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Protein

Alpha-amylase 1

Gene

aah1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

Cofactori

Ca2+By similarityNote: Binds 2 calcium ions per subunit. Calcium is inhibitory at high concentrations.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei105SubstrateBy similarity1
Metal bindingi143Calcium 1By similarity1
Metal bindingi185Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi198Calcium 1By similarity1
Binding sitei227SubstrateBy similarity1
Active sitei229NucleophileBy similarity1
Metal bindingi229Calcium 2By similarity1
Active sitei253Proton donorBy similarity1
Metal bindingi253Calcium 2By similarity1
Binding sitei322SubstrateBy similarity1
Sitei322Transition state stabilizerBy similarity1
Binding sitei370SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandCalcium, Metal-binding

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase 1 (EC:3.2.1.1)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:aah1
ORF Names:SPCC757.12
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC757.12
PomBaseiSPCC757.12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000037401523 – 603Alpha-amylase 1Add BLAST581
PropeptideiPRO_0000374016604 – 625Removed in mature formSequence analysisAdd BLAST22

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi51 ↔ 59By similarity
Glycosylationi153N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi163N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi172 ↔ 187By similarity
Glycosylationi180N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi241N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi260N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi263 ↔ 306By similarity
Glycosylationi286N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi331N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi440N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi461N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi603GPI-anchor amidated serineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiO74922
PaxDbiO74922
PRIDEiO74922

Interactioni

Protein-protein interaction databases

BioGridi2754069 interactors.
STRINGi4896.SPCC757.12.1

Structurei

3D structure databases

ProteinModelPortaliO74922
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni232 – 233Substrate bindingBy similarity2

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi502 – 604Ser-richAdd BLAST103

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000165530
InParanoidiO74922
KOiK01176
OMAiHTTWIKS
OrthoDBiEOG092C1HLH
PhylomeDBiO74922

Family and domain databases

Gene3Di2.60.40.11801 hit
InterProiView protein in InterPro
IPR013777 A-amylase-like
IPR015340 A_amylase_DUF1966_C
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF09260 DUF1966, 1 hit
PIRSFiPIRSF001024 Alph-amyl_fung, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O74922-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFSKIALFS LFALFGLPTS LAKSSEEWRD RIIYQVITDR FAVDSDNTPD
60 70 80 90 100
CSFDDSSYCG GTWSGIRSKL DYIQGMGFNA IWISPVEKNL EGSYGSDGEA
110 120 130 140 150
YHGYWNTDFT QLNEHFGSED DLIDLITDMH NRDMWIMFDA LANSMAIPGP
160 170 180 190 200
TDNISYSNLV PFNDSSYFHP YCWIDYGSNN NTDIEDCWTG DDNVILADLD
210 220 230 240 250
IESTNVADYL HEHIHDMVER YQIDGIRIDA VKQMNPEFFP NYTSAAGVFA
260 270 280 290 300
IGEMFSYDPN VSCSVRNYLD SITSYPIRQG IEFAFNYTGA AFEYLQEIDT
310 320 330 340 350
QFQQACEGQD MSVIGNFLEN HDLPRYTSIT NDTSQDIGAI VFLLLHTGIP
360 370 380 390 400
IIYYGEEQRL PGGSDTPENR AALWNYGYDT DANYYQTIRT AIALRKQAIS
410 420 430 440 450
DSDSWTTDSH SYLDYDLRHA VVRKGDVLGV YTNYESSSDN VTYDVSSNFD
460 470 480 490 500
DGTVLREVLS NTTTTVGSSG ALHVTVVSGL PQVYYPEASL TSFGNFLGTA
510 520 530 540 550
TSYSSASASY PSTSMSASLS SVHTSSATSS SKSSSSSSSR SGSSSSSSSR
560 570 580 590 600
SGSTSSSGSS HTITSTSQSV HTSGSSTSTS SVAVTSTAYS SSSSSSSSSS
610 620
IESSANAVRV SILGVAAFIA IVLFI
Length:625
Mass (Da):68,087
Last modified:November 1, 1998 - v1
Checksum:i30A58CE6D6BC07B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA Translation: CAA21237.1
PIRiT41603
RefSeqiNP_587687.1, NM_001022682.2

Genome annotation databases

EnsemblFungiiSPCC757.12.1; SPCC757.12.1:pep; SPCC757.12
GeneIDi2538825
KEGGispo:SPCC757.12

Similar proteinsi

Entry informationi

Entry nameiAMY1_SCHPO
AccessioniPrimary (citable) accession number: O74922
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: November 1, 1998
Last modified: February 28, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome