Reviewed, UniProtKB/Swiss-Prot O74827 (CHO1_SCHPO)

Names and origin

Protein names

Recommended name:
    Methylene-fatty-acyl-phospholipid synthase
    EC=2.1.1.16
Alternative name(s):
    Unsaturated phospholipid methyltransferase

Gene names

Name:	cho1
ORF Names:	SPBC337.16

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid methylene fatty acid.
Pathway	Phospholipid metabolism; phosphatidylcholine biosynthesis.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the PEMT/PEM2 methyltransferase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Membrane
Domain	Transmembrane
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphatidylcholine biosynthetic process Ref.1 Inferred from genetic interaction. Source: GeneDB_SPombe
Cellular component	endoplasmic reticulum Inferred from direct assay. Source: GeneDB_SPombe integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	N-methyltransferase activity Inferred from electronic annotation. Source: InterPro methylene-fatty-acyl-phospholipid synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 221	221	Methylene-fatty-acyl-phospholipid synthase		PRO_0000193923
Regions
	Transmembrane	33 – 53	21	Potential
	Transmembrane	73 – 93	21	Potential
	Transmembrane	116 – 136	21	Potential
	Transmembrane	178 – 198	21	Potential
Experimental info
	Sequence conflict	160	1	D → DND Ref.1
	Sequence conflict	202 – 221	20	Missing Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O74827-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 26CEEDF270FE9AD7	FASTA	221	24,767
	10 20 30 40 50 60 MSLILYPKPT SYLYQPFIKA YFSLNMAIFE INNSFLICAV SIALNPLLWN IAARSEYNHK 70 80 90 100 110 120 TLTKLANGDS KKACYMLAAC IFVAGIVRDL IYQNALKQQP TLGIFMNPLV QGIAKLIFCF 130 140 150 160 170 180 GSVLVLSSMY KLGLVGTYLG DYFGFLLPER VSGFPFNVND NPMYNGSTLC FLSTALRYGK 190 200 210 220 VAGLLLTLEV FFVYRIALKF EEPFTAKIYA ARDSKQAKKS E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Schizosaccharomyces pombe cho1+ gene encodes a phospholipid methyltransferase." Kanipes M.I., Hill J.E., Henry S.A. Genetics 150:553-562(1998) [PubMed: 9755189] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF004112 Genomic DNA. Translation: AAB61409.1. CU329671 Genomic DNA. Translation: CAA21286.1.
PIR	T40269.
RefSeq	NP_595417.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2540995.
KEGG	spo:SPBC337.16.
NMPDR	fig|4896.1.peg.1283.
Organism-specific databases
GeneDB_Spombe	SPBC337.16.
Phylogenomic databases
OMA	O74827. VARWEHK.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-003489-MON.
BRENDA	2.1.1.16. 653.
Gene expression databases
ArrayExpress	O74827.
Family and domain databases
InterPro	IPR007318. PEMT. [Graphical view]
PANTHER	PTHR15458. PEMT. 1 hit.
Pfam	PF04191. PEMT. 1 hit. [Graphical view]
PIRSF	PIRSF005444. PEMT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CHO1_SCHPO

Accession

Primary (citable) accession number: O74827
Secondary accession number(s): P87300

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	November 1, 1998
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)