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Protein

DNA primase large subunit

Gene

pri2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi291Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi369Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi386Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi428Iron-sulfur (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Nucleotidyltransferase, Transferase
Biological processDNA replication, Transcription
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

ReactomeiR-SPO-113501. Inhibition of replication initiation of damaged DNA by RB1/E2F1.
R-SPO-174411. Polymerase switching on the C-strand of the telomere.
R-SPO-174430. Telomere C-strand synthesis initiation.
R-SPO-68952. DNA replication initiation.
R-SPO-68962. Activation of the pre-replicative complex.
R-SPO-69091. Polymerase switching.
R-SPO-69166. Removal of the Flap Intermediate.
R-SPO-69183. Processive synthesis on the lagging strand.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA primase large subunit (EC:2.7.7.-)
Gene namesi
Name:pri2
Synonyms:spp2
ORF Names:SPBC17D11.06
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC17D11.06.
PomBaseiSPBC17D11.06.

Subcellular locationi

GO - Cellular componenti

  • alpha DNA polymerase:primase complex Source: PomBase
  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • nuclear replication fork Source: PomBase
  • nucleus Source: PomBase

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus, Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000467751 – 459DNA primase large subunitAdd BLAST459

Proteomic databases

MaxQBiO74761.
PRIDEiO74761.

PTM databases

SwissPalmiO74761.

Interactioni

Subunit structurei

Heterodimer of a small subunit and a large subunit.

Binary interactionsi

WithEntry#Exp.IntActNotes
pri1O142152EBI-849075,EBI-849063

Protein-protein interaction databases

BioGridi276477. 11 interactors.
IntActiO74761. 2 interactors.
MINTiMINT-4680540.
STRINGi4896.SPBC17D11.06.1.

Structurei

3D structure databases

SMRiO74761.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000212154.
InParanoidiO74761.
KOiK02685.
OMAiDCHGCPY.
OrthoDBiEOG092C27SX.
PhylomeDBiO74761.

Family and domain databases

CDDicd07322. PriL_PriS_Eukaryotic. 1 hit.
InterProiView protein in InterPro
IPR016558. DNA_primase_lsu_euk.
IPR007238. DNA_primase_lsu_euk/arc.
PfamiView protein in Pfam
PF04104. DNA_primase_lrg. 1 hit.
PIRSFiPIRSF009449. DNA_primase_large_subunit. 1 hit.

Sequencei

Sequence statusi: Complete.

O74761-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRTTKSRVV EKNQNFSSAS FESLSYPTRL NFYKKPPVSE ISIEEFETWA
60 70 80 90 100
IDRLVVLGEI ESAMLRNKTK NELNGIIKKI LDKRLPMSSN MARTVKGNSL
110 120 130 140 150
DEERRKDHYS HFILRLAFSR SDELRIRFLR AESTLFRFRF VNEETRERQA
160 170 180 190 200
FIDSLDFQWE SVSQEEKDVF YEKLQASSQR NIENESFFKV PFFKVPDLVE
210 220 230 240 250
RRAVFVHKGY AYVPFSEQVS LVVEEFEENL KKALESTAKS LPRLDEDDRL
260 270 280 290 300
LPILNHLSKG FVAPESSIVA PKSGAITAGQ VDSFVSHFPL CAKHLHEVLK
310 320 330 340 350
RDKHLRHFGR LQYGLFLKDI GLSVDEALVF WRKSFTNVTE DKFNKEYRYN
360 370 380 390 400
IRHTYGLEGN RKNYKGYNCQ QILTGPQLGP GDAHGCPYRT YSVNNLVSAL
410 420 430 440 450
ASMGIAPDSA GCREVVEAVK ARHYHIACTR VFELTHPNVG SLEESIHHPL

QYFQLSLES
Length:459
Mass (Da):53,053
Last modified:November 1, 1998 - v1
Checksum:i811172102DDFC42E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAA21077.1.
AB027983 Genomic DNA. Translation: BAA87287.1.
PIRiT39717.
RefSeqiNP_596380.1. NM_001022301.2.

Genome annotation databases

EnsemblFungiiSPBC17D11.06.1; SPBC17D11.06.1:pep; SPBC17D11.06.
GeneIDi2539933.
KEGGispo:SPBC17D11.06.

Entry informationi

Entry nameiPRI2_SCHPO
AccessioniPrimary (citable) accession number: O74761
Secondary accession number(s): Q9US83
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: November 1, 1998
Last modified: July 5, 2017
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families