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Protein

Riboflavin kinase

Gene

fmn1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) coenzyme.

Catalytic activityi

ATP + riboflavin = ADP + FMN.

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi45 – 451Zinc
Active sitei96 – 961NucleophileCurated

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. riboflavin kinase activity Source: PomBase
  3. zinc ion binding Source: PomBase

GO - Biological processi

  1. FMN biosynthetic process Source: PomBase
  2. riboflavin biosynthetic process Source: InterPro
  3. riboflavin metabolic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Flavoprotein, FMN, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.1.26. 5613.
ReactomeiREACT_318965. Vitamin B2 (riboflavin) metabolism.
UniPathwayiUPA00276; UER00406.

Names & Taxonomyi

Protein namesi
Recommended name:
Riboflavin kinase (EC:2.7.1.26)
Alternative name(s):
ATP:riboflavin 5'-phosphotransferase
Flavin mononucleotide kinase 1
Flavokinase
Gene namesi
Name:fmn1
ORF Names:SPCC18.16c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC18.16c.
PomBaseiSPCC18.16c.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: PomBase
  2. mitochondrial inner membrane Source: PomBase
  3. nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 163163Riboflavin kinasePRO_0000194151Add
BLAST

Proteomic databases

MaxQBiO74866.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

MINTiMINT-4681425.
STRINGi4896.SPCC18.16c-1.

Structurei

Secondary structure

1
163
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi24 – 318Combined sources
Beta strandi34 – 363Combined sources
Helixi38 – 414Combined sources
Beta strandi46 – 483Combined sources
Helixi50 – 523Combined sources
Turni53 – 597Combined sources
Beta strandi62 – 7110Combined sources
Beta strandi74 – 8411Combined sources
Beta strandi93 – 997Combined sources
Beta strandi111 – 12111Combined sources
Helixi129 – 14719Combined sources
Helixi150 – 1534Combined sources
Helixi154 – 1574Combined sources
Helixi159 – 1613Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1N05X-ray2.10A1-163[»]
1N06X-ray2.00A/B1-163[»]
1N07X-ray2.45A/B1-163[»]
1N08X-ray1.60A/B1-163[»]
SMRiO74866. Positions 8-162.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO74866.

Family & Domainsi

Sequence similaritiesi

Belongs to the flavokinase family.Curated

Phylogenomic databases

eggNOGiCOG0196.
HOGENOMiHOG000260803.
InParanoidiO74866.
KOiK00861.
OMAiSLPYFCR.
OrthoDBiEOG7K3V05.
PhylomeDBiO74866.

Family and domain databases

Gene3Di2.40.30.30. 1 hit.
InterProiIPR023468. Riboflavin_kinase.
IPR015865. Riboflavin_kinase_bac/euk.
IPR023465. Riboflavin_kinase_domain.
[Graphical view]
PANTHERiPTHR22749. PTHR22749. 1 hit.
PfamiPF01687. Flavokinase. 1 hit.
[Graphical view]
SMARTiSM00904. Flavokinase. 1 hit.
[Graphical view]
SUPFAMiSSF82114. SSF82114. 1 hit.

Sequencei

Sequence statusi: Complete.

O74866-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVNLEEKRP EIVGPEKVQS PYPIRFEGKV VHGFGRGSKE LGIPTANISE
60 70 80 90 100
DAIQELLRYR DSGVYFGYAM VQKRVFPMVM SVGWNPYYKN KLRSAEVHLI
110 120 130 140 150
ERQGEDFYEE IMRVIVLGYI RPELNYAGLD KLIEDIHTDI RVALNSMDRP
160
SYSSYKKDPF FKV
Length:163
Mass (Da):18,913
Last modified:November 1, 1998 - v1
Checksum:iADFD49D12FD7472F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA21430.1.
PIRiT41159.
RefSeqiNP_588395.1. NM_001023386.2.

Genome annotation databases

EnsemblFungiiSPCC18.16c.1; SPCC18.16c.1:pep; SPCC18.16c.
GeneIDi2539192.
KEGGispo:SPCC18.16c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA21430.1.
PIRiT41159.
RefSeqiNP_588395.1. NM_001023386.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1N05X-ray2.10A1-163[»]
1N06X-ray2.00A/B1-163[»]
1N07X-ray2.45A/B1-163[»]
1N08X-ray1.60A/B1-163[»]
SMRiO74866. Positions 8-162.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4681425.
STRINGi4896.SPCC18.16c-1.

Proteomic databases

MaxQBiO74866.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC18.16c.1; SPCC18.16c.1:pep; SPCC18.16c.
GeneIDi2539192.
KEGGispo:SPCC18.16c.

Organism-specific databases

EuPathDBiFungiDB:SPCC18.16c.
PomBaseiSPCC18.16c.

Phylogenomic databases

eggNOGiCOG0196.
HOGENOMiHOG000260803.
InParanoidiO74866.
KOiK00861.
OMAiSLPYFCR.
OrthoDBiEOG7K3V05.
PhylomeDBiO74866.

Enzyme and pathway databases

UniPathwayiUPA00276; UER00406.
BRENDAi2.7.1.26. 5613.
ReactomeiREACT_318965. Vitamin B2 (riboflavin) metabolism.

Miscellaneous databases

EvolutionaryTraceiO74866.
NextBioi20800363.
PROiO74866.

Family and domain databases

Gene3Di2.40.30.30. 1 hit.
InterProiIPR023468. Riboflavin_kinase.
IPR015865. Riboflavin_kinase_bac/euk.
IPR023465. Riboflavin_kinase_domain.
[Graphical view]
PANTHERiPTHR22749. PTHR22749. 1 hit.
PfamiPF01687. Flavokinase. 1 hit.
[Graphical view]
SMARTiSM00904. Flavokinase. 1 hit.
[Graphical view]
SUPFAMiSSF82114. SSF82114. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Crystal structure of Schizosaccharomyces pombe riboflavin kinase reveals a novel ATP and riboflavin-binding fold."
    Bauer S., Kemter K., Bacher A., Huber R., Fischer M., Steinbacher S.
    J. Mol. Biol. 326:1463-1473(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

Entry informationi

Entry nameiRIFK_SCHPO
AccessioniPrimary (citable) accession number: O74866
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: November 1, 1998
Last modified: April 29, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.