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Protein

Methylenetetrahydrofolate reductase 2

Gene

met11

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Major methylenetetrahydrofolate reductase required to generate the methyl groups necessary for methionine synthetase to convert homocysteine to methionine. Performs 15 to 20 percent of the total methylenetetrahydrofolate reductase activity of the cells.

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.1 Publication

Cofactori

FADBy similarity

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei20Proton donor/acceptorBy similarity1
Binding sitei81FADBy similarity1
Binding sitei113SubstrateBy similarity1
Binding sitei153FADBy similarity1
Binding sitei172FADBy similarity1
Binding sitei179FADBy similarity1
Binding sitei190SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi20 – 25NADBy similarity6
Nucleotide bindingi52 – 53NAD and FADBy similarity2
Nucleotide bindingi111 – 113FADBy similarity3

GO - Molecular functioni

  • methylenetetrahydrofolate reductase (NAD(P)H) activity Source: PomBase

GO - Biological processi

  • methionine biosynthetic process Source: PomBase
  • one-carbon metabolic process Source: GO_Central
  • tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

ReactomeiR-SPO-196757. Metabolism of folate and pterines.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate reductase 2 (EC:1.5.1.20)
Gene namesi
Name:met11
Synonyms:mthfr2
ORF Names:SPAC343.10
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC343.10.
PomBaseiSPAC343.10. met11.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Leads to partial auxotrophy for methionine.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001902541 – 641Methylenetetrahydrofolate reductase 2Add BLAST641

Proteomic databases

MaxQBiO74927.
PRIDEiO74927.

Interactioni

Protein-protein interaction databases

BioGridi279537. 8 interactors.

Structurei

3D structure databases

ProteinModelPortaliO74927.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000246234.
InParanoidiO74927.
KOiK00297.
OMAiNVTWGAG.
OrthoDBiEOG092C12V2.
PhylomeDBiO74927.

Family and domain databases

CDDicd00537. MTHFR. 1 hit.
Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

O74927-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIVDLIDKI PNGNAFYSFE YFPPKTTVGL ENLSSRITRM SRNMMPLFSS
60 70 80 90 100
VTWGTAGSTA EVSIYLAKML EQHHKIPACL HLTCTNVDKA IIDKALETAK
110 120 130 140 150
EYGIRNILAL RGDPPLNSDH WEGKVSEFEH AVDLVRYIRE KYGDYFCIGV
160 170 180 190 200
AAYPEGHVDS NVPELSKDPL RDIPFLIEKV EAGADFIITQ IFYEPEAFIK
210 220 230 240 250
FENFVRNHSS NALRNIPIIP AIMPIQSYGS LKRMTRLCGC SVPSSLMQRL
260 270 280 290 300
NAAKPDDEAI KNIGVEHIVD MIKKIMDNVQ GRVHGFHFCT LNLERSVALI
310 320 330 340 350
LKNSGLLTKR WKQVESEMED EKLLRTTRKR LSLDEPAELH NQVVVPSQQP
360 370 380 390 400
VADKSSNLFV TSKQSSVSGH KDNLTEEAPF SVSEGSGVLG RQANWDDFTN
410 420 430 440 450
GRFGDPRSPA YGEIDGYGPT LHFPPSEALK LWGYPVDESD ITSLFQKHIM
460 470 480 490 500
SDISAIPWID EPVEVETKTI AKYLLKLNGN SWWTVGSQPA VNGAPSADPV
510 520 530 540 550
FGWGPKGGRV FQKAFVECFV NGKDLKDFIT KWHDNPQVTY YAGNNKSEFL
560 570 580 590 600
TNAPKDGASA VTWGVYPGRE IIQSTIIAEV SFKAWLSESF QVWGEWANLY
610 620 630 640
SKNTPSRKLL ENCINDRWLV TVIHHDFMDK NGLWKVLEID F
Length:641
Mass (Da):72,140
Last modified:August 14, 2001 - v2
Checksum:iE7AFFDD1F2C1C8A4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti202E → D in BAA13780 (PubMed:9501991).Curated1
Sequence conflicti281G → A in BAA13780 (PubMed:9501991).Curated1
Sequence conflicti455A → V in BAA13780 (PubMed:9501991).Curated1
Sequence conflicti537 – 641QVTYY…LEIDF → HVRSNFSSSISDSTCFQRLV SKPLFLRIRATDRSKFSVQK in BAA13780 (PubMed:9501991).CuratedAdd BLAST105
Sequence conflicti614I → L in CAA09738 (PubMed:12112238).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ011686 Genomic DNA. Translation: CAA09738.1.
CU329670 Genomic DNA. Translation: CAB52273.1.
D89118 mRNA. Translation: BAA13780.1.
PIRiT38659.
RefSeqiNP_593430.1. NM_001018863.2.

Genome annotation databases

EnsemblFungiiSPAC343.10.1; SPAC343.10.1:pep; SPAC343.10.
GeneIDi2543105.
KEGGispo:SPAC343.10.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ011686 Genomic DNA. Translation: CAA09738.1.
CU329670 Genomic DNA. Translation: CAB52273.1.
D89118 mRNA. Translation: BAA13780.1.
PIRiT38659.
RefSeqiNP_593430.1. NM_001018863.2.

3D structure databases

ProteinModelPortaliO74927.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279537. 8 interactors.

Proteomic databases

MaxQBiO74927.
PRIDEiO74927.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC343.10.1; SPAC343.10.1:pep; SPAC343.10.
GeneIDi2543105.
KEGGispo:SPAC343.10.

Organism-specific databases

EuPathDBiFungiDB:SPAC343.10.
PomBaseiSPAC343.10. met11.

Phylogenomic databases

HOGENOMiHOG000246234.
InParanoidiO74927.
KOiK00297.
OMAiNVTWGAG.
OrthoDBiEOG092C12V2.
PhylomeDBiO74927.

Enzyme and pathway databases

UniPathwayiUPA00193.
ReactomeiR-SPO-196757. Metabolism of folate and pterines.

Miscellaneous databases

PROiO74927.

Family and domain databases

CDDicd00537. MTHFR. 1 hit.
Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMTHR2_SCHPO
AccessioniPrimary (citable) accession number: O74927
Secondary accession number(s): P78770, Q9UT80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 14, 2001
Last modified: November 30, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.