Reviewed, UniProtKB/Swiss-Prot O74927 (MTHR2_SCHPO)

Names and origin

Protein names

Recommended name:
    Methylenetetrahydrofolate reductase 2
    EC=1.5.1.20

Gene names

Name:	met11
Synonyms:	mthfr2
ORF Names:	SPAC343.10

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	641 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.
Cofactor	FAD By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate pathway.
Sequence similarities	Belongs to the methylenetetrahydrofolate reductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine metabolic process Inferred from direct assay. Source: GeneDB_SPombe oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	methylenetetrahydrofolate reductase (NADPH) activity Inferred from direct assay. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 641	641	Methylenetetrahydrofolate reductase 2		PRO_0000190254
Experimental info
	Sequence conflict	202	1	E → D in BAA13780. Ref.3
	Sequence conflict	281	1	G → A in BAA13780. Ref.3
	Sequence conflict	455	1	A → V in BAA13780. Ref.3
	Sequence conflict	537 – 641	105	QVTYY…LEIDF → HVRSNFSSSISDSTCFQRLV SKPLFLRIRATDRSKFSVQK in BAA13780. Ref.3
	Sequence conflict	614	1	I → L in CAA09738. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O74927-1 [UniParc]. Last modified August 14, 2001. Version 2. Checksum: E7AFFDD1F2C1C8A4	FASTA	641	72,140
	10 20 30 40 50 60 MKIVDLIDKI PNGNAFYSFE YFPPKTTVGL ENLSSRITRM SRNMMPLFSS VTWGTAGSTA 70 80 90 100 110 120 EVSIYLAKML EQHHKIPACL HLTCTNVDKA IIDKALETAK EYGIRNILAL RGDPPLNSDH 130 140 150 160 170 180 WEGKVSEFEH AVDLVRYIRE KYGDYFCIGV AAYPEGHVDS NVPELSKDPL RDIPFLIEKV 190 200 210 220 230 240 EAGADFIITQ IFYEPEAFIK FENFVRNHSS NALRNIPIIP AIMPIQSYGS LKRMTRLCGC 250 260 270 280 290 300 SVPSSLMQRL NAAKPDDEAI KNIGVEHIVD MIKKIMDNVQ GRVHGFHFCT LNLERSVALI 310 320 330 340 350 360 LKNSGLLTKR WKQVESEMED EKLLRTTRKR LSLDEPAELH NQVVVPSQQP VADKSSNLFV 370 380 390 400 410 420 TSKQSSVSGH KDNLTEEAPF SVSEGSGVLG RQANWDDFTN GRFGDPRSPA YGEIDGYGPT 430 440 450 460 470 480 LHFPPSEALK LWGYPVDESD ITSLFQKHIM SDISAIPWID EPVEVETKTI AKYLLKLNGN 490 500 510 520 530 540 SWWTVGSQPA VNGAPSADPV FGWGPKGGRV FQKAFVECFV NGKDLKDFIT KWHDNPQVTY 550 560 570 580 590 600 YAGNNKSEFL TNAPKDGASA VTWGVYPGRE IIQSTIIAEV SFKAWLSESF QVWGEWANLY 610 620 630 640 SKNTPSRKLL ENCINDRWLV TVIHHDFMDK NGLWKVLEID F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Naula N. Thesis (1998), University of Bern, Switzerland Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 38364 / 968.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[3]	"Identification of open reading frames in Schizosaccharomyces pombe cDNAs." Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H. DNA Res. 4:363-369(1997) [PubMed: 9501991] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 11-641. Strain: PR745.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ011686 Genomic DNA. Translation: CAA09738.1. CU329670 Genomic DNA. Translation: CAB52273.1. D89118 mRNA. Translation: BAA13780.1.
PIR	T38659.
RefSeq	NP_593430.1.
3D structure databases
HSSP	HSSP built from PDB template 1B5T based on UniProtKB P00394.
ModBase	Search...
Genome annotation databases
GeneID	2543105.
KEGG	spo:SPAC343.10.
NMPDR	fig|4896.1.peg.3400.
Organism-specific databases
GeneDB_Spombe	SPAC343.10.
Phylogenomic databases
OMA	O74927. YGEIDGY.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-001116-MON.
BRENDA	1.5.1.20. 653.
Gene expression databases
ArrayExpress	O74927.
Family and domain databases
InterPro	IPR004621. Fadh2_euk. IPR003171. Mehydrof_redctse. [Graphical view]
Pfam	PF02219. MTHFR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00677. fadh2_euk. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTHR2_SCHPO

Accession

Primary (citable) accession number: O74927
Secondary accession number(s): P78770, Q9UT80

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	August 14, 2001
Last modified:	June 16, 2009
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)