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Protein

Histidine biosynthesis trifunctional protein

Gene

HIS4

Organism
Candida albicans (Yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

1-(5-phospho-beta-D-ribosyl)-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide.
1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-AMP + diphosphate.
L-histidinol + H2O + 2 NAD+ = L-histidine + 2 NADH.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Pathwayi: L-histidine biosynthesis

This protein is involved in step 2, 3 and 9 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Histidine biosynthesis trifunctional protein (HIS4)
  3. Histidine biosynthesis trifunctional protein (HIS4)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (HIS6)
  5. Imidazole glycerol phosphate synthase hisHF (HIS7)
  6. Imidazoleglycerol-phosphate dehydratase (HIS3)
  7. no protein annotated in this organism
  8. no protein annotated in this organism
  9. Histidine biosynthesis trifunctional protein (HIS4)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi660ZincBy similarity1
Metal bindingi663ZincBy similarity1
Active sitei729By similarity1
Active sitei730By similarity1
Metal bindingi764ZincBy similarity1
Metal bindingi823ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Oxidoreductase
Biological processAmino-acid biosynthesis, Histidine biosynthesis
LigandATP-binding, Metal-binding, NAD, Nucleotide-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00031; UER00007
UPA00031; UER00008
UPA00031; UER00014

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine biosynthesis trifunctional protein
Including the following 3 domains:
Phosphoribosyl-AMP cyclohydrolase (EC:3.5.4.19)
Phosphoribosyl-ATP pyrophosphohydrolase (EC:3.6.1.31)
Histidinol dehydrogenase (EC:1.1.1.23)
Short name:
HDH
Gene namesi
Name:HIS4
OrganismiCandida albicans (Yeast)
Taxonomic identifieri5476 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001359091 – 838Histidine biosynthesis trifunctional proteinAdd BLAST838

Proteomic databases

PRIDEiO74712

Structurei

3D structure databases

ProteinModelPortaliO74712
SMRiO74712
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 271Phosphoribosyl-AMP cyclohydrolaseAdd BLAST271
Regioni272 – 360Phosphoribosyl-ATP pyrophosphohydrolaseAdd BLAST89
Regioni361 – 838Histidinol dehydrogenaseAdd BLAST478

Sequence similaritiesi

In the C-terminal section; belongs to the histidinol dehydrogenase family.Curated

Family and domain databases

CDDicd06572 Histidinol_dh, 1 hit
Gene3Di3.10.20.400, 1 hit
HAMAPiMF_01024 HisD, 1 hit
InterProiView protein in InterPro
IPR016161 Ald_DH/histidinol_DH
IPR008179 HisE
IPR016298 Histidine_synth_trifunct
IPR001692 Histidinol_DH_CS
IPR012131 Hstdl_DH
IPR021130 PRib-ATP_PPHydrolase-like
IPR002496 PRib_AMP_CycHydrolase_dom
IPR038019 PRib_AMP_CycHydrolase_sf
PANTHERiPTHR21256 PTHR21256, 1 hit
PfamiView protein in Pfam
PF00815 Histidinol_dh, 1 hit
PF01502 PRA-CH, 1 hit
PF01503 PRA-PH, 1 hit
PIRSFiPIRSF001257 His_trifunctional, 1 hit
PRINTSiPR00083 HOLDHDRGNASE
ProDomiView protein in ProDom or Entries sharing at least one domain
PD002610 PRA_CycHdrlase, 1 hit
SUPFAMiSSF141734 SSF141734, 1 hit
SSF53720 SSF53720, 1 hit
TIGRFAMsiTIGR00069 hisD, 1 hit
TIGR03188 histidine_hisI, 1 hit
PROSITEiView protein in PROSITE
PS00611 HISOL_DEHYDROGENASE, 1 hit

Sequencei

Sequence statusi: Complete.

O74712-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIFPILPVIS SPEDKQSIDE FSLVGQVLFP IESVSPKKHF IHQFPHDLDI
60 70 80 90 100
FVNAIDNATT DQIVELLNVG IKQVFVNEKQ YHDAIEAGSP SSRFVVAVDV
110 120 130 140 150
PSTELLTSEA SFVTSKPFSE SDLKKYNANE NRVIYIESNF TQDGAIELAK
160 170 180 190 200
NYVPVIPSTK LTVKREEENK ISISAVFVST LTTDRPDGLY TTLITTPSPS
210 220 230 240 250
YTALGIVYSS KDSIIAAIEE KVGVYQSRKR RDELWYKGKT SGATQKLVKL
260 270 280 290 300
SKDCDSDVIQ FMVEPRTGYG FCHRETKFTC FGDDIADSPA RGLPKLDSTL
310 320 330 340 350
QDRLENAPEG SYTKRLFDDE KLLIAKLKEE LDELIEAKSK EEIAWECADL
360 370 380 390 400
VYFAMVWCIK HGVRLADIEK NLDVKSLKVS RRKGDAKPQY QEAPVNSSYK
410 420 430 440 450
LEIVSVDDAA AVERAMTRPV QKTADIMKLV LPIIEKVKSD GDKALIELTS
460 470 480 490 500
KFDGVKLDAP VLQAPFPADL MDISEEMKAA IDLSMQNIEK FHAAQLPKEK
510 520 530 540 550
VMTVETSPGV YCSRFAKPIE NVGLYVPGGT AVLPSTAMML GVPAKVAGCK
560 570 580 590 600
NIIVASPPSR ATGKLTPEVV YVAHKLGAKC IVMAGGAQAV TAMAYGTESV
610 620 630 640 650
LKCDKILGPG NQFVTAAKMY VQNDTQALCS IDMPAGPSEV LVIADSNADA
660 670 680 690 700
DFVASDLLSQ AEHGVDSQVI LIGVGLSDEK LNEFQAAVER QAKVLPRKDI
710 720 730 740 750
VAKCLAHSYI LLAKTYKEAF DLSNQYAPEH LILQIDDAPS YVPDSIENAG
760 770 780 790 800
SVFVGALSPE SCGDYSSGTN HTLPTYGYAR QYSGVNTATF QKFITSQEVT
810 820 830
EKGLQNIGKA VMELARVEGL EAHRRAVEIR MERMAETK
Length:838
Mass (Da):91,836
Last modified:November 1, 1998 - v1
Checksum:iBFA0E97E9CA62C03
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ003115 Genomic DNA Translation: CAA05871.1

Similar proteinsi

Entry informationi

Entry nameiHIS2_CANAX
AccessioniPrimary (citable) accession number: O74712
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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