV YARATSAVSL VPPVYYAHLV SNLARYQDVT
810 820 830
ADDTFVETSE ASMDQEVKPL LALSSKLKTK MWYM
Length:834
Mass (Da):94,439
Last modified:November 1, 1998 - v1
Checksum:iF5CE397BF590D9E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA19275.1.
PIRiT41568.
RefSeqiNP_587782.1. NM_001022775.2.

Genome annotation databases

EnsemblFungiiSPCC736.11.1; SPCC736.11.1:pep; SPCC736.11.
GeneIDi2539403.
KEGGispo:SPCC736.11.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA19275.1.
PIRiT41568.
RefSeqiNP_587782.1. NM_001022775.2.

3D structure databases

ProteinModelPortaliO74957.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275968. 213 interactors.
DIPiDIP-29298N.
IntActiO74957. 4 interactors.
MINTiMINT-4682059.

Proteomic databases

MaxQBiO74957.
PRIDEiO74957.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC736.11.1; SPCC736.11.1:pep; SPCC736.11.
GeneIDi2539403.
KEGGispo:SPCC736.11.

Organism-specific databases

EuPathDBiFungiDB:SPCC736.11.
PomBaseiSPCC736.11. ago1.

Phylogenomic databases

HOGENOMiHOG000116043.
InParanoidiO74957.
KOiK11593.
OMAiMCHTYAR.
OrthoDBiEOG092C0NVF.
PhylomeDBiO74957.

Enzyme and pathway databases

ReactomeiR-SPO-426486. Small interfering RNA (siRNA) biogenesis.
R-SPO-426496. Post-transcriptional silencing by small RNAs.
R-SPO-5578749. Transcriptional regulation by small RNAs.

Miscellaneous databases

PROiO74957.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR014811. ArgoL1.
IPR032472. ArgoL2.
IPR032473. Argonaute_Mid_dom.
IPR032474. Argonaute_N.
IPR003100. PAZ_dom.
IPR003165. Piwi.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF08699. ArgoL1. 1 hit.
PF16488. ArgoL2. 1 hit.
PF16487. ArgoMid. 1 hit.
PF16486. ArgoN. 1 hit.
PF02170. PAZ. 1 hit.
PF02171. Piwi. 1 hit.
[Graphical view]
SMARTiSM01163. DUF1785. 1 hit.
SM00949. PAZ. 1 hit.
SM00950. Piwi. 1 hit.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEiPS50821. PAZ. 1 hit.
PS50822. PIWI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAGO1_SCHPO
AccessioniPrimary (citable) accession number: O74957
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1998
Last modified: October 5, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.