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Protein

Mitochondrial import inner membrane translocase subunit TIM9

Gene

TIM9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial intermembrane chaperone that participates in the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. Compared to TIM10, it may have a strong structural role.10 Publications

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein transporter activity Source: SGD
  • unfolded protein binding Source: SGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Protein transport, Translocation, Transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30387-MONOMER.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM9
Gene namesi
Name:TIM9
Ordered Locus Names:YEL020W-A
ORF Names:YEL020BW
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL020W-A.
SGDiS000007256. TIM9.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi40V → A in tim9-3; impairs the import of mitochondrial carrier proteins into mitochondria; when associated with P-60. 1 Publication1
Mutagenesisi52E → G in tim9-19; impairs the import of mitochondrial carrier proteins into mitochondria. 1 Publication1
Mutagenesisi60S → P in tim9-3; impairs the import of mitochondrial carrier proteins into mitochondria; when associated with A-40. 1 Publication1
Mutagenesisi67S → C: Impairs the import of mitochondrial carrier proteins into mitochondria. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001936101 – 87Mitochondrial import inner membrane translocase subunit TIM9Add BLAST87

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Disulfide bondi35 ↔ 591 Publication
Disulfide bondi39 ↔ 551 Publication

Keywords - PTMi

Acetylation, Disulfide bond

Proteomic databases

MaxQBiO74700.
PRIDEiO74700.

Interactioni

Subunit structurei

Heterohexamer; composed of 3 copies of TIM9 and 3 copies of TIM10, named soluble 70 kDa complex. Associates with the TIM12 component of the TIM22 complex, whose core is composed of TIM18, TIM22 and TIM54. Interacts with the transmembrane regions of multi-pass transmembrane proteins in transit.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TIM10P871087EBI-9108,EBI-9115

GO - Molecular functioni

  • unfolded protein binding Source: SGD

Protein-protein interaction databases

BioGridi36709. 15 interactors.
DIPiDIP-5806N.
IntActiO74700. 3 interactors.
MINTiMINT-4987906.

Structurei

Secondary structure

187
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi14 – 39Combined sources26
Beta strandi44 – 47Combined sources4
Helixi50 – 76Combined sources27

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DXRX-ray2.50A1-87[»]
ProteinModelPortaliO74700.
SMRiO74700.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO74700.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi35 – 59Twin CX3C motif1 PublicationAdd BLAST25

Domaini

The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIM9 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane (Probable).1 Publication

Sequence similaritiesi

Belongs to the small Tim family.Curated

Phylogenomic databases

GeneTreeiENSGT00450000040278.
HOGENOMiHOG000185786.
InParanoidiO74700.
KOiK17777.
OMAiSNLVERC.
OrthoDBiEOG092C5RBL.

Family and domain databases

Gene3Di1.10.287.810. 1 hit.
InterProiIPR004217. Tim10/DDP_fam_Znf.
[Graphical view]
PfamiPF02953. zf-Tim10_DDP. 1 hit.
[Graphical view]
SUPFAMiSSF144122. SSF144122. 1 hit.

Sequencei

Sequence statusi: Complete.

O74700-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDALNSKEQQ EFQKVVEQKQ MKDFMRLYSN LVERCFTDCV NDFTTSKLTN
60 70 80
KEQTCIMKCS EKFLKHSERV GQRFQEQNAA LGQGLGR
Length:87
Mass (Da):10,202
Last modified:November 1, 1998 - v1
Checksum:i5160E5DBA52065C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18530 Genomic DNA. No translation available.
AF093244 Genomic DNA. Translation: AAC83169.1.
AY557798 Genomic DNA. Translation: AAS56124.1.
BK006939 Genomic DNA. Translation: DAA07632.1.
PIRiS78717.
RefSeqiNP_010894.1. NM_001184349.1.

Genome annotation databases

EnsemblFungiiYEL020W-A; YEL020W-A; YEL020W-A.
GeneIDi856693.
KEGGisce:YEL020W-A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18530 Genomic DNA. No translation available.
AF093244 Genomic DNA. Translation: AAC83169.1.
AY557798 Genomic DNA. Translation: AAS56124.1.
BK006939 Genomic DNA. Translation: DAA07632.1.
PIRiS78717.
RefSeqiNP_010894.1. NM_001184349.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DXRX-ray2.50A1-87[»]
ProteinModelPortaliO74700.
SMRiO74700.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36709. 15 interactors.
DIPiDIP-5806N.
IntActiO74700. 3 interactors.
MINTiMINT-4987906.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Proteomic databases

MaxQBiO74700.
PRIDEiO74700.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYEL020W-A; YEL020W-A; YEL020W-A.
GeneIDi856693.
KEGGisce:YEL020W-A.

Organism-specific databases

EuPathDBiFungiDB:YEL020W-A.
SGDiS000007256. TIM9.

Phylogenomic databases

GeneTreeiENSGT00450000040278.
HOGENOMiHOG000185786.
InParanoidiO74700.
KOiK17777.
OMAiSNLVERC.
OrthoDBiEOG092C5RBL.

Enzyme and pathway databases

BioCyciYEAST:G3O-30387-MONOMER.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Miscellaneous databases

EvolutionaryTraceiO74700.
PROiO74700.

Family and domain databases

Gene3Di1.10.287.810. 1 hit.
InterProiIPR004217. Tim10/DDP_fam_Znf.
[Graphical view]
PfamiPF02953. zf-Tim10_DDP. 1 hit.
[Graphical view]
SUPFAMiSSF144122. SSF144122. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTIM9_YEAST
AccessioniPrimary (citable) accession number: O74700
Secondary accession number(s): D3DLM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.