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O74927

- MTHR2_SCHPO

UniProt

O74927 - MTHR2_SCHPO

Protein

Methylenetetrahydrofolate reductase 2

Gene

met11

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (14 Aug 2001)
      Previous versions | rss
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    Functioni

    Major methylenetetrahydrofolate reductase required to generate the methyl groups necessary for methionine synthetase to convert homocysteine to methionine. Performs 15 to 20 percent of the total methylenetetrahydrofolate reductase activity of the cells.

    Catalytic activityi

    5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.1 Publication

    Cofactori

    FAD.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei20 – 201Proton donor/acceptorBy similarity
    Binding sitei81 – 811FADBy similarity
    Binding sitei113 – 1131SubstrateBy similarity
    Binding sitei153 – 1531FADBy similarity
    Binding sitei172 – 1721FADBy similarity
    Binding sitei179 – 1791FADBy similarity
    Binding sitei190 – 1901SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi20 – 256NADBy similarity
    Nucleotide bindingi52 – 532NAD and FADBy similarity
    Nucleotide bindingi111 – 1133FADBy similarity

    GO - Molecular functioni

    1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: PomBase

    GO - Biological processi

    1. methionine biosynthetic process Source: PomBase
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein, NADP

    Enzyme and pathway databases

    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methylenetetrahydrofolate reductase 2 (EC:1.5.1.20)
    Gene namesi
    Name:met11
    Synonyms:mthfr2
    ORF Names:SPAC343.10
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC343.10.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: PomBase

    Pathology & Biotechi

    Disruption phenotypei

    Leads to partial auxotrophy for methionine.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 641641Methylenetetrahydrofolate reductase 2PRO_0000190254Add
    BLAST

    Proteomic databases

    MaxQBiO74927.

    Interactioni

    Protein-protein interaction databases

    BioGridi279537. 6 interactions.
    STRINGi4896.SPAC343.10-1.

    Structurei

    3D structure databases

    ProteinModelPortaliO74927.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0685.
    HOGENOMiHOG000246234.
    KOiK00297.
    OMAiNVTWGAG.
    OrthoDBiEOG7M0P15.
    PhylomeDBiO74927.

    Family and domain databases

    Gene3Di3.20.20.220. 1 hit.
    InterProiIPR029041. FAD-linked_oxidoreductase-like.
    IPR004621. Fadh2_euk.
    IPR003171. Mehydrof_redctse.
    [Graphical view]
    PfamiPF02219. MTHFR. 1 hit.
    [Graphical view]
    SUPFAMiSSF51730. SSF51730. 1 hit.
    TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O74927-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKIVDLIDKI PNGNAFYSFE YFPPKTTVGL ENLSSRITRM SRNMMPLFSS    50
    VTWGTAGSTA EVSIYLAKML EQHHKIPACL HLTCTNVDKA IIDKALETAK 100
    EYGIRNILAL RGDPPLNSDH WEGKVSEFEH AVDLVRYIRE KYGDYFCIGV 150
    AAYPEGHVDS NVPELSKDPL RDIPFLIEKV EAGADFIITQ IFYEPEAFIK 200
    FENFVRNHSS NALRNIPIIP AIMPIQSYGS LKRMTRLCGC SVPSSLMQRL 250
    NAAKPDDEAI KNIGVEHIVD MIKKIMDNVQ GRVHGFHFCT LNLERSVALI 300
    LKNSGLLTKR WKQVESEMED EKLLRTTRKR LSLDEPAELH NQVVVPSQQP 350
    VADKSSNLFV TSKQSSVSGH KDNLTEEAPF SVSEGSGVLG RQANWDDFTN 400
    GRFGDPRSPA YGEIDGYGPT LHFPPSEALK LWGYPVDESD ITSLFQKHIM 450
    SDISAIPWID EPVEVETKTI AKYLLKLNGN SWWTVGSQPA VNGAPSADPV 500
    FGWGPKGGRV FQKAFVECFV NGKDLKDFIT KWHDNPQVTY YAGNNKSEFL 550
    TNAPKDGASA VTWGVYPGRE IIQSTIIAEV SFKAWLSESF QVWGEWANLY 600
    SKNTPSRKLL ENCINDRWLV TVIHHDFMDK NGLWKVLEID F 641
    Length:641
    Mass (Da):72,140
    Last modified:August 14, 2001 - v2
    Checksum:iE7AFFDD1F2C1C8A4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti202 – 2021E → D in BAA13780. (PubMed:9501991)Curated
    Sequence conflicti281 – 2811G → A in BAA13780. (PubMed:9501991)Curated
    Sequence conflicti455 – 4551A → V in BAA13780. (PubMed:9501991)Curated
    Sequence conflicti537 – 641105QVTYY…LEIDF → HVRSNFSSSISDSTCFQRLV SKPLFLRIRATDRSKFSVQK in BAA13780. (PubMed:9501991)CuratedAdd
    BLAST
    Sequence conflicti614 – 6141I → L in CAA09738. (PubMed:12112238)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ011686 Genomic DNA. Translation: CAA09738.1.
    CU329670 Genomic DNA. Translation: CAB52273.1.
    D89118 mRNA. Translation: BAA13780.1.
    PIRiT38659.
    RefSeqiNP_593430.1. NM_001018863.2.

    Genome annotation databases

    EnsemblFungiiSPAC343.10.1; SPAC343.10.1:pep; SPAC343.10.
    GeneIDi2543105.
    KEGGispo:SPAC343.10.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ011686 Genomic DNA. Translation: CAA09738.1 .
    CU329670 Genomic DNA. Translation: CAB52273.1 .
    D89118 mRNA. Translation: BAA13780.1 .
    PIRi T38659.
    RefSeqi NP_593430.1. NM_001018863.2.

    3D structure databases

    ProteinModelPortali O74927.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 279537. 6 interactions.
    STRINGi 4896.SPAC343.10-1.

    Proteomic databases

    MaxQBi O74927.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC343.10.1 ; SPAC343.10.1:pep ; SPAC343.10 .
    GeneIDi 2543105.
    KEGGi spo:SPAC343.10.

    Organism-specific databases

    PomBasei SPAC343.10.

    Phylogenomic databases

    eggNOGi COG0685.
    HOGENOMi HOG000246234.
    KOi K00297.
    OMAi NVTWGAG.
    OrthoDBi EOG7M0P15.
    PhylomeDBi O74927.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .

    Miscellaneous databases

    NextBioi 20804132.

    Family and domain databases

    Gene3Di 3.20.20.220. 1 hit.
    InterProi IPR029041. FAD-linked_oxidoreductase-like.
    IPR004621. Fadh2_euk.
    IPR003171. Mehydrof_redctse.
    [Graphical view ]
    Pfami PF02219. MTHFR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51730. SSF51730. 1 hit.
    TIGRFAMsi TIGR00677. fadh2_euk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Two non-complementing genes encoding enzymatically active methylenetetrahydrofolate reductases control methionine requirement in fission yeast Schizosaccharomyces pombe."
      Naula N., Walther C., Baumann D., Schweingruber M.E.
      Yeast 19:841-848(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. "Identification of open reading frames in Schizosaccharomyces pombe cDNAs."
      Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.
      DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 11-641.
      Strain: PR745.

    Entry informationi

    Entry nameiMTHR2_SCHPO
    AccessioniPrimary (citable) accession number: O74927
    Secondary accession number(s): P78770, Q9UT80
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: August 14, 2001
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3