O74794 (CCHL_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative cytochrome c heme lyase
Short name=CCHL
EC=4.4.1.17

Alternative name(s):
Holocytochrome-c synthase

Gene names

ORF Names:

SPBC26H8.12

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Links covalently the heme group to the apoprotein of cytochrome c By similarity.
Catalytic activity	Holocytochrome c = apocytochrome c + heme.
Subcellular location	Mitochondrion inner membrane By similarity.
Sequence similarities	Belongs to the cytochrome c-type heme lyase family. Contains 2 HRM (heme regulatory motif) repeats.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Repeat
Ligand	Heme Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cytochrome c-heme linkage Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial intermembrane space Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Molecular function	holocytochrome-c synthase activity Inferred from sequence or structural similarity. Source: GeneDB_Spombe metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	Putative cytochrome c heme lyase		PRO_0000121716
Regions
	Repeat	114 – 119	6	HRM 1
	Repeat	124 – 129	6	HRM 2

Sequences

	Sequence		Length	Mass (Da)
	O74794 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: F8F44897D3BC1682	FASTA	377	42,880
	10 20 30 40 50 60 MTSSETTTDH PRTGKCPIDH SKFARSNEAN PDAYINGIKK DQQSSSWWNS LWSRNTDVAS 70 80 90 100 110 120 EPDVAMLHKK PSTVDTHDHP LANPPPGCPM HKASNENSTG FFSNLFGREK QNSEATPAVQ 130 140 150 160 170 180 PPATCPMSNS NQKPAGVSEV LTGVDSKQQS YVPEGCPVAT PKRGWFNWFG NNDDQKQEAY 190 200 210 220 230 240 EVDKSNMMYK NIPQTAVDDQ VVGLETTRTT SSIPKVDGKN WEYPSPQQMY NAMWRKGYRD 250 260 270 280 290 300 SGENVPIMVQ VHNFLNEGAW SEIKAWEREA GENTEPKLLR FEGNANKRTP RALWYMMLGR 310 320 330 340 350 360 INPNRWGSGE GPFDRHDWYV QRKDNSIVRY VIDYYEAPDS ADGKPVFSLD VRPAVDSFES 370 VALRWKHWRA MRQMQQQ

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAA21104.1.
PIR	T40024.
RefSeq	NP_596655.1. NM_001022577.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O74794.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC26H8.12.1; SPBC26H8.12.1:pep; SPBC26H8.12.
GeneID	2540445.
KEGG	spo:SPBC26H8.12.
NMPDR	fig|4896.1.peg.2521.
Organism-specific databases
GeneDB_Spombe	SPBC26H8.12.
Phylogenomic databases
eggNOG	fuNOG07296.
GeneTree	EFGT00050000005300.
HOGENOM	HBG627179.
OMA	AGENTEP.
OrthoDB	EOG4MSH77.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-004643-MONOMER.
Gene expression databases
ArrayExpress	O74794.
Family and domain databases
InterPro	IPR000511. Cyt_C/C1_haem_lyase. [Graphical view]
KO	K01764.
PANTHER	PTHR12743. Cyto_heme_lyase. 1 hit.
Pfam	PF01265. Cyto_heme_lyase. 1 hit. [Graphical view]
PROSITE	PS00821. CYTO_HEME_LYASE_1. 1 hit. PS00822. CYTO_HEME_LYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CCHL_SCHPO

Accession

Primary (citable) accession number: O74794

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	November 1, 1998
Last modified:	December 14, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program