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Protein

Peroxide stress-activated histidine kinase mak3

Gene

mak3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the control of the SAPK-dependent transcriptional response to peroxide stress. Regulates sty1 activity.2 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

GO - Biological processi

  • cellular response to oxidative stress Source: PomBase
  • detection of oxidative stress Source: PomBase
  • phosphorelay signal transduction system Source: PomBase
  • positive regulation of stress-activated MAPK cascade Source: PomBase

Keywordsi

Molecular functionKinase, Transferase
Biological processTwo-component regulatory system
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxide stress-activated histidine kinase mak3 (EC:2.7.13.3)
Alternative name(s):
His-Asp phosphorelay kinase phk2
Mcs4-associated kinase 3
Gene namesi
Name:mak3
Synonyms:phk2
ORF Names:SPCC74.06
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC74.06
PomBaseiSPCC74.06 mak3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000814101 – 2344Peroxide stress-activated histidine kinase mak3Add BLAST2344

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12Phosphoserine1 Publication1
Modified residuei16Phosphoserine1 Publication1
Modified residuei17Phosphoserine1 Publication1
Modified residuei1795Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1
Modified residuei22634-aspartylphosphatePROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO74539
PaxDbiO74539
PRIDEiO74539

PTM databases

iPTMnetiO74539

Interactioni

Protein-protein interaction databases

BioGridi27568947 interactors.
STRINGi4896.SPCC74.06.1

Structurei

3D structure databases

ProteinModelPortaliO74539
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 295Protein kinasePROSITE-ProRule annotationAdd BLAST295
Repeati829 – 862TPR 1Add BLAST34
Repeati1340 – 1373TPR 2Add BLAST34
Domaini1730 – 1781PACPROSITE-ProRule annotationAdd BLAST52
Domaini1792 – 2018Histidine kinasePROSITE-ProRule annotationAdd BLAST227
Domaini2211 – 2333Response regulatoryPROSITE-ProRule annotationAdd BLAST123

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi983 – 986Poly-Asp4
Compositional biasi2099 – 2104Poly-Val6

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

InParanoidiO74539
OMAiYLENRLM
OrthoDBiEOG092C01WK
PhylomeDBiO74539

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
cd00082 HisKA, 1 hit
cd00130 PAS, 1 hit
cd00156 REC, 1 hit
Gene3Di3.30.450.401 hit
3.30.565.101 hit
InterProiView protein in InterPro
IPR011006 CheY-like_superfamily
IPR003018 GAF
IPR029016 GAF-like_dom_sf
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR005467 His_kinase_dom
IPR003661 HisK_dim/P
IPR036097 HisK_dim/P_sf
IPR011009 Kinase-like_dom_sf
IPR027417 P-loop_NTPase
IPR001610 PAC
IPR000014 PAS
IPR000700 PAS-assoc_C
IPR035965 PAS-like_dom_sf
IPR013655 PAS_fold_3
IPR000719 Prot_kinase_dom
IPR001789 Sig_transdc_resp-reg_receiver
PfamiView protein in Pfam
PF13185 GAF_2, 1 hit
PF00512 HisKA, 1 hit
PF08447 PAS_3, 1 hit
PF00069 Pkinase, 1 hit
PF00072 Response_reg, 1 hit
SMARTiView protein in SMART
SM00388 HisKA, 1 hit
SM00086 PAC, 1 hit
SM00448 REC, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF47384 SSF47384, 1 hit
SSF52172 SSF52172, 1 hit
SSF52540 SSF52540, 3 hits
SSF55785 SSF55785, 1 hit
SSF55874 SSF55874, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50109 HIS_KIN, 1 hit
PS50113 PAC, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS50110 RESPONSE_REGULATORY, 1 hit

Sequencei

Sequence statusi: Complete.

O74539-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYSQHELRNK VSLALSSLLR YTFELTPFFE LYEADFAYAL YAGFELATNR
60 70 80 90 100
KVVGKFSFQN VHLENEYNIL TEIAKDERAS KFSPTPIEFT SFPHIDLSAC
110 120 130 140 150
IAYDFGHGAE LSTSYAYFRE NPAEFVRFCI AICKCIEYLH SKGMVHGEIR
160 170 180 190 200
LDSFIPISSY DNVYMLTVGS GASYFHNCLQ AHNWRKYSED SESMSRILFI
210 220 230 240 250
SPEQTGRTSY SVGYRTDIYS LGVLFFHYLS DCSPYTGSFV QRIRSILTEP
260 270 280 290 300
LPDISKSCPK LPHLIFKIIE KMTRKNPDER YTSCSGIVND LEACLDDIDK
310 320 330 340 350
GLILNDHVLE KTGRTSLFYL PCSIYGREHE IKLIRKILRN SPRAINHQDK
360 370 380 390 400
KDLETFNPYY LNAIESESSS QSLSLSQRAS EVMPLVILIT GCEGIGESSL
410 420 430 440 450
IQTICDRREG YMAITKFEVS QSIVYSAIVS AVAEFIRQIL AEDQLLLNNF
460 470 480 490 500
FEELKNKLES DLYLLDSVFD LVPEIRSLLQ QFSTSSGNTR KTSLLGSNHS
510 520 530 540 550
SYSDKLGSPT ILSTSFSLAR PYPEPALVSP STERPPRSSF SAALMTLLNI
560 570 580 590 600
IASFKKVTMV IENIHLADES SLIILQKIVY SDLPLTLMIT CDKENDHVIN
610 620 630 640 650
RFRLANDRIH EIELKPLSFN AVNSYVQATL HRTDDGLARF SSYVYHISKG
660 670 680 690 700
VPLLVRNVLL SIYENKIIYF DWKKNRWEVN YDEMYTLDND YSEPDAFMTA
710 720 730 740 750
KKKISKLNDS SRAILGWASL LGPSFSFATV KKLCKDTDNI ELNVEALQSA
760 770 780 790 800
LREGIIYATS SDDTYTFSRS IYVKAMRDLL NEAKIQIMHA CLIDVCLKNR
810 820 830 840 850
DRYNIFDIAF HINAAFDFVK GDKRSVEYCH YLHLAAEEAL KIGANQEALD
860 870 880 890 900
LYNRCIKMIP HEIPEESDDS YIRCQLIGMY VGCAEAYWVN DNFDTASEML
910 920 930 940 950
KLAEEKACNN SEVFPARFLY SRILFEGVHI EECTQYVLSC LKPLGYELKR
960 970 980 990 1000
HSLEDSKSII SALIPRIIDK ITKSSEESQS STDDDDRRIF EILSFLYVGS
1010 1020 1030 1040 1050
VATSYFSETA EMAIDFGIAQ VEFFLSTVVN SFSAFALVYF AILANSLLEP
1060 1070 1080 1090 1100
SEDILFIGNY GEKLNREAEN PIIFSRTEYL YVQSLGFIDS TTKERRLTID
1110 1120 1130 1140 1150
YLDRNCVTCS DKHVIISLLL VSSWEKFLTS NNYSNYLADF ETTHAQIMEM
1160 1170 1180 1190 1200
KPWVGDTSLI TQLKRFLMCL QDNIKLDLIK SKSFLSDHNI QLSSPAAQES
1210 1220 1230 1240 1250
AKLAFSLHGW INSWYLLALV MHGEWDMAIS YGENFKREFK NALLTSSRVF
1260 1270 1280 1290 1300
GIFMFTWSLV NKMLICPEFT KQKKYYEQYK ENLGFFDSLC IGDNECITRV
1310 1320 1330 1340 1350
YFLLLKACGL IMNGLNFEAS VMLEEVISLT EKLELFLLQA FAFETVGSIF
1360 1370 1380 1390 1400
VSMELYTSAT QYLEEAIRNY AALGVKQKAR HLRDKFGDLL VSNNLQVSID
1410 1420 1430 1440 1450
EATQTDFPLV FSPERSSIDI NASSMRSEKA SFEIPFPEEQ IDDDVSPVAQ
1460 1470 1480 1490 1500
DSSLEELLIS LDIIDLTSVM RSCQTIASEI ELTGLLSTMT QRMLEDSSAN
1510 1520 1530 1540 1550
AAVIAIRDDV GFKIAAYRTG ELNEVFAPPM PITEDQTYVP SRVINYVVHT
1560 1570 1580 1590 1600
QKALFSNNIN HEFDLQQERW NIENHMGRSV IAIPLYQKKE VFAILYLQGP
1610 1620 1630 1640 1650
PSAFHSRHMS VLSILGAQAS FAIVNISLFH KVKEATNVNT IIIKAQREAL
1660 1670 1680 1690 1700
NLVQKSEAKY RSFVDTMPCL LSKLEFDEEL RIELFGSFWK EYCGELNIND
1710 1720 1730 1740 1750
PNTWKEYVHL DDHLKLQDFL LSHLHNPLPF ELEIRIKRKD GVYRWNLTRC
1760 1770 1780 1790 1800
TPTTNEKNRT SFLCATIDID DQKKARATAL ELARLRSNFL ANISHELRTP
1810 1820 1830 1840 1850
FSGFYGMLSL LDDTNLDSEQ RDIVSAARIS CEMLLRVIND LLNFSKLEAG
1860 1870 1880 1890 1900
KVTLESDLEF SLESVVCDCM QSVYSACAEK GINLSYNVSP DIPFFTAGDG
1910 1920 1930 1940 1950
MKIGQMLKSI LDNSVKTVNN GFIRVRAFLA GSSKKNDRDQ LQIAFIVEDT
1960 1970 1980 1990 2000
REESNAIFLA NMINSLNRGC NDYLPMDLSG TALGMSTCLQ LCKIMGGSVS
2010 2020 2030 2040 2050
VEVSQNNPTF KICYDLKIHE LGKERYDIIA TPLFQNLTEF NDLIKSKVAI
2060 2070 2080 2090 2100
RVSKTSTEYD NITTYLQAAR KVLHVFKGLQ DLASIFDLSP DSALLRCSVV
2110 2120 2130 2140 2150
VVDVYSMDDV KAVEKILKSY PDVHVIYLCC DPSRLNIEQE LQKPSGRSFA
2160 2170 2180 2190 2200
CKKRWGFLQM PCTRENFLKV TLQVFKSNED TCNFYSYVNE YGESPKPDDD
2210 2220 2230 2240 2250
MDRLNKCVGS KILIAEDNPI VRMTLKKQLE HLGMDVDAAE DGKETLQIFE
2260 2270 2280 2290 2300
SHPDNYYQVC FVDYHMPVYD GLEVTRRMRK IERKHGCAPL PIFALTADMQ
2310 2320 2330 2340
PTMETQFQEV GITHYLSKPF KKETLIKMLL QYLVNGTDGN ANTS
Length:2,344
Mass (Da):266,850
Last modified:November 1, 1998 - v1
Checksum:i8032B9DBD3E1BF5C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA Translation: CAA20836.1
PIRiT41590
RefSeqiNP_588379.1, NM_001023370.2

Genome annotation databases

EnsemblFungiiSPCC74.06.1; SPCC74.06.1:pep; SPCC74.06
GeneIDi2539117
KEGGispo:SPCC74.06

Entry informationi

Entry nameiMAK3_SCHPO
AccessioniPrimary (citable) accession number: O74539
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: November 1, 1998
Last modified: February 28, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome