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Protein

Ribosome assembly protein 4

Gene

SPCC18.05c

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Involved in ribosome biogenesis. Required for processing and efficient intra-nuclear transport of pre-60S ribosomal subunits. Interacts with the AAA-ATPase Midasin, which is essential for the ATP-dependent dissociation of a group of nonribosomal factors from the pre-60S particle.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome assembly protein 4By similarity
Alternative name(s):
Notchless protein homolog 1By similarity
Ribosome biogenesis factor rsa4By similarity
Gene namesi
ORF Names:SPCC18.05cImported
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC18.05c.
PomBaseiSPCC18.05c.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: PomBase
  • nucleus Source: PomBase
  • protein complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 502502Ribosome assembly protein 4PRO_0000316570Add
BLAST

Proteomic databases

MaxQBiO74855.

Interactioni

Subunit structurei

Associates with the pre-60S ribosomal particle. Interacts with Midasin.By similarity

Protein-protein interaction databases

BioGridi275946. 2 interactions.
MINTiMINT-4681320.

Structurei

3D structure databases

ProteinModelPortaliO74855.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati132 – 17241WD 1Sequence analysisAdd
BLAST
Repeati175 – 21440WD 2Sequence analysisAdd
BLAST
Repeati218 – 26447WD 3Sequence analysisAdd
BLAST
Repeati267 – 30741WD 4Sequence analysisAdd
BLAST
Repeati344 – 38340WD 5Sequence analysisAdd
BLAST
Repeati387 – 42640WD 6Sequence analysisAdd
BLAST
Repeati429 – 46840WD 7Sequence analysisAdd
BLAST
Repeati471 – 50232WD 8Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the NLE1/RSA4 family.Curated
Contains 8 WD repeats.Sequence analysis

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

HOGENOMiHOG000091641.
InParanoidiO74855.
KOiK14855.
OMAiHINCLAW.
OrthoDBiEOG73Z348.
PhylomeDBiO74855.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR012972. NLE.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08154. NLE. 1 hit.
PF00400. WD40. 8 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 8 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 5 hits.
PS50082. WD_REPEATS_2. 7 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O74855-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLLPPKSK RQKKESLNPT TIEIPEKFPL VNVQFRASDD SNELASLLVP
60 70 80 90 100
GNSSVRQLEA LLNQLLENSD DPVPYNFALH HEDETIEIQD NLYTSVFHNG
110 120 130 140 150
LMKTEDHLTL LYTPQAVFRV RAVTRCTASM NGHDGTIISA QFSPSTSSRL
160 170 180 190 200
VTGSGDFTAR LWDCDTQTPI ATMKGHTNWV SCVAWAPDAS IIATGSMDNT
210 220 230 240 250
IRFWDPKKGS PIGDALRRHT KPIMALCWQP LHLAPDSGPY LLASGSKDNT
260 270 280 290 300
VRIWNVKLRT LLFTLSGHTA PITCVRWGGQ NWIYSSSYDK TIRIWDAKDG
310 320 330 340 350
KCLHILKGHA ARVNHLSLST EHVLRSGAYD HTDFKPKSFS DERRKAKERY
360 370 380 390 400
EACLKQSGER LVSASDDLQL ILWDPQKSTK PITKMHGHQK VVNHASFSPD
410 420 430 440 450
GRCIATASFD SSVRLWDGKT GKFLATLRGH VAAVYQCAWS TDSRLLVSSS
460 470 480 490 500
QDTTLKVWDV RSKKMKFDLP GHEDQVFAVD WSPDGQRVAS GGADKAVRIW

SH
Length:502
Mass (Da):56,003
Last modified:November 1, 1998 - v1
Checksum:i5773208C7DEEB23A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA21419.1.
PIRiT41148.
RefSeqiNP_588384.1. NM_001023375.2.

Genome annotation databases

EnsemblFungiiSPCC18.05c.1; SPCC18.05c.1:pep; SPCC18.05c.
GeneIDi2539380.
KEGGispo:SPCC18.05c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA21419.1.
PIRiT41148.
RefSeqiNP_588384.1. NM_001023375.2.

3D structure databases

ProteinModelPortaliO74855.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275946. 2 interactions.
MINTiMINT-4681320.

Proteomic databases

MaxQBiO74855.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC18.05c.1; SPCC18.05c.1:pep; SPCC18.05c.
GeneIDi2539380.
KEGGispo:SPCC18.05c.

Organism-specific databases

EuPathDBiFungiDB:SPCC18.05c.
PomBaseiSPCC18.05c.

Phylogenomic databases

HOGENOMiHOG000091641.
InParanoidiO74855.
KOiK14855.
OMAiHINCLAW.
OrthoDBiEOG73Z348.
PhylomeDBiO74855.

Miscellaneous databases

NextBioi20800545.
PROiO74855.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR012972. NLE.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08154. NLE. 1 hit.
PF00400. WD40. 8 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 8 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 5 hits.
PS50082. WD_REPEATS_2. 7 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNLE1_SCHPO
AccessioniPrimary (citable) accession number: O74855
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 1, 1998
Last modified: May 11, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.