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Protein

Riboflavin kinase

Gene

fmn1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) coenzyme.

Catalytic activityi

ATP + riboflavin = ADP + FMN.

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Pathwayi: FMN biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes FMN from riboflavin (ATP route).
Proteins known to be involved in this subpathway in this organism are:
  1. Riboflavin kinase (fmn1)
This subpathway is part of the pathway FMN biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes FMN from riboflavin (ATP route), the pathway FMN biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi45Zinc1
Active sitei96NucleophileCurated1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • riboflavin kinase activity Source: PomBase
  • zinc ion binding Source: PomBase

GO - Biological processi

  • FMN biosynthetic process Source: PomBase
  • riboflavin biosynthetic process Source: InterPro
  • riboflavin metabolic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Flavoprotein, FMN, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.1.26. 5613.
ReactomeiR-SPO-196843. Vitamin B2 (riboflavin) metabolism.
UniPathwayiUPA00276; UER00406.

Names & Taxonomyi

Protein namesi
Recommended name:
Riboflavin kinase (EC:2.7.1.26)
Alternative name(s):
ATP:riboflavin 5'-phosphotransferase
Flavin mononucleotide kinase 1
Flavokinase
Gene namesi
Name:fmn1
ORF Names:SPCC18.16c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC18.16c.
PomBaseiSPCC18.16c. fmn1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • mitochondrial inner membrane Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001941511 – 163Riboflavin kinaseAdd BLAST163

Proteomic databases

MaxQBiO74866.
PRIDEiO74866.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

MINTiMINT-4681425.

Structurei

Secondary structure

1163
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi24 – 31Combined sources8
Beta strandi34 – 36Combined sources3
Helixi38 – 41Combined sources4
Beta strandi46 – 48Combined sources3
Helixi50 – 52Combined sources3
Turni53 – 59Combined sources7
Beta strandi62 – 71Combined sources10
Beta strandi74 – 84Combined sources11
Beta strandi93 – 99Combined sources7
Beta strandi111 – 121Combined sources11
Helixi129 – 147Combined sources19
Helixi150 – 153Combined sources4
Helixi154 – 157Combined sources4
Helixi159 – 161Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1N05X-ray2.10A1-163[»]
1N06X-ray2.00A/B1-163[»]
1N07X-ray2.45A/B1-163[»]
1N08X-ray1.60A/B1-163[»]
ProteinModelPortaliO74866.
SMRiO74866.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO74866.

Family & Domainsi

Sequence similaritiesi

Belongs to the flavokinase family.Curated

Phylogenomic databases

HOGENOMiHOG000260803.
InParanoidiO74866.
KOiK00861.
OMAiSLPYFCR.
OrthoDBiEOG092C5F1Q.
PhylomeDBiO74866.

Family and domain databases

Gene3Di2.40.30.30. 1 hit.
InterProiIPR023468. Riboflavin_kinase.
IPR015865. Riboflavin_kinase_bac/euk.
IPR023465. Riboflavin_kinase_domain.
[Graphical view]
PANTHERiPTHR22749. PTHR22749. 1 hit.
PfamiPF01687. Flavokinase. 1 hit.
[Graphical view]
SMARTiSM00904. Flavokinase. 1 hit.
[Graphical view]
SUPFAMiSSF82114. SSF82114. 1 hit.

Sequencei

Sequence statusi: Complete.

O74866-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVNLEEKRP EIVGPEKVQS PYPIRFEGKV VHGFGRGSKE LGIPTANISE
60 70 80 90 100
DAIQELLRYR DSGVYFGYAM VQKRVFPMVM SVGWNPYYKN KLRSAEVHLI
110 120 130 140 150
ERQGEDFYEE IMRVIVLGYI RPELNYAGLD KLIEDIHTDI RVALNSMDRP
160
SYSSYKKDPF FKV
Length:163
Mass (Da):18,913
Last modified:November 1, 1998 - v1
Checksum:iADFD49D12FD7472F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA21430.1.
PIRiT41159.
RefSeqiNP_588395.1. NM_001023386.2.

Genome annotation databases

EnsemblFungiiSPCC18.16c.1; SPCC18.16c.1:pep; SPCC18.16c.
GeneIDi2539192.
KEGGispo:SPCC18.16c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA21430.1.
PIRiT41159.
RefSeqiNP_588395.1. NM_001023386.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1N05X-ray2.10A1-163[»]
1N06X-ray2.00A/B1-163[»]
1N07X-ray2.45A/B1-163[»]
1N08X-ray1.60A/B1-163[»]
ProteinModelPortaliO74866.
SMRiO74866.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4681425.

Proteomic databases

MaxQBiO74866.
PRIDEiO74866.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC18.16c.1; SPCC18.16c.1:pep; SPCC18.16c.
GeneIDi2539192.
KEGGispo:SPCC18.16c.

Organism-specific databases

EuPathDBiFungiDB:SPCC18.16c.
PomBaseiSPCC18.16c. fmn1.

Phylogenomic databases

HOGENOMiHOG000260803.
InParanoidiO74866.
KOiK00861.
OMAiSLPYFCR.
OrthoDBiEOG092C5F1Q.
PhylomeDBiO74866.

Enzyme and pathway databases

UniPathwayiUPA00276; UER00406.
BRENDAi2.7.1.26. 5613.
ReactomeiR-SPO-196843. Vitamin B2 (riboflavin) metabolism.

Miscellaneous databases

EvolutionaryTraceiO74866.
PROiO74866.

Family and domain databases

Gene3Di2.40.30.30. 1 hit.
InterProiIPR023468. Riboflavin_kinase.
IPR015865. Riboflavin_kinase_bac/euk.
IPR023465. Riboflavin_kinase_domain.
[Graphical view]
PANTHERiPTHR22749. PTHR22749. 1 hit.
PfamiPF01687. Flavokinase. 1 hit.
[Graphical view]
SMARTiSM00904. Flavokinase. 1 hit.
[Graphical view]
SUPFAMiSSF82114. SSF82114. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRIFK_SCHPO
AccessioniPrimary (citable) accession number: O74866
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.