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Protein

Diphthine methyl ester synthase

Gene

dph5

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.By similarity

Catalytic activityi

4 S-adenosyl-L-methionine + 2-((3S)-3-carboxy-3-aminopropyl)-L-histidine-[translation elongation factor 2] = 4 S-adenosyl-L-homocysteine + diphthine methyl ester-[translation elongation factor 2].By similarity

Pathwayi: peptidyl-diphthamide biosynthesis

This protein is involved in the pathway peptidyl-diphthamide biosynthesis, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway peptidyl-diphthamide biosynthesis and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 91S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
Binding sitei84 – 841S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei87 – 871S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
Binding sitei163 – 1631S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei221 – 2211S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei246 – 2461S-adenosyl-L-methionine; via carbonyl oxygenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-SPO-5358493. Synthesis of diphthamide-EEF2.
UniPathwayiUPA00559.

Names & Taxonomyi

Protein namesi
Recommended name:
Diphthine methyl ester synthase (EC:2.1.1.314)
Alternative name(s):
Diphthamide biosynthesis methyltransferase
Gene namesi
Name:dph5
ORF Names:SPCC576.14
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC576.14.
PomBaseiSPCC576.14. dph5.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 283283Diphthine methyl ester synthasePRO_0000156145Add
BLAST

Proteomic databases

MaxQBiO74898.

Interactioni

Protein-protein interaction databases

BioGridi276034. 18 interactions.
MINTiMINT-4681683.

Structurei

3D structure databases

ProteinModelPortaliO74898.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni112 – 1132S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Belongs to the diphthine synthase family.Curated

Phylogenomic databases

HOGENOMiHOG000205302.
InParanoidiO74898.
KOiK00586.
OMAiMTANEGL.
OrthoDBiEOG092C4D28.
PhylomeDBiO74898.

Family and domain databases

CDDicd11647. Diphthine_synthase. 1 hit.
Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
HAMAPiMF_01084. Diphthine_synth. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR004551. Dphthn_synthase.
[Graphical view]
PANTHERiPTHR10882:SF0. PTHR10882:SF0. 1 hit.
PfamiPF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036432. Diphthine_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR00522. dph5. 1 hit.

Sequencei

Sequence statusi: Complete.

O74898-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFYLIGLGLF DEKDITLRGL ETVKKCQRIY LEAYTSILLV QKEKLEELYG
60 70 80 90 100
KEVILADREM VESSSDEILK DADNCDVAML VVGDPMGATT HADLVIRARE
110 120 130 140 150
LKIPVRMIHN ASIMNAIGAC GLQLYKFGQT VSLVFFENNY RPQSFYDHIK
160 170 180 190 200
ENVSLGLHTL VLLDIKVKEQ SWENLARGRK VYEPPRYMSA SLAAQQMLEV
210 220 230 240 250
EEDRQENICT PDSLCVAVGR MGSDDQVIFA GTLQELAEHD IGPPLHSVVL
260 270 280
VGRDVHDLEL EFLRAYAVNL ENFDRVAKTY LEK
Length:283
Mass (Da):32,044
Last modified:November 1, 1998 - v1
Checksum:iFF3300910CEC1AD7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA21193.1.
PIRiT41424.
RefSeqiNP_588441.1. NM_001023432.2.

Genome annotation databases

EnsemblFungiiSPCC576.14.1; SPCC576.14.1:pep; SPCC576.14.
GeneIDi2539471.
KEGGispo:SPCC576.14.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA21193.1.
PIRiT41424.
RefSeqiNP_588441.1. NM_001023432.2.

3D structure databases

ProteinModelPortaliO74898.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276034. 18 interactions.
MINTiMINT-4681683.

Proteomic databases

MaxQBiO74898.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC576.14.1; SPCC576.14.1:pep; SPCC576.14.
GeneIDi2539471.
KEGGispo:SPCC576.14.

Organism-specific databases

EuPathDBiFungiDB:SPCC576.14.
PomBaseiSPCC576.14. dph5.

Phylogenomic databases

HOGENOMiHOG000205302.
InParanoidiO74898.
KOiK00586.
OMAiMTANEGL.
OrthoDBiEOG092C4D28.
PhylomeDBiO74898.

Enzyme and pathway databases

UniPathwayiUPA00559.
ReactomeiR-SPO-5358493. Synthesis of diphthamide-EEF2.

Miscellaneous databases

PROiO74898.

Family and domain databases

CDDicd11647. Diphthine_synthase. 1 hit.
Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
HAMAPiMF_01084. Diphthine_synth. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR004551. Dphthn_synthase.
[Graphical view]
PANTHERiPTHR10882:SF0. PTHR10882:SF0. 1 hit.
PfamiPF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036432. Diphthine_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR00522. dph5. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDPH5_SCHPO
AccessioniPrimary (citable) accession number: O74898
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: November 1, 1998
Last modified: September 7, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.