O74928 (PUR9_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional purine biosynthesis protein ade10

Including the following 2 domains:

1. Phosphoribosylaminoimidazolecarboxamide formyltransferase
   EC=2.1.2.3
   Alternative name(s):
   5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase
   AICAR transformylase
2. IMP cyclohydrolase
   EC=3.5.4.10
   Alternative name(s):
   ATIC
   IMP synthase
   Inosinicase

Gene names

Name:	ade10
ORF Names:	SPCPB16A4.03c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Subunit structure	Homodimer By similarity.
Domain	The IMP cyclohydrolase activity resides in the N-terminal region By similarity.
Sequence similarities	Belongs to the PurH family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Hydrolase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway IMP biosynthetic process Inferred from mutant phenotype Ref.1. Source: PomBase
Cellular_component	cytosol Inferred from direct assay PubMed 16823372. Source: PomBase
Molecular_function	IMP cyclohydrolase activity Inferred from mutant phenotype Ref.1. Source: PomBase phosphoribosylaminoimidazolecarboxamide formyltransferase activity Inferred from mutant phenotype Ref.1. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 585	585	Bifunctional purine biosynthesis protein ade10		PRO_0000192158
Experimental info
	Sequence conflict	404	1	K → Q in BAA13904. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	O74928 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 103031D3F957529F	FASTA	585	64,123
	10 20 30 40 50 60 MYALLSVYDK TGLLELAKAL TSKGVKLLGS GGTAKMIRES GMEVADVSSI TNAPEILGGR 70 80 90 100 110 120 VKTLHPAVHG GILARDIPSD EKDLVEQSIE KIDIVVCNLY PFRETIAKPN VTIPEAVEEI 130 140 150 160 170 180 DIGGVTLLRA AAKNHARVTI LSDPADYATF TDKFLSDKLT QDDRNTYALK AFASTASYDA 190 200 210 220 230 240 AITDYFRKQY AAGVDQLTLR YGANPHQSPA QAFMEQGPLP FKVLCGSPGY INLMDALNSW 250 260 270 280 290 300 PLVKELRENI GIPAAASFKH VSPAGAAVGL PLSDVEKKVY FVSDITEFTP LACAYARARG 310 320 330 340 350 360 ADRMSSFGDF IALSDTVDVC TARIISREVS DGVIAPGYEP EALELLKKKK GGKYCVLQMD 370 380 390 400 410 420 PKYVPAEIET RQVYGISLQQ HRNHAKIDFS LFEKVVSKNK DLPKSALIDL VIATTALKYT 430 440 450 460 470 480 QSNSVCYAKN GMVVGLGAGQ QSRIHCNRLA GDKADNWWLR HHPKVLGMQF KKSAKRPEKS 490 500 510 520 530 540 NAIDLYVLDA VPAEGSEREQ WESAFETIPE PLTKKEREEF LATCKDVVCA SDAFFPFPDN 550 560 570 580 IYRLAQSGVK YVAAPGGSVM DQAVRDTANE FNMVFSEIPL RLFHH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and sequence analysis of the Schizosaccharomyces pombe ade10+ gene." Liedtke C., Schmidt H. Yeast 14:1307-1310(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[3]	"Identification of open reading frames in Schizosaccharomyces pombe cDNAs." Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H. DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 166-585. Strain: PR745.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y16419 Genomic DNA. Translation: CAA76207.1. CU329672 Genomic DNA. Translation: CAC39322.1. D89243 mRNA. Translation: BAA13904.1.
PIR	T43150.
RefSeq	NP_588027.1. NM_001023018.2.
3D structure databases
ProteinModelPortal	O74928.
SMR	O74928. Positions 3-585.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPCPB16A4.03c-1.
Proteomic databases
PaxDb	O74928.
PRIDE	O74928.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPCPB16A4.03c.1; SPCPB16A4.03c.1:pep; SPCPB16A4.03c.
GeneID	2539569.
KEGG	spo:SPCPB16A4.03c.
Organism-specific databases
PomBase	SPCPB16A4.03c.
Phylogenomic databases
eggNOG	COG0138.
HOGENOM	HOG000230372.
KO	K00602.
OMA	DLLFAWK.
OrthoDB	EOG4D82DW.
Enzyme and pathway databases
UniPathway	UPA00074; UER00133. UPA00074; UER00135.
Family and domain databases
Gene3D	1.10.287.440. 1 hit. 3.40.140.20. 3 hits. 3.40.50.1380. 1 hit.
InterPro	IPR024051. AICAR_Tfase_dom. IPR024050. AICAR_Tfase_insert_dom. IPR002695. AICARFT_IMPCHas. IPR016193. Cytidine_deaminase-like. IPR011607. MGS-like_dom. [Graphical view]
PANTHER	PTHR11692. PTHR11692. 1 hit.
Pfam	PF01808. AICARFT_IMPCHas. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PIRSF	PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMART	SM00798. AICARFT_IMPCHas. 1 hit. SM00851. MGS. 1 hit. [Graphical view]
SUPFAM	SSF53927. Cytidine_deaminase-like. 1 hit. SSF52335. MGS-like_dom. 1 hit.
TIGRFAMs	TIGR00355. purH. 1 hit.
ProtoNet	Search...
Other
NextBio	20800728.

Entry information

Entry name

PUR9_SCHPO

Accession

Primary (citable) accession number: O74928
Secondary accession number(s): P78892

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 14, 2001
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program