ID   MTHR2_SCHPO             Reviewed;         641 AA.
AC   O74927; P78770; Q9UT80;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   14-AUG-2001, sequence version 2.
DT   16-JUN-2009, entry version 58.
DE   RecName: Full=Methylenetetrahydrofolate reductase 2;
DE            EC=1.5.1.20;
GN   Name=met11; Synonyms=mthfr2; ORFNames=SPAC343.10;
OS   Schizosaccharomyces pombe (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales;
OC   Schizosaccharomycetaceae; Schizosaccharomyces.
OX   NCBI_TaxID=4896;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 38364 / 968;
RA   Naula N.;
RL   Thesis (1998), University of Bern, Switzerland.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 38366 / 972;
RX   MEDLINE=21848401; PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M.,
RA   Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A.,
RA   Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G.,
RA   Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K.,
RA   James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J.,
RA   Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C.,
RA   Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E.,
RA   Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S.,
RA   Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K.,
RA   Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S.,
RA   Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B.,
RA   Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S.,
RA   Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D.,
RA   Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R.,
RA   Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B.,
RA   Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S.,
RA   Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M.,
RA   Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G.,
RA   Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J.,
RA   Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L.,
RA   Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J.,
RA   Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 11-641.
RC   STRAIN=PR745;
RX   MEDLINE=98162722; PubMed=9501991; DOI=10.1093/dnares/4.6.363;
RA   Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.;
RT   "Identification of open reading frames in Schizosaccharomyces pombe
RT   cDNAs.";
RL   DNA Res. 4:363-369(1997).
CC   -!- CATALYTIC ACTIVITY: 5-methyltetrahydrofolate + NAD(P)(+) = 5,10-
CC       methylenetetrahydrofolate + NAD(P)H.
CC   -!- COFACTOR: FAD (By similarity).
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate pathway.
CC   -!- SIMILARITY: Belongs to the methylenetetrahydrofolate reductase
CC       family.
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DR   EMBL; AJ011686; CAA09738.1; -; Genomic_DNA.
DR   EMBL; CU329670; CAB52273.1; -; Genomic_DNA.
DR   EMBL; D89118; BAA13780.1; -; mRNA.
DR   PIR; T38659; T38659.
DR   RefSeq; NP_593430.1; -.
DR   HSSP; P00394; 1B5T.
DR   GeneID; 2543105; -.
DR   KEGG; spo:SPAC343.10; -.
DR   NMPDR; fig|4896.1.peg.3400; -.
DR   GeneDB_Spombe; SPAC343.10; -.
DR   OMA; O74927; YGEIDGY.
DR   BioCyc; SPOM-XXX-01:SPOM-XXX-01-001116-MON; -.
DR   BRENDA; 1.5.1.20; 653.
DR   ArrayExpress; O74927; -.
DR   GO; GO:0004489; F:methylenetetrahydrofolate reductase (NADPH)...; IDA:GeneDB_SPombe.
DR   GO; GO:0006555; P:methionine metabolic process; IDA:GeneDB_SPombe.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR004621; Fadh2_euk.
DR   InterPro; IPR003171; Mehydrof_redctse.
DR   Pfam; PF02219; MTHFR; 1.
DR   TIGRFAMs; TIGR00677; fadh2_euk; 1.
PE   2: Evidence at transcript level;
KW   Complete proteome; FAD; Flavoprotein; NADP; Oxidoreductase.
FT   CHAIN         1    641       Methylenetetrahydrofolate reductase 2.
FT                                /FTId=PRO_0000190254.
FT   CONFLICT    202    202       E -> D (in Ref. 3; BAA13780).
FT   CONFLICT    281    281       G -> A (in Ref. 3; BAA13780).
FT   CONFLICT    455    455       A -> V (in Ref. 3; BAA13780).
FT   CONFLICT    537    641       QVTYYAGNNKSEFLTNAPKDGASAVTWGVYPGREIIQSTII
FT                                AEVSFKAWLSESFQVWGEWANLYSKNTPSRKLLENCINDRW
FT                                LVTVIHHDFMDKNGLWKVLEIDF -> HVRSNFSSSISDST
FT                                CFQRLVSKPLFLRIRATDRSKFSVQK (in Ref. 3;
FT                                BAA13780).
FT   CONFLICT    614    614       I -> L (in Ref. 1; CAA09738).
SQ   SEQUENCE   641 AA;  72140 MW;  E7AFFDD1F2C1C8A4 CRC64;
     MKIVDLIDKI PNGNAFYSFE YFPPKTTVGL ENLSSRITRM SRNMMPLFSS VTWGTAGSTA
     EVSIYLAKML EQHHKIPACL HLTCTNVDKA IIDKALETAK EYGIRNILAL RGDPPLNSDH
     WEGKVSEFEH AVDLVRYIRE KYGDYFCIGV AAYPEGHVDS NVPELSKDPL RDIPFLIEKV
     EAGADFIITQ IFYEPEAFIK FENFVRNHSS NALRNIPIIP AIMPIQSYGS LKRMTRLCGC
     SVPSSLMQRL NAAKPDDEAI KNIGVEHIVD MIKKIMDNVQ GRVHGFHFCT LNLERSVALI
     LKNSGLLTKR WKQVESEMED EKLLRTTRKR LSLDEPAELH NQVVVPSQQP VADKSSNLFV
     TSKQSSVSGH KDNLTEEAPF SVSEGSGVLG RQANWDDFTN GRFGDPRSPA YGEIDGYGPT
     LHFPPSEALK LWGYPVDESD ITSLFQKHIM SDISAIPWID EPVEVETKTI AKYLLKLNGN
     SWWTVGSQPA VNGAPSADPV FGWGPKGGRV FQKAFVECFV NGKDLKDFIT KWHDNPQVTY
     YAGNNKSEFL TNAPKDGASA VTWGVYPGRE IIQSTIIAEV SFKAWLSESF QVWGEWANLY
     SKNTPSRKLL ENCINDRWLV TVIHHDFMDK NGLWKVLEID F
//