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O74781

- SYRC_SCHPO

UniProt

O74781 - SYRC_SCHPO

Protein

Probable arginine--tRNA ligase, cytoplasmic

Gene

mrs1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis.By similarity

    Catalytic activityi

    ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).

    GO - Molecular functioni

    1. arginine-tRNA ligase activity Source: PomBase
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. arginyl-tRNA aminoacylation Source: PomBase

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable arginine--tRNA ligase, cytoplasmic (EC:6.1.1.19)
    Alternative name(s):
    Arginyl-tRNA synthetase
    Short name:
    ArgRS
    Gene namesi
    Name:mrs1
    Synonyms:rrs1
    ORF Names:SPBC25B2.09c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC25B2.09c.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: PomBase
    2. cytosol Source: PomBase
    3. mitochondrion Source: PomBase

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 618618Probable arginine--tRNA ligase, cytoplasmicPRO_0000151663Add
    BLAST

    Proteomic databases

    MaxQBiO74781.
    PaxDbiO74781.

    Interactioni

    Protein-protein interaction databases

    BioGridi277075. 3 interactions.
    MINTiMINT-4680727.
    STRINGi4896.SPBC25B2.09c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliO74781.
    SMRiO74781. Positions 10-618.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi149 – 16012"HIGH" regionAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0018.
    HOGENOMiHOG000247211.
    KOiK01887.
    OMAiCEDRGAL.
    OrthoDBiEOG7NKKV8.
    PhylomeDBiO74781.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.30.1360.70. 1 hit.
    3.40.50.620. 1 hit.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR001278. Arg-tRNA-ligase.
    IPR005148. Arg-tRNA-synth_N.
    IPR008909. DALR_anticod-bd.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    [Graphical view]
    PANTHERiPTHR11956. PTHR11956. 1 hit.
    PfamiPF05746. DALR_1. 1 hit.
    PF00750. tRNA-synt_1d. 1 hit.
    [Graphical view]
    PRINTSiPR01038. TRNASYNTHARG.
    SMARTiSM01016. Arg_tRNA_synt_N. 1 hit.
    SM00836. DALR_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF55190. SSF55190. 1 hit.
    TIGRFAMsiTIGR00456. argS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O74781-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATSVDQISK SLSTLGLQDL PVFREADIHH NPVDVYRSYI SSELSKINGV    50
    DVSLIYPALE TSISKDSADL NLPVPRLRVK GKPQELAQKW AEAFPKDLVE 100
    VTANGIFLRF NFNGPSLTKL ILPIIWEQRE NYGRNESGSG KVAVIEFSSP 150
    NIAKPFHAGH LRSTIIGSFL ANLHESQGWK VHRVNYLGDW GKQFGLLAIG 200
    YKKYGDEDQL KSNPIRHLYD VYVKVNADAT EEDEKIQKDK AEAESKGLPY 250
    TPPLSLHDKA REFFKRMEDG DEESLKVWAR FRDLSITKLK DTYDRLNIHY 300
    DEYDGESQVS LELMNKMVDE LRSLNLIEED GGALLIDLSK HDKKLGKAIV 350
    QKRDGTTLYL TRDIGTAYKR YEKYKFDKSI YVVSSQQDMY FSQLFKIFEL 400
    MGFDWAKKCV HINYGLVQGM STRKGKAVFL DDIMEVAKEE MHKVMQKNEE 450
    KYAQVENPEE VADIVGKTAI RIQDSTGKRI NNYAFDWSRM TSFEGDTGPY 500
    LQYAHSRLSS VRRNVNYTDE EIMGANLELL TEPDAYDLVR LLGQYPDVLK 550
    NAFRFQETST VVTYLFKLTH AVSKLYDILW VRGRERDIQL ARLALFGAAK 600
    QVLNNGMTLL GLTPLERM 618
    Length:618
    Mass (Da):70,689
    Last modified:November 1, 1998 - v1
    Checksum:i69BDECEA3B2853F5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti359 – 3591Y → S in BAA13762. (PubMed:9501991)Curated
    Sequence conflicti401 – 4011M → T in BAA13762. (PubMed:9501991)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAA21267.1.
    D89099 mRNA. Translation: BAA13762.1.
    PIRiT39985.
    T42007.
    RefSeqiNP_596077.1. NM_001021989.2.

    Genome annotation databases

    EnsemblFungiiSPBC25B2.09c.1; SPBC25B2.09c.1:pep; SPBC25B2.09c.
    GeneIDi2540548.
    KEGGispo:SPBC25B2.09c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAA21267.1 .
    D89099 mRNA. Translation: BAA13762.1 .
    PIRi T39985.
    T42007.
    RefSeqi NP_596077.1. NM_001021989.2.

    3D structure databases

    ProteinModelPortali O74781.
    SMRi O74781. Positions 10-618.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 277075. 3 interactions.
    MINTi MINT-4680727.
    STRINGi 4896.SPBC25B2.09c-1.

    Proteomic databases

    MaxQBi O74781.
    PaxDbi O74781.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC25B2.09c.1 ; SPBC25B2.09c.1:pep ; SPBC25B2.09c .
    GeneIDi 2540548.
    KEGGi spo:SPBC25B2.09c.

    Organism-specific databases

    PomBasei SPBC25B2.09c.

    Phylogenomic databases

    eggNOGi COG0018.
    HOGENOMi HOG000247211.
    KOi K01887.
    OMAi CEDRGAL.
    OrthoDBi EOG7NKKV8.
    PhylomeDBi O74781.

    Miscellaneous databases

    NextBioi 20801674.
    PROi O74781.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.30.1360.70. 1 hit.
    3.40.50.620. 1 hit.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR001278. Arg-tRNA-ligase.
    IPR005148. Arg-tRNA-synth_N.
    IPR008909. DALR_anticod-bd.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    [Graphical view ]
    PANTHERi PTHR11956. PTHR11956. 1 hit.
    Pfami PF05746. DALR_1. 1 hit.
    PF00750. tRNA-synt_1d. 1 hit.
    [Graphical view ]
    PRINTSi PR01038. TRNASYNTHARG.
    SMARTi SM01016. Arg_tRNA_synt_N. 1 hit.
    SM00836. DALR_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF55190. SSF55190. 1 hit.
    TIGRFAMsi TIGR00456. argS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "Identification of open reading frames in Schizosaccharomyces pombe cDNAs."
      Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.
      DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 220-476.
      Strain: PR745.
    3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSYRC_SCHPO
    AccessioniPrimary (citable) accession number: O74781
    Secondary accession number(s): P78751
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 10, 2002
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3