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O74927

- MTHR2_SCHPO

UniProt

O74927 - MTHR2_SCHPO

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Protein

Methylenetetrahydrofolate reductase 2

Gene

met11

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Major methylenetetrahydrofolate reductase required to generate the methyl groups necessary for methionine synthetase to convert homocysteine to methionine. Performs 15 to 20 percent of the total methylenetetrahydrofolate reductase activity of the cells.

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.1 Publication

Cofactori

FAD.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei20 – 201Proton donor/acceptorBy similarity
Binding sitei81 – 811FADBy similarity
Binding sitei113 – 1131SubstrateBy similarity
Binding sitei153 – 1531FADBy similarity
Binding sitei172 – 1721FADBy similarity
Binding sitei179 – 1791FADBy similarity
Binding sitei190 – 1901SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi20 – 256NADBy similarity
Nucleotide bindingi52 – 532NAD and FADBy similarity
Nucleotide bindingi111 – 1133FADBy similarity

GO - Molecular functioni

  1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: PomBase

GO - Biological processi

  1. methionine biosynthetic process Source: PomBase
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate reductase 2 (EC:1.5.1.20)
Gene namesi
Name:met11
Synonyms:mthfr2
ORF Names:SPAC343.10
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC343.10.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: PomBase
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Leads to partial auxotrophy for methionine.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 641641Methylenetetrahydrofolate reductase 2PRO_0000190254Add
BLAST

Proteomic databases

MaxQBiO74927.

Interactioni

Protein-protein interaction databases

BioGridi279537. 7 interactions.
STRINGi4896.SPAC343.10-1.

Structurei

3D structure databases

ProteinModelPortaliO74927.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0685.
HOGENOMiHOG000246234.
InParanoidiO74927.
KOiK00297.
OMAiNVTWGAG.
OrthoDBiEOG7M0P15.
PhylomeDBiO74927.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

O74927-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKIVDLIDKI PNGNAFYSFE YFPPKTTVGL ENLSSRITRM SRNMMPLFSS
60 70 80 90 100
VTWGTAGSTA EVSIYLAKML EQHHKIPACL HLTCTNVDKA IIDKALETAK
110 120 130 140 150
EYGIRNILAL RGDPPLNSDH WEGKVSEFEH AVDLVRYIRE KYGDYFCIGV
160 170 180 190 200
AAYPEGHVDS NVPELSKDPL RDIPFLIEKV EAGADFIITQ IFYEPEAFIK
210 220 230 240 250
FENFVRNHSS NALRNIPIIP AIMPIQSYGS LKRMTRLCGC SVPSSLMQRL
260 270 280 290 300
NAAKPDDEAI KNIGVEHIVD MIKKIMDNVQ GRVHGFHFCT LNLERSVALI
310 320 330 340 350
LKNSGLLTKR WKQVESEMED EKLLRTTRKR LSLDEPAELH NQVVVPSQQP
360 370 380 390 400
VADKSSNLFV TSKQSSVSGH KDNLTEEAPF SVSEGSGVLG RQANWDDFTN
410 420 430 440 450
GRFGDPRSPA YGEIDGYGPT LHFPPSEALK LWGYPVDESD ITSLFQKHIM
460 470 480 490 500
SDISAIPWID EPVEVETKTI AKYLLKLNGN SWWTVGSQPA VNGAPSADPV
510 520 530 540 550
FGWGPKGGRV FQKAFVECFV NGKDLKDFIT KWHDNPQVTY YAGNNKSEFL
560 570 580 590 600
TNAPKDGASA VTWGVYPGRE IIQSTIIAEV SFKAWLSESF QVWGEWANLY
610 620 630 640
SKNTPSRKLL ENCINDRWLV TVIHHDFMDK NGLWKVLEID F
Length:641
Mass (Da):72,140
Last modified:August 14, 2001 - v2
Checksum:iE7AFFDD1F2C1C8A4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti202 – 2021E → D in BAA13780. (PubMed:9501991)Curated
Sequence conflicti281 – 2811G → A in BAA13780. (PubMed:9501991)Curated
Sequence conflicti455 – 4551A → V in BAA13780. (PubMed:9501991)Curated
Sequence conflicti537 – 641105QVTYY…LEIDF → HVRSNFSSSISDSTCFQRLV SKPLFLRIRATDRSKFSVQK in BAA13780. (PubMed:9501991)CuratedAdd
BLAST
Sequence conflicti614 – 6141I → L in CAA09738. (PubMed:12112238)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ011686 Genomic DNA. Translation: CAA09738.1.
CU329670 Genomic DNA. Translation: CAB52273.1.
D89118 mRNA. Translation: BAA13780.1.
PIRiT38659.
RefSeqiNP_593430.1. NM_001018863.2.

Genome annotation databases

EnsemblFungiiSPAC343.10.1; SPAC343.10.1:pep; SPAC343.10.
GeneIDi2543105.
KEGGispo:SPAC343.10.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ011686 Genomic DNA. Translation: CAA09738.1 .
CU329670 Genomic DNA. Translation: CAB52273.1 .
D89118 mRNA. Translation: BAA13780.1 .
PIRi T38659.
RefSeqi NP_593430.1. NM_001018863.2.

3D structure databases

ProteinModelPortali O74927.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 279537. 7 interactions.
STRINGi 4896.SPAC343.10-1.

Proteomic databases

MaxQBi O74927.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC343.10.1 ; SPAC343.10.1:pep ; SPAC343.10 .
GeneIDi 2543105.
KEGGi spo:SPAC343.10.

Organism-specific databases

PomBasei SPAC343.10.

Phylogenomic databases

eggNOGi COG0685.
HOGENOMi HOG000246234.
InParanoidi O74927.
KOi K00297.
OMAi NVTWGAG.
OrthoDBi EOG7M0P15.
PhylomeDBi O74927.

Enzyme and pathway databases

UniPathwayi UPA00193 .

Miscellaneous databases

NextBioi 20804132.

Family and domain databases

Gene3Di 3.20.20.220. 1 hit.
InterProi IPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view ]
Pfami PF02219. MTHFR. 1 hit.
[Graphical view ]
SUPFAMi SSF51730. SSF51730. 1 hit.
TIGRFAMsi TIGR00677. fadh2_euk. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Two non-complementing genes encoding enzymatically active methylenetetrahydrofolate reductases control methionine requirement in fission yeast Schizosaccharomyces pombe."
    Naula N., Walther C., Baumann D., Schweingruber M.E.
    Yeast 19:841-848(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Identification of open reading frames in Schizosaccharomyces pombe cDNAs."
    Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.
    DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 11-641.
    Strain: PR745.

Entry informationi

Entry nameiMTHR2_SCHPO
AccessioniPrimary (citable) accession number: O74927
Secondary accession number(s): P78770, Q9UT80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 14, 2001
Last modified: October 29, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3