Reviewed, UniProtKB/Swiss-Prot O74787 (CHO2_SCHPO)

Names and origin

Protein names

Recommended name:
    Phosphatidylethanolamine N-methyltransferase
      Short name=PEAMT
    EC=2.1.1.17

Gene names

Name:	cho2
ORF Names:	SPBC26H8.03

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	905 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + phosphatidylethanolamine = S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine.
Pathway	Phospholipid metabolism; phosphatidylcholine biosynthesis.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphatidylcholine biosynthetic process Traceable author statement. Source: GeneDB_SPombe
Cellular component	endoplasmic reticulum membrane Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	phosphatidylethanolamine N-methyltransferase activity Inferred from genetic interaction. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 905	905	Phosphatidylethanolamine N-methyltransferase		PRO_0000089647
Amino acid modifications
	Modified residue	353	1	Phosphoserine Ref.3
Experimental info
	Sequence conflict	133	1	C → G in AAB61410. Ref.2
	Sequence conflict	137	1	W → G in AAB61410. Ref.2
	Sequence conflict	149	1	L → Q in AAB61410. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O74787-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: E46CF4AEC0CFE889	FASTA	905	102,762
	10 20 30 40 50 60 MTNQIPSASS AADFGSSKST SVDAVPNMDK SSSVRRKNID SNGLQQTNQI EQAESSLNAE 70 80 90 100 110 120 ADHSEPERYG CTPSGKVFLL PKEQENRRSI LETVDPRFSK TPWDWIVISS ILAQVLLFFM 130 140 150 160 170 180 TTGAVRRYSM MLCFFFWRIS YDAGIGFLLH MQSNHRKVVT WISDFGFFDK ENHPKLYDLT 190 200 210 220 230 240 KKQLISKMDS SYNYDTSPLE FNSWLVFRHF VDLILMCDFC SYILMGLAWT CWPKVNIILQ 250 260 270 280 290 300 FLRIFGGIAL IVFNYWVKMD AHRVVRDYAW YWGDFFFLLR SSLVFNGVFE LAPHPMYSVG 310 320 330 340 350 360 YAGYYGMSLL TGSYAVLFAS ILAHAAQFGF LLFVENPHIE RTYGTDINHA RLSPRGEDNE 370 380 390 400 410 420 FELPPEHDLV GFVNFDFTRI SDVALLIIAL YSIFIILLSS NSHYSQFWAI FQAFVWRFLH 430 440 450 460 470 480 SIIHAFILFY QSKSKAWTKH FIRNGESAAY AWSQWKGLYN LTLNMSYISF VMAAWKLYHL 490 500 510 520 530 540 PSNWTYGLVS LRHALGFGLI ALHIYTSVSI YEDLGQYGWF YGDFFLPSRS PKLVYQGIYR 550 560 570 580 590 600 YVNNPERFLG CSAYWGLALI SSSAWIFLIA ILAQLSNLAI IRLVEQPHMQ KVYGNTLRKE 610 620 630 640 650 660 AGISKLIKQA TSEKGNILPK TVETHMKALT TSVDKVLDQT AEALEEFVNT APPKVQELLK 670 680 690 700 710 720 GTESNLRKNA QLAILKLFAP QLSSSTHFDY KLEIKGIDNN QVLLGHPITV CWTASPNHEI 730 740 750 760 770 780 NDWIGLYKLS DNASDLYTQT SSEGRWSAID ANGYTSHCSS IKSLSNDKNS GEVEFSGDLL 790 800 810 820 830 840 FWETGTFEFR YHYGGKHLVM AKTEPFVITA TSMNTTDVDE VSAYLLKSIK FCDPNITPHD 850 860 870 880 890 900 GDASLCDISE GSARKLTSII KYSFGIDLSY RVVQVDGSCS ALSRRIVNSL KILQSFDGPS GAKDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[2]	"The Schizosaccharomyces pombe cho2+ gene encodes a phosphatidylethanolamine methyltransferase." Kanipes M.I., Henry S.A. Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 130-905.
[3]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-353, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329671 Genomic DNA. Translation: CAA21095.1. AF004113 Genomic DNA. Translation: AAB61410.1.
PIR	T40015.
RefSeq	NP_596644.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2540538.
KEGG	spo:SPBC26H8.03.
NMPDR	fig|4896.1.peg.2510.
Organism-specific databases
GeneDB_Spombe	SPBC26H8.03.
Phylogenomic databases
OMA	O74787. APHPMYS.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-004632-MON.
BRENDA	2.1.1.17. 653.
Gene expression databases
ArrayExpress	O74787.
Family and domain databases
InterPro	IPR016219. Phosphatid-EA_MeTrfase_fun. [Graphical view]
PIRSF	PIRSF000383. PEAMT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CHO2_SCHPO

Accession

Primary (citable) accession number: O74787
Secondary accession number(s): P87301

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	November 1, 1998
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)