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Protein

Ribosome assembly protein 1

Gene

ria1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

GTPase involved in the biogenesis of the 60S ribosomal subunit and translational activation of ribosomes. Together with sdo1, may trigger the GTP-dependent release of tif6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating tif6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Inhibits GTPase activity of ribosome-bound EF-2 (By similarity).By similarity

Catalytic activityi

GTP + H2O = GDP + phosphate.

Enzyme regulationi

GTPase activity is stimulated in the presence of 60S subunits.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 338GTPBy similarity
Nucleotide bindingi100 – 1045GTPBy similarity
Nucleotide bindingi154 – 1574GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ribosome biogenesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome assembly protein 1 (EC:3.6.5.-)
Alternative name(s):
EF-2 like GTPase
Elongation factor-like 1
Gene namesi
Name:ria1
ORF Names:SPCC553.08c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC553.08c.
PomBaseiSPCC553.08c. ria1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10001000Ribosome assembly protein 1PRO_0000315632Add
BLAST

Proteomic databases

MaxQBiO74945.

Interactioni

Protein-protein interaction databases

BioGridi275861. 29 interactions.
MINTiMINT-4681987.

Structurei

3D structure databases

ProteinModelPortaliO74945.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 246230tr-type GPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family.PROSITE-ProRule annotation
Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000231586.
InParanoidiO74945.
KOiK14536.
OMAiGHLERIW.
OrthoDBiEOG7PVWXS.
PhylomeDBiO74945.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O74945-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVIPPEKLV SLQKNQENIR NFTLLAHVDH GKTTLADSLL ASNGIISSKL
60 70 80 90 100
AGTVRFLDFR EDEITRGITM KSSAISLFFK VISQNDEKRV EKDYLINLID
110 120 130 140 150
SPGHVDFSSE VSSASRLCDG AFVLVDAVEG VCSQTITVLR QAWIDRIKVI
160 170 180 190 200
LVINKMDRLI TELKLSPIEA HYHLLRLVEQ VNAVIGTFYT GELMQLADND
210 220 230 240 250
EVISDEGIYF APEQGNVVFA SAYDGWAFCL DQFSEFYEKK LGLKQKALTK
260 270 280 290 300
CLWGDYYLDP KTKRVLQPKH LQGRRLKPMF VQFVLENLWA VYESAVSNRN
310 320 330 340 350
LENIEKIIKA LNIKVLPRDI KSKDPRNLLL AIFQQWLPLS TAILLTAIRE
360 370 380 390 400
IPSPINAQAN RARKVLSSTP HYEMIDPDIT LAMESCDASK EQPVLVYISK
410 420 430 440 450
MVAFSERDLP NHRRKQLSAE EMKLIRSKLS ESIESGINTI SIEENVSSTN
460 470 480 490 500
SDNLEGSTTD MDDDKDILIG FARIYSGTIS VGQEVYVYGP KYDPVNPEKH
510 520 530 540 550
ITKVTVESLY LMMGQELVYL ETVPAGNVFA IGGLAGTVLR TATLCSSPNG
560 570 580 590 600
PNLVGVTQQM EPIVRVALEP VRPFEMNKLV TGLDMLNQAD PCVQIAVEEN
610 620 630 640 650
GEHVIMCAGE IHLERCLKDL RERFAKIEIQ ASQPLVPYRE TTIATPDLLA
660 670 680 690 700
KNKELSIGFV TATLPVGGVT IGITVTPLSG SVVDFLLKHS KTIENVSSNF
710 720 730 740 750
SKKNRNVVVS ESLTKSMEEV LTPEKFYERL SKLLEEENSD LGELKNHLDS
760 770 780 790 800
IIAFGPKRVG PNILFDKTKK MRDFRRQSDE TKLIPSDLSE YVVTAFQLIT
810 820 830 840 850
HQGPLCAEPV QGICVSIDQF DISDDSEDSK LLTINNPQIP GQVISVVKES
860 870 880 890 900
IRHGFLGWSP RLMLAMYSCD VQATSEVLGR VYGVVSKRRG RVIDEEMKEG
910 920 930 940 950
TPFFIVKALI PVVESFGFAV EILKRTSGAA YPQLIFHGFE MLDENPFWVP
960 970 980 990 1000
TTEEELEDLG ELADRENIAK RYMLNVRKRK GLLVEQKIVE KAEKQRTLKH
Length:1,000
Mass (Da):112,129
Last modified:November 1, 1998 - v1
Checksum:iE0B3624D52560702
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA19260.1.
PIRiT41396.
RefSeqiNP_587766.1. NM_001022759.2.

Genome annotation databases

EnsemblFungiiSPCC553.08c.1; SPCC553.08c.1:pep; SPCC553.08c.
GeneIDi2539293.
KEGGispo:SPCC553.08c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA19260.1.
PIRiT41396.
RefSeqiNP_587766.1. NM_001022759.2.

3D structure databases

ProteinModelPortaliO74945.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275861. 29 interactions.
MINTiMINT-4681987.

Proteomic databases

MaxQBiO74945.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC553.08c.1; SPCC553.08c.1:pep; SPCC553.08c.
GeneIDi2539293.
KEGGispo:SPCC553.08c.

Organism-specific databases

EuPathDBiFungiDB:SPCC553.08c.
PomBaseiSPCC553.08c. ria1.

Phylogenomic databases

HOGENOMiHOG000231586.
InParanoidiO74945.
KOiK14536.
OMAiGHLERIW.
OrthoDBiEOG7PVWXS.
PhylomeDBiO74945.

Miscellaneous databases

PROiO74945.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRIA1_SCHPO
AccessioniPrimary (citable) accession number: O74945
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 1, 1998
Last modified: June 8, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.