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Protein

SNF1-like protein kinase ssp2

Gene

ssp2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine protein kinase essential for release from glucose repression via the phosphorylation of scr1 upon glucose deprivation (PubMed:22140232). Catalytic subunit of the AMP-activated protein kinase complex also known as the SNF1 kinase complex (Snf1c), a central regulator of cellular energy homeostasis, which, in response to a fall in intracellular ATP levels, activates energy-producing pathways and inhibits energy-consuming processes (PubMed:22375066). The complex phosphorylates histone H3 to form H3S10ph, which promotes H3K14ac formation, leading to transcriptional activation through TBP recruitment to the promoters (By similarity). Regulates proper cell cycle exit and sexual differentiation (PubMed:22375066). Regulates also ste11 levels under nitrogen deprivation (PubMed:22375066).By similarity2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei63ATPPROSITE-ProRule annotation1
Active sitei156Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi40 – 48ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • polyubiquitin binding Source: PomBase
  • protein serine/threonine kinase activity Source: PomBase

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCarbohydrate metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 5613.
ReactomeiR-SPO-163680. AMPK inhibits chREBP transcriptional activation activity.
R-SPO-200425. Import of palmitoyl-CoA into the mitochondrial matrix.
R-SPO-380972. Energy dependent regulation of mTOR by LKB1-AMPK.

Names & Taxonomyi

Protein namesi
Recommended name:
SNF1-like protein kinase ssp21 Publication (EC:2.7.11.1By similarity)
Gene namesi
Name:ssp21 Publication
ORF Names:SPCC74.03c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC74.03c.
PomBaseiSPCC74.03c. ssp2.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 2 Publications

  • Note: Nuclear translocation occurs under nitrogen and glucose starvation conditions and depends on Thr-189 phosphorylation by ssp1 (PubMed:22375066).1 Publication

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • mitotic spindle pole body Source: PomBase
  • nucleotide-activated protein kinase complex Source: PomBase
  • nucleus Source: PomBase

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Prevents assimilation of glycerol and fails to induce expression of glycerol dehydrogenase gld1 on glycerol medium (PubMed:22140232). Leads to diminished mating efficiency (PubMed:22375066).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi189T → A: Impairs phosphorylation by ssp1 and nuclear localization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000866691 – 576SNF1-like protein kinase ssp2Add BLAST576

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei189Phosphothreonine1 Publication1
Modified residuei442Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylation at Thr-189 by ssp1 is required for nuclear entry in nutritionally stressed cells (PubMed:22375066).1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO74536.
PRIDEiO74536.

PTM databases

iPTMnetiO74536.

Interactioni

Subunit structurei

Component of the AMP-activated protein kinase complex also known as the SNF1 kinase complex (Snf1c), an heterotrimeric complex composed of a catalytic subunit alpha and 2 regulatory subunits beta (amk2) and gamma (cbs2) (PubMed:17289942, PubMed:17937917).2 Publications

GO - Molecular functioni

  • polyubiquitin binding Source: PomBase

Protein-protein interaction databases

BioGridi275681. 222 interactors.
DIPiDIP-29520N.
IntActiO74536. 1 interactor.
MINTiMINT-4679856.
STRINGi4896.SPCC74.03c.1.

Structurei

Secondary structure

1576
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi29 – 31Combined sources3
Beta strandi34 – 43Combined sources10
Beta strandi46 – 53Combined sources8
Turni54 – 56Combined sources3
Beta strandi59 – 66Combined sources8
Helixi67 – 72Combined sources6
Helixi77 – 86Combined sources10
Beta strandi97 – 102Combined sources6
Beta strandi104 – 111Combined sources8
Helixi118 – 125Combined sources8
Helixi130 – 149Combined sources20
Turni159 – 161Combined sources3
Beta strandi162 – 164Combined sources3
Beta strandi170 – 172Combined sources3
Turni178 – 183Combined sources6
Turni194 – 196Combined sources3
Helixi199 – 201Combined sources3
Helixi206 – 208Combined sources3
Helixi210 – 226Combined sources17
Beta strandi230 – 232Combined sources3
Beta strandi234 – 236Combined sources3
Helixi256 – 263Combined sources8
Helixi270 – 272Combined sources3
Helixi276 – 279Combined sources4
Helixi283 – 286Combined sources4
Helixi291 – 293Combined sources3
Helixi306 – 314Combined sources9
Helixi320 – 326Combined sources7
Beta strandi329 – 331Combined sources3
Helixi338 – 351Combined sources14
Beta strandi453 – 460Combined sources8
Helixi462 – 476Combined sources15
Beta strandi486 – 489Combined sources4
Helixi492 – 495Combined sources4
Beta strandi496 – 502Combined sources7
Helixi504 – 508Combined sources5
Beta strandi513 – 525Combined sources13
Beta strandi528 – 541Combined sources14
Turni545 – 547Combined sources3
Helixi562 – 575Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OOXX-ray2.60A/C440-576[»]
2OOYX-ray2.88A/C440-576[»]
2QR1X-ray2.70A/C440-576[»]
2QRCX-ray2.70A/C440-576[»]
2QRDX-ray2.41A/C440-576[»]
2QREX-ray3.01A/C440-576[»]
3H4JX-ray2.80A/B25-351[»]
ProteinModelPortaliO74536.
SMRiO74536.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO74536.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 285Protein kinasePROSITE-ProRule annotation1 PublicationAdd BLAST252
Domaini304 – 345UBAPROSITE-ProRule annotationAdd BLAST42

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni292 – 348Auto-inhibitory domain (AID)1 PublicationAdd BLAST57

Domaini

The regulatory domain (RS) also called auto-inhibitory domain (AID) inhibits kinase activity of the protein kinase domain (KD) (PubMed:19474788).1 Publication
The ubiquitin-associated domain (UBA) localized within the AID dampens kinase activation, probably by restraining alpha-gamma associations (By similarity).By similarity

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000233016.
InParanoidiO74536.
KOiK12761.
OMAiTIDSNIC.
OrthoDBiEOG092C0QJU.
PhylomeDBiO74536.

Family and domain databases

CDDicd14334. UBA_SNF1_fungi. 1 hit.
InterProiView protein in InterPro
IPR032270. AMPK_C.
IPR028375. KA1/Ssp2_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR013896. SNF1_UBA.
IPR015940. UBA.
PfamiView protein in Pfam
PF16579. AdenylateSensor. 1 hit.
PF00069. Pkinase. 1 hit.
PF08587. UBA_2. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF103243. SSF103243. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50030. UBA. 1 hit.

Sequencei

Sequence statusi: Complete.

O74536-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQPQEVDLME NSTMRNGARV LPPEAISKRH IGPYIIRETL GEGSFGKVKL
60 70 80 90 100
ATHYKTQQKV ALKFISRQLL KKSDMHMRVE REISYLKLLR HPHIIKLYDV
110 120 130 140 150
ITTPTDIVMV IEYAGGELFD YIVEKKRMTE DEGRRFFQQI ICAIEYCHRH
160 170 180 190 200
KIVHRDLKPE NLLLDDNLNV KIADFGLSNI MTDGNFLKTS CGSPNYAAPE
210 220 230 240 250
VINGKLYAGP EVDVWSCGIV LYVMLVGRLP FDDEFIPNLF KKVNSCVYVM
260 270 280 290 300
PDFLSPGAQS LIRRMIVADP MQRITIQEIR RDPWFNVNLP DYLRPMEEVQ
310 320 330 340 350
GSYADSRIVS KLGEAMGFSE DYIVEALRSD ENNEVKEAYN LLHENQVIQE
360 370 380 390 400
KSHLSKSKRV DSFLSVSPPA FSEYTSELQK KSKQELIDPT LEGPRWTVSD
410 420 430 440 450
PPTYAKQTID SNICVLVPTA EKNKLEMRTL ADAASAVDTS QSTRKKSRRN
460 470 480 490 500
KWHFGVRCRG DAPEILLAVY RALQRAGAQF TVPKPVNGKY RSDMYTIKSR
510 520 530 540 550
WEIPHCKREG KNTYAYIELQ LYEVMPGCFM LDVKSNGYKD IYSHPERTAD
560 570
HGMDDLKSSF PFLDLCAMLV CKLFSA
Length:576
Mass (Da):65,996
Last modified:November 1, 1998 - v1
Checksum:iE5857E8F171E7B50
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA20833.1.
PIRiT41587.
RefSeqiNP_588376.1. NM_001023367.2.

Genome annotation databases

EnsemblFungiiSPCC74.03c.1; SPCC74.03c.1:pep; SPCC74.03c.
GeneIDi2539109.
KEGGispo:SPCC74.03c.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiSNF1_SCHPO
AccessioniPrimary (citable) accession number: O74536
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1998
Last modified: July 5, 2017
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families