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Protein

Aprataxin-like protein

Gene

hnt3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined (By similarity).By similarity

GO - Molecular functioni

  • DNA 5'-adenosine monophosphate hydrolase activity Source: PomBase
  • mismatched DNA binding Source: PomBase
  • single-strand break-containing DNA binding Source: PomBase
  • single-stranded DNA binding Source: PomBase
  • zinc ion binding Source: PomBase

GO - Biological processi

  • DNA repair Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aprataxin-like protein (EC:3.-.-.-)
Alternative name(s):
Hit family protein 3
Gene namesi
Name:hnt3
ORF Names:SPCC18.09c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC18.09c.
PomBaseiSPCC18.09c. hnt3.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003146501 – 232Aprataxin-like proteinAdd BLAST232

Proteomic databases

MaxQBiO74859.
PRIDEiO74859.

Interactioni

Protein-protein interaction databases

BioGridi275769. 3 interactors.
MINTiMINT-4681359.

Structurei

Secondary structure

1232
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi34 – 38Combined sources5
Helixi39 – 43Combined sources5
Helixi45 – 47Combined sources3
Beta strandi51 – 54Combined sources4
Beta strandi56 – 62Combined sources7
Beta strandi67 – 76Combined sources10
Turni79 – 83Combined sources5
Helixi86 – 92Combined sources7
Helixi94 – 105Combined sources12
Turni106 – 108Combined sources3
Helixi109 – 119Combined sources11
Helixi126 – 130Combined sources5
Beta strandi133 – 140Combined sources8
Beta strandi142 – 145Combined sources4
Beta strandi148 – 153Combined sources6
Helixi163 – 169Combined sources7
Beta strandi174 – 176Combined sources3
Helixi181 – 183Combined sources3
Helixi190 – 192Combined sources3
Helixi193 – 195Combined sources3
Turni201 – 203Combined sources3
Beta strandi206 – 208Combined sources3
Helixi211 – 229Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SP4X-ray1.80A/B33-232[»]
3SPDX-ray1.91A/B/C/D33-232[»]
3SPLX-ray2.10A/B/C/D33-232[»]
3SZQX-ray2.35A31-232[»]
4XBAX-ray1.50A/B33-232[»]
4YKLX-ray2.25B33-232[»]
ProteinModelPortaliO74859.
SMRiO74859.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 160HITAdd BLAST123

Sequence similaritiesi

Contains 1 HIT domain.Curated

Phylogenomic databases

HOGENOMiHOG000167639.
InParanoidiO74859.
KOiK10863.
OMAiHIHVITQ.
OrthoDBiEOG092C3DRA.
PhylomeDBiO74859.

Family and domain databases

Gene3Di3.30.428.10. 1 hit.
InterProiIPR001310. Histidine_triad_HIT.
IPR011146. HIT-like.
IPR032566. Znf-C2HE.
[Graphical view]
PfamiPF01230. HIT. 1 hit.
PF16278. zf-C2HE. 1 hit.
[Graphical view]
SUPFAMiSSF54197. SSF54197. 1 hit.

Sequencei

Sequence statusi: Complete.

O74859-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVHKTNDAF KVLMNSAKEP IVEDIPKKYR KQSFRDNLKV YIESPESYKN
60 70 80 90 100
VIYYDDDVVL VRDMFPKSKM HLLLMTRDPH LTHVHPLEIM MKHRSLVEKL
110 120 130 140 150
VSYVQGDLSG LIFDEARNCL SQQLTNEALC NYIKVGFHAG PSMNNLHLHI
160 170 180 190 200
MTLDHVSPSL KNSAHYISFT SPFFVKIDTP TSNLPTRGTL TSLFQEDLKC
210 220 230
WRCGETFGRH FTKLKAHLQE EYDDWLDKSV SM
Length:232
Mass (Da):26,922
Last modified:November 1, 1998 - v1
Checksum:i7B448C953ED13CFB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA21423.1.
PIRiT41152.
RefSeqiNP_588388.1. NM_001023379.2.

Genome annotation databases

EnsemblFungiiSPCC18.09c.1; SPCC18.09c.1:pep; SPCC18.09c.
GeneIDi2539198.
KEGGispo:SPCC18.09c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA21423.1.
PIRiT41152.
RefSeqiNP_588388.1. NM_001023379.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SP4X-ray1.80A/B33-232[»]
3SPDX-ray1.91A/B/C/D33-232[»]
3SPLX-ray2.10A/B/C/D33-232[»]
3SZQX-ray2.35A31-232[»]
4XBAX-ray1.50A/B33-232[»]
4YKLX-ray2.25B33-232[»]
ProteinModelPortaliO74859.
SMRiO74859.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275769. 3 interactors.
MINTiMINT-4681359.

Proteomic databases

MaxQBiO74859.
PRIDEiO74859.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC18.09c.1; SPCC18.09c.1:pep; SPCC18.09c.
GeneIDi2539198.
KEGGispo:SPCC18.09c.

Organism-specific databases

EuPathDBiFungiDB:SPCC18.09c.
PomBaseiSPCC18.09c. hnt3.

Phylogenomic databases

HOGENOMiHOG000167639.
InParanoidiO74859.
KOiK10863.
OMAiHIHVITQ.
OrthoDBiEOG092C3DRA.
PhylomeDBiO74859.

Miscellaneous databases

PROiO74859.

Family and domain databases

Gene3Di3.30.428.10. 1 hit.
InterProiIPR001310. Histidine_triad_HIT.
IPR011146. HIT-like.
IPR032566. Znf-C2HE.
[Graphical view]
PfamiPF01230. HIT. 1 hit.
PF16278. zf-C2HE. 1 hit.
[Graphical view]
SUPFAMiSSF54197. SSF54197. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAPTX_SCHPO
AccessioniPrimary (citable) accession number: O74859
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.