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Protein

mRNA cap guanine-N7 methyltransferase

Gene

pcm1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for methylating the 5'-cap structure of mRNAs.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei96 – 961S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei118 – 1181S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Sitei121 – 1211mRNA cap bindingPROSITE-ProRule annotation
Sitei127 – 1271mRNA cap bindingPROSITE-ProRule annotation
Binding sitei140 – 1401S-adenosyl-L-methioninePROSITE-ProRule annotation
Sitei152 – 1521mRNA cap bindingPROSITE-ProRule annotation
Binding sitei195 – 1951mRNA capPROSITE-ProRule annotation
Binding sitei283 – 2831mRNA capPROSITE-ProRule annotation
Binding sitei350 – 3501mRNA capPROSITE-ProRule annotation

GO - Molecular functioni

  • mRNA (guanine-N7-)-methyltransferase activity Source: PomBase
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • 7-methylguanosine mRNA capping Source: PomBase
  • RNA methylation Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-SPO-72086. mRNA Capping.
R-SPO-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA cap guanine-N7 methyltransferase (EC:2.1.1.56)
Alternative name(s):
mRNA (guanine-N(7)-)-methyltransferase
mRNA cap methyltransferase
Gene namesi
Name:pcm1
ORF Names:SPCC330.10
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC330.10.
PomBaseiSPCC330.10. pcm1.

Subcellular locationi

GO - Cellular componenti

  • cell division site Source: PomBase
  • cytosol Source: PomBase
  • DNA-directed RNA polymerase II, holoenzyme Source: PomBase
  • mRNA cap binding complex Source: GO_Central
  • nucleus Source: PomBase
  • P-TEFb-cap methyltransferase complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 360360mRNA cap guanine-N7 methyltransferasePRO_0000303915Add
BLAST

Proteomic databases

MaxQBiO74880.

Interactioni

Subunit structurei

Interacts with cdk9.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
pch1O746272EBI-7296037,EBI-443575

Protein-protein interaction databases

BioGridi275848. 3 interactions.
IntActiO74880. 1 interaction.
MINTiMINT-4543591.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini44 – 360317mRNA cap 0 methyltransferasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni92 – 932mRNA cap bindingPROSITE-ProRule annotation
Regioni168 – 1692S-adenosyl-L-methionine bindingPROSITE-ProRule annotation
Regioni191 – 1933S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family.PROSITE-ProRule annotation
Contains 1 mRNA cap 0 methyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000242614.
InParanoidiO74880.
KOiK00565.
OMAiCFGESLG.
OrthoDBiEOG092C2SMS.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR016899. mRNA_G-N7_MeTrfase.
IPR004971. mRNA_G-N7_MeTrfase_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF03291. Pox_MCEL. 1 hit.
[Graphical view]
PIRSFiPIRSF028762. ABD1. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51562. RNA_CAP0_MT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O74880-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSNSRVHE EQPPTENRRY ARPTAQMNRV IEQQPRRRDY FQNNDNSGRR
60 70 80 90 100
GYNRHENNGN AQDVVRSHYN ARPDLGYKKR QFSPIIQLKR FNNWIKSVLI
110 120 130 140 150
QKFAPHASDY PILVLDMGCG KGGDLIKWDK AGIDGYIGID IAEVSVNQAK
160 170 180 190 200
KRYREMHASF DALFYAGDCF SSSINELLPP DQRKFDVVSL QFCMHYAFES
210 220 230 240 250
EEKVRVLLGN VSKCLPRGGV MIGTIPNSDV IVKHIKMLKP GEKEWGNDIY
260 270 280 290 300
KVRFPESPPR SFRPPYGIQY YFYLEDAVTD VPEYVVPFEA FRAVAEGYNL
310 320 330 340 350
ELIWVKPFLD ILNEEKNSET YGPLMDRMKV VDNEGHRGIG GQEKEAAGFY
360
LAFAFEKRGI
Length:360
Mass (Da):41,441
Last modified:July 11, 2012 - v2
Checksum:i78C33DC6919FD242
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA20915.2.
PIRiT41320.
RefSeqiNP_587710.2. NM_001022705.2.

Genome annotation databases

EnsemblFungiiSPCC330.10.1; SPCC330.10.1:pep; SPCC330.10.
GeneIDi2539280.
KEGGispo:SPCC330.10.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA20915.2.
PIRiT41320.
RefSeqiNP_587710.2. NM_001022705.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275848. 3 interactions.
IntActiO74880. 1 interaction.
MINTiMINT-4543591.

Proteomic databases

MaxQBiO74880.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC330.10.1; SPCC330.10.1:pep; SPCC330.10.
GeneIDi2539280.
KEGGispo:SPCC330.10.

Organism-specific databases

EuPathDBiFungiDB:SPCC330.10.
PomBaseiSPCC330.10. pcm1.

Phylogenomic databases

HOGENOMiHOG000242614.
InParanoidiO74880.
KOiK00565.
OMAiCFGESLG.
OrthoDBiEOG092C2SMS.

Enzyme and pathway databases

ReactomeiR-SPO-72086. mRNA Capping.
R-SPO-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Miscellaneous databases

PROiO74880.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR016899. mRNA_G-N7_MeTrfase.
IPR004971. mRNA_G-N7_MeTrfase_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF03291. Pox_MCEL. 1 hit.
[Graphical view]
PIRSFiPIRSF028762. ABD1. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51562. RNA_CAP0_MT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMCES_SCHPO
AccessioniPrimary (citable) accession number: O74880
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 11, 2012
Last modified: September 7, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.