Riboflavin kinase - Schizosaccharomyces pombe (Fission yeast)

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Reviewed, UniProtKB/Swiss-Prot O74866 (RIFK_SCHPO)

Last modified June 16, 2009. Version 50. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Riboflavin kinase
    EC=2.7.1.26
Alternative name(s):
    Flavin mononucleotide kinase 1
    ATP:riboflavin 5'-phosphotransferase
    Flavokinase

Gene names

Name:	fmn1
ORF Names:	SPCC18.16c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

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Protein attributes

Sequence length	163 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) coenzyme.
Catalytic activity	ATP + riboflavin = ADP + FMN.
Cofactor	Binds 1 zinc ion per subunit.
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.
Subunit structure	Monomer.
Sequence similarities	Belongs to the flavokinase family.

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Ontologies

Keywords
Ligand	ATP-binding FMN Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe nucleus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 163

163

Riboflavin kinase

PRO_0000194151

Sites

Active site

Nucleophile Probable

Metal binding

Zinc

Secondary structure

163

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

O74866-1 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: ADFD49D12FD7472F
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FASTA

163

18,913

        10         20         30         40         50         60 
MTVNLEEKRP EIVGPEKVQS PYPIRFEGKV VHGFGRGSKE LGIPTANISE DAIQELLRYR 

        70         80         90        100        110        120 
DSGVYFGYAM VQKRVFPMVM SVGWNPYYKN KLRSAEVHLI ERQGEDFYEE IMRVIVLGYI 

       130        140        150        160 
RPELNYAGLD KLIEDIHTDI RVALNSMDRP SYSSYKKDPF FKV

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[2]	"Crystal structure of Schizosaccharomyces pombe riboflavin kinase reveals a novel ATP and riboflavin-binding fold." Bauer S., Kemter K., Bacher A., Huber R., Fischer M., Steinbacher S. J. Mol. Biol. 326:1463-1473(2003) [PubMed: 12595258] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

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Cross-references

Sequence databases

CU329672 Genomic DNA. Translation: CAA21430.1.

PIR

T41159.

RefSeq

NP_588395.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1N05	X-ray	2.10	A	1-163	[»]
1N06	X-ray	2.00	A/B	1-163	[»]
1N07	X-ray	2.45	A/B	1-163	[»]
1N08	X-ray	1.60	A/B	1-163	[»]

ModBase

Search...

Genome annotation databases

GeneID

2539192.

KEGG

spo:SPCC18.16c.

NMPDR

fig|4896.1.peg.733.

Organism-specific databases

GeneDB_Spombe

SPCC18.16c.

Phylogenomic databases

OMA

O74866. METHVIH.

Enzyme and pathway databases

BRENDA

2.7.1.26. 653.

Gene expression databases

ArrayExpress

O74866.

Family and domain databases

InterPro

IPR015865. Riboflavin_kinase.
[Graphical view]

Gene3D

G3DSA:2.40.30.30. Riboflavin_kinase. 1 hit.

PANTHER

PTHR22749. Riboflavin_kinase. 1 hit.

Pfam

PF01687. Flavokinase. 1 hit.
[Graphical view]

ProDom

PD003662. FAD_Synth. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

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Entry information

Entry name

RIFK_SCHPO

Accession

Primary (citable) accession number: O74866

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	November 1, 1998
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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