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Protein

NEDD8-conjugating enzyme ubc12

Gene

ubc12

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Accepts the ubiquitin-like protein NEDD8/RUB1 from the UBA3-ULA1 E1 complex and catalyzes its covalent attachment to other proteins.By similarity

Catalytic activityi

ATP + NEDD8 + protein lysine = AMP + diphosphate + protein N-NEDD8yllysine.

Pathwayi: protein neddylation

This protein is involved in the pathway protein neddylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein neddylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei105Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-8951664 Neddylation
R-SPO-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00885

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8-conjugating enzyme ubc12 (EC:2.3.2.-)
Alternative name(s):
RUB1-conjugating enzyme
Ubiquitin carrier protein 12
Gene namesi
Name:ubc12
ORF Names:SPCC777.10c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC777.10c
PomBaseiSPCC777.10c ubc12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000824991 – 177NEDD8-conjugating enzyme ubc12Add BLAST177

Proteomic databases

MaxQBiO74549
PaxDbiO74549
PRIDEiO74549

Interactioni

Protein-protein interaction databases

BioGridi275981, 1 interactor
STRINGi4896.SPCC777.10c.1

Structurei

3D structure databases

ProteinModelPortaliO74549
SMRiO74549
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000233456
InParanoidiO74549
KOiK10579
OMAiMIKIWSM
OrthoDBiEOG092C5L1U
PhylomeDBiO74549

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

O74549-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKIWELKKK EAQKEKNASG ISPAQIRIQK DVTDLEIPST MSTSWPDPIK
60 70 80 90 100
LNVLHLEIRP DEGYYKGGKF KFRIQIDDNY PHDPPKVKCL NKIYHPNIDI
110 120 130 140 150
EGNVCLNILR QDWNPVLNLN SILVGLQFLF LSPNAEDPLN KEAAADLHKD
160 170
PQGFASRVRT AMKGGLVNGI SFDNVMA
Length:177
Mass (Da):20,062
Last modified:November 1, 1998 - v1
Checksum:iEDCF481E7A6759E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA Translation: CAA20714.1
PIRiT11716
RefSeqiNP_588256.1, NM_001023246.2

Genome annotation databases

EnsemblFungiiSPCC777.10c.1; SPCC777.10c.1:pep; SPCC777.10c
GeneIDi2539416
KEGGispo:SPCC777.10c

Similar proteinsi

Entry informationi

Entry nameiUBC12_SCHPO
AccessioniPrimary (citable) accession number: O74549
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: November 1, 1998
Last modified: April 25, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health