aah1

Functionⁱ

Catalytic activityⁱ

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

Cofactorⁱ

Ca²⁺By similarityNote: Binds 2 calcium ions per subunit. Calcium is inhibitory at high concentrations.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	105	SubstrateBy similarity	1
Metal bindingⁱ	143	Calcium 1By similarity	1
Metal bindingⁱ	185	Calcium 1; via carbonyl oxygenBy similarity	1
Metal bindingⁱ	198	Calcium 1By similarity	1
Binding siteⁱ	227	SubstrateBy similarity	1
Active siteⁱ	229	NucleophileBy similarity	1
Metal bindingⁱ	229	Calcium 2By similarity	1
Active siteⁱ	253	Proton donorBy similarity	1
Metal bindingⁱ	253	Calcium 2By similarity	1
Binding siteⁱ	322	SubstrateBy similarity	1
Siteⁱ	322	Transition state stabilizerBy similarity	1
Binding siteⁱ	370	SubstrateBy similarity	1

GO - Molecular functionⁱ

alpha-amylase activity Source: UniProtKB-EC
alpha-amylase activity (releasing maltohexaose) Source: UniProtKB-EC
calcium ion binding Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

carbohydrate catabolic process Source: InterPro
fungal-type cell wall biogenesis Source: PomBase

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Glycosidase, Hydrolase
Biological process	Carbohydrate metabolism
Ligand	Calcium, Metal-binding

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	GH13 Glycoside Hydrolase Family 13

CAZyⁱ

GH13 Glycoside Hydrolase Family 13

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Alpha-amylase 1 (EC:3.2.1.1) Alternative name(s): 1,4-alpha-D-glucan glucanohydrolase
Gene namesⁱ	Name:aah1 ORF Names:SPCC757.12
Organismⁱ	Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifierⁱ	284812 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe
Proteomesⁱ	UP000002485 Componentⁱ: Chromosome III

Organism-specific databases

EuPathDBⁱ	FungiDB:SPCC757.12
PomBaseⁱ	SPCC757.12

Keywords - Cellular componentⁱ

Cell membrane, Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Length
Signal peptideⁱ	1 – 22	Sequence analysisAdd BLAST	22
ChainⁱPRO_0000374015	23 – 603	Alpha-amylase 1Add BLAST	581
PropeptideⁱPRO_0000374016	604 – 625	Removed in mature formSequence analysisAdd BLAST	22

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Disulfide bondⁱ	51 ↔ 59	By similarity
Glycosylationⁱ	153	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	163	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	172 ↔ 187	By similarity
Glycosylationⁱ	180	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	241	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	260	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	263 ↔ 306	By similarity
Glycosylationⁱ	286	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	331	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	440	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	461	N-linked (GlcNAc...) asparagineSequence analysis	1
Lipidationⁱ	603	GPI-anchor amidated serineSequence analysis	1

Keywords - PTMⁱ

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O74922
PaxDbⁱ	O74922
PRIDEⁱ	O74922

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	2754069 interactors.
STRINGⁱ	4896.SPCC757.12.1

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O74922
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	232 – 233	Substrate bindingBy similarity		2

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	502 – 604	Ser-richAdd BLAST		103

Sequence similaritiesⁱ

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domainⁱ

Signal

Phylogenomic databases

HOGENOMⁱ	HOG000165530
InParanoidⁱ	O74922
KEGG Orthology (KO) More...KOⁱ	K01176
OMAⁱ	HTTWIKS
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C1HLH
PhylomeDBⁱ	O74922

Family and domain databases

Gene3Dⁱ	2.60.40.11801 hit
InterProⁱ	View protein in InterPro IPR013777 A-amylase-like IPR015340 A_amylase_DUF1966_C IPR006047 Glyco_hydro_13_cat_dom IPR013780 Glyco_hydro_b IPR017853 Glycoside_hydrolase_SF
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00128 Alpha-amylase, 1 hit PF09260 DUF1966, 1 hit
PIRSFⁱ	PIRSF001024 Alph-amyl_fung, 1 hit
SMARTⁱ	View protein in SMART SM00642 Aamy, 1 hit
SUPFAMⁱ	SSF51445 SSF51445, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

O74922-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MGFSKIALFS LFALFGLPTS LAKSSEEWRD RIIYQVITDR FAVDSDNTPD 
        60         70         80         90        100
CSFDDSSYCG GTWSGIRSKL DYIQGMGFNA IWISPVEKNL EGSYGSDGEA 
       110        120        130        140        150
YHGYWNTDFT QLNEHFGSED DLIDLITDMH NRDMWIMFDA LANSMAIPGP 
       160        170        180        190        200
TDNISYSNLV PFNDSSYFHP YCWIDYGSNN NTDIEDCWTG DDNVILADLD 
       210        220        230        240        250
IESTNVADYL HEHIHDMVER YQIDGIRIDA VKQMNPEFFP NYTSAAGVFA 
       260        270        280        290        300
IGEMFSYDPN VSCSVRNYLD SITSYPIRQG IEFAFNYTGA AFEYLQEIDT 
       310        320        330        340        350
QFQQACEGQD MSVIGNFLEN HDLPRYTSIT NDTSQDIGAI VFLLLHTGIP 
       360        370        380        390        400
IIYYGEEQRL PGGSDTPENR AALWNYGYDT DANYYQTIRT AIALRKQAIS 
       410        420        430        440        450
DSDSWTTDSH SYLDYDLRHA VVRKGDVLGV YTNYESSSDN VTYDVSSNFD 
       460        470        480        490        500
DGTVLREVLS NTTTTVGSSG ALHVTVVSGL PQVYYPEASL TSFGNFLGTA 
       510        520        530        540        550
TSYSSASASY PSTSMSASLS SVHTSSATSS SKSSSSSSSR SGSSSSSSSR 
       560        570        580        590        600
SGSTSSSGSS HTITSTSQSV HTSGSSTSTS SVAVTSTAYS SSSSSSSSSS 
       610        620 
IESSANAVRV SILGVAAFIA IVLFI

Length:625

Mass (Da):68,087

Last modified:November 1, 1998 - v1

Checksum:ⁱ30A58CE6D6BC07B3

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CU329672 Genomic DNA Translation: CAA21237.1
PIRⁱ	T41603
NCBI Reference Sequences More...RefSeqⁱ	NP_587687.1, NM_001022682.2

Genome annotation databases

EnsemblFungiⁱ	SPCC757.12.1; SPCC757.12.1:pep; SPCC757.12
GeneIDⁱ	2538825
KEGGⁱ	spo:SPCC757.12

Similar proteinsⁱ

50% Identity

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
O74922	S9QVP2 S9W0K2	Schizosaccharomyces octosporus (strain yFS286) (Fission yeast) (Octosporomyces octosporus) Schizosaccharomyces cryophilus (strain OY26 / ATCC MYA-4695 / CBS 11777 / NBRC 106824 / NRRL Y48691) (Fission yeast)	625	UniRef50_O74922	Cluster: Alpha-amylase 1	3

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CU329672 Genomic DNA Translation: CAA21237.1
PIRⁱ	T41603
NCBI Reference Sequences More...RefSeqⁱ	NP_587687.1, NM_001022682.2

3D structure databases

ProteinModelPortalⁱ	O74922
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	2754069 interactors.
STRINGⁱ	4896.SPCC757.12.1

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	GH13 Glycoside Hydrolase Family 13

CAZyⁱ

GH13 Glycoside Hydrolase Family 13

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O74922
PaxDbⁱ	O74922
PRIDEⁱ	O74922

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	SPCC757.12.1; SPCC757.12.1:pep; SPCC757.12
GeneIDⁱ	2538825
KEGGⁱ	spo:SPCC757.12

Organism-specific databases

EuPathDBⁱ	FungiDB:SPCC757.12
PomBaseⁱ	SPCC757.12

Phylogenomic databases

HOGENOMⁱ	HOG000165530
InParanoidⁱ	O74922
KEGG Orthology (KO) More...KOⁱ	K01176
OMAⁱ	HTTWIKS
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C1HLH
PhylomeDBⁱ	O74922

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:O74922

PROⁱ

PR:O74922

Family and domain databases

Gene3Dⁱ	2.60.40.11801 hit
InterProⁱ	View protein in InterPro IPR013777 A-amylase-like IPR015340 A_amylase_DUF1966_C IPR006047 Glyco_hydro_13_cat_dom IPR013780 Glyco_hydro_b IPR017853 Glycoside_hydrolase_SF
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00128 Alpha-amylase, 1 hit PF09260 DUF1966, 1 hit
PIRSFⁱ	PIRSF001024 Alph-amyl_fung, 1 hit
SMARTⁱ	View protein in SMART SM00642 Aamy, 1 hit
SUPFAMⁱ	SSF51445 SSF51445, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	AMY1_SCHPO
Accessionⁱ	O74922Primary (citable) accession number: O74922
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
	Last sequence update:	November 1, 1998
	Last modified:	February 28, 2018
	This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - O74922 (AMY1_SCHPO)

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Alpha-amylase 1

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Cell Wall

Endoplasmic reticulum

Plasma Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ