Reviewed, UniProtKB/Swiss-Prot O74634 (SYMM_CANAL)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase, mitochondrial
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:

MSM1

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
Subcellular location	Mitochondrion matrix.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 577	577	Methionyl-tRNA synthetase, mitochondrial		PRO_0000139270
Regions
	Motif	25 – 37	13	"HIGH" region
	Motif	329 – 333	5	"KMSKS" region
Sites
	Binding site	332	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O74634-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 129413ACD5B14728	FASTA	577	67,120
	10 20 30 40 50 60 MRFKIRGPLI QLRYKSTKAF YITTPIFYVN AAPHIGHLYS MLIADTRNKW EKLNPSKESF 70 80 90 100 110 120 MLTGTDEHGL KIQSTAEKLG LEPKVLVDKV SQNFSKLAEQ FDVNYDRFIR TTDNDHIELV 130 140 150 160 170 180 RYFWNLMMEK GFIYTDTHSG WYSISDETFF PETQIEEVVK NGKAVKISSE TKNEVVYQEE 190 200 210 220 230 240 TNYFFKLSMF QEQLIQFLKQ NPEFIKPKHR YQFILKELED TKLPDLSISR PSSRLKWSIE 250 260 270 280 290 300 VPNDSTQKIY VWFDALLNYL TATKFPHGFE VQDSKFVTPE NSIWPATHVI GKDIIRFHCI 310 320 330 340 350 360 YWPIFLMAAG IELPKQVIVH SHWLCDGFKM SKSLGNLVDP MEISEYYGVD PVRFFLVENS 370 380 390 400 410 420 NIDDDCKFSE ELLQRSRDAV LGKYCNLISR IGGKNFSIEE AVKSFASGEF NNIREIIETY 430 440 450 460 470 480 TINKDSVEGL LSSLNKLTTD LNDLYNQMDH YFTNFDYIRA IQCWWSVINQ ANQIFQSAEP 490 500 510 520 530 540 WTYVKLINSP ETPAELKEKY RILNNYFVYL CAETTRISSI LIQPVMPQLS KKILDRLNVS 550 560 570 GRTSEFTTLS ADLQYGSGAN SKSHKVPLEK IAPRDIK

References

[1]	"Cloning and characterization of mitochondrial methionyl-tRNA synthetase from a pathogenic fungi Candida albicans." Lee S.W., Jo Y.J., Kim S. Gene 215:311-318(1998) [PubMed: 9714830] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB006140 Genomic DNA. Translation: BAA33373.1.
3D structure databases
HSSP	HSSP built from PDB template 1A8H based on UniProtKB P23395.
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.1.1.10. 1124.
Family and domain databases
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYMM_CANAL

Accession

Primary (citable) accession number: O74634

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1998
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)