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O74518

- CID12_SCHPO

UniProt

O74518 - CID12_SCHPO

Protein

Poly(A) RNA polymerase cid12

Gene

cid12

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Has a role in the RNA interference (RNAi) pathway which is important for heterochromatin formation and accurate chromosome segregation. A member of the RNA-directed RNA polymerase complex (RDRC) which is involved in the generation of small interfering RNAs (siRNAs) and mediate their association with the RNA-induced transcriptional silencing (RITS) complex. RITS acts as a priming complex for dsRNA synthesis at the site of non-coding centromeric RNA.1 Publication

    Catalytic activityi

    ATP + RNA(n) = diphosphate + RNA(n+1).

    Cofactori

    Magnesium or manganese.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi77 – 771Magnesium or manganese; catalyticBy similarity
    Metal bindingi79 – 791Magnesium or manganese; catalyticBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. polynucleotide adenylyltransferase activity Source: UniProtKB-EC
    4. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. attachment of spindle microtubules to kinetochore involved in mitotic sister chromatid segregation Source: PomBase
    2. chromatin silencing at centromere Source: PomBase
    3. chromatin silencing by small RNA Source: PomBase
    4. mitotic DNA damage checkpoint Source: PomBase
    5. mitotic sister chromatid segregation Source: PomBase
    6. mitotic spindle assembly checkpoint Source: PomBase
    7. RNA polyadenylation Source: PomBase
    8. sister chromatid biorientation Source: PomBase

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Cell cycle, Chromosome partition, RNA-mediated gene silencing

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Poly(A) RNA polymerase cid12 (EC:2.7.7.19)
    Short name:
    PAP
    Alternative name(s):
    Caffeine-induced death protein 12
    Polynucleotide adenylyltransferase cid12
    Gene namesi
    Name:cid12
    ORF Names:SPCC663.12
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome III

    Organism-specific databases

    PomBaseiSPCC663.12.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: PomBase
    2. nuclear RNA-directed RNA polymerase complex Source: PomBase
    3. nucleus Source: PomBase
    4. RNA-directed RNA polymerase complex Source: PomBase

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 336336Poly(A) RNA polymerase cid12PRO_0000256156Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei325 – 3251Phosphoserine1 Publication
    Modified residuei327 – 3271Phosphothreonine1 Publication
    Modified residuei329 – 3291Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiO74518.

    Interactioni

    Subunit structurei

    Cid12, hrr1 and rdp1 interact forming the RNA-directed RNA polymerase complex (RDRC). The RDRC complex interacts with the RITS complex via interaction between ago1 and hrr1. Clr4 has a role in mediating this interaction.

    Protein-protein interaction databases

    BioGridi275988. 121 interactions.
    DIPiDIP-60035N.
    MINTiMINT-4679737.
    STRINGi4896.SPCC663.12-1.

    Structurei

    3D structure databases

    ProteinModelPortaliO74518.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini209 – 26355PAP-associatedAdd
    BLAST

    Sequence similaritiesi

    Belongs to the DNA polymerase type-B-like family.Curated
    Contains 1 PAP-associated domain.Curated

    Phylogenomic databases

    eggNOGiCOG5260.
    KOiK11700.
    OrthoDBiEOG7CNZS6.
    PhylomeDBiO74518.

    Family and domain databases

    InterProiIPR002058. PAP_assoc.
    [Graphical view]
    PfamiPF03828. PAP_assoc. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O74518-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKVLLELHS VPWNEEGLSD NARLYSFLEF VSPKIEELKY RKLLLEKLQT    50
    HIREVVLDAE LQVYGSMYIG TTLSISDVDV SLKSPRVGEL EKRRVTMVLR 100
    KYLDADADFH SSARVPRINL VDVSGIGVDL TFGNDKACRT AELQKAYNEE 150
    HPIFGRLLML LKHWLFERDL ENVHHGGIAS CALSYMLIGW LEMRFHKKGI 200
    DSEVQPIRAL LQKFFYFWGV EWTYELFVLR PLTGQIVPKL QKGWLNEVQP 250
    NLLSIEDPID RNNDIGKQSF QISMIKAAFV ASANELLSDK TWFSTFAITE 300
    DEMFLCKQFE NVINTKRSLV EGYDSDTESD ELQAGG 336
    Length:336
    Mass (Da):38,558
    Last modified:November 1, 1998 - v1
    Checksum:iBF56B701691F8737
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329672 Genomic DNA. Translation: CAA20372.1.
    PIRiT41543.
    RefSeqiNP_588273.1. NM_001023263.2.

    Genome annotation databases

    EnsemblFungiiSPCC663.12.1; SPCC663.12.1:pep; SPCC663.12.
    GeneIDi2539423.
    KEGGispo:SPCC663.12.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329672 Genomic DNA. Translation: CAA20372.1 .
    PIRi T41543.
    RefSeqi NP_588273.1. NM_001023263.2.

    3D structure databases

    ProteinModelPortali O74518.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 275988. 121 interactions.
    DIPi DIP-60035N.
    MINTi MINT-4679737.
    STRINGi 4896.SPCC663.12-1.

    Proteomic databases

    MaxQBi O74518.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPCC663.12.1 ; SPCC663.12.1:pep ; SPCC663.12 .
    GeneIDi 2539423.
    KEGGi spo:SPCC663.12.

    Organism-specific databases

    PomBasei SPCC663.12.

    Phylogenomic databases

    eggNOGi COG5260.
    KOi K11700.
    OrthoDBi EOG7CNZS6.
    PhylomeDBi O74518.

    Miscellaneous databases

    NextBioi 20800587.

    Family and domain databases

    InterProi IPR002058. PAP_assoc.
    [Graphical view ]
    Pfami PF03828. PAP_assoc. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "Two RNAi complexes, RITS and RDRC, physically interact and localize to noncoding centromeric RNAs."
      Motamedi M.R., Verdel A., Colmenares S.U., Gerber S.A., Gygi S.P., Moazed D.
      Cell 119:789-802(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, COMPOSITION OF THE RDRC AND RITS COMPLEXES, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
    3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-325; THR-327 AND SER-329, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiCID12_SCHPO
    AccessioniPrimary (citable) accession number: O74518
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2006
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3