Reviewed, UniProtKB/Swiss-Prot O74833 (DNLI4_SCHPO)

Names and origin

Protein names

Recommended name:
    DNA ligase 4
    EC=6.5.1.1
Alternative name(s):
    DNA ligase IV
    Polydeoxyribonucleotide synthase [ATP] 4

Gene names

Name:	lig4
ORF Names:	SPCC1183.05c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	923 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in ds DNA break repair. Ref.2
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Cofactor	Magnesium By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family. Contains 1 BRCT domain.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair DNA replication
Cellular component	Nucleus
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW double-strand break repair via nonhomologous end joining Ref.2 Inferred from mutant phenotype. Source: GeneDB_SPombe
Cellular component	DNA ligase IV complex Traceable author statement. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred by curator. Source: GeneDB_SPombe DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 923	923	DNA ligase 4		PRO_0000059581
Regions
	Domain	674 – 767	94	BRCT
Sites
	Active site	295	1	N6-AMP-lysine intermediate By similarity
	Metal binding	355	1	Magnesium 1 Potential
	Metal binding	452	1	Magnesium 2 Potential
	Binding site	293	1	ATP By similarity
	Binding site	300	1	ATP By similarity
	Binding site	315	1	ATP By similarity
	Binding site	457	1	ATP By similarity
	Binding site	468	1	ATP By similarity
	Binding site	474	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O74833-1 [UniParc]. Last modified May 1, 2000. Version 2. Checksum: 6ED79683AB57DCE0	FASTA	923	107,259
	10 20 30 40 50 60 MDEAKETVFT KPQNHSSTLE FYDFVTTLLE PLSRIGKTRK SKTSNLDPYE LKRKILLDYF 70 80 90 100 110 120 NKWRQHVGPD LYPLLRLSNL IFLDRERGSY GFKEFGLGKL FIRAMHLSPT SEDAKSLKNW 130 140 150 160 170 180 RGSESKHTGD FSTMLQDILQ RRAYRTFPGA FTVGDVNALL DQLADASSEY VLNFLPYLTL 190 200 210 220 230 240 IRDTRVNILE QFYRSLSPLE LRWLIPILLK VRKYGTSEKF ILSVFHPDAA RLYRLCSSLK 250 260 270 280 290 300 RICWELYDPS RSLDETETDV EVFSCFQPQL ANFKKKDLHQ TLEAMGNKPF WIEEKLDGER 310 320 330 340 350 360 IQLHMSSGKF QFYSRNARSY TYAYGSSYFD EQSRLTQYII GAFDKRISQI ILDGEMVTWD 370 380 390 400 410 420 PVLETVIPYG SLRSIFEDSS SHSSYSPYYV VFDILYLNGK SLVKYSLESR RRILEKVIVR 430 440 450 460 470 480 ESHRMSILPY KVGSTIEDIE AELRNVIQEG SEGLVIKKPS GSYHLGERMD DWIKVKPYYL 490 500 510 520 530 540 QGFGEDLDCL ILGGYFGRGK QSGKINSFLC GLRMDYTPKD HSEKFQSFVR VGGGFTYFDR 550 560 570 580 590 600 DIIRKETEGK WLPWSSDALE YMELAGTKQD FEKPDMWIHP KDSLVLQIKA AEVVVSNRFK 610 620 630 640 650 660 TNYTLRFPRL EKVRLDRSWK DALTINEFFT LKNAVEKQDN VSFHVNKKRK VSQKREKQKK 670 680 690 700 710 720 FLYDEPTFKK EASPHSDVLK NLHFVVLPPT ELHETKAGLQ QIIIENGGLI HQGVGNFGKE 730 740 750 760 770 780 RLFLVADRVS TRVSIERSKN MCTIIRSQWV MDSVNNQRLM PQWSYLLFSK DEKYSWKTAL 790 800 810 820 830 840 ESLSAKSLSN LLVELKQLDL SKEYSKISDD TSILNLTISK EEASFVGAFP FLKFTVFLDL 850 860 870 880 890 900 KGIENSELYD VRMGQYRLTK CILLWNGATI EKDISSKKLT HVVMFVEDST RLEQLTKACE 910 920 LTQHRKFTQP SYFQRYGGSF CIP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[2]	"Protection of telomeres by the Ku protein in fission yeast." Baumann P., Cech T.R. Mol. Biol. Cell 11:3265-3275(2000) [PubMed: 11029034] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329672 Genomic DNA. Translation: CAA21085.2.
PIR	T40845.
RefSeq	NP_587888.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O74833.
Genome annotation databases
GeneID	2539042.
GenomeReviews	Gene locus lig4 in contig CU329672_GR.
KEGG	spo:SPCC1183.05c.
NMPDR	fig|4896.1.peg.226.
Organism-specific databases
GeneDB_Spombe	SPCC1183.05c.
Phylogenomic databases
OMA	RRLKDYS.
Enzyme and pathway databases
BRENDA	6.5.1.1. 653.
Gene expression databases
ArrayExpress	O74833.
Family and domain databases
InterPro	IPR001357. BRCT. IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF00533. BRCT. 1 hit. PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS50172. BRCT. 1 hit. PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. False negative. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI4_SCHPO

Accession

Primary (citable) accession number: O74833

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	May 1, 2000
Last modified:	November 3, 2009
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)