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Protein

GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase

Gene

alg11

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Required for N-linked oligosaccharide assembly. Has a role in the last step of the synthesis of the Man5GlcNAc(2)-PP-dolichol core oligosaccharide on the cytoplasmic face of the endoplasmic reticulum.1 Publication

Catalytic activityi

2 GDP-alpha-D-mannose + D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = 2 GDP + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol.

Pathway:iprotein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • alpha-1,2-mannosyltransferase activity Source: PomBase
  • GDP-Man:Man3GlcNAc2-PP-Dol alpha-1,2-mannosyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  • mannosylation Source: GOC
  • oligosaccharide-lipid intermediate biosynthetic process Source: PomBase
  • protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.131. 5613.
ReactomeiREACT_349845. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase (EC:2.4.1.131)
Alternative name(s):
Alpha-1,2-mannosyltransferase alg11
Asparagine-linked glycosylation protein 11
Galactomannan deficiency protein 3
Glycolipid 2-alpha-mannosyltransferase
Gene namesi
Name:alg11
Synonyms:gmd3
ORF Names:SPCC330.08
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC330.08.
PomBaseiSPCC330.08. alg11.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2525Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini26 – 471446LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: PomBase
  • endoplasmic reticulum membrane Source: PomBase
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 471471GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferasePRO_0000080278Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi97 – 971N-linked (GlcNAc...)Sequence Analysis
Glycosylationi236 – 2361N-linked (GlcNAc...)Sequence Analysis
Glycosylationi262 – 2621N-linked (GlcNAc...)Sequence Analysis
Glycosylationi390 – 3901N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiO74878.

Interactioni

Protein-protein interaction databases

BioGridi275627. 1 interaction.
MINTiMINT-4681498.

Structurei

3D structure databases

ProteinModelPortaliO74878.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0438.
HOGENOMiHOG000209670.
InParanoidiO74878.
KOiK03844.
OMAiGTVILAH.
OrthoDBiEOG7673KQ.
PhylomeDBiO74878.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O74878-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITTLAIALF IAVVAIHSHI RRKICKEIGI RLIKKIAPVK ASLYKQVGVE
60 70 80 90 100
PKLARTVGFF HPYCNAGGGG ERVLWTAVKS VQTEFPNVIS VVYTGDNVSK
110 120 130 140 150
AEILRRVKNT FEIDLDSSKI VFVYLKLRFL VSATTWHRFT LLGQSLGSMI
160 170 180 190 200
LGFEAIYRFA PDIFIDTMGY AFTFCVVKSF QNIPVGAYVH YPTISTDMLK
210 220 230 240 250
SLKQVSLLAK VKMAYWRWFA QLYSDAGSHA DYVMTNSSWT RNHIASLWGK
260 270 280 290 300
DIQLSVVFPP CNTSELEKID INRKREPTLL YLAQYRPEKN HENVLRSFAL
310 320 330 340 350
YFEQHPDSPA KLLLVGSVRG EEDMCFVNHL KTLATELNLQ SKVKFVVDAP
360 370 380 390 400
WPKVVEYLGT CSIGVNYMWN EHFGIGVVEY MAAGLIPVVN NSGGPKFDIV
410 420 430 440 450
IPWIGKPTGF HASTISEYAE AYHKALTLSP QEQLEMRINA RSACARFGEH
460 470
VFMRDFGNVF AKLLREDYSR T
Length:471
Mass (Da):53,278
Last modified:November 1, 1998 - v1
Checksum:i80D30A980D789366
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA20913.1.
PIRiT41318.
RefSeqiNP_587708.1. NM_001022703.2.

Genome annotation databases

EnsemblFungiiSPCC330.08.1; SPCC330.08.1:pep; SPCC330.08.
GeneIDi2539054.
KEGGispo:SPCC330.08.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA20913.1.
PIRiT41318.
RefSeqiNP_587708.1. NM_001022703.2.

3D structure databases

ProteinModelPortaliO74878.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275627. 1 interaction.
MINTiMINT-4681498.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Proteomic databases

MaxQBiO74878.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC330.08.1; SPCC330.08.1:pep; SPCC330.08.
GeneIDi2539054.
KEGGispo:SPCC330.08.

Organism-specific databases

EuPathDBiFungiDB:SPCC330.08.
PomBaseiSPCC330.08. alg11.

Phylogenomic databases

eggNOGiCOG0438.
HOGENOMiHOG000209670.
InParanoidiO74878.
KOiK03844.
OMAiGTVILAH.
OrthoDBiEOG7673KQ.
PhylomeDBiO74878.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.1.131. 5613.
ReactomeiREACT_349845. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

NextBioi20800228.
PROiO74878.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Schizosaccharomyces pombe gmd3(+)/alg11(+) is a functional homologue of Saccharomyces cerevisiae ALG11 which is involved in N-linked oligosaccharide synthesis."
    Umeda K., Yoko-o T., Nakayama K., Suzuki T.-O., Jigami Y.
    Yeast 16:1261-1271(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiALG11_SCHPO
AccessioniPrimary (citable) accession number: O74878
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: November 1, 1998
Last modified: July 22, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.