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Protein

Peroxide stress-activated histidine kinase mak3

Gene

mak3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the control of the SAPK-dependent transcriptional response to peroxide stress. Regulates sty1 activity.2 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

GO - Biological processi

  • negative regulation of G2/M transition of mitotic cell cycle Source: PomBase
  • negative regulation of induction of conjugation with cellular fusion Source: PomBase
  • phosphorelay signal transduction system Source: PomBase
  • protein phosphorylation Source: PomBase
  • stress-activated protein kinase signaling cascade Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxide stress-activated histidine kinase mak3 (EC:2.7.13.3)
Alternative name(s):
His-Asp phosphorelay kinase phk2
Mcs4-associated kinase 3
Gene namesi
Name:mak3
Synonyms:phk2
ORF Names:SPCC74.06
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC74.06.
PomBaseiSPCC74.06. mak3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000814101 – 2344Peroxide stress-activated histidine kinase mak3Add BLAST2344

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12Phosphoserine1 Publication1
Modified residuei16Phosphoserine1 Publication1
Modified residuei17Phosphoserine1 Publication1
Modified residuei1795Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1
Modified residuei22634-aspartylphosphatePROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO74539.
PRIDEiO74539.

PTM databases

iPTMnetiO74539.

Interactioni

Protein-protein interaction databases

BioGridi275689. 47 interactors.
MINTiMINT-4679890.

Structurei

3D structure databases

ProteinModelPortaliO74539.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 295Protein kinasePROSITE-ProRule annotationAdd BLAST295
Repeati829 – 862TPR 1Add BLAST34
Repeati1340 – 1373TPR 2Add BLAST34
Domaini1730 – 1781PACPROSITE-ProRule annotationAdd BLAST52
Domaini1792 – 2018Histidine kinasePROSITE-ProRule annotationAdd BLAST227
Domaini2211 – 2333Response regulatoryPROSITE-ProRule annotationAdd BLAST123

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi983 – 986Poly-Asp4
Compositional biasi2099 – 2104Poly-Val6

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 PAC (PAS-associated C-terminal) domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation
Contains 2 TPR repeats.Curated

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

InParanoidiO74539.
OMAiEACTGAY.
OrthoDBiEOG092C01WK.
PhylomeDBiO74539.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
1.25.40.10. 2 hits.
3.30.450.40. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR003018. GAF.
IPR029016. GAF_dom-like.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR011009. Kinase-like_dom.
IPR027417. P-loop_NTPase.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013655. PAS_fold_3.
IPR000719. Prot_kinase_dom.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF13185. GAF_2. 1 hit.
PF00512. HisKA. 1 hit.
PF08447. PAS_3. 1 hit.
PF00069. Pkinase. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
SMARTiSM00388. HisKA. 1 hit.
SM00086. PAC. 1 hit.
SM00448. REC. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF52540. SSF52540. 3 hits.
SSF55781. SSF55781. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50113. PAC. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O74539-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYSQHELRNK VSLALSSLLR YTFELTPFFE LYEADFAYAL YAGFELATNR
60 70 80 90 100
KVVGKFSFQN VHLENEYNIL TEIAKDERAS KFSPTPIEFT SFPHIDLSAC
110 120 130 140 150
IAYDFGHGAE LSTSYAYFRE NPAEFVRFCI AICKCIEYLH SKGMVHGEIR
160 170 180 190 200
LDSFIPISSY DNVYMLTVGS GASYFHNCLQ AHNWRKYSED SESMSRILFI
210 220 230 240 250
SPEQTGRTSY SVGYRTDIYS LGVLFFHYLS DCSPYTGSFV QRIRSILTEP
260 270 280 290 300
LPDISKSCPK LPHLIFKIIE KMTRKNPDER YTSCSGIVND LEACLDDIDK
310 320 330 340 350
GLILNDHVLE KTGRTSLFYL PCSIYGREHE IKLIRKILRN SPRAINHQDK
360 370 380 390 400
KDLETFNPYY LNAIESESSS QSLSLSQRAS EVMPLVILIT GCEGIGESSL
410 420 430 440 450
IQTICDRREG YMAITKFEVS QSIVYSAIVS AVAEFIRQIL AEDQLLLNNF
460 470 480 490 500
FEELKNKLES DLYLLDSVFD LVPEIRSLLQ QFSTSSGNTR KTSLLGSNHS
510 520 530 540 550
SYSDKLGSPT ILSTSFSLAR PYPEPALVSP STERPPRSSF SAALMTLLNI
560 570 580 590 600
IASFKKVTMV IENIHLADES SLIILQKIVY SDLPLTLMIT CDKENDHVIN
610 620 630 640 650
RFRLANDRIH EIELKPLSFN AVNSYVQATL HRTDDGLARF SSYVYHISKG
660 670 680 690 700
VPLLVRNVLL SIYENKIIYF DWKKNRWEVN YDEMYTLDND YSEPDAFMTA
710 720 730 740 750
KKKISKLNDS SRAILGWASL LGPSFSFATV KKLCKDTDNI ELNVEALQSA
760 770 780 790 800
LREGIIYATS SDDTYTFSRS IYVKAMRDLL NEAKIQIMHA CLIDVCLKNR
810 820 830 840 850
DRYNIFDIAF HINAAFDFVK GDKRSVEYCH YLHLAAEEAL KIGANQEALD
860 870 880 890 900
LYNRCIKMIP HEIPEESDDS YIRCQLIGMY VGCAEAYWVN DNFDTASEML
910 920 930 940 950
KLAEEKACNN SEVFPARFLY SRILFEGVHI EECTQYVLSC LKPLGYELKR
960 970 980 990 1000
HSLEDSKSII SALIPRIIDK ITKSSEESQS STDDDDRRIF EILSFLYVGS
1010 1020 1030 1040 1050
VATSYFSETA EMAIDFGIAQ VEFFLSTVVN SFSAFALVYF AILANSLLEP
1060 1070 1080 1090 1100
SEDILFIGNY GEKLNREAEN PIIFSRTEYL YVQSLGFIDS TTKERRLTID
1110 1120 1130 1140 1150
YLDRNCVTCS DKHVIISLLL VSSWEKFLTS NNYSNYLADF ETTHAQIMEM
1160 1170 1180 1190 1200
KPWVGDTSLI TQLKRFLMCL QDNIKLDLIK SKSFLSDHNI QLSSPAAQES
1210 1220 1230 1240 1250
AKLAFSLHGW INSWYLLALV MHGEWDMAIS YGENFKREFK NALLTSSRVF
1260 1270 1280 1290 1300
GIFMFTWSLV NKMLICPEFT KQKKYYEQYK ENLGFFDSLC IGDNECITRV
1310 1320 1330 1340 1350
YFLLLKACGL IMNGLNFEAS VMLEEVISLT EKLELFLLQA FAFETVGSIF
1360 1370 1380 1390 1400
VSMELYTSAT QYLEEAIRNY AALGVKQKAR HLRDKFGDLL VSNNLQVSID
1410 1420 1430 1440 1450
EATQTDFPLV FSPERSSIDI NASSMRSEKA SFEIPFPEEQ IDDDVSPVAQ
1460 1470 1480 1490 1500
DSSLEELLIS LDIIDLTSVM RSCQTIASEI ELTGLLSTMT QRMLEDSSAN
1510 1520 1530 1540 1550
AAVIAIRDDV GFKIAAYRTG ELNEVFAPPM PITEDQTYVP SRVINYVVHT
1560 1570 1580 1590 1600
QKALFSNNIN HEFDLQQERW NIENHMGRSV IAIPLYQKKE VFAILYLQGP
1610 1620 1630 1640 1650
PSAFHSRHMS VLSILGAQAS FAIVNISLFH KVKEATNVNT IIIKAQREAL
1660 1670 1680 1690 1700
NLVQKSEAKY RSFVDTMPCL LSKLEFDEEL RIELFGSFWK EYCGELNIND
1710 1720 1730 1740 1750
PNTWKEYVHL DDHLKLQDFL LSHLHNPLPF ELEIRIKRKD GVYRWNLTRC
1760 1770 1780 1790 1800
TPTTNEKNRT SFLCATIDID DQKKARATAL ELARLRSNFL ANISHELRTP
1810 1820 1830 1840 1850
FSGFYGMLSL LDDTNLDSEQ RDIVSAARIS CEMLLRVIND LLNFSKLEAG
1860 1870 1880 1890 1900
KVTLESDLEF SLESVVCDCM QSVYSACAEK GINLSYNVSP DIPFFTAGDG
1910 1920 1930 1940 1950
MKIGQMLKSI LDNSVKTVNN GFIRVRAFLA GSSKKNDRDQ LQIAFIVEDT
1960 1970 1980 1990 2000
REESNAIFLA NMINSLNRGC NDYLPMDLSG TALGMSTCLQ LCKIMGGSVS
2010 2020 2030 2040 2050
VEVSQNNPTF KICYDLKIHE LGKERYDIIA TPLFQNLTEF NDLIKSKVAI
2060 2070 2080 2090 2100
RVSKTSTEYD NITTYLQAAR KVLHVFKGLQ DLASIFDLSP DSALLRCSVV
2110 2120 2130 2140 2150
VVDVYSMDDV KAVEKILKSY PDVHVIYLCC DPSRLNIEQE LQKPSGRSFA
2160 2170 2180 2190 2200
CKKRWGFLQM PCTRENFLKV TLQVFKSNED TCNFYSYVNE YGESPKPDDD
2210 2220 2230 2240 2250
MDRLNKCVGS KILIAEDNPI VRMTLKKQLE HLGMDVDAAE DGKETLQIFE
2260 2270 2280 2290 2300
SHPDNYYQVC FVDYHMPVYD GLEVTRRMRK IERKHGCAPL PIFALTADMQ
2310 2320 2330 2340
PTMETQFQEV GITHYLSKPF KKETLIKMLL QYLVNGTDGN ANTS
Length:2,344
Mass (Da):266,850
Last modified:November 1, 1998 - v1
Checksum:i8032B9DBD3E1BF5C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA20836.1.
PIRiT41590.
RefSeqiNP_588379.1. NM_001023370.2.

Genome annotation databases

EnsemblFungiiSPCC74.06.1; SPCC74.06.1:pep; SPCC74.06.
GeneIDi2539117.
KEGGispo:SPCC74.06.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA20836.1.
PIRiT41590.
RefSeqiNP_588379.1. NM_001023370.2.

3D structure databases

ProteinModelPortaliO74539.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275689. 47 interactors.
MINTiMINT-4679890.

PTM databases

iPTMnetiO74539.

Proteomic databases

MaxQBiO74539.
PRIDEiO74539.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC74.06.1; SPCC74.06.1:pep; SPCC74.06.
GeneIDi2539117.
KEGGispo:SPCC74.06.

Organism-specific databases

EuPathDBiFungiDB:SPCC74.06.
PomBaseiSPCC74.06. mak3.

Phylogenomic databases

InParanoidiO74539.
OMAiEACTGAY.
OrthoDBiEOG092C01WK.
PhylomeDBiO74539.

Miscellaneous databases

PROiO74539.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
1.25.40.10. 2 hits.
3.30.450.40. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR003018. GAF.
IPR029016. GAF_dom-like.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR011009. Kinase-like_dom.
IPR027417. P-loop_NTPase.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013655. PAS_fold_3.
IPR000719. Prot_kinase_dom.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF13185. GAF_2. 1 hit.
PF00512. HisKA. 1 hit.
PF08447. PAS_3. 1 hit.
PF00069. Pkinase. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
SMARTiSM00388. HisKA. 1 hit.
SM00086. PAC. 1 hit.
SM00448. REC. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF52540. SSF52540. 3 hits.
SSF55781. SSF55781. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50113. PAC. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAK3_SCHPO
AccessioniPrimary (citable) accession number: O74539
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.