O74711 (PEX5_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peroxisomal targeting signal receptor
Short name=PTS1 receptor
Short name=PTS1R

Alternative name(s):
Peroxin-5

Gene names

Name:	PEX5
ORF Names:	CaO19.13085, CaO19.5640

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida › Candida albicans

Protein attributes

Sequence length	592 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Binds to the C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type) and plays an essential role in peroxisomal protein import By similarity.
Subcellular location	Cytoplasm By similarity. Peroxisome membrane; Peripheral membrane protein By similarity. Note: Its distribution appears to be dynamic. It is probably a cycling receptor found mainly in the cytoplasm and as well associated to the peroxisomal membrane By similarity.
Post-translational modification	Ubiquitination at Cys-10 is UBC4-independent but requires the presence of PEX4. Ubiquitination at Lys-22 is UBC4-dependent By similarity.
Sequence similarities	Belongs to the peroxisomal targeting signal receptor family. Contains 5 TPR repeats.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Membrane Peroxisome
Domain	Repeat TPR repeat
PTM	Isopeptide bond Thioester bond Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fatty acid beta-oxidation Inferred from mutant phenotype PubMed 16963628. Source: CGD protein import into peroxisome matrix, docking Inferred from sequence orthology PubMed 16978390. Source: CGD
Cellular_component	cytosol Inferred from sequence orthology PubMed 16978390. Source: CGD peroxisomal membrane Inferred from sequence orthology PubMed 16978390. Source: CGD
Molecular_function	peroxisome matrix targeting signal-1 binding Inferred from sequence orthology PubMed 16978390. Source: CGD protein binding, bridging Inferred from sequence orthology PubMed 16978390. Source: CGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 592	592	Peroxisomal targeting signal receptor		PRO_0000106308
Regions
	Repeat	295 – 329	35	TPR 1
	Repeat	330 – 363	34	TPR 2
	Repeat	440 – 473	34	TPR 3
	Repeat	475 – 507	33	TPR 4
	Repeat	509 – 541	33	TPR 5
Amino acid modifications
	Cross-link	10		Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in ubiquitin) By similarity
	Cross-link	22		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O74711 [UniParc]. Last modified January 24, 2006. Version 2. Checksum: FA5B271F220B38E6	FASTA	592	67,324
	10 20 30 40 50 60 MSFVGGGSEC SVNGNAVAQF NKHTQQDRSL QQQVANQHGN VAQNQGFKKD NLMNVRDRAN 70 80 90 100 110 120 LDQFMNNGAP QNSFQFQPMR HELNTIQNQP NAIHQQQSNW SQDFVAQSPS AQITTPIAKT 130 140 150 160 170 180 GSPVNAQWAS EFSQAPATQQ HNQRPGQFGP RLGGYRPMMG MSMAYQSQPQ QQQQQQHNQE 190 200 210 220 230 240 PQVDWENQFK EIEELTNKAE EVEEIQREQS PEIVVDDKYQ ATFQEVWDSL NSESFENDFI 250 260 270 280 290 300 NQQYEDFKRT QKDGFPADMN QWEKDFAKYA STRAHFGDYQ FEDKQSNQFL DLPKDQDPYE 310 320 330 340 350 360 IGLQLMENGA KLSEAALAFE AAIQRDENHV DAWLKLGEVQ TQNEKEIAGI SALEKCLELH 370 380 390 400 410 420 PENSEALMNL AISYINEGYD NAAFATLERW ISTKYPQIVE KARQENPTIT DEDRFSLNKR 430 440 450 460 470 480 VTELFLNAAQ LSPNQASMDA DVQMGLGVLF YANEEFDKTI DCFKAALSIR PDDAILWNRL 490 500 510 520 530 540 GASLANSNRS EEAVDAYFKA LQLKPTFVRA RYNLGVSCIN IGCYKEAAEH LLSGLSMHQV 550 560 570 580 590 EGVDTVSTLN HNQSTSLTET LKRAFIAMER RDLLELVKPN MDLNQFRGEF SF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.
[2]	"Cloning and sequence of a 3.835 kbp DNA fragment containing the HIS4 gene and a fragment of a PEX5-like gene from Candida albicans." Navarro-Garcia F., Perez-Diaz R., Negredo A., Pla J., Nombela C. Yeast 14:1147-1157(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 297-592. Strain: ATCC 64385 / 1001.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACQ01000129 Genomic DNA. Translation: EAK94204.1. AACQ01000128 Genomic DNA. Translation: EAK94251.1. AJ003115 Genomic DNA. Translation: CAA05870.1.
RefSeq	XP_713321.1. XM_708228.1. XP_713368.1. XM_708275.1.
3D structure databases
ProteinModelPortal	O74711.
ModBase	Search...
Protein-protein interaction databases
STRING	5476.CAL0000635.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3644950. 3645026.
KEGG	cal:CaO19.13085. cal:CaO19.5640.
Organism-specific databases
CGD	CAL0000635. PEX5.
Phylogenomic databases
eggNOG	COG0457.
KO	K13342.
Family and domain databases
Gene3D	1.25.40.10. 2 hits.
InterPro	IPR024111. PTS1R_family. IPR001440. TPR-1. IPR013026. TPR-contain_dom. IPR011990. TPR-like_helical. IPR019734. TPR_repeat. [Graphical view]
PANTHER	PTHR10130. PTHR10130. 1 hit.
Pfam	PF00515. TPR_1. 2 hits. [Graphical view]
SMART	SM00028. TPR. 4 hits. [Graphical view]
PROSITE	PS50005. TPR. 4 hits. PS50293. TPR_REGION. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PEX5_CANAL

Accession

Primary (citable) accession number: O74711
Secondary accession number(s): Q59UT0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	January 24, 2006
Last modified:	April 3, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program