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Protein

ATP-dependent rRNA helicase spb4

Gene

spb4

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits. Binds 90S pre-ribosomal particles and dissociates from pre-60S ribosomal particles after processing of 27SB pre-rRNA. Required for the normal formation of 18S rRNA through the processing of pre-rRNAs at sites A0, A1 and A2, and the normal formation of 25S and 5.8S rRNAs through the processing of pre-rRNAs at sites C1 and C2.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi45 – 528ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent rRNA helicase spb4Curated (EC:3.6.4.13By similarity)
Gene namesi
Name:spb4By similarity
ORF Names:SPBC24C6.02
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC24C6.02.
PomBaseiSPBC24C6.02.

Subcellular locationi

  • Nucleusnucleolus By similarity

GO - Cellular componenti

  • nucleolus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 606606ATP-dependent rRNA helicase spb4PRO_0000232332Add
BLAST

Proteomic databases

MaxQBiO74764.

Interactioni

Subunit structurei

Component of pre-60S ribosomal complexes.By similarity

Structurei

3D structure databases

ProteinModelPortaliO74764.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 213182Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini246 – 400155Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili521 – 60181Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1 – 2929Q motifCuratedAdd
BLAST
Motifi161 – 1644DEAD boxCurated

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOGENOMiHOG000268803.
InParanoidiO74764.
KOiK14809.
OMAiRANVVEK.
OrthoDBiEOG7FR7R5.
PhylomeDBiO74764.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR025313. DUF4217.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF13959. DUF4217. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM01178. DUF4217. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 5 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O74764-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFQSINIDK WLKNAVAAQG FKKMTPVQAN AIPLFLKNKD LVVEAVTGSG
60 70 80 90 100
KTLAYLLPCF DKVTRRDTDE TGLGALIVAP TRELATQIFN VTKELLAYQP
110 120 130 140 150
DSLDGGKKLV ADMYIGGKGT LTNDLASFRE KNPSVVIGTP GRLNEMLSHI
160 170 180 190 200
SSKHLEILIL DEADTLIDMG FQRTLQSIIS QLPKQRRTGL FSATMNDTVS
210 220 230 240 250
SFLKIAGLRN SVRVSVTVTS KKIDTRTPSS LAIQSLVIPP IYKVQCMIHL
260 270 280 290 300
LCTIEYEKAI VFFSSCASVE YFNSLFLTYK LPFEIVALHG QQVQSNRSRN
310 320 330 340 350
FEKFKKSNKK TVLFTTDIAS RGLDIPNVDF VLQLDPPLDP KSFSHRCGRA
360 370 380 390 400
GRAGRAGVAI VLLNDGREEE YEELLRVRKV PITRIDTPIE ALDLSRLKVL
410 420 430 440 450
THELRKIVSK DRDLYDKGLR AFVSHVRAYT KHHASFIFRI KDLDLGQLAT
460 470 480 490 500
AYALLHLPKM PELKDTEISE NIFKKFDIDY ATIPYRDQVR EQARRRRLEV
510 520 530 540 550
EKTEPKKLAR PAKIKNEAWS KQKEVKEKRN TRREKRKSKK EFLKAQKNEA
560 570 580 590 600
SNNLKQEIVS KAGAQETEND DLIDEESDAL SELEEDYRQL KKSKKRKNQA

SFGFSM
Length:606
Mass (Da):68,742
Last modified:November 1, 1998 - v1
Checksum:i471BDD5BA576893B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA21146.1.
PIRiT39966.
RefSeqiNP_595956.1. NM_001021865.2.

Genome annotation databases

EnsemblFungiiSPBC24C6.02.1; SPBC24C6.02.1:pep; SPBC24C6.02.
GeneIDi2540598.
KEGGispo:SPBC24C6.02.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA21146.1.
PIRiT39966.
RefSeqiNP_595956.1. NM_001021865.2.

3D structure databases

ProteinModelPortaliO74764.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

MaxQBiO74764.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC24C6.02.1; SPBC24C6.02.1:pep; SPBC24C6.02.
GeneIDi2540598.
KEGGispo:SPBC24C6.02.

Organism-specific databases

EuPathDBiFungiDB:SPBC24C6.02.
PomBaseiSPBC24C6.02.

Phylogenomic databases

HOGENOMiHOG000268803.
InParanoidiO74764.
KOiK14809.
OMAiRANVVEK.
OrthoDBiEOG7FR7R5.
PhylomeDBiO74764.

Miscellaneous databases

PROiO74764.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR025313. DUF4217.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF13959. DUF4217. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM01178. DUF4217. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 5 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiSPB4_SCHPO
AccessioniPrimary (citable) accession number: O74764
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: November 1, 1998
Last modified: June 8, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.