Reviewed, UniProtKB/Swiss-Prot O74850 (DGA1_SCHPO)

Names and origin

Protein names

Recommended name:
    Diacylglycerol O-acyltransferase 1
    EC=2.3.1.20
Alternative name(s):
    Diglyceride acyltransferase

Gene names

Name:	dga1
ORF Names:	SPCC1235.15, SPCC548.01

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Cells defective in triacylglycerol synthesis undergo apoptosis upon entry into the stationary phase. Generation of reactive oxygen species (ROS) is essential to lipoapoptosis. Ref.2
Catalytic activity	Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol. Ref.2
Pathway	Glycerolipid metabolism; triacylglycerol biosynthesis.
Subcellular location	Lipid droplet. Membrane; Single-pass membrane protein. Note: Localized to lipid particles. Ref.2
Sequence similarities	Belongs to the diacylglycerol acyltransferase family.

Ontologies

Keywords
Cellular component	Lipid droplet Membrane
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diacylglycerol metabolic process Ref.2 Inferred by curator. Source: GeneDB_SPombe lipid storage Ref.2 Inferred by curator. Source: GeneDB_SPombe triglyceride biosynthetic process Ref.2 Inferred from direct assay. Source: GeneDB_SPombe
Cellular component	endoplasmic reticulum Inferred from direct assay. Source: GeneDB_SPombe integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell lipid particle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	diacylglycerol O-acyltransferase activity Ref.2 Inferred from direct assay. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Diacylglycerol O-acyltransferase 1		PRO_0000176219
Regions
	Transmembrane	50 – 70	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	O74850-1 [UniParc]. Last modified May 10, 2004. Version 3. Checksum: 4360E92C3BA497AA	FASTA	345	39,486
	10 20 30 40 50 60 MSEETSIPGI IASTPPISKD SRRNVSHWLQ ALAVFLHSVS LTLTASWYTV LWAFLPFWPF 70 80 90 100 110 120 LIVYLIWLIY DDGFVTGKDR QKRWLRNAPP YRWFCHYFPI RLHKTTELDS EKNYIFGYHP 130 140 150 160 170 180 HGIISLGAFG GFASEGADFS KLFPGINVSV LTLNSNFYVP VYRDYLMALN INSVSKKSCV 190 200 210 220 230 240 SILSRKPGDS VLIVIGGAQE SLLSRPGQNN LVLKKRFGFV KLAFLTGSSL VPCFAFGESD 250 260 270 280 290 300 IFEQVDNNPR TRIYKFQEIV KKIAGFTVPF FYGRGLLNKT FGLMPWRKPI NIVVGEPIDV 310 320 330 340 PKKSHPTNQE IYEVHEEYIR RLEGLWNKYK DVFLPNRISE LKLSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[2]	"Schizosaccharomyces pombe cells deficient in triacylglycerols synthesis undergo apoptosis upon entry into the stationary phase." Zhang Q., Chieu H.K., Low C.P., Zhang S., Heng C.K., Yang H. J. Biol. Chem. 278:47145-47155(2003) [PubMed: 12963726] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329672 Genomic DNA. Translation: CAE54965.1.
RefSeq	XP_001713160.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O74850.
Genome annotation databases
GeneID	5802702.
GenomeReviews	Gene locus dga1 in contig CU329672_GR.
KEGG	spo:SPCC1235.15.
Organism-specific databases
GeneDB_Spombe	SPCC1235.15.
Enzyme and pathway databases
BRENDA	2.3.1.20. 653.
Gene expression databases
ArrayExpress	O74850.
Family and domain databases
InterPro	IPR007130. DAGAT. [Graphical view]
PANTHER	PTHR12317. DAGAT. 1 hit.
Pfam	PF03982. DAGAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DGA1_SCHPO

Accession

Primary (citable) accession number: O74850
Secondary accession number(s): Q9P3V1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 13, 2002
Last sequence update:	May 10, 2004
Last modified:	October 13, 2009
This is version 56 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)