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Protein

Eukaryotic peptide chain release factor GTP-binding subunit

Gene

sup35

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in translation termination. Stimulates the activity of ERF1. Binds guanine nucleotides.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi245 – 252GTPBy similarity8
Nucleotide bindingi322 – 326GTPBy similarity5
Nucleotide bindingi384 – 387GTPBy similarity4

GO - Molecular functioni

  • GDP binding Source: CAFA
  • GTPase activity Source: GO_Central
  • GTP binding Source: PomBase
  • guanyl-nucleotide exchange factor activity Source: CAFA
  • magnesium ion binding Source: CAFA
  • protein C-terminus binding Source: CAFA
  • translation release factor activity Source: InterPro

GO - Biological processi

  • cytoplasmic translational termination Source: PomBase
  • nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: InterPro

Keywordsi

Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-72764 Eukaryotic Translation Termination
R-SPO-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-SPO-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic peptide chain release factor GTP-binding subunit
Alternative name(s):
ERF-3
Short name:
ERF3
ERF2
Polypeptide release factor 3
Translation release factor 3
Gene namesi
Name:sup35
ORF Names:SPCC584.04
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC584.04
PomBaseiSPCC584.04 sup35

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000914881 – 662Eukaryotic peptide chain release factor GTP-binding subunitAdd BLAST662

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei182Phosphothreonine1 Publication1
Modified residuei539Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO74718
PaxDbiO74718
PRIDEiO74718

PTM databases

iPTMnetiO74718

Interactioni

GO - Molecular functioni

  • guanyl-nucleotide exchange factor activity Source: CAFA
  • protein C-terminus binding Source: CAFA

Protein-protein interaction databases

BioGridi276042, 6 interactors
DIPiDIP-59469N
IntActiO74718, 1 interactor
STRINGi4896.SPCC584.04.1

Structurei

Secondary structure

1662
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni221 – 224Combined sources4
Beta strandi226 – 228Combined sources3
Helixi230 – 233Combined sources4
Beta strandi237 – 245Combined sources9
Helixi247 – 249Combined sources3
Helixi251 – 261Combined sources11
Helixi267 – 276Combined sources10
Beta strandi310 – 312Combined sources3
Beta strandi314 – 320Combined sources7
Beta strandi341 – 348Combined sources8
Helixi353 – 356Combined sources4
Beta strandi359 – 361Combined sources3
Helixi364 – 373Combined sources10
Beta strandi377 – 384Combined sources8
Beta strandi386 – 388Combined sources3
Turni389 – 393Combined sources5
Helixi395 – 413Combined sources19
Helixi417 – 420Combined sources4
Beta strandi421 – 425Combined sources5
Turni428 – 431Combined sources4
Beta strandi434 – 436Combined sources3
Turni440 – 442Combined sources3
Helixi451 – 457Combined sources7
Helixi462 – 465Combined sources4
Beta strandi470 – 472Combined sources3
Beta strandi475 – 487Combined sources13
Beta strandi490 – 494Combined sources5
Beta strandi497 – 502Combined sources6
Turni503 – 505Combined sources3
Beta strandi506 – 514Combined sources9
Beta strandi520 – 525Combined sources6
Beta strandi529 – 536Combined sources8
Beta strandi546 – 548Combined sources3
Beta strandi550 – 552Combined sources3
Beta strandi556 – 566Combined sources11
Beta strandi573 – 575Combined sources3
Beta strandi579 – 585Combined sources7
Beta strandi590 – 594Combined sources5
Beta strandi601 – 603Combined sources3
Beta strandi621 – 630Combined sources10
Turni634 – 636Combined sources3
Helixi638 – 641Combined sources4
Beta strandi642 – 646Combined sources5
Beta strandi648 – 650Combined sources3
Beta strandi652 – 661Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R5BX-ray2.35A196-662[»]
1R5NX-ray2.90A196-662[»]
1R5OX-ray3.20A196-662[»]
3E20X-ray3.50A/D/E/J467-662[»]
DisProtiDP00396
ProteinModelPortaliO74718
SMRiO74718
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO74718

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini236 – 464tr-type GPROSITE-ProRule annotationAdd BLAST229

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni245 – 252G1PROSITE-ProRule annotation8
Regioni301 – 305G2PROSITE-ProRule annotation5
Regioni322 – 325G3PROSITE-ProRule annotation4
Regioni384 – 387G4PROSITE-ProRule annotation4
Regioni427 – 429G5PROSITE-ProRule annotation3

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. ERF3 subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000229291
InParanoidiO74718
KOiK03267
OMAiWEHSKEV
OrthoDBiEOG092C2HV8
PhylomeDBiO74718

Family and domain databases

InterProiView protein in InterPro
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR003285 Sup35
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
IPR004160 Transl_elong_EFTu/EF1A_C
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03143 GTP_EFTU_D3, 1 hit
PRINTSiPR00315 ELONGATNFCT
PR01343 YEASTERF
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

O74718-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASNQPNNGE QDEQLAKQTS KLSMSAKAPT FTPKAAPFIP SFQRPGFVPV
60 70 80 90 100
NNIAGGYPYA QYTGQGQNSN SPHPTKSYQQ YYQKPTGNTV DEDKSRVPDF
110 120 130 140 150
SKKKSFVPPK PAIPKGKVLS LGGNTSAPKS TKPISISLGG TKAPTTTKPA
160 170 180 190 200
APAAQSKTET PAPKVTSEST KKETAAPPPQ ETPTKSADAE LAKTPSAPAA
210 220 230 240 250
ALKKAAEAAE PATVTEDATD LQNEVDQELL KDMYGKEHVN IVFIGHVDAG
260 270 280 290 300
KSTLGGNILF LTGMVDKRTM EKIEREAKEA GKESWYLSWA LDSTSEEREK
310 320 330 340 350
GKTVEVGRAY FETEHRRFSL LDAPGHKGYV TNMINGASQA DIGVLVISAR
360 370 380 390 400
RGEFEAGFER GGQTREHAVL ARTQGINHLV VVINKMDEPS VQWSEERYKE
410 420 430 440 450
CVDKLSMFLR RVAGYNSKTD VKYMPVSAYT GQNVKDRVDS SVCPWYQGPS
460 470 480 490 500
LLEYLDSMTH LERKVNAPFI MPIASKYKDL GTILEGKIEA GSIKKNSNVL
510 520 530 540 550
VMPINQTLEV TAIYDEADEE ISSSICGDQV RLRVRGDDSD VQTGYVLTST
560 570 580 590 600
KNPVHATTRF IAQIAILELP SILTTGYSCV MHIHTAVEEV SFAKLLHKLD
610 620 630 640 650
KTNRKSKKPP MFATKGMKII AELETQTPVC MERFEDYQYM GRFTLRDQGT
660
TVAVGKVVKI LD
Length:662
Mass (Da):72,556
Last modified:May 30, 2000 - v2
Checksum:i54AE41EA6AF6168A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti79 – 80QQ → LL in BAA33530 (PubMed:9701287).Curated2
Sequence conflicti388E → D in BAA13868 (PubMed:9501991).Curated1
Sequence conflicti397 – 398RY → SN in BAA13868 (PubMed:9501991).Curated2
Sequence conflicti589E → G in BAA33530 (PubMed:9701287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D79214 Genomic DNA Translation: BAA33530.1
CU329672 Genomic DNA Translation: CAA21821.1
D89207 mRNA Translation: BAA13868.1
PIRiT41442
T43011
T51948
RefSeqiNP_588225.1, NM_001023215.2

Genome annotation databases

EnsemblFungiiSPCC584.04.1; SPCC584.04.1:pep; SPCC584.04
GeneIDi2539479
KEGGispo:SPCC584.04

Similar proteinsi

Entry informationi

Entry nameiERF3_SCHPO
AccessioniPrimary (citable) accession number: O74718
Secondary accession number(s): P78857
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 30, 2000
Last modified: March 28, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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