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O74702

- KEX1_PICPA

UniProt

O74702 - KEX1_PICPA

Protein

Pheromone-processing carboxypeptidase KEX1

Gene

KEX1

Organism
Komagataella pastoris (Yeast) (Pichia pastoris)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Protease with a carboxypeptidase B-like function involved in the C-terminal processing of the lysine and arginine residues from protein precursors. Promotes cell fusion and is involved in the programmed cell death By similarity.By similarity

    Catalytic activityi

    Preferential release of a C-terminal arginine or lysine residue.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei177 – 1771PROSITE-ProRule annotation
    Active sitei383 – 3831PROSITE-ProRule annotation
    Active sitei441 – 4411PROSITE-ProRule annotation

    GO - Molecular functioni

    1. serine-type carboxypeptidase activity Source: InterPro

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Carboxypeptidase, Hydrolase, Protease

    Keywords - Biological processi

    Apoptosis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pheromone-processing carboxypeptidase KEX1 (EC:3.4.16.6)
    Alternative name(s):
    Carboxypeptidase D
    Gene namesi
    Name:KEX1
    OrganismiKomagataella pastoris (Yeast) (Pichia pastoris)
    Taxonomic identifieri4922 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKomagataella

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi apparatus Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616Sequence AnalysisAdd
    BLAST
    Chaini17 – 623607Pheromone-processing carboxypeptidase KEX1PRO_0000411939Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi54 – 541N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi258 – 2581N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi430 – 4301N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi438 – 4381N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi469 – 4691N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi483 – 4831N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Structurei

    3D structure databases

    ProteinModelPortaliO74702.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini17 – 512496LumenalSequence AnalysisAdd
    BLAST
    Topological domaini534 – 62390CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei513 – 53321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase S10 family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR001563. Peptidase_S10.
    IPR018202. Peptidase_S10_AS.
    [Graphical view]
    PANTHERiPTHR11802. PTHR11802. 1 hit.
    PfamiPF00450. Peptidase_S10. 1 hit.
    [Graphical view]
    PRINTSiPR00724. CRBOXYPTASEC.
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS00131. CARBOXYPEPT_SER_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O74702-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLKLLCLLLP LVAVSASPID LGSQKDYLVL DLPGLSHLSE TQRPTMHAGL    50
    LPLNLSFVAD DDTEYFFWRF SKQDVDRADI VFWLNGGPGC SSMDGALMEL 100
    GPFVINPKQE VEYNEGTWVE AADVVFVDQP GGTGFSSTTN YLTELTEVAD 150
    GFVTFLARYF HLFPADVYKK FTLGGESYAG QYVPYILKAI MDDLKSDSGQ 200
    LPKELYLKGA LIGNGWIDPN EQSLSYLEFF IKKELIDIHG SYMPGLLQQQ 250
    EKCQNLINHS SGEASESQIS YSACEKILND ALRFTRDKKA PLDQQCINMY 300
    DYTLRDTYPS CGMSWPPYLP DVTAFLQKKS VLEALHLDSS ASWSECSARV 350
    GSHLKNKISV PSVDILPDLL QEIPIILFNG DHDIICNCIG TERMIDKLEF 400
    NGDQGFTEGT EYIPWFYNEV NVGKVISERN LTYVRVYNSS HMVPFDNTPV 450
    SRGLLDIYFD NFEDVEYNNV SGIATPVYDV DKNITYIDSN DPRLQNGPKS 500
    SSTDDSAAHG NPFFYYVFEL FVIVLLLCGL VYLYQYYSNS APHSILADKH 550
    KKKSKNKSKN VRFLDDLESN LDLDNTDDKK DNSVMSKLLS SMGYQAQEPY 600
    KPLDKGANAD LDIEMDSHGT SEK 623
    Length:623
    Mass (Da):70,018
    Last modified:November 1, 1998 - v1
    Checksum:iCC7B0683829D8FDB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF095574 Genomic DNA. Translation: AAC64191.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF095574 Genomic DNA. Translation: AAC64191.1 .

    3D structure databases

    ProteinModelPortali O74702.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR001563. Peptidase_S10.
    IPR018202. Peptidase_S10_AS.
    [Graphical view ]
    PANTHERi PTHR11802. PTHR11802. 1 hit.
    Pfami PF00450. Peptidase_S10. 1 hit.
    [Graphical view ]
    PRINTSi PR00724. CRBOXYPTASEC.
    SUPFAMi SSF53474. SSF53474. 1 hit.
    PROSITEi PS00131. CARBOXYPEPT_SER_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Disruption of the KEX1 gene in Pichia pastoris allows expression of full-length murine and human endostatin."
      Boehm T., Pirie-Shepherd S., Trinh L.B., Shiloach J., Folkman J.
      Yeast 15:563-572(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: SMD1168.

    Entry informationi

    Entry nameiKEX1_PICPA
    AccessioniPrimary (citable) accession number: O74702
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 27, 2011
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3