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O74787 (CHO2_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphatidylethanolamine N-methyltransferase
Short name=PEAMT
EC=2.1.1.17
Gene names
Name:cho2
ORF Names:SPBC26H8.03
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length905 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis By similarity.

Catalytic activity

S-adenosyl-L-methionine + phosphatidylethanolamine = S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine.

Pathway

Phospholipid metabolism; phosphatidylcholine biosynthesis.

Subcellular location

Membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the CHO2 family.

Ontologies

Keywords
   Biological processPhospholipid biosynthesis
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processphosphatidylcholine biosynthetic process

Traceable author statement. Source: GeneDB_Spombe

   Cellular componentendoplasmic reticulum membrane

Inferred from direct assay. Source: GeneDB_Spombe

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionphosphatidylethanolamine N-methyltransferase activity

Inferred from genetic interaction. Source: GeneDB_Spombe

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 905905Phosphatidylethanolamine N-methyltransferase
PRO_0000089647

Regions

Topological domain1 – 104104Extracellular Potential
Transmembrane105 – 12521Helical; Potential
Topological domain126 – 1283Cytoplasmic Potential
Transmembrane129 – 14921Helical; Potential
Topological domain150 – 20960Extracellular Potential
Transmembrane210 – 23021Helical; Potential
Topological domain231 – 2366Cytoplasmic Potential
Transmembrane237 – 25721Helical; Potential
Topological domain258 – 26811Extracellular Potential
Transmembrane269 – 28921Helical; Potential
Topological domain290 – 31324Cytoplasmic Potential
Transmembrane314 – 33421Helical; Potential
Topological domain335 – 37945Extracellular Potential
Transmembrane380 – 40021Helical; Potential
Topological domain401 – 4088Cytoplasmic Potential
Transmembrane409 – 42921Helical; Potential
Topological domain430 – 45627Extracellular Potential
Transmembrane457 – 47923Helical; Potential
Topological domain480 – 49314Cytoplasmic Potential
Transmembrane494 – 51421Helical; Potential
Topological domain515 – 55238Extracellular Potential
Transmembrane553 – 57321Helical; Potential
Topological domain574 – 905332Cytoplasmic Potential

Amino acid modifications

Modified residue3531Phosphoserine Ref.3

Experimental info

Sequence conflict1331C → G in AAB61410. Ref.2
Sequence conflict1371W → G in AAB61410. Ref.2
Sequence conflict1491L → Q in AAB61410. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O74787 [UniParc].

Last modified November 1, 1998. Version 1.
Checksum: E46CF4AEC0CFE889

FASTA905102,762
        10         20         30         40         50         60 
MTNQIPSASS AADFGSSKST SVDAVPNMDK SSSVRRKNID SNGLQQTNQI EQAESSLNAE 

        70         80         90        100        110        120 
ADHSEPERYG CTPSGKVFLL PKEQENRRSI LETVDPRFSK TPWDWIVISS ILAQVLLFFM 

       130        140        150        160        170        180 
TTGAVRRYSM MLCFFFWRIS YDAGIGFLLH MQSNHRKVVT WISDFGFFDK ENHPKLYDLT 

       190        200        210        220        230        240 
KKQLISKMDS SYNYDTSPLE FNSWLVFRHF VDLILMCDFC SYILMGLAWT CWPKVNIILQ 

       250        260        270        280        290        300 
FLRIFGGIAL IVFNYWVKMD AHRVVRDYAW YWGDFFFLLR SSLVFNGVFE LAPHPMYSVG 

       310        320        330        340        350        360 
YAGYYGMSLL TGSYAVLFAS ILAHAAQFGF LLFVENPHIE RTYGTDINHA RLSPRGEDNE 

       370        380        390        400        410        420 
FELPPEHDLV GFVNFDFTRI SDVALLIIAL YSIFIILLSS NSHYSQFWAI FQAFVWRFLH 

       430        440        450        460        470        480 
SIIHAFILFY QSKSKAWTKH FIRNGESAAY AWSQWKGLYN LTLNMSYISF VMAAWKLYHL 

       490        500        510        520        530        540 
PSNWTYGLVS LRHALGFGLI ALHIYTSVSI YEDLGQYGWF YGDFFLPSRS PKLVYQGIYR 

       550        560        570        580        590        600 
YVNNPERFLG CSAYWGLALI SSSAWIFLIA ILAQLSNLAI IRLVEQPHMQ KVYGNTLRKE 

       610        620        630        640        650        660 
AGISKLIKQA TSEKGNILPK TVETHMKALT TSVDKVLDQT AEALEEFVNT APPKVQELLK 

       670        680        690        700        710        720 
GTESNLRKNA QLAILKLFAP QLSSSTHFDY KLEIKGIDNN QVLLGHPITV CWTASPNHEI 

       730        740        750        760        770        780 
NDWIGLYKLS DNASDLYTQT SSEGRWSAID ANGYTSHCSS IKSLSNDKNS GEVEFSGDLL 

       790        800        810        820        830        840 
FWETGTFEFR YHYGGKHLVM AKTEPFVITA TSMNTTDVDE VSAYLLKSIK FCDPNITPHD 

       850        860        870        880        890        900 
GDASLCDISE GSARKLTSII KYSFGIDLSY RVVQVDGSCS ALSRRIVNSL KILQSFDGPS 


GAKDD

« Hide

References

« Hide 'large scale' references
[1]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed: 11859360] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"The Schizosaccharomyces pombe cho2+ gene encodes a phosphatidylethanolamine methyltransferase."
Kanipes M.I., Henry S.A.
Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 130-905.
[3]"Phosphoproteome analysis of fission yeast."
Wilson-Grady J.T., Villen J., Gygi S.P.
J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-353, MASS SPECTROMETRY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CU329671 Genomic DNA. Translation: CAA21095.1.
AF004113 Genomic DNA. Translation: AAB61410.1.
PIRT40015.
RefSeqNP_596644.1. NM_001022566.1.

3D structure databases

ProteinModelPortalO74787.
ModBaseSearch...

Protein-protein interaction databases

STRINGO74787.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPBC26H8.03.1; SPBC26H8.03.1:pep; SPBC26H8.03.
GeneID2540538.
GenomeReviewsGene locus cho2 in contig CU329671_GR.
KEGGspo:SPBC26H8.03.
NMPDRfig|4896.1.peg.2510.

Organism-specific databases

GeneDB_SpombeSPBC26H8.03.

Phylogenomic databases

eggNOGfuNOG06636.
GeneTreeEFGT00050000006273.
HOGENOMHBG738799.
OMAAPHPMYS.
OrthoDBEOG4T4H3J.

Enzyme and pathway databases

BioCycSPOM-XXX-01:SPOM-XXX-01-004632-MONOMER.

Gene expression databases

ArrayExpressO74787.

Family and domain databases

InterProIPR016219. Phosphatid-EA_MeTrfase_fun.
[Graphical view]
KOK00551.
PIRSFPIRSF000383. PEAMT. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCHO2_SCHPO
AccessionPrimary (citable) accession number: O74787
Secondary accession number(s): P87301
Entry history
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: November 1, 1998
Last modified: December 14, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents