Reviewed, UniProtKB/Swiss-Prot O74633 (RPA2_NEUCR)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase I subunit RPA2
    EC=2.7.7.6
Alternative name(s):
    DNA-directed RNA polymerase I polypeptide 2
      Short name=RNA polymerase I subunit 2

Gene names

Name:	acr-2
ORF Names:	NCU08616

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	1234 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Component of the RNA polymerase I (Pol I) complex consisting of 14 subunits By similarity.
Subcellular location	Nucleus › nucleolus By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1234	1234	DNA-directed RNA polymerase I subunit RPA2		PRO_0000048078
Regions
	Zinc finger	1119 – 1150	32	C4-type Potential
Experimental info
	Mutagenesis	976	1	G → D in un-18; shows a TS phenotype with respect to both mycelial growth and the period length of the conidiation rhythm.
	Sequence conflict	294 – 299	6	GVANTF → ASPTPS in BAA33445. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O74633-1 [UniParc]. Last modified August 31, 2004. Version 2. Checksum: 8840AA85D52BBAE0	FASTA	1,234	138,601
	10 20 30 40 50 60 MAPQQPQPTS QDWDVEFNQV RREKLFRDPP TDRTAYPALQ AAVDPHIESF NALFRDDGKP 70 80 90 100 110 120 SLLDHALAEI GTKTFLDGDE RADPQGKNKL TIRYKSIELQ KSQVPPTNRW AKNREIFPAE 130 140 150 160 170 180 CRERHVSYRG KLSATFEYRI NDGEPHEFVR ELGQMPIMVK SNKCHLQNNS PAQLVARKEE 190 200 210 220 230 240 SEELGGYFIV NGIEKLIRML LVNRRNFPLA IVRPSFQNRG ASYTPYGIIM RSVRPDETSQ 250 260 270 280 290 300 TNVLHYLSDG NVTFRFSWRK NEYLIPVMMI MKALVETNDR EIFEGLVGPP QSKGVANTFL 310 320 330 340 350 360 TDRVELLLRT YKKYGLYSKT QTRAYLGQKF RVVLGVPDTM SDYEVGTEFL RKIVLVHLGS 370 380 390 400 410 420 QDVTEQQDAD KFNMLLFMCR KLYALVAGDC AVDNPDAVQN QEILLGGFLY GQIIKERLEE 430 440 450 460 470 480 LLTVSFRASL RDYLRRNPTV SFQSDTFLKD FPIAIFRRAN ENIGQSLEYF LSTGNLVSPS 490 500 510 520 530 540 GLDLQQVSGF TVVAEKLNFL RFISHFRMVH RGSFFAQLKT TTVRKLLPES WGFLCPVHTP 550 560 570 580 590 600 DGSPCGLLNH LAHKCKIMTE SVDASTISRL AFELGVVNIS SAATSESVVV MLDGRIVGWC 610 620 630 640 650 660 TPEECKSIAE TLRYWKVNGE NGVPLQLEIG YVPPSNGGSY PGLYMSSQPA RMVRPVKYLP 670 680 690 700 710 720 LQKEDFVGPQ EQPYMSIACT EQEVIPGDST HVEFDPTNIL SILANMTPFS DFNQSPRNMY 730 740 750 760 770 780 QCQMGKQTMG TPATALAHRT DNKMYRLQTG QTPVVRAPLH NTYGFDNFPN GMNAVVAVIS 790 800 810 820 830 840 YTGYDMDDAM ILNKSAHERG FGHGSIYKTK KVSLKDDSRT RSAKSIVKMF GFAPNSTIRE 850 860 870 880 890 900 STRDMLDNDG LPRVGRLLRE GDVICAWHTV SADYNGQLVN RDGVTHYERY KDSEDAFVEE 910 920 930 940 950 960 VRVIGADNGT EPLQTVSIKL RIPRSPVIGD KFSSRHGQKG VLSQKWPATD MPFSETGIQP 970 980 990 1000 1010 1020 DVIINPHAFP SRMTIGMFVE SLAGKAGALH GLAQDSTPFK FDEQNTAGDY FGHQLMKAGY 1030 1040 1050 1060 1070 1080 NYHGNEPLYS GITGEEFQAD IYIGVVYYQR LRHMVNDKYQ VRTTGPVVPT TGQPIKGRKK 1090 1100 1110 1120 1130 1140 GGGIRVGEME RDALLAHGTS FLLQDRLLNC SDYSKSWMCR QCGSFLSTQP TVSPFIGKRK 1150 1160 1170 1180 1190 1200 AVSTVRCRNC AVRLDDMEDV DLMQIDGEIW EDGSGTQWIG GENTTIVAVP GALKYLDVEL 1210 1220 1230 AAMGIKLKYK VDKKDEIRRG QLVGKKAGDL MLTA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mutation of the gene for the second-largest subunit of RNA polymerase I prolongs the period length of the circadian conidiation rhythm in Neurospora crassa." Onai K., Katagiri S., Akiyama M., Nakashima H. Mol. Gen. Genet. 259:264-271(1998) [PubMed: 9749669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: UN-18.
[2]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB006052 Genomic DNA. Translation: BAA33445.1. AABX02000058 Genomic DNA. Translation: EAA35588.1.
PIR	T30515.
RefSeq	XP_964824.1.
3D structure databases
HSSP	HSSP built from PDB template 1I50 based on UniProtKB P08518.
ModBase	Search...
Protein-protein interaction databases
STRING	O74633.
Genome annotation databases
GeneID	3880984.
KEGG	ncr:NCU08616.
NMPDR	fig|5141.1.peg.7673.
UCSC	K11G12.2. c. elegans.
Enzyme and pathway databases
BRENDA	2.7.7.6. 266.
Family and domain databases
InterPro	IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR009674. RNA_pol_Rpa2-specific. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007647. RNA_pol_Rpb2_5. IPR007641. RNA_pol_Rpb2_7. [Graphical view]
PANTHER	PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF06883. RNA_pol_Rpa2_4. 1 hit. PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 1 hit. PF04565. RNA_pol_Rpb2_3. 1 hit. PF04567. RNA_pol_Rpb2_5. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPA2_NEUCR

Accession

Primary (citable) accession number: O74633
Secondary accession number(s): Q7RVL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	August 31, 2004
Last modified:	September 1, 2009
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)