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Protein

Phosphatidyl-N-methylethanolamine N-methyltransferase

Gene

cho1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the second two steps of the methylation pathway of phosphatidylcholine biosynthesis, the SAM-dependent methylation of phosphatidylmonomethylethanolamine (PMME) to phosphatidyldimethylethanolamine (PDME) and of PDME to phosphatidylcholine (PC).UniRule annotation1 Publication

Catalytic activityi

S-adenosyl-L-methionine + phosphatidyl-N-methylethanolamine = S-adenosyl-L-homocysteine + phosphatidyl-N-dimethylethanolamine.UniRule annotation1 Publication
S-adenosyl-L-methionine + phosphatidyl-N-dimethylethanolamine = S-adenosyl-L-homocysteine + phosphatidylcholine.UniRule annotation1 Publication

Pathwayi: phosphatidylcholine biosynthesis

This protein is involved in the pathway phosphatidylcholine biosynthesis, which is part of Phospholipid metabolism.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

  • phosphatidylcholine biosynthetic process Source: PomBase

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-SPO-1483191 Synthesis of PC
UniPathwayiUPA00753

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidyl-N-methylethanolamine N-methyltransferaseUniRule annotationCurated (EC:2.1.1.71UniRule annotation1 Publication)
Alternative name(s):
Phospholipid methyltransferase1 PublicationUniRule annotation
Short name:
PLMTUniRule annotation
Gene namesi
Name:cho11 Publication
ORF Names:SPBC337.16Imported
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC337.16
PomBaseiSPBC337.16 cho1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 26LumenalUniRule annotationAdd BLAST26
Intramembranei27 – 53HelicalUniRule annotationAdd BLAST27
Topological domaini54 – 65LumenalUniRule annotationAdd BLAST12
Transmembranei66 – 87HelicalUniRule annotationAdd BLAST22
Topological domaini88 – 114CytoplasmicUniRule annotationAdd BLAST27
Transmembranei115 – 135HelicalUniRule annotationAdd BLAST21
Topological domaini136 – 178LumenalUniRule annotationAdd BLAST43
Transmembranei179 – 199HelicalUniRule annotationAdd BLAST21
Topological domaini200 – 221CytoplasmicUniRule annotationAdd BLAST22

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001939231 – 221Phosphatidyl-N-methylethanolamine N-methyltransferaseAdd BLAST221

Proteomic databases

MaxQBiO74827
PaxDbiO74827
PRIDEiO74827

Interactioni

Protein-protein interaction databases

BioGridi277511, 95 interactors
STRINGi4896.SPBC337.16.1

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni119 – 121S-adenosyl-L-methionine bindingUniRule annotation3
Regioni201 – 202S-adenosyl-L-methionine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the class VI-like SAM-binding methyltransferase superfamily. PEMT/PEM2 methyltransferase family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000208789
InParanoidiO74827
KOiK00551
OMAiPFTEEIY
OrthoDBiEOG092C45C1
PhylomeDBiO74827

Family and domain databases

HAMAPiMF_03216 PLMT, 1 hit
InterProiView protein in InterPro
IPR024960 PEMT/MFAP
IPR007318 Phopholipid_MeTrfase
PANTHERiPTHR15458 PTHR15458, 1 hit
PfamiView protein in Pfam
PF04191 PEMT, 1 hit
PIRSFiPIRSF005444 PEMT, 1 hit
PROSITEiView protein in PROSITE
PS51599 SAM_PEMT_PEM2, 1 hit

Sequencei

Sequence statusi: Complete.

O74827-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLILYPKPT SYLYQPFIKA YFSLNMAIFE INNSFLICAV SIALNPLLWN
60 70 80 90 100
IAARSEYNHK TLTKLANGDS KKACYMLAAC IFVAGIVRDL IYQNALKQQP
110 120 130 140 150
TLGIFMNPLV QGIAKLIFCF GSVLVLSSMY KLGLVGTYLG DYFGFLLPER
160 170 180 190 200
VSGFPFNVND NPMYNGSTLC FLSTALRYGK VAGLLLTLEV FFVYRIALKF
210 220
EEPFTAKIYA ARDSKQAKKS E
Length:221
Mass (Da):24,767
Last modified:November 1, 1998 - v1
Checksum:i26CEEDF270FE9AD7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti160D → DND in AAB61409 (PubMed:9755189).Curated1
Sequence conflicti202 – 221Missing in AAB61409 (PubMed:9755189).CuratedAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF004112 Genomic DNA Translation: AAB61409.1
CU329671 Genomic DNA Translation: CAA21286.1
PIRiT40269
RefSeqiNP_595417.1, NM_001021324.2

Genome annotation databases

EnsemblFungiiSPBC337.16.1; SPBC337.16.1:pep; SPBC337.16
GeneIDi2540995
KEGGispo:SPBC337.16

Similar proteinsi

Entry informationi

Entry nameiPLMT_SCHPO
AccessioniPrimary (citable) accession number: O74827
Secondary accession number(s): P87300
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1998
Last modified: March 28, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health