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Protein

Probable arginine--tRNA ligase, cytoplasmic

Gene

mrs1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis.By similarity

Catalytic activityi

ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei160L-arginineBy similarity1
Binding sitei359L-arginineBy similarity1
Binding sitei363L-arginineBy similarity1
Binding sitei387L-arginineBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable arginine--tRNA ligase, cytoplasmic (EC:6.1.1.19)
Alternative name(s):
Arginyl-tRNA synthetase
Short name:
ArgRS
Gene namesi
Name:mrs1
Synonyms:rrs1
ORF Names:SPBC25B2.09c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC25B2.09c.
PomBaseiSPBC25B2.09c. mrs1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001516631 – 618Probable arginine--tRNA ligase, cytoplasmicAdd BLAST618

Proteomic databases

MaxQBiO74781.
PRIDEiO74781.

Interactioni

Protein-protein interaction databases

MINTiMINT-4680727.
STRINGi4896.SPBC25B2.09c.1.

Structurei

3D structure databases

ProteinModelPortaliO74781.
SMRiO74781.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni60 – 61Interaction with tRNABy similarity2
Regioni104 – 109Interaction with tRNABy similarity6
Regioni146 – 151L-arginine bindingBy similarity6
Regioni496 – 510Interaction with tRNABy similarityAdd BLAST15

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi149 – 160"HIGH" regionAdd BLAST12

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000247211.
InParanoidiO74781.
KOiK01887.
OMAiAGNQIEN.
OrthoDBiEOG092C0Z3M.
PhylomeDBiO74781.

Family and domain databases

CDDicd00671. ArgRS_core. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.30.1360.70. 1 hit.
3.40.50.620. 1 hit.
InterProiView protein in InterPro
IPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-ligase.
IPR005148. Arg-tRNA-synth_N.
IPR035684. ArgRS_core.
IPR008909. DALR_anticod-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
PANTHERiPTHR11956. PTHR11956. 1 hit.
PfamiView protein in Pfam
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
PRINTSiPR01038. TRNASYNTHARG.
SMARTiView protein in SMART
SM01016. Arg_tRNA_synt_N. 1 hit.
SM00836. DALR_1. 1 hit.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF55190. SSF55190. 1 hit.
TIGRFAMsiTIGR00456. argS. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

O74781-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATSVDQISK SLSTLGLQDL PVFREADIHH NPVDVYRSYI SSELSKINGV
60 70 80 90 100
DVSLIYPALE TSISKDSADL NLPVPRLRVK GKPQELAQKW AEAFPKDLVE
110 120 130 140 150
VTANGIFLRF NFNGPSLTKL ILPIIWEQRE NYGRNESGSG KVAVIEFSSP
160 170 180 190 200
NIAKPFHAGH LRSTIIGSFL ANLHESQGWK VHRVNYLGDW GKQFGLLAIG
210 220 230 240 250
YKKYGDEDQL KSNPIRHLYD VYVKVNADAT EEDEKIQKDK AEAESKGLPY
260 270 280 290 300
TPPLSLHDKA REFFKRMEDG DEESLKVWAR FRDLSITKLK DTYDRLNIHY
310 320 330 340 350
DEYDGESQVS LELMNKMVDE LRSLNLIEED GGALLIDLSK HDKKLGKAIV
360 370 380 390 400
QKRDGTTLYL TRDIGTAYKR YEKYKFDKSI YVVSSQQDMY FSQLFKIFEL
410 420 430 440 450
MGFDWAKKCV HINYGLVQGM STRKGKAVFL DDIMEVAKEE MHKVMQKNEE
460 470 480 490 500
KYAQVENPEE VADIVGKTAI RIQDSTGKRI NNYAFDWSRM TSFEGDTGPY
510 520 530 540 550
LQYAHSRLSS VRRNVNYTDE EIMGANLELL TEPDAYDLVR LLGQYPDVLK
560 570 580 590 600
NAFRFQETST VVTYLFKLTH AVSKLYDILW VRGRERDIQL ARLALFGAAK
610
QVLNNGMTLL GLTPLERM
Length:618
Mass (Da):70,689
Last modified:November 1, 1998 - v1
Checksum:i69BDECEA3B2853F5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti359Y → S in BAA13762 (PubMed:9501991).Curated1
Sequence conflicti401M → T in BAA13762 (PubMed:9501991).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA21267.1.
D89099 mRNA. Translation: BAA13762.1.
PIRiT39985.
T42007.
RefSeqiNP_596077.1. NM_001021989.2.

Genome annotation databases

EnsemblFungiiSPBC25B2.09c.1; SPBC25B2.09c.1:pep; SPBC25B2.09c.
GeneIDi2540548.
KEGGispo:SPBC25B2.09c.

Similar proteinsi

Entry informationi

Entry nameiSYRC_SCHPO
AccessioniPrimary (citable) accession number: O74781
Secondary accession number(s): P78751
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: November 1, 1998
Last modified: August 30, 2017
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families