O74766 (PUT2_SCHPO) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 78.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Probable delta-1-pyrroline-5-carboxylate dehydrogenase Short name=P5C dehydrogenase EC=1.5.1.12 | ||
| Gene names |
| ||
| Organism | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) | ||
| Taxonomic identifier | 284812 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces |
Protein attributes
| Sequence length | 548 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Catalytic activity | (S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H. |
| Pathway | |
| Sequence similarities | Belongs to the aldehyde dehydrogenase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Proline metabolism |
| Ligand | NAD |
| Molecular function | Oxidoreductase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | glutamate biosynthetic process Inferred from sequence or structural similarity. Source: GeneDB_Spombe proline biosynthetic processInferred from sequence or structural similarity. Source: GeneDB_Spombe |
| Cellular component | cytosol Inferred from direct assay. Source: GeneDB_Spombe mitochondrial matrixInferred from electronic annotation. Source: InterPro |
| Molecular function | 1-pyrroline-5-carboxylate dehydrogenase activity Inferred from sequence or structural similarity. Source: GeneDB_Spombe oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptorInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 548 | 548 | Probable delta-1-pyrroline-5-carboxylate dehydrogenase | PRO_0000056502 | |||||
Sites | |||||||||
| Active site | 298 | 1 | Proton acceptor By similarity | ||||||
| Active site | 332 | 1 | Nucleophile By similarity | ||||||
| Site | 193 | 1 | Transition state stabilizer By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 391 | 1 | Phosphoserine Ref.3 | ||||||
| Modified residue | 394 | 1 | Phosphoserine Ref.3 | ||||||
| Modified residue | 396 | 1 | Phosphoserine Ref.3 | ||||||
Experimental info | |||||||||
| Sequence conflict | 210 | 1 | N → K in BAA13891. Ref.2 | ||||||
| Sequence conflict | 264 | 1 | L → F in BAA13891. Ref.2 | ||||||
| Sequence conflict | 273 – 274 | 2 | FR → IC in BAA13891. Ref.2 | ||||||
| Sequence conflict | 294 | 1 | K → N in BAA13891. Ref.2 | ||||||
| Sequence conflict | 502 | 1 | E → G in BAA13891. Ref.2 | ||||||
| Sequence conflict | 516 | 1 | K → E in BAA13891. Ref.2 | ||||||
| Sequence conflict | 526 | 1 | F → Y in BAA13891. Ref.2 | ||||||
| Sequence conflict | 531 | 1 | S → Y in BAA13891. Ref.2 | ||||||
| Sequence conflict | 536 | 1 | F → L in BAA13891. Ref.2 | ||||||
Sequences
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References
| [1] | "The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. Nurse P.Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843. |
| [2] | "Identification of open reading frames in Schizosaccharomyces pombe cDNAs." Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H. DNA Res. 4:363-369(1997) [PubMed: 9501991] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 189-548. Strain: PR745. |
| [3] | "Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-391; SER-394 AND SER-396, MASS SPECTROMETRY. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | CU329671 Genomic DNA. Translation: CAA21148.1. D89230 mRNA. Translation: BAA13891.1. |
| PIR | T39968. |
| RefSeq | NP_595958.1. NM_001021867.1. |
3D structure databases | |
| ProteinModelPortal | O74766. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | O74766. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblFungi | SPBC24C6.04.1; SPBC24C6.04.1:pep; SPBC24C6.04. |
| GeneID | 2540387. |
| KEGG | spo:SPBC24C6.04. |
| NMPDR | fig|4896.1.peg.1824. |
Organism-specific databases | |
| GeneDB_Spombe | SPBC24C6.04. |
Phylogenomic databases | |
| eggNOG | fuNOG06074. |
| GeneTree | EFGT00050000002268. |
| HOGENOM | HBG752218. |
| OMA | HLVHPSA. |
| OrthoDB | EOG4350FG. |
Enzyme and pathway databases | |
| BioCyc | SPOM-XXX-01:SPOM-XXX-01-004021-MONOMER. |
Gene expression databases | |
| ArrayExpress | O74766. |
Family and domain databases | |
| InterPro | IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR005931. Delta1-pyrroline-5-COlate_DH-1. [Graphical view] |
| Gene3D | G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit. |
| KO | K00294. |
| Pfam | PF00171. Aldedh. 1 hit. [Graphical view] |
| SUPFAM | SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit. |
| TIGRFAMs | TIGR01236. D1pyr5carbox1. 1 hit. |
| PROSITE | PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | PUT2_SCHPO | ||||||||
| Accession | Primary (citable) accession number: O74766 Secondary accession number(s): P78880 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Schizosaccharomyces pombe Schizosaccharomyces pombe: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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