Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit trm82

Gene

trm82

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. In the complex, it is required to stabilize and induce conformational changes of the catalytic subunit.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit trm82UniRule annotation
Alternative name(s):
Transfer RNA methyltransferase 82UniRule annotation
Gene namesi
Name:trm82
ORF Names:SPCC18.13
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC18.13.
PomBaseiSPCC18.13. trm82.

Subcellular locationi

  • Nucleus UniRule annotation

GO - Cellular componenti

  • cytosol Source: PomBase
  • nucleus Source: PomBase
  • protein complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 421421tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit trm82PRO_0000051294Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei413 – 4131Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO74863.

PTM databases

iPTMnetiO74863.

Interactioni

Subunit structurei

Forms a heterodimer with the catalytic subunit trm8.UniRule annotation

Protein-protein interaction databases

BioGridi275816. 74 interactions.
MINTiMINT-4681396.

Structurei

3D structure databases

ProteinModelPortaliO74863.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati60 – 9940WD 1Add
BLAST
Repeati176 – 22247WD 2Add
BLAST
Repeati224 – 26441WD 3Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat TRM82 family.UniRule annotation
Contains 3 WD repeats.UniRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

InParanoidiO74863.
KOiK15443.
OrthoDBiEOG092C34WZ.
PhylomeDBiO74863.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
HAMAPiMF_03056. TRM82. 1 hit.
InterProiIPR028884. Trm82.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00320. WD40. 2 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O74863-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEQRVFKHP CQFLTWNSKH NYIVCCSGPY LLGFSCSTGE KIFEHCYRDN
60 70 80 90 100
INEKHREAAA YGEAIRQVAF SKDYSRMATV SEDKCLRLWD STQPDKIELL
110 120 130 140 150
YQKNIPKRCA DLCFAGSNEI VFGDKFGDVY CVDENWFTTS EVTEEKKSNV
160 170 180 190 200
VEGKQEPVNN DTLKDSKLQK LEPIMGHVSI LTQLIVAQNP QNSKEEIIIT
210 220 230 240 250
SDKDEHIRIS RFPNAFVIEG FCLGHEDFVS RMSLYDNRTL ISGGGDNHVF
260 270 280 290 300
VWDLENFKCL DAFDLRSAFS TYLSLNQPMV VSVILPIFKR QLVAFACEGM
310 320 330 340 350
AGLIFAKVTP EKRLLFHSAL KLSGPVLDAV LLDTDTDQIL ISLDSSFTFG
360 370 380 390 400
ACFECVKFDE GNAAVLTKPD VIKRIESDGL ISTEKPFCPL AQIHTLRKNH
410 420
SKFIRSVETG TSSPSVESKD N
Length:421
Mass (Da):47,498
Last modified:November 1, 1998 - v1
Checksum:i7B78AF95ABD7FDBA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA21427.1.
PIRiT41156.
RefSeqiNP_588392.1. NM_001023383.2.

Genome annotation databases

EnsemblFungiiSPCC18.13.1; SPCC18.13.1:pep; SPCC18.13.
GeneIDi2539246.
KEGGispo:SPCC18.13.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA21427.1.
PIRiT41156.
RefSeqiNP_588392.1. NM_001023383.2.

3D structure databases

ProteinModelPortaliO74863.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275816. 74 interactions.
MINTiMINT-4681396.

PTM databases

iPTMnetiO74863.

Proteomic databases

MaxQBiO74863.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC18.13.1; SPCC18.13.1:pep; SPCC18.13.
GeneIDi2539246.
KEGGispo:SPCC18.13.

Organism-specific databases

EuPathDBiFungiDB:SPCC18.13.
PomBaseiSPCC18.13. trm82.

Phylogenomic databases

InParanoidiO74863.
KOiK15443.
OrthoDBiEOG092C34WZ.
PhylomeDBiO74863.

Enzyme and pathway databases

UniPathwayiUPA00989.

Miscellaneous databases

PROiO74863.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
HAMAPiMF_03056. TRM82. 1 hit.
InterProiIPR028884. Trm82.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00320. WD40. 2 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRM82_SCHPO
AccessioniPrimary (citable) accession number: O74863
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2003
Last sequence update: November 1, 1998
Last modified: September 7, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.