Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O74866 (RIFK_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Riboflavin kinase

EC=2.7.1.26
Alternative name(s):
ATP:riboflavin 5'-phosphotransferase
Flavin mononucleotide kinase 1
Flavokinase
Gene names
Name:fmn1
ORF Names:SPCC18.16c
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length163 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) coenzyme.

Catalytic activity

ATP + riboflavin = ADP + FMN.

Cofactor

Binds 1 zinc ion per subunit.

Pathway

Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.

Subunit structure

Monomer.

Sequence similarities

Belongs to the flavokinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 163163Riboflavin kinase
PRO_0000194151

Sites

Active site961Nucleophile Probable
Metal binding451Zinc

Secondary structure

........................... 163
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O74866 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: ADFD49D12FD7472F

FASTA16318,913
        10         20         30         40         50         60 
MTVNLEEKRP EIVGPEKVQS PYPIRFEGKV VHGFGRGSKE LGIPTANISE DAIQELLRYR 

        70         80         90        100        110        120 
DSGVYFGYAM VQKRVFPMVM SVGWNPYYKN KLRSAEVHLI ERQGEDFYEE IMRVIVLGYI 

       130        140        150        160 
RPELNYAGLD KLIEDIHTDI RVALNSMDRP SYSSYKKDPF FKV 

« Hide

References

« Hide 'large scale' references
[1]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"Crystal structure of Schizosaccharomyces pombe riboflavin kinase reveals a novel ATP and riboflavin-binding fold."
Bauer S., Kemter K., Bacher A., Huber R., Fischer M., Steinbacher S.
J. Mol. Biol. 326:1463-1473(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU329672 Genomic DNA. Translation: CAA21430.1.
PIRT41159.
RefSeqNP_588395.1. NM_001023386.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1N05X-ray2.10A1-163[»]
1N06X-ray2.00A/B1-163[»]
1N07X-ray2.45A/B1-163[»]
1N08X-ray1.60A/B1-163[»]
ProteinModelPortalO74866.
SMRO74866. Positions 8-162.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4681425.
STRING4896.SPCC18.16c-1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPCC18.16c.1; SPCC18.16c.1:pep; SPCC18.16c.
GeneID2539192.
KEGGspo:SPCC18.16c.

Organism-specific databases

PomBaseSPCC18.16c.

Phylogenomic databases

eggNOGCOG0196.
HOGENOMHOG000260803.
KOK00861.
OMASLPYFCR.
OrthoDBEOG7K3V05.
PhylomeDBO74866.

Enzyme and pathway databases

UniPathwayUPA00276; UER00406.

Family and domain databases

Gene3D2.40.30.30. 1 hit.
InterProIPR023468. Riboflavin_kinase.
IPR015865. Riboflavin_kinase_bac/euk.
IPR023465. Riboflavin_kinase_domain.
[Graphical view]
PANTHERPTHR22749. PTHR22749. 1 hit.
PfamPF01687. Flavokinase. 1 hit.
[Graphical view]
SMARTSM00904. Flavokinase. 1 hit.
[Graphical view]
SUPFAMSSF82114. SSF82114. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceO74866.
NextBio20800363.
PROO74866.

Entry information

Entry nameRIFK_SCHPO
AccessionPrimary (citable) accession number: O74866
Entry history
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: November 1, 1998
Last modified: April 16, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways