O74922 (AMY1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-amylase 1
EC=3.2.1.1

Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase

Gene names

Name:	aah1
ORF Names:	SPCC757.12

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	625 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.
Cofactor	Binds 2 calcium ions per subunit. Calcium is inhibitory at high concentrations By similarity.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular polysaccharide catabolic process Inferred by curator. Source: GeneDB_Spombe extracellular polysaccharide metabolic process Inferred by curator. Source: GeneDB_Spombe fungal-type cell wall biogenesis Inferred from sequence or structural similarity. Source: GeneDB_Spombe regulation of cell shape Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW cell surface Inferred by curator. Source: GeneDB_Spombe cell wall Inferred from sequence or structural similarity. Source: GeneDB_Spombe endoplasmic reticulum Inferred from direct assay. Source: GeneDB_Spombe plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-amylase activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 603	581	Alpha-amylase 1		PRO_0000374015
	Propeptide	604 – 625	22	Removed in mature form Potential		PRO_0000374016
Regions
	Region	232 – 233	2	Substrate binding By similarity
	Compositional bias	502 – 604	103	Ser-rich
Sites
	Active site	229	1	Nucleophile By similarity
	Active site	253	1	Proton donor By similarity
	Metal binding	143	1	Calcium 1 By similarity
	Metal binding	185	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	198	1	Calcium 1 By similarity
	Metal binding	229	1	Calcium 2 By similarity
	Metal binding	253	1	Calcium 2 By similarity
	Binding site	105	1	Substrate By similarity
	Binding site	227	1	Substrate By similarity
	Binding site	321	1	Substrate By similarity
	Binding site	370	1	Substrate By similarity
	Site	322	1	Transition state stabilizer By similarity
Amino acid modifications
	Lipidation	603	1	GPI-anchor amidated serine Potential
	Glycosylation	153	1	N-linked (GlcNAc...) Potential
	Glycosylation	163	1	N-linked (GlcNAc...) Potential
	Glycosylation	180	1	N-linked (GlcNAc...) Potential
	Glycosylation	241	1	N-linked (GlcNAc...) Potential
	Glycosylation	260	1	N-linked (GlcNAc...) Potential
	Glycosylation	286	1	N-linked (GlcNAc...) Potential
	Glycosylation	331	1	N-linked (GlcNAc...) Potential
	Glycosylation	440	1	N-linked (GlcNAc...) Potential
	Glycosylation	461	1	N-linked (GlcNAc...) Potential
	Disulfide bond	51 ↔ 59		By similarity
	Disulfide bond	172 ↔ 187		By similarity
	Disulfide bond	263 ↔ 306		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O74922 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 30A58CE6D6BC07B3	FASTA	625	68,087
	10 20 30 40 50 60 MGFSKIALFS LFALFGLPTS LAKSSEEWRD RIIYQVITDR FAVDSDNTPD CSFDDSSYCG 70 80 90 100 110 120 GTWSGIRSKL DYIQGMGFNA IWISPVEKNL EGSYGSDGEA YHGYWNTDFT QLNEHFGSED 130 140 150 160 170 180 DLIDLITDMH NRDMWIMFDA LANSMAIPGP TDNISYSNLV PFNDSSYFHP YCWIDYGSNN 190 200 210 220 230 240 NTDIEDCWTG DDNVILADLD IESTNVADYL HEHIHDMVER YQIDGIRIDA VKQMNPEFFP 250 260 270 280 290 300 NYTSAAGVFA IGEMFSYDPN VSCSVRNYLD SITSYPIRQG IEFAFNYTGA AFEYLQEIDT 310 320 330 340 350 360 QFQQACEGQD MSVIGNFLEN HDLPRYTSIT NDTSQDIGAI VFLLLHTGIP IIYYGEEQRL 370 380 390 400 410 420 PGGSDTPENR AALWNYGYDT DANYYQTIRT AIALRKQAIS DSDSWTTDSH SYLDYDLRHA 430 440 450 460 470 480 VVRKGDVLGV YTNYESSSDN VTYDVSSNFD DGTVLREVLS NTTTTVGSSG ALHVTVVSGL 490 500 510 520 530 540 PQVYYPEASL TSFGNFLGTA TSYSSASASY PSTSMSASLS SVHTSSATSS SKSSSSSSSR 550 560 570 580 590 600 SGSSSSSSSR SGSTSSSGSS HTITSTSQSV HTSGSSTSTS SVAVTSTAYS SSSSSSSSSS 610 620 IESSANAVRV SILGVAAFIA IVLFI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"An alpha-amylase homologue, aah3, encodes a GPI-anchored membrane protein required for cell wall integrity and morphogenesis in Schizosaccharomyces pombe." Morita T., Tanaka N., Hosomi A., Giga-Hama Y., Takegawa K. Biosci. Biotechnol. Biochem. 70:1454-1463(2006) [PubMed: 16751704] [Abstract] Cited for: GENE NAME.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329672 Genomic DNA. Translation: CAA21237.1.
PIR	T41603.
RefSeq	NP_587687.1. NM_001022682.1.
3D structure databases
HSSP	HSSP built from PDB template 2AAA based on UniProtKB P56271.
ProteinModelPortal	O74922.
ModBase	Search...
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPCC757.12.1; SPCC757.12.1:pep; SPCC757.12.
GeneID	2538825.
GenomeReviews	Gene locus aah1 in contig CU329672_GR.
KEGG	spo:SPCC757.12.
NMPDR	fig|4896.1.peg.25.
Organism-specific databases
GeneDB_Spombe	SPCC757.12.
Phylogenomic databases
eggNOG	fuNOG06463.
GeneTree	EFGT00050000000134.
HOGENOM	HBG324773.
OrthoDB	EOG4V1B83.
Gene expression databases
ArrayExpress	O74922.
Family and domain databases
InterPro	IPR013777. A-amylase_fun. IPR015340. A_amylase_DUF1966_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR006589. Glyco_hydro_13_sub_cat_dom. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KO	K01176.
PANTHER	PTHR10357. Alpha_amylase. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF09260. DUF1966. 1 hit. [Graphical view]
PIRSF	PIRSF001024. Alph-amyl_fung. 1 hit.
SMART	SM00642. Aamy. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AMY1_SCHPO

Accession

Primary (citable) accession number: O74922

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	November 1, 1998
Last modified:	December 14, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program