Reviewed, UniProtKB/Swiss-Prot O74890 (SYYM_SCHPO)

Names and origin

Protein names

Recommended name:
    Tyrosyl-tRNA synthetase, mitochondrial
    EC=6.1.1.1
Alternative name(s):
    Tyrosine--tRNA ligase
      Short name=TyrRS

Gene names

Name:	msy1
ORF Names:	SPCC576.06c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity.
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). UniProtKB P48527
Subcellular location	Mitochondrion matrix. Ref.2 UniProtKB P48527
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential UniProtKB P48527
	Chain	? – 445		Tyrosyl-tRNA synthetase, mitochondrial UniProtKB P48527		PRO_0000314632
Regions
	Domain	384 – 445	62	S4 RNA-binding
	Motif	38 – 47	10	"HIGH" region
	Motif	245 – 249	5	"KMSKS" region
Sites
	Binding site	248	1	ATP By similarity UniProtKB P83453

Sequences

	Sequence		Length	Mass (Da)
	O74890-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: E47BD594369C7EE2	FASTA	445	49,498
	10 20 30 40 50 60 MSRLLACLKQ LQARSLIHNT TLLQPSCNVN SVYLGADPTA ASLHVGNLVA LMPLVHFFLN 70 80 90 100 110 120 GFPVFTVIGD ATAQLGDPSG RSTSRKQMAE TTRTANSNSI HNQLKDLSSS ILSYAQDCNY 130 140 150 160 170 180 PFSQMPSSSQ WSIVRNSSWY ENLKLLKFLS SVGPHVRVSQ MLARDSVTTR LQSPSGLSFA 190 200 210 220 230 240 ELTYQLLQAY DYSYLYENHS VNLQIGGSDQ WGNITAGTDL VRRTHPNANV YALTTPLLTS 250 260 270 280 290 300 SSGQKLGKSA GNAIWLDPKL TDSYSLYQYF ISAPDDLACK CLDMLTLLPL EQLEQIKAEH 310 320 330 340 350 360 EKDPSQRIVH KYLASNVVRM VHGKKALELA QIQTKLLHGA HQAPFGFYSE APQQGDSFPS 370 380 390 400 410 420 LPEIRALFKD CKFYRTIDSS IKDQPFSRLL RTLQIYTSRK EATEHILSGA VSLGHKPILD 430 440 SNYKFPDNSL FVLRAGKRTF VLDSL

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

[2]

"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329672 Genomic DNA. Translation: CAA21185.1.
PIR	T41416.
RefSeq	NP_588433.1.
3D structure databases
HSSP	HSSP built from PDB template 1TYA based on UniProtKB P00952.
ModBase	Search...
Protein-protein interaction databases
STRING	O74890.
Genome annotation databases
GeneID	2539529.
GenomeReviews	Gene locus msy1 in contig CU329672_GR.
KEGG	spo:SPCC576.06c.
NMPDR	fig|4896.1.peg.771.
Organism-specific databases
GeneDB_Spombe	SPCC576.06c.
Phylogenomic databases
OMA	ISLYCGV.
Enzyme and pathway databases
BRENDA	6.1.1.1. 653.
Gene expression databases
ArrayExpress	O74890.
Family and domain databases
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ib. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA_bd. IPR002307. Tyr-tRNA-synth_Ib_bac/mito. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00234. tyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYYM_SCHPO

Accession

Primary (citable) accession number: O74890

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 1, 1998
Last modified:	November 3, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)