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O74702

- KEX1_PICPA

UniProt

O74702 - KEX1_PICPA

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Protein
Pheromone-processing carboxypeptidase KEX1
Gene
KEX1
Organism
Komagataella pastoris (Yeast) (Pichia pastoris)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Protease with a carboxypeptidase B-like function involved in the C-terminal processing of the lysine and arginine residues from protein precursors. Promotes cell fusion and is involved in the programmed cell death By similarity.1 Publication

Catalytic activityi

Preferential release of a C-terminal arginine or lysine residue.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei177 – 1771 By similarity
Active sitei383 – 3831 By similarity
Active sitei441 – 4411 By similarity

GO - Molecular functioni

  1. serine-type carboxypeptidase activity Source: InterPro

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Protease

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Pheromone-processing carboxypeptidase KEX1 (EC:3.4.16.6)
Alternative name(s):
Carboxypeptidase D
Gene namesi
Name:KEX1
OrganismiKomagataella pastoris (Yeast) (Pichia pastoris)
Taxonomic identifieri4922 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKomagataella

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini17 – 512496Lumenal Reviewed prediction
Add
BLAST
Transmembranei513 – 53321Helical; Reviewed prediction
Add
BLAST
Topological domaini534 – 62390Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616 Reviewed prediction
Add
BLAST
Chaini17 – 623607Pheromone-processing carboxypeptidase KEX1
PRO_0000411939Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi54 – 541N-linked (GlcNAc...) Reviewed prediction
Glycosylationi258 – 2581N-linked (GlcNAc...) Reviewed prediction
Glycosylationi430 – 4301N-linked (GlcNAc...) Reviewed prediction
Glycosylationi438 – 4381N-linked (GlcNAc...) Reviewed prediction
Glycosylationi469 – 4691N-linked (GlcNAc...) Reviewed prediction
Glycosylationi483 – 4831N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliO74702.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S10 family.

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR018202. Peptidase_S10_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O74702-1 [UniParc]FASTAAdd to Basket

« Hide

MLKLLCLLLP LVAVSASPID LGSQKDYLVL DLPGLSHLSE TQRPTMHAGL    50
LPLNLSFVAD DDTEYFFWRF SKQDVDRADI VFWLNGGPGC SSMDGALMEL 100
GPFVINPKQE VEYNEGTWVE AADVVFVDQP GGTGFSSTTN YLTELTEVAD 150
GFVTFLARYF HLFPADVYKK FTLGGESYAG QYVPYILKAI MDDLKSDSGQ 200
LPKELYLKGA LIGNGWIDPN EQSLSYLEFF IKKELIDIHG SYMPGLLQQQ 250
EKCQNLINHS SGEASESQIS YSACEKILND ALRFTRDKKA PLDQQCINMY 300
DYTLRDTYPS CGMSWPPYLP DVTAFLQKKS VLEALHLDSS ASWSECSARV 350
GSHLKNKISV PSVDILPDLL QEIPIILFNG DHDIICNCIG TERMIDKLEF 400
NGDQGFTEGT EYIPWFYNEV NVGKVISERN LTYVRVYNSS HMVPFDNTPV 450
SRGLLDIYFD NFEDVEYNNV SGIATPVYDV DKNITYIDSN DPRLQNGPKS 500
SSTDDSAAHG NPFFYYVFEL FVIVLLLCGL VYLYQYYSNS APHSILADKH 550
KKKSKNKSKN VRFLDDLESN LDLDNTDDKK DNSVMSKLLS SMGYQAQEPY 600
KPLDKGANAD LDIEMDSHGT SEK 623
Length:623
Mass (Da):70,018
Last modified:November 1, 1998 - v1
Checksum:iCC7B0683829D8FDB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF095574 Genomic DNA. Translation: AAC64191.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF095574 Genomic DNA. Translation: AAC64191.1 .

3D structure databases

ProteinModelPortali O74702.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.1820. 1 hit.
InterProi IPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR018202. Peptidase_S10_AS.
[Graphical view ]
PANTHERi PTHR11802. PTHR11802. 1 hit.
Pfami PF00450. Peptidase_S10. 1 hit.
[Graphical view ]
PRINTSi PR00724. CRBOXYPTASEC.
SUPFAMi SSF53474. SSF53474. 1 hit.
PROSITEi PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Disruption of the KEX1 gene in Pichia pastoris allows expression of full-length murine and human endostatin."
    Boehm T., Pirie-Shepherd S., Trinh L.B., Shiloach J., Folkman J.
    Yeast 15:563-572(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: SMD1168.

Entry informationi

Entry nameiKEX1_PICPA
AccessioniPrimary (citable) accession number: O74702
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: November 1, 1998
Last modified: June 11, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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