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Protein

DNA polymerase kappa

Gene

mug40

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Has a role in meiosis.2 Publications

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi136MagnesiumBy similarity1
Metal bindingi226MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: PomBase
  • DNA replication Source: UniProtKB-KW
  • error-free translesion synthesis Source: PomBase
  • meiotic cell cycle Source: UniProtKB-KW
  • response to intra-S DNA damage checkpoint signaling Source: PomBase
  • translesion synthesis Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication, DNA synthesis, Meiosis

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase kappa (EC:2.7.7.7)
Alternative name(s):
Meiotically up-regulated gene 40 protein
Gene namesi
Name:mug40
ORF Names:SPCC553.07c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC553.07c.
PomBaseiSPCC553.07c.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • nuclear chromatin Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002785221 – 547DNA polymerase kappaAdd BLAST547

Proteomic databases

MaxQBiO74944.
PRIDEiO74944.

Interactioni

Subunit structurei

Interacts with hus1 and rad17.1 Publication

Protein-protein interaction databases

BioGridi276108. 9 interactors.
MINTiMINT-4681974.

Structurei

3D structure databases

ProteinModelPortaliO74944.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini132 – 316UmuCAdd BLAST185

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi25 – 28Poly-Ser4

Sequence similaritiesi

Contains 1 umuC domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

InParanoidiO74944.
KOiK03511.
OMAiKYGCRSG.
OrthoDBiEOG092C4SHK.
PhylomeDBiO74944.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
HAMAPiMF_01113. DNApol_IV. 1 hit.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR022880. DNApol_IV.
IPR001126. UmuC.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O74944-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENAKDFIGE TIKENGLLTI EDDGSSSSDE EATLKRRLAG PSVLKSGQEN
60 70 80 90 100
VNQKKINEII YEASKGSKFF EAEQKRDREL RLRIEKVQVE VEKYQSKLRF
110 120 130 140 150
DKAFQREWTI RQESVDTTVE DFRAKRDLTQ IIVHVDCDAF YASIEELKNP
160 170 180 190 200
KLKSLPMAVG KSVLCTANYV ARKFGVRSAM PEFIARKICP DLVVIPLNLS
210 220 230 240 250
EYAIKSKEIQ NVLAQYDSNL CPASIDEFYM NLTSHLRLQE LAFTVENITM
260 270 280 290 300
VVEKIRKQVH EETGVTVSCG IAANKLLAKI ASNKRKPNNQ FFIPFDEIGI
310 320 330 340 350
SKFMNDLPVR EVSGIGRVLE QQLLGLEIKT CGDIQRNLVI LSYIFLPKSF
360 370 380 390 400
QNLLRCSYGF GTTILDEYGE SKRKTIGSEA TFSSNLSSPS IIEYKLRLLV
410 420 430 440 450
QNVSENLQKR GLVTNSIAIK YKTSEFQVHT KQKSIGQFIH SESDLLKPAL
460 470 480 490 500
QLLRQSYPMT IRLLGVRATK LVSKSRCLAM QLKFQSQNTV PCPVCQKNIE
510 520 530 540
NELGILNQHV DLCLNVETVK SLINTDHTAN PTIKKRKSNT LDTYFLE
Length:547
Mass (Da):62,055
Last modified:October 11, 2004 - v3
Checksum:iC18B08BF11336C71
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA19259.2.
PIRiT41397.
RefSeqiNP_587767.2. NM_001022760.2.

Genome annotation databases

EnsemblFungiiSPCC553.07c.1; SPCC553.07c.1:pep; SPCC553.07c.
GeneIDi2539547.
KEGGispo:SPCC553.07c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA19259.2.
PIRiT41397.
RefSeqiNP_587767.2. NM_001022760.2.

3D structure databases

ProteinModelPortaliO74944.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276108. 9 interactors.
MINTiMINT-4681974.

Proteomic databases

MaxQBiO74944.
PRIDEiO74944.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC553.07c.1; SPCC553.07c.1:pep; SPCC553.07c.
GeneIDi2539547.
KEGGispo:SPCC553.07c.

Organism-specific databases

EuPathDBiFungiDB:SPCC553.07c.
PomBaseiSPCC553.07c.

Phylogenomic databases

InParanoidiO74944.
KOiK03511.
OMAiKYGCRSG.
OrthoDBiEOG092C4SHK.
PhylomeDBiO74944.

Miscellaneous databases

PROiO74944.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
HAMAPiMF_01113. DNApol_IV. 1 hit.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR022880. DNApol_IV.
IPR001126. UmuC.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPOLK_SCHPO
AccessioniPrimary (citable) accession number: O74944
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: October 11, 2004
Last modified: October 5, 2016
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.