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Protein

Probable delta-1-pyrroline-5-carboxylate dehydrogenase

Gene

SPBC24C6.04

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-glutamate 5-semialdehyde + NAD+ + H2O = L-glutamate + NADH.

Pathwayi: L-proline degradation into L-glutamate

This protein is involved in step 2 of the subpathway that synthesizes L-glutamate from L-proline.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Probable delta-1-pyrroline-5-carboxylate dehydrogenase (SPBC24C6.04)
This subpathway is part of the pathway L-proline degradation into L-glutamate, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate from L-proline, the pathway L-proline degradation into L-glutamate and in Amino-acid degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei193 – 1931Transition state stabilizerBy similarity
Active sitei298 – 2981Proton acceptorPROSITE-ProRule annotation
Active sitei332 – 3321NucleophilePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Proline metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-SPO-70688. Proline catabolism.
UniPathwayiUPA00261; UER00374.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable delta-1-pyrroline-5-carboxylate dehydrogenase (EC:1.2.1.88)
Short name:
P5C dehydrogenase
Alternative name(s):
L-glutamate gamma-semialdehyde dehydrogenase
Gene namesi
ORF Names:SPBC24C6.04
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC24C6.04.
PomBaseiSPBC24C6.04.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • mitochondrion Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 548548Probable delta-1-pyrroline-5-carboxylate dehydrogenasePRO_0000056502Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei391 – 3911Phosphoserine1 Publication
Modified residuei394 – 3941Phosphoserine1 Publication
Modified residuei396 – 3961Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO74766.
PRIDEiO74766.

PTM databases

iPTMnetiO74766.

Interactioni

Protein-protein interaction databases

BioGridi276915. 20 interactions.
MINTiMINT-4680588.

Structurei

3D structure databases

ProteinModelPortaliO74766.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Phylogenomic databases

HOGENOMiHOG000271511.
InParanoidiO74766.
KOiK00294.
OMAiTDYRYPF.
OrthoDBiEOG7BCNMF.
PhylomeDBiO74766.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR005931. P5CDH/ALDH4A1.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01236. D1pyr5carbox1. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O74766-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQFAEFKLP AIKNEPPKHY GPNSADREGI VKAYKELEAE LPVTIPVIID
60 70 80 90 100
GKEVETNTIG EQRCPFEHKK VVARYHRAGA KHVEDAIEAA LRGKKVWESL
110 120 130 140 150
PFADRSAIFL KAAHLISTKY RYKLMAATMI GQGKNIWQAE IDAGMEIIDF
160 170 180 190 200
LRFNTKYASE LYASQPPENT PGVWNRMEYR PLEGFVYAIT PFNFTAIAGN
210 220 230 240 250
LAAAPLLMGN VVLMKPSDHA VLSSYIVYQI FREAGLPAGA LQFIPGDAVE
260 270 280 290 300
VSKVCFNHPE FAGLHFTGST AVFRSLWGTI GENVANGKYR TYPKIVGETG
310 320 330 340 350
GKNFHLVHSS AEIKSAVVNA VRAAFEYQGQ KCSALSRLYV SKYAWENGFR
360 370 380 390 400
DELTKQVKSL KVGAPLTDFA NFVGPVIHQA SFNKLKKVLE SAASDSEIEV
410 420 430 440 450
LAGGKADDSE GFFVEPTVLL SKNPKHDIFV NELFGPVLSV YVYEDDNLDA
460 470 480 490 500
VCDLIDTTTP YGLTGSIFAQ DRVVVRKLTD RLRNAAGNFY INDKCTGAVV
510 520 530 540
GEQPFGGARA SGTNDKAGSG MILSRFVSPR SIKDTFAYAD SVLYPSNF
Length:548
Mass (Da):60,219
Last modified:November 1, 1998 - v1
Checksum:iE14D37E32A0B4B6E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti210 – 2101N → K in BAA13891 (PubMed:9501991).Curated
Sequence conflicti264 – 2641L → F in BAA13891 (PubMed:9501991).Curated
Sequence conflicti273 – 2742FR → IC in BAA13891 (PubMed:9501991).Curated
Sequence conflicti294 – 2941K → N in BAA13891 (PubMed:9501991).Curated
Sequence conflicti502 – 5021E → G in BAA13891 (PubMed:9501991).Curated
Sequence conflicti516 – 5161K → E in BAA13891 (PubMed:9501991).Curated
Sequence conflicti526 – 5261F → Y in BAA13891 (PubMed:9501991).Curated
Sequence conflicti531 – 5311S → Y in BAA13891 (PubMed:9501991).Curated
Sequence conflicti536 – 5361F → L in BAA13891 (PubMed:9501991).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA21148.1.
D89230 mRNA. Translation: BAA13891.1.
PIRiT39968.
RefSeqiNP_595958.1. NM_001021867.2.

Genome annotation databases

EnsemblFungiiSPBC24C6.04.1; SPBC24C6.04.1:pep; SPBC24C6.04.
GeneIDi2540387.
KEGGispo:SPBC24C6.04.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA21148.1.
D89230 mRNA. Translation: BAA13891.1.
PIRiT39968.
RefSeqiNP_595958.1. NM_001021867.2.

3D structure databases

ProteinModelPortaliO74766.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276915. 20 interactions.
MINTiMINT-4680588.

PTM databases

iPTMnetiO74766.

Proteomic databases

MaxQBiO74766.
PRIDEiO74766.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC24C6.04.1; SPBC24C6.04.1:pep; SPBC24C6.04.
GeneIDi2540387.
KEGGispo:SPBC24C6.04.

Organism-specific databases

EuPathDBiFungiDB:SPBC24C6.04.
PomBaseiSPBC24C6.04.

Phylogenomic databases

HOGENOMiHOG000271511.
InParanoidiO74766.
KOiK00294.
OMAiTDYRYPF.
OrthoDBiEOG7BCNMF.
PhylomeDBiO74766.

Enzyme and pathway databases

UniPathwayiUPA00261; UER00374.
ReactomeiR-SPO-70688. Proline catabolism.

Miscellaneous databases

NextBioi20801514.
PROiO74766.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR005931. P5CDH/ALDH4A1.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01236. D1pyr5carbox1. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Identification of open reading frames in Schizosaccharomyces pombe cDNAs."
    Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.
    DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 189-548.
    Strain: PR745.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-391; SER-394 AND SER-396, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiPUT2_SCHPO
AccessioniPrimary (citable) accession number: O74766
Secondary accession number(s): P78880
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: November 1, 1998
Last modified: May 11, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.