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O74866

- RIFK_SCHPO

UniProt

O74866 - RIFK_SCHPO

Protein

Riboflavin kinase

Gene

fmn1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) coenzyme.

    Catalytic activityi

    ATP + riboflavin = ADP + FMN.

    Cofactori

    Binds 1 zinc ion per subunit.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi45 – 451Zinc
    Active sitei96 – 961NucleophileCurated

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. riboflavin kinase activity Source: PomBase
    3. zinc ion binding Source: PomBase

    GO - Biological processi

    1. FMN biosynthetic process Source: PomBase
    2. riboflavin biosynthetic process Source: InterPro
    3. riboflavin metabolic process Source: PomBase

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Flavoprotein, FMN, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00276; UER00406.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Riboflavin kinase (EC:2.7.1.26)
    Alternative name(s):
    ATP:riboflavin 5'-phosphotransferase
    Flavin mononucleotide kinase 1
    Flavokinase
    Gene namesi
    Name:fmn1
    ORF Names:SPCC18.16c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome III

    Organism-specific databases

    PomBaseiSPCC18.16c.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: PomBase
    2. mitochondrial inner membrane Source: PomBase
    3. nucleus Source: PomBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 163163Riboflavin kinasePRO_0000194151Add
    BLAST

    Proteomic databases

    MaxQBiO74866.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    MINTiMINT-4681425.
    STRINGi4896.SPCC18.16c-1.

    Structurei

    Secondary structure

    1
    163
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi24 – 318
    Beta strandi34 – 363
    Helixi38 – 414
    Beta strandi46 – 483
    Helixi50 – 523
    Turni53 – 597
    Beta strandi62 – 7110
    Beta strandi74 – 8411
    Beta strandi93 – 997
    Beta strandi111 – 12111
    Helixi129 – 14719
    Helixi150 – 1534
    Helixi154 – 1574
    Helixi159 – 1613

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1N05X-ray2.10A1-163[»]
    1N06X-ray2.00A/B1-163[»]
    1N07X-ray2.45A/B1-163[»]
    1N08X-ray1.60A/B1-163[»]
    ProteinModelPortaliO74866.
    SMRiO74866. Positions 8-162.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO74866.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the flavokinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0196.
    HOGENOMiHOG000260803.
    KOiK00861.
    OMAiSLPYFCR.
    OrthoDBiEOG7K3V05.
    PhylomeDBiO74866.

    Family and domain databases

    Gene3Di2.40.30.30. 1 hit.
    InterProiIPR023468. Riboflavin_kinase.
    IPR015865. Riboflavin_kinase_bac/euk.
    IPR023465. Riboflavin_kinase_domain.
    [Graphical view]
    PANTHERiPTHR22749. PTHR22749. 1 hit.
    PfamiPF01687. Flavokinase. 1 hit.
    [Graphical view]
    SMARTiSM00904. Flavokinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF82114. SSF82114. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O74866-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTVNLEEKRP EIVGPEKVQS PYPIRFEGKV VHGFGRGSKE LGIPTANISE    50
    DAIQELLRYR DSGVYFGYAM VQKRVFPMVM SVGWNPYYKN KLRSAEVHLI 100
    ERQGEDFYEE IMRVIVLGYI RPELNYAGLD KLIEDIHTDI RVALNSMDRP 150
    SYSSYKKDPF FKV 163
    Length:163
    Mass (Da):18,913
    Last modified:November 1, 1998 - v1
    Checksum:iADFD49D12FD7472F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329672 Genomic DNA. Translation: CAA21430.1.
    PIRiT41159.
    RefSeqiNP_588395.1. NM_001023386.2.

    Genome annotation databases

    EnsemblFungiiSPCC18.16c.1; SPCC18.16c.1:pep; SPCC18.16c.
    GeneIDi2539192.
    KEGGispo:SPCC18.16c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329672 Genomic DNA. Translation: CAA21430.1 .
    PIRi T41159.
    RefSeqi NP_588395.1. NM_001023386.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1N05 X-ray 2.10 A 1-163 [» ]
    1N06 X-ray 2.00 A/B 1-163 [» ]
    1N07 X-ray 2.45 A/B 1-163 [» ]
    1N08 X-ray 1.60 A/B 1-163 [» ]
    ProteinModelPortali O74866.
    SMRi O74866. Positions 8-162.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4681425.
    STRINGi 4896.SPCC18.16c-1.

    Proteomic databases

    MaxQBi O74866.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPCC18.16c.1 ; SPCC18.16c.1:pep ; SPCC18.16c .
    GeneIDi 2539192.
    KEGGi spo:SPCC18.16c.

    Organism-specific databases

    PomBasei SPCC18.16c.

    Phylogenomic databases

    eggNOGi COG0196.
    HOGENOMi HOG000260803.
    KOi K00861.
    OMAi SLPYFCR.
    OrthoDBi EOG7K3V05.
    PhylomeDBi O74866.

    Enzyme and pathway databases

    UniPathwayi UPA00276 ; UER00406 .

    Miscellaneous databases

    EvolutionaryTracei O74866.
    NextBioi 20800363.
    PROi O74866.

    Family and domain databases

    Gene3Di 2.40.30.30. 1 hit.
    InterProi IPR023468. Riboflavin_kinase.
    IPR015865. Riboflavin_kinase_bac/euk.
    IPR023465. Riboflavin_kinase_domain.
    [Graphical view ]
    PANTHERi PTHR22749. PTHR22749. 1 hit.
    Pfami PF01687. Flavokinase. 1 hit.
    [Graphical view ]
    SMARTi SM00904. Flavokinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF82114. SSF82114. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "Crystal structure of Schizosaccharomyces pombe riboflavin kinase reveals a novel ATP and riboflavin-binding fold."
      Bauer S., Kemter K., Bacher A., Huber R., Fischer M., Steinbacher S.
      J. Mol. Biol. 326:1463-1473(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

    Entry informationi

    Entry nameiRIFK_SCHPO
    AccessioniPrimary (citable) accession number: O74866
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 31, 2004
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3