O74878 (ALG11_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase
EC=2.4.1.131

Alternative name(s):
Alpha-1,2-mannosyltransferase alg11
Asparagine-linked glycosylation protein 11
Galactomannan deficiency protein 3
Glycolipid 2-alpha-mannosyltransferase

Gene names

Name:	alg11
Synonyms:	gmd3
ORF Names:	SPCC330.08

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Required for N-linked oligosaccharide assembly. Has a role in the last step of the synthesis of the Man5GlcNAc(2)-PP-dolichol core oligosaccharide on the cytoplasmic face of the endoplasmic reticulum. Ref.1
Catalytic activity	2 GDP-D-mannose + D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = 2 GDP + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	oligosaccharide-lipid intermediate biosynthetic process Inferred from mutant phenotype. Source: GeneDB_Spombe
Cellular component	endoplasmic reticulum membrane Inferred from genetic interaction. Source: GeneDB_Spombe integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GDP-Man:Man3GlcNAc2-PP-Dol alpha-1,2-mannosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase		PRO_0000080278
Regions
	Transmembrane	1 – 25	25	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	26 – 471	446	Lumenal Potential
Amino acid modifications
	Glycosylation	97	1	N-linked (GlcNAc...) Potential
	Glycosylation	236	1	N-linked (GlcNAc...) Potential
	Glycosylation	262	1	N-linked (GlcNAc...) Potential
	Glycosylation	390	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O74878 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 80D30A980D789366	FASTA	471	53,278
	10 20 30 40 50 60 MITTLAIALF IAVVAIHSHI RRKICKEIGI RLIKKIAPVK ASLYKQVGVE PKLARTVGFF 70 80 90 100 110 120 HPYCNAGGGG ERVLWTAVKS VQTEFPNVIS VVYTGDNVSK AEILRRVKNT FEIDLDSSKI 130 140 150 160 170 180 VFVYLKLRFL VSATTWHRFT LLGQSLGSMI LGFEAIYRFA PDIFIDTMGY AFTFCVVKSF 190 200 210 220 230 240 QNIPVGAYVH YPTISTDMLK SLKQVSLLAK VKMAYWRWFA QLYSDAGSHA DYVMTNSSWT 250 260 270 280 290 300 RNHIASLWGK DIQLSVVFPP CNTSELEKID INRKREPTLL YLAQYRPEKN HENVLRSFAL 310 320 330 340 350 360 YFEQHPDSPA KLLLVGSVRG EEDMCFVNHL KTLATELNLQ SKVKFVVDAP WPKVVEYLGT 370 380 390 400 410 420 CSIGVNYMWN EHFGIGVVEY MAAGLIPVVN NSGGPKFDIV IPWIGKPTGF HASTISEYAE 430 440 450 460 470 AYHKALTLSP QEQLEMRINA RSACARFGEH VFMRDFGNVF AKLLREDYSR T

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Schizosaccharomyces pombe gmd3(+)/alg11(+) is a functional homologue of Saccharomyces cerevisiae ALG11 which is involved in N-linked oligosaccharide synthesis." Umeda K., Yoko-o T., Nakayama K., Suzuki T.-O., Jigami Y. Yeast 16:1261-1271(2000) [PubMed: 11015724] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329672 Genomic DNA. Translation: CAA20913.1.
PIR	T41318.
RefSeq	NP_587708.1. NM_001022703.1.
3D structure databases
ProteinModelPortal	O74878.
ModBase	Search...
Protein-protein interaction databases
STRING	O74878.
Protein family/group databases
CAZy	GT4. Glycosyltransferase Family 4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPCC330.08.1; SPCC330.08.1:pep; SPCC330.08.
GeneID	2539054.
GenomeReviews	Gene locus alg11 in contig CU329672_GR.
KEGG	spo:SPCC330.08.
NMPDR	fig|4896.1.peg.46.
Organism-specific databases
GeneDB_Spombe	SPCC330.08.
Phylogenomic databases
eggNOG	fuNOG07615.
GeneTree	EFGT00050000003720.
HOGENOM	HBG525304.
OMA	CRVGSYV.
OrthoDB	EOG4NS6M8.
Gene expression databases
ArrayExpress	O74878.
Family and domain databases
InterPro	IPR001296. Glyco_trans_1. [Graphical view]
KO	K03844.
Pfam	PF00534. Glycos_transf_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALG11_SCHPO

Accession

Primary (citable) accession number: O74878

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	November 1, 1998
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program