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Protein

Endo-1,4-beta-xylanase 2

Gene

xyl2

Organism
Claviceps purpurea (Ergot fungus) (Sphacelia segetum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose.By similarity

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.

Pathwayi: xylan degradation

This protein is involved in the pathway xylan degradation, which is part of Glycan degradation.
View all proteins of this organism that are known to be involved in the pathway xylan degradation and in Glycan degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei157Proton donorBy similarity1
Active sitei262NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Polysaccharide degradation, Xylan degradation

Enzyme and pathway databases

UniPathwayiUPA00114.

Protein family/group databases

CAZyiGH10. Glycoside Hydrolase Family 10.
mycoCLAPiXYN10B_CLAPU.

Names & Taxonomyi

Protein namesi
Recommended name:
Endo-1,4-beta-xylanase 2 (EC:3.2.1.8)
Short name:
Xylanase 2
Alternative name(s):
1,4-beta-D-xylan xylanohydrolase 2
Gene namesi
Name:xyl2
OrganismiClaviceps purpurea (Ergot fungus) (Sphacelia segetum)
Taxonomic identifieri5111 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesClavicipitaceaeClaviceps

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000042975119 – 325Endo-1,4-beta-xylanase 2Add BLAST307

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi28N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi280 ↔ 286By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Inductioni

Expressed throughout the entire infection process during in infection of rye tissue.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO74717.
SMRiO74717.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 325GH10PROSITE-ProRule annotationAdd BLAST300

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiView protein in InterPro
IPR001000. GH10.
IPR031158. GH10_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
PfamiView protein in Pfam
PF00331. Glyco_hydro_10. 1 hit.
PRINTSiPR00134. GLHYDRLASE10.
SMARTiView protein in SMART
SM00633. Glyco_10. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiView protein in PROSITE
PS00591. GH10_1. 1 hit.
PS51760. GH10_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O74717-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLYTSIFAAA MAASGAMAAP TTSHGASNCT TLDSFFKSHG KLYWGTAADK
60 70 80 90 100
NTLMKPGVAD FIAKEFGQVT PENSMKFDAT EPSRGQFHFD AADYLVDYAE
110 120 130 140 150
KHDLLIRGHT FLWWSQMPAW VKAIKDKDTL IDVIQTHIST VAGRYKGKIY
160 170 180 190 200
AWDVVNEIFE QDGSFRKTVY YNLLGEDYVR IAFEAAHKAD PKAKLYINDF
210 220 230 240 250
NLDDPNAAKL KAMIKYVTKW RAAGWPVHGI GSQSHLFAGM GEKSAAAIKM
260 270 280 290 300
LGAAADEVAI TELDITGAPQ ADYEAVTKGC IDVKNCVGIT SWGARDTDSW
310 320
LASKSPLLFD GNFKPKAAVK AIMAI
Length:325
Mass (Da):35,603
Last modified:November 1, 1998 - v1
Checksum:iCB30E344A8A9D52A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y16970 Genomic DNA. Translation: CAA76571.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y16970 Genomic DNA. Translation: CAA76571.1.

3D structure databases

ProteinModelPortaliO74717.
SMRiO74717.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH10. Glycoside Hydrolase Family 10.
mycoCLAPiXYN10B_CLAPU.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00114.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiView protein in InterPro
IPR001000. GH10.
IPR031158. GH10_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
PfamiView protein in Pfam
PF00331. Glyco_hydro_10. 1 hit.
PRINTSiPR00134. GLHYDRLASE10.
SMARTiView protein in SMART
SM00633. Glyco_10. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiView protein in PROSITE
PS00591. GH10_1. 1 hit.
PS51760. GH10_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiXYN2_CLAPU
AccessioniPrimary (citable) accession number: O74717
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 9, 2014
Last sequence update: November 1, 1998
Last modified: February 15, 2017
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.