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Protein

Phosphatidyl-N-methylethanolamine N-methyltransferase

Gene

cho1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the second two steps of the methylation pathway of phosphatidylcholine biosynthesis, the SAM-dependent methylation of phosphatidylmonomethylethanolamine (PMME) to phosphatidyldimethylethanolamine (PDME) and of PDME to phosphatidylcholine (PC).UniRule annotation1 Publication

Catalytic activityi

S-adenosyl-L-methionine + phosphatidyl-N-methylethanolamine = S-adenosyl-L-homocysteine + phosphatidyl-N-dimethylethanolamine.UniRule annotation1 Publication
S-adenosyl-L-methionine + phosphatidyl-N-dimethylethanolamine = S-adenosyl-L-homocysteine + phosphatidylcholine.UniRule annotation1 Publication

Pathwayi: phosphatidylcholine biosynthesis

This protein is involved in the pathway phosphatidylcholine biosynthesis, which is part of Phospholipid metabolism.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

  • phosphatidylcholine biosynthetic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-SPO-1483191. Synthesis of PC.
UniPathwayiUPA00753.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidyl-N-methylethanolamine N-methyltransferaseUniRule annotationCurated (EC:2.1.1.71UniRule annotation1 Publication)
Alternative name(s):
Phospholipid methyltransferase1 PublicationUniRule annotation
Short name:
PLMTUniRule annotation
Gene namesi
Name:cho11 Publication
ORF Names:SPBC337.16Imported
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC337.16.
PomBaseiSPBC337.16. cho1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 26LumenalUniRule annotationAdd BLAST26
Intramembranei27 – 53HelicalUniRule annotationAdd BLAST27
Topological domaini54 – 65LumenalUniRule annotationAdd BLAST12
Transmembranei66 – 87HelicalUniRule annotationAdd BLAST22
Topological domaini88 – 114CytoplasmicUniRule annotationAdd BLAST27
Transmembranei115 – 135HelicalUniRule annotationAdd BLAST21
Topological domaini136 – 178LumenalUniRule annotationAdd BLAST43
Transmembranei179 – 199HelicalUniRule annotationAdd BLAST21
Topological domaini200 – 221CytoplasmicUniRule annotationAdd BLAST22

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001939231 – 221Phosphatidyl-N-methylethanolamine N-methyltransferaseAdd BLAST221

Proteomic databases

MaxQBiO74827.
PRIDEiO74827.

Interactioni

Protein-protein interaction databases

BioGridi277511. 95 interactors.
MINTiMINT-4681043.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni119 – 121S-adenosyl-L-methionine bindingUniRule annotation3
Regioni201 – 202S-adenosyl-L-methionine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the class VI-like SAM-binding methyltransferase superfamily. PEMT/PEM2 methyltransferase family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000208789.
InParanoidiO74827.
KOiK00550.
OMAiTSTWALG.
OrthoDBiEOG092C45C1.
PhylomeDBiO74827.

Family and domain databases

HAMAPiMF_03216. PLMT. 1 hit.
InterProiIPR024960. PEMT/MFAP.
IPR007318. Phopholipid_MeTrfase.
[Graphical view]
PANTHERiPTHR15458. PTHR15458. 1 hit.
PfamiPF04191. PEMT. 1 hit.
[Graphical view]
PIRSFiPIRSF005444. PEMT. 1 hit.
PROSITEiPS51599. SAM_PEMT_PEM2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O74827-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLILYPKPT SYLYQPFIKA YFSLNMAIFE INNSFLICAV SIALNPLLWN
60 70 80 90 100
IAARSEYNHK TLTKLANGDS KKACYMLAAC IFVAGIVRDL IYQNALKQQP
110 120 130 140 150
TLGIFMNPLV QGIAKLIFCF GSVLVLSSMY KLGLVGTYLG DYFGFLLPER
160 170 180 190 200
VSGFPFNVND NPMYNGSTLC FLSTALRYGK VAGLLLTLEV FFVYRIALKF
210 220
EEPFTAKIYA ARDSKQAKKS E
Length:221
Mass (Da):24,767
Last modified:November 1, 1998 - v1
Checksum:i26CEEDF270FE9AD7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti160D → DND in AAB61409 (PubMed:9755189).Curated1
Sequence conflicti202 – 221Missing in AAB61409 (PubMed:9755189).CuratedAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF004112 Genomic DNA. Translation: AAB61409.1.
CU329671 Genomic DNA. Translation: CAA21286.1.
PIRiT40269.
RefSeqiNP_595417.1. NM_001021324.2.

Genome annotation databases

EnsemblFungiiSPBC337.16.1; SPBC337.16.1:pep; SPBC337.16.
GeneIDi2540995.
KEGGispo:SPBC337.16.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF004112 Genomic DNA. Translation: AAB61409.1.
CU329671 Genomic DNA. Translation: CAA21286.1.
PIRiT40269.
RefSeqiNP_595417.1. NM_001021324.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277511. 95 interactors.
MINTiMINT-4681043.

Proteomic databases

MaxQBiO74827.
PRIDEiO74827.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC337.16.1; SPBC337.16.1:pep; SPBC337.16.
GeneIDi2540995.
KEGGispo:SPBC337.16.

Organism-specific databases

EuPathDBiFungiDB:SPBC337.16.
PomBaseiSPBC337.16. cho1.

Phylogenomic databases

HOGENOMiHOG000208789.
InParanoidiO74827.
KOiK00550.
OMAiTSTWALG.
OrthoDBiEOG092C45C1.
PhylomeDBiO74827.

Enzyme and pathway databases

UniPathwayiUPA00753.
ReactomeiR-SPO-1483191. Synthesis of PC.

Miscellaneous databases

PROiO74827.

Family and domain databases

HAMAPiMF_03216. PLMT. 1 hit.
InterProiIPR024960. PEMT/MFAP.
IPR007318. Phopholipid_MeTrfase.
[Graphical view]
PANTHERiPTHR15458. PTHR15458. 1 hit.
PfamiPF04191. PEMT. 1 hit.
[Graphical view]
PIRSFiPIRSF005444. PEMT. 1 hit.
PROSITEiPS51599. SAM_PEMT_PEM2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLMT_SCHPO
AccessioniPrimary (citable) accession number: O74827
Secondary accession number(s): P87300
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.