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Protein

Eukaryotic peptide chain release factor GTP-binding subunit

Gene

sup35

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in translation termination. Stimulates the activity of ERF1. Binds guanine nucleotides.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi245 – 252GTPBy similarity8
Nucleotide bindingi322 – 326GTPBy similarity5
Nucleotide bindingi384 – 387GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: GO_Central
  • GTP binding Source: UniProtKB-KW
  • translation release factor activity Source: PomBase

GO - Biological processi

  • cytoplasmic translational termination Source: PomBase
  • nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-72764. Eukaryotic Translation Termination.
R-SPO-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SPO-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic peptide chain release factor GTP-binding subunit
Alternative name(s):
ERF-3
Short name:
ERF3
ERF2
Polypeptide release factor 3
Translation release factor 3
Gene namesi
Name:sup35
ORF Names:SPCC584.04
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC584.04.
PomBaseiSPCC584.04. sup35.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • translation release factor complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000914881 – 662Eukaryotic peptide chain release factor GTP-binding subunitAdd BLAST662

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei182Phosphothreonine1 Publication1
Modified residuei539Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO74718.
PRIDEiO74718.

PTM databases

iPTMnetiO74718.

Interactioni

Protein-protein interaction databases

BioGridi276042. 5 interactors.
DIPiDIP-59469N.
MINTiMINT-4680246.

Structurei

Secondary structure

1662
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni221 – 224Combined sources4
Beta strandi226 – 228Combined sources3
Helixi230 – 233Combined sources4
Beta strandi237 – 245Combined sources9
Helixi247 – 249Combined sources3
Helixi251 – 261Combined sources11
Helixi267 – 276Combined sources10
Beta strandi310 – 312Combined sources3
Beta strandi314 – 320Combined sources7
Beta strandi341 – 348Combined sources8
Helixi353 – 356Combined sources4
Beta strandi359 – 361Combined sources3
Helixi364 – 373Combined sources10
Beta strandi377 – 384Combined sources8
Beta strandi386 – 388Combined sources3
Turni389 – 393Combined sources5
Helixi395 – 413Combined sources19
Helixi417 – 420Combined sources4
Beta strandi421 – 425Combined sources5
Turni428 – 431Combined sources4
Beta strandi434 – 436Combined sources3
Turni440 – 442Combined sources3
Helixi451 – 457Combined sources7
Helixi462 – 465Combined sources4
Beta strandi470 – 472Combined sources3
Beta strandi475 – 487Combined sources13
Beta strandi490 – 494Combined sources5
Beta strandi497 – 502Combined sources6
Turni503 – 505Combined sources3
Beta strandi506 – 514Combined sources9
Beta strandi520 – 525Combined sources6
Beta strandi529 – 536Combined sources8
Beta strandi546 – 548Combined sources3
Beta strandi550 – 552Combined sources3
Beta strandi556 – 566Combined sources11
Beta strandi573 – 575Combined sources3
Beta strandi579 – 585Combined sources7
Beta strandi590 – 594Combined sources5
Beta strandi601 – 603Combined sources3
Beta strandi621 – 630Combined sources10
Turni634 – 636Combined sources3
Helixi638 – 641Combined sources4
Beta strandi642 – 646Combined sources5
Beta strandi648 – 650Combined sources3
Beta strandi652 – 661Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R5BX-ray2.35A196-662[»]
1R5NX-ray2.90A196-662[»]
1R5OX-ray3.20A196-662[»]
3E20X-ray3.50A/D/E/J467-662[»]
DisProtiDP00396.
ProteinModelPortaliO74718.
SMRiO74718.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO74718.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini236 – 464tr-type GPROSITE-ProRule annotationAdd BLAST229

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni245 – 252G1PROSITE-ProRule annotation8
Regioni301 – 305G2PROSITE-ProRule annotation5
Regioni322 – 325G3PROSITE-ProRule annotation4
Regioni384 – 387G4PROSITE-ProRule annotation4
Regioni427 – 429G5PROSITE-ProRule annotation3

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. ERF3 subfamily.PROSITE-ProRule annotation
Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000229291.
InParanoidiO74718.
KOiK03267.
OMAiKLNANAF.
OrthoDBiEOG092C2HV8.
PhylomeDBiO74718.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR003285. Sup35.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
PR01343. YEASTERF.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O74718-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASNQPNNGE QDEQLAKQTS KLSMSAKAPT FTPKAAPFIP SFQRPGFVPV
60 70 80 90 100
NNIAGGYPYA QYTGQGQNSN SPHPTKSYQQ YYQKPTGNTV DEDKSRVPDF
110 120 130 140 150
SKKKSFVPPK PAIPKGKVLS LGGNTSAPKS TKPISISLGG TKAPTTTKPA
160 170 180 190 200
APAAQSKTET PAPKVTSEST KKETAAPPPQ ETPTKSADAE LAKTPSAPAA
210 220 230 240 250
ALKKAAEAAE PATVTEDATD LQNEVDQELL KDMYGKEHVN IVFIGHVDAG
260 270 280 290 300
KSTLGGNILF LTGMVDKRTM EKIEREAKEA GKESWYLSWA LDSTSEEREK
310 320 330 340 350
GKTVEVGRAY FETEHRRFSL LDAPGHKGYV TNMINGASQA DIGVLVISAR
360 370 380 390 400
RGEFEAGFER GGQTREHAVL ARTQGINHLV VVINKMDEPS VQWSEERYKE
410 420 430 440 450
CVDKLSMFLR RVAGYNSKTD VKYMPVSAYT GQNVKDRVDS SVCPWYQGPS
460 470 480 490 500
LLEYLDSMTH LERKVNAPFI MPIASKYKDL GTILEGKIEA GSIKKNSNVL
510 520 530 540 550
VMPINQTLEV TAIYDEADEE ISSSICGDQV RLRVRGDDSD VQTGYVLTST
560 570 580 590 600
KNPVHATTRF IAQIAILELP SILTTGYSCV MHIHTAVEEV SFAKLLHKLD
610 620 630 640 650
KTNRKSKKPP MFATKGMKII AELETQTPVC MERFEDYQYM GRFTLRDQGT
660
TVAVGKVVKI LD
Length:662
Mass (Da):72,556
Last modified:May 30, 2000 - v2
Checksum:i54AE41EA6AF6168A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti79 – 80QQ → LL in BAA33530 (PubMed:9701287).Curated2
Sequence conflicti388E → D in BAA13868 (PubMed:9501991).Curated1
Sequence conflicti397 – 398RY → SN in BAA13868 (PubMed:9501991).Curated2
Sequence conflicti589E → G in BAA33530 (PubMed:9701287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D79214 Genomic DNA. Translation: BAA33530.1.
CU329672 Genomic DNA. Translation: CAA21821.1.
D89207 mRNA. Translation: BAA13868.1.
PIRiT41442.
T43011.
T51948.
RefSeqiNP_588225.1. NM_001023215.2.

Genome annotation databases

EnsemblFungiiSPCC584.04.1; SPCC584.04.1:pep; SPCC584.04.
GeneIDi2539479.
KEGGispo:SPCC584.04.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D79214 Genomic DNA. Translation: BAA33530.1.
CU329672 Genomic DNA. Translation: CAA21821.1.
D89207 mRNA. Translation: BAA13868.1.
PIRiT41442.
T43011.
T51948.
RefSeqiNP_588225.1. NM_001023215.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R5BX-ray2.35A196-662[»]
1R5NX-ray2.90A196-662[»]
1R5OX-ray3.20A196-662[»]
3E20X-ray3.50A/D/E/J467-662[»]
DisProtiDP00396.
ProteinModelPortaliO74718.
SMRiO74718.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276042. 5 interactors.
DIPiDIP-59469N.
MINTiMINT-4680246.

PTM databases

iPTMnetiO74718.

Proteomic databases

MaxQBiO74718.
PRIDEiO74718.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC584.04.1; SPCC584.04.1:pep; SPCC584.04.
GeneIDi2539479.
KEGGispo:SPCC584.04.

Organism-specific databases

EuPathDBiFungiDB:SPCC584.04.
PomBaseiSPCC584.04. sup35.

Phylogenomic databases

HOGENOMiHOG000229291.
InParanoidiO74718.
KOiK03267.
OMAiKLNANAF.
OrthoDBiEOG092C2HV8.
PhylomeDBiO74718.

Enzyme and pathway databases

ReactomeiR-SPO-72764. Eukaryotic Translation Termination.
R-SPO-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SPO-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

EvolutionaryTraceiO74718.
PROiO74718.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR003285. Sup35.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
PR01343. YEASTERF.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiERF3_SCHPO
AccessioniPrimary (citable) accession number: O74718
Secondary accession number(s): P78857
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 30, 2000
Last modified: November 2, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.