Reviewed, UniProtKB/Swiss-Prot O74878 (ALG11_SCHPO)

Names and origin

Protein names

Recommended name:
    Alpha-1,2-mannosyltransferase alg11
    EC=2.4.1.-
Alternative name(s):
    Asparagine-linked glycosylation protein 11
    Galactomannan deficiency protein 3

Gene names

Name:	alg11
Synonyms:	gmd3
ORF Names:	SPCC330.08

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Required for N-linked oligosaccharide assembly. Has a role in the last step of the synthesis of the Man5GlcNAc(2)-PP-dolichol core oligosaccharide on the cytoplasmic face of the endoplasmic reticulum. Ref.1
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 1 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid N-linked glycosylation Ref.1 Traceable author statement. Source: GeneDB_SPombe
Cellular component	alpha-1,6-mannosyltransferase complex Inferred by curator. Source: GeneDB_SPombe endoplasmic reticulum Inferred from direct assay. Source: GeneDB_SPombe integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-1,2-mannosyltransferase activity Inferred from genetic interaction. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	Alpha-1,2-mannosyltransferase alg11		PRO_0000080278
Regions
	Transmembrane	1 – 25	25	Signal-anchor for type II membrane protein Potential
	Topological domain	26 – 471	446	Lumenal Potential
Amino acid modifications
	Glycosylation	97	1	N-linked (GlcNAc...) Potential
	Glycosylation	236	1	N-linked (GlcNAc...) Potential
	Glycosylation	262	1	N-linked (GlcNAc...) Potential
	Glycosylation	390	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O74878-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 80D30A980D789366	FASTA	471	53,278
	10 20 30 40 50 60 MITTLAIALF IAVVAIHSHI RRKICKEIGI RLIKKIAPVK ASLYKQVGVE PKLARTVGFF 70 80 90 100 110 120 HPYCNAGGGG ERVLWTAVKS VQTEFPNVIS VVYTGDNVSK AEILRRVKNT FEIDLDSSKI 130 140 150 160 170 180 VFVYLKLRFL VSATTWHRFT LLGQSLGSMI LGFEAIYRFA PDIFIDTMGY AFTFCVVKSF 190 200 210 220 230 240 QNIPVGAYVH YPTISTDMLK SLKQVSLLAK VKMAYWRWFA QLYSDAGSHA DYVMTNSSWT 250 260 270 280 290 300 RNHIASLWGK DIQLSVVFPP CNTSELEKID INRKREPTLL YLAQYRPEKN HENVLRSFAL 310 320 330 340 350 360 YFEQHPDSPA KLLLVGSVRG EEDMCFVNHL KTLATELNLQ SKVKFVVDAP WPKVVEYLGT 370 380 390 400 410 420 CSIGVNYMWN EHFGIGVVEY MAAGLIPVVN NSGGPKFDIV IPWIGKPTGF HASTISEYAE 430 440 450 460 470 AYHKALTLSP QEQLEMRINA RSACARFGEH VFMRDFGNVF AKLLREDYSR T

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Schizosaccharomyces pombe gmd3(+)/alg11(+) is a functional homologue of Saccharomyces cerevisiae ALG11 which is involved in N-linked oligosaccharide synthesis." Umeda K., Yoko-o T., Nakayama K., Suzuki T.-O., Jigami Y. Yeast 16:1261-1271(2000) [PubMed: 11015724] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329672 Genomic DNA. Translation: CAA20913.1.
PIR	T41318.
RefSeq	NP_587708.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O74878.
Protein family/group databases
CAZy	GT4. Glycosyltransferase Family 4.
Genome annotation databases
GeneID	2539054.
GenomeReviews	Gene locus alg11 in contig CU329672_GR.
KEGG	spo:SPCC330.08.
NMPDR	fig|4896.1.peg.46.
Organism-specific databases
GeneDB_Spombe	SPCC330.08.
Phylogenomic databases
HOGENOM	HBG525304.
OMA	GCYVHYP.
OrthoDB	EOG9PG7HC.
Gene expression databases
ArrayExpress	O74878.
Family and domain databases
InterPro	IPR001296. Glyco_trans_1. [Graphical view]
Pfam	PF00534. Glycos_transf_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALG11_SCHPO

Accession

Primary (citable) accession number: O74878

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	November 1, 1998
Last modified:	December 15, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)