ID   DNLI4_SCHPO             Reviewed;         923 AA.
AC   O74833;
DT   07-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 2.
DT   16-JUN-2009, entry version 51.
DE   RecName: Full=DNA ligase 4;
DE            EC=6.5.1.1;
DE   AltName: Full=DNA ligase IV;
DE   AltName: Full=Polydeoxyribonucleotide synthase [ATP] 4;
GN   Name=lig4; ORFNames=SPCC1183.05c;
OS   Schizosaccharomyces pombe (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales;
OC   Schizosaccharomycetaceae; Schizosaccharomyces.
OX   NCBI_TaxID=4896;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 38366 / 972;
RX   MEDLINE=21848401; PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M.,
RA   Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A.,
RA   Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G.,
RA   Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K.,
RA   James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J.,
RA   Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C.,
RA   Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E.,
RA   Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S.,
RA   Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K.,
RA   Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S.,
RA   Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B.,
RA   Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S.,
RA   Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D.,
RA   Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R.,
RA   Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B.,
RA   Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S.,
RA   Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M.,
RA   Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G.,
RA   Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J.,
RA   Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L.,
RA   Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J.,
RA   Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   FUNCTION.
RX   PubMed=11029034;
RA   Baumann P., Cech T.R.;
RT   "Protection of telomeres by the Ku protein in fission yeast.";
RL   Mol. Biol. Cell 11:3265-3275(2000).
CC   -!- FUNCTION: Involved in ds DNA break repair.
CC   -!- CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n) +
CC       (deoxyribonucleotide)(m) = AMP + diphosphate +
CC       (deoxyribonucleotide)(n+m).
CC   -!- COFACTOR: Magnesium (By similarity).
CC   -!- SUBCELLULAR LOCATION: Nucleus (By similarity).
CC   -!- SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
CC   -!- SIMILARITY: Contains 1 BRCT domain.
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DR   EMBL; CU329672; CAA21085.2; -; Genomic_DNA.
DR   PIR; T40845; T40845.
DR   RefSeq; NP_587888.1; -.
DR   GeneID; 2539042; -.
DR   KEGG; spo:SPCC1183.05c; -.
DR   NMPDR; fig|4896.1.peg.226; -.
DR   GeneDB_Spombe; SPCC1183.05c; -.
DR   OMA; O74833; RRLKDYS.
DR   BRENDA; 6.5.1.1; 653.
DR   ArrayExpress; O74833; -.
DR   GO; GO:0032807; C:DNA ligase IV complex; TAS:GeneDB_SPombe.
DR   GO; GO:0005524; F:ATP binding; IC:GeneDB_SPombe.
DR   GO; GO:0003910; F:DNA ligase (ATP) activity; IEA:EC.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0006303; P:double-strand break repair via nonhomologou...; IMP:GeneDB_SPombe.
DR   InterPro; IPR001357; BRCT.
DR   InterPro; IPR000977; DNA_ligase.
DR   InterPro; IPR012309; DNA_ligase_A_C.
DR   InterPro; IPR012310; DNA_ligase_A_M.
DR   InterPro; IPR016059; DNA_ligase_CS.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   Gene3D; G3DSA:2.40.50.140; OB_NA_bd_sub; 1.
DR   Pfam; PF00533; BRCT; 1.
DR   Pfam; PF04679; DNA_ligase_A_C; 1.
DR   Pfam; PF01068; DNA_ligase_A_M; 1.
DR   TIGRFAMs; TIGR00574; dnl1; 1.
DR   PROSITE; PS50172; BRCT; 1.
DR   PROSITE; PS00697; DNA_LIGASE_A1; 1.
DR   PROSITE; PS00333; DNA_LIGASE_A2; FALSE_NEG.
DR   PROSITE; PS50160; DNA_LIGASE_A3; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Complete proteome; DNA damage; DNA recombination;
KW   DNA repair; DNA replication; Ligase; Magnesium; Metal-binding;
KW   Nucleotide-binding; Nucleus.
FT   CHAIN         1    923       DNA ligase 4.
FT                                /FTId=PRO_0000059581.
FT   DOMAIN      674    767       BRCT.
FT   ACT_SITE    295    295       N6-AMP-lysine intermediate (By
FT                                similarity).
FT   METAL       355    355       Magnesium 1 (Potential).
FT   METAL       452    452       Magnesium 2 (Potential).
FT   BINDING     293    293       ATP (By similarity).
FT   BINDING     300    300       ATP (By similarity).
FT   BINDING     315    315       ATP (By similarity).
FT   BINDING     457    457       ATP (By similarity).
FT   BINDING     468    468       ATP (By similarity).
FT   BINDING     474    474       ATP (By similarity).
SQ   SEQUENCE   923 AA;  107259 MW;  6ED79683AB57DCE0 CRC64;
     MDEAKETVFT KPQNHSSTLE FYDFVTTLLE PLSRIGKTRK SKTSNLDPYE LKRKILLDYF
     NKWRQHVGPD LYPLLRLSNL IFLDRERGSY GFKEFGLGKL FIRAMHLSPT SEDAKSLKNW
     RGSESKHTGD FSTMLQDILQ RRAYRTFPGA FTVGDVNALL DQLADASSEY VLNFLPYLTL
     IRDTRVNILE QFYRSLSPLE LRWLIPILLK VRKYGTSEKF ILSVFHPDAA RLYRLCSSLK
     RICWELYDPS RSLDETETDV EVFSCFQPQL ANFKKKDLHQ TLEAMGNKPF WIEEKLDGER
     IQLHMSSGKF QFYSRNARSY TYAYGSSYFD EQSRLTQYII GAFDKRISQI ILDGEMVTWD
     PVLETVIPYG SLRSIFEDSS SHSSYSPYYV VFDILYLNGK SLVKYSLESR RRILEKVIVR
     ESHRMSILPY KVGSTIEDIE AELRNVIQEG SEGLVIKKPS GSYHLGERMD DWIKVKPYYL
     QGFGEDLDCL ILGGYFGRGK QSGKINSFLC GLRMDYTPKD HSEKFQSFVR VGGGFTYFDR
     DIIRKETEGK WLPWSSDALE YMELAGTKQD FEKPDMWIHP KDSLVLQIKA AEVVVSNRFK
     TNYTLRFPRL EKVRLDRSWK DALTINEFFT LKNAVEKQDN VSFHVNKKRK VSQKREKQKK
     FLYDEPTFKK EASPHSDVLK NLHFVVLPPT ELHETKAGLQ QIIIENGGLI HQGVGNFGKE
     RLFLVADRVS TRVSIERSKN MCTIIRSQWV MDSVNNQRLM PQWSYLLFSK DEKYSWKTAL
     ESLSAKSLSN LLVELKQLDL SKEYSKISDD TSILNLTISK EEASFVGAFP FLKFTVFLDL
     KGIENSELYD VRMGQYRLTK CILLWNGATI EKDISSKKLT HVVMFVEDST RLEQLTKACE
     LTQHRKFTQP SYFQRYGGSF CIP
//