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Protein

Eukaryotic peptide chain release factor GTP-binding subunit

Gene

sup35

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in translation termination. Stimulates the activity of ERF1. Binds guanine nucleotides.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi245 – 2528GTPBy similarity
Nucleotide bindingi322 – 3265GTPBy similarity
Nucleotide bindingi384 – 3874GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • translation release factor activity Source: PomBase

GO - Biological processi

  • cytoplasmic translational termination Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SPO-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic peptide chain release factor GTP-binding subunit
Alternative name(s):
ERF-3
Short name:
ERF3
ERF2
Polypeptide release factor 3
Translation release factor 3
Gene namesi
Name:sup35
ORF Names:SPCC584.04
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC584.04.
PomBaseiSPCC584.04. sup35.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • translation release factor complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 662662Eukaryotic peptide chain release factor GTP-binding subunitPRO_0000091488Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei182 – 1821Phosphothreonine1 Publication
Modified residuei539 – 5391Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO74718.

PTM databases

iPTMnetiO74718.

Interactioni

Protein-protein interaction databases

BioGridi276042. 5 interactions.
DIPiDIP-59469N.
MINTiMINT-4680246.

Structurei

Secondary structure

1
662
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni221 – 2244Combined sources
Beta strandi226 – 2283Combined sources
Helixi230 – 2334Combined sources
Beta strandi237 – 2459Combined sources
Helixi247 – 2493Combined sources
Helixi251 – 26111Combined sources
Helixi267 – 27610Combined sources
Beta strandi310 – 3123Combined sources
Beta strandi314 – 3207Combined sources
Beta strandi341 – 3488Combined sources
Helixi353 – 3564Combined sources
Beta strandi359 – 3613Combined sources
Helixi364 – 37310Combined sources
Beta strandi377 – 3848Combined sources
Beta strandi386 – 3883Combined sources
Turni389 – 3935Combined sources
Helixi395 – 41319Combined sources
Helixi417 – 4204Combined sources
Beta strandi421 – 4255Combined sources
Turni428 – 4314Combined sources
Beta strandi434 – 4363Combined sources
Turni440 – 4423Combined sources
Helixi451 – 4577Combined sources
Helixi462 – 4654Combined sources
Beta strandi470 – 4723Combined sources
Beta strandi475 – 48713Combined sources
Beta strandi490 – 4945Combined sources
Beta strandi497 – 5026Combined sources
Turni503 – 5053Combined sources
Beta strandi506 – 5149Combined sources
Beta strandi520 – 5256Combined sources
Beta strandi529 – 5368Combined sources
Beta strandi546 – 5483Combined sources
Beta strandi550 – 5523Combined sources
Beta strandi556 – 56611Combined sources
Beta strandi573 – 5753Combined sources
Beta strandi579 – 5857Combined sources
Beta strandi590 – 5945Combined sources
Beta strandi601 – 6033Combined sources
Beta strandi621 – 63010Combined sources
Turni634 – 6363Combined sources
Helixi638 – 6414Combined sources
Beta strandi642 – 6465Combined sources
Beta strandi648 – 6503Combined sources
Beta strandi652 – 66110Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R5BX-ray2.35A196-662[»]
1R5NX-ray2.90A196-662[»]
1R5OX-ray3.20A196-662[»]
3E20X-ray3.50A/D/E/J467-662[»]
DisProtiDP00396.
ProteinModelPortaliO74718.
SMRiO74718. Positions 215-662.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO74718.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini236 – 464229tr-type GPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni245 – 2528G1PROSITE-ProRule annotation
Regioni301 – 3055G2PROSITE-ProRule annotation
Regioni322 – 3254G3PROSITE-ProRule annotation
Regioni384 – 3874G4PROSITE-ProRule annotation
Regioni427 – 4293G5PROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. ERF3 subfamily.PROSITE-ProRule annotation
Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000229291.
InParanoidiO74718.
KOiK03267.
OMAiKLNANAF.
OrthoDBiEOG715QCW.
PhylomeDBiO74718.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR003285. Sup35.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
PR01343. YEASTERF.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O74718-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASNQPNNGE QDEQLAKQTS KLSMSAKAPT FTPKAAPFIP SFQRPGFVPV
60 70 80 90 100
NNIAGGYPYA QYTGQGQNSN SPHPTKSYQQ YYQKPTGNTV DEDKSRVPDF
110 120 130 140 150
SKKKSFVPPK PAIPKGKVLS LGGNTSAPKS TKPISISLGG TKAPTTTKPA
160 170 180 190 200
APAAQSKTET PAPKVTSEST KKETAAPPPQ ETPTKSADAE LAKTPSAPAA
210 220 230 240 250
ALKKAAEAAE PATVTEDATD LQNEVDQELL KDMYGKEHVN IVFIGHVDAG
260 270 280 290 300
KSTLGGNILF LTGMVDKRTM EKIEREAKEA GKESWYLSWA LDSTSEEREK
310 320 330 340 350
GKTVEVGRAY FETEHRRFSL LDAPGHKGYV TNMINGASQA DIGVLVISAR
360 370 380 390 400
RGEFEAGFER GGQTREHAVL ARTQGINHLV VVINKMDEPS VQWSEERYKE
410 420 430 440 450
CVDKLSMFLR RVAGYNSKTD VKYMPVSAYT GQNVKDRVDS SVCPWYQGPS
460 470 480 490 500
LLEYLDSMTH LERKVNAPFI MPIASKYKDL GTILEGKIEA GSIKKNSNVL
510 520 530 540 550
VMPINQTLEV TAIYDEADEE ISSSICGDQV RLRVRGDDSD VQTGYVLTST
560 570 580 590 600
KNPVHATTRF IAQIAILELP SILTTGYSCV MHIHTAVEEV SFAKLLHKLD
610 620 630 640 650
KTNRKSKKPP MFATKGMKII AELETQTPVC MERFEDYQYM GRFTLRDQGT
660
TVAVGKVVKI LD
Length:662
Mass (Da):72,556
Last modified:May 30, 2000 - v2
Checksum:i54AE41EA6AF6168A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti79 – 802QQ → LL in BAA33530 (PubMed:9701287).Curated
Sequence conflicti388 – 3881E → D in BAA13868 (PubMed:9501991).Curated
Sequence conflicti397 – 3982RY → SN in BAA13868 (PubMed:9501991).Curated
Sequence conflicti589 – 5891E → G in BAA33530 (PubMed:9701287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D79214 Genomic DNA. Translation: BAA33530.1.
CU329672 Genomic DNA. Translation: CAA21821.1.
D89207 mRNA. Translation: BAA13868.1.
PIRiT41442.
T43011.
T51948.
RefSeqiNP_588225.1. NM_001023215.2.

Genome annotation databases

EnsemblFungiiSPCC584.04.1; SPCC584.04.1:pep; SPCC584.04.
GeneIDi2539479.
KEGGispo:SPCC584.04.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D79214 Genomic DNA. Translation: BAA33530.1.
CU329672 Genomic DNA. Translation: CAA21821.1.
D89207 mRNA. Translation: BAA13868.1.
PIRiT41442.
T43011.
T51948.
RefSeqiNP_588225.1. NM_001023215.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R5BX-ray2.35A196-662[»]
1R5NX-ray2.90A196-662[»]
1R5OX-ray3.20A196-662[»]
3E20X-ray3.50A/D/E/J467-662[»]
DisProtiDP00396.
ProteinModelPortaliO74718.
SMRiO74718. Positions 215-662.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276042. 5 interactions.
DIPiDIP-59469N.
MINTiMINT-4680246.

PTM databases

iPTMnetiO74718.

Proteomic databases

MaxQBiO74718.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC584.04.1; SPCC584.04.1:pep; SPCC584.04.
GeneIDi2539479.
KEGGispo:SPCC584.04.

Organism-specific databases

EuPathDBiFungiDB:SPCC584.04.
PomBaseiSPCC584.04. sup35.

Phylogenomic databases

HOGENOMiHOG000229291.
InParanoidiO74718.
KOiK03267.
OMAiKLNANAF.
OrthoDBiEOG715QCW.
PhylomeDBiO74718.

Enzyme and pathway databases

ReactomeiR-SPO-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SPO-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

EvolutionaryTraceiO74718.
NextBioi20800641.
PROiO74718.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR003285. Sup35.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
PR01343. YEASTERF.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The stretch of C-terminal acidic amino acids of translational release factor eRF1 is a primary binding site for eRF3 of fission yeast."
    Ito K., Ebihara K., Nakamura Y.
    RNA 4:958-972(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: JY333.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Identification of open reading frames in Schizosaccharomyces pombe cDNAs."
    Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.
    DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 383-662.
    Strain: PR745.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-182 AND SER-539, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiERF3_SCHPO
AccessioniPrimary (citable) accession number: O74718
Secondary accession number(s): P78857
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 30, 2000
Last modified: May 11, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.