O74515 (ASF1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histone chaperone cia1

Alternative name(s):
Anti-silencing function protein 1

Gene names

Name:	cia1
Synonyms:	asf1
ORF Names:	SPCC663.05c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Ref.1
Subunit structure	Interacts with histone H3 and histone H4 By similarity.
Subcellular location	Nucleus Ref.3.
Sequence similarities	Belongs to the anti-silencing protein family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Coiled coil
Molecular function	Chaperone Chromatin regulator
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA replication-dependent nucleosome assembly Inferred from sequence or structural similarity. Source: GeneDB_Spombe DNA replication-independent nucleosome assembly Inferred from sequence or structural similarity. Source: GeneDB_Spombe chromatin silencing Inferred from sequence or structural similarity. Source: GeneDB_Spombe histone acetylation Inferred from sequence or structural similarity. Source: GeneDB_Spombe histone exchange Inferred from sequence or structural similarity. Source: GeneDB_Spombe nucleosome disassembly Inferred from sequence or structural similarity. Source: GeneDB_Spombe transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromatin assembly complex Inferred from direct assay. Source: GeneDB_Spombe nuclear chromatin Non-traceable author statement. Source: GeneDB_Spombe
Molecular function	histone binding Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 262

262

Histone chaperone cia1

PRO_0000089743

Regions

Coiled coil

173 – 196

24

Potential

Coiled coil

223 – 253

31

Potential

Compositional bias

176 – 259

84

Glu-rich

Secondary structure

1

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262

Helix Strand Turn

Details...

Beta strand

3 – 13

11

Beta strand

15 – 17

3

Beta strand

22 – 30

9

Beta strand

38 – 47

10

Beta strand

54 – 62

9

Beta strand

67 – 76

10

Helix

81 – 83

3

Helix

87 – 90

4

Beta strand

91 – 102

12

Beta strand

105 – 118

14

Helix

123 – 125

3

Helix

128 – 131

4

Helix

138 – 140

3

Beta strand

141 – 145

5

Beta strand

151 – 154

4

Sequences

	Sequence		Length	Mass (Da)
	O74515 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: FA4E491BC8B49171	FASTA	262	29,431
	10 20 30 40 50 60 MSIVNILSVN VLNNPAKFSD PYKFEITFEC LEPLKSDLEW KLTYVGSATS QSYDQILDTL 70 80 90 100 110 120 LVGPIPIGIN KFVFEADPPN IDLLPQLSDV LGVTVILLSC AYEDNEFVRV GYYVNNEMEG 130 140 150 160 170 180 LNLQEMDDAE IKKVKVDISK VWRSILAEKP RVTRFNIQWD NPDFDDAPPV QPDADEEEEE 190 200 210 220 230 240 EEADEMEEEF DEEGEGDEEE EEEDDGDGDG EGDGDGEGEN DGKGSEEEEE EEIDIEEEEE 250 260 ESALANASAA EEKPEEKPET SQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Polyanionic stretch-deleted histone chaperone cia1/Asf1p is functional both in vivo and in vitro." Umehara T., Chimura T., Ichikawa N., Horikoshi M. Genes Cells 7:59-73(2002) [PubMed: 11856374] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[3]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB031397 Genomic DNA. Translation: BAB82475.1.
CU329672 Genomic DNA. Translation: CAA20365.1.

PIR

T41536.

RefSeq

NP_588267.1. NM_001023257.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CU9	X-ray	1.80	A	1-161	[»]
2DZE	X-ray	1.80	A/B	1-161	[»]
2Z34	X-ray	2.40	A/B	1-161	[»]
2Z3F	X-ray	2.70	A/B/C/D/E/F/G/H	1-161	[»]

ProteinModelPortal

O74515.

SMR

O74515. Positions 1-161.

ModBase

Search...

Protein-protein interaction databases

STRING

O74515.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

SPCC663.05c.1; SPCC663.05c.1:pep; SPCC663.05c.

GeneID

2539568.

GenomeReviews

Gene locus cia1 in contig CU329672_GR.

KEGG

spo:SPCC663.05c.

NMPDR

fig|4896.1.peg.605.

Organism-specific databases

GeneDB_Spombe

SPCC663.05c.

Phylogenomic databases

eggNOG

fuNOG05864.

GeneTree

EFGT00050000000028.

HOGENOM

HBG611346.

OMA

ADAKEDI.

OrthoDB

EOG4BP4NB.

Enzyme and pathway databases

BioCyc

SPOM-XXX-01:SPOM-XXX-01-000196-MONOMER.

Gene expression databases

ArrayExpress

O74515.

Family and domain databases

InterPro

IPR017282. Hist_deposition_Asf1.
IPR006818. Histone_chaperone_ASF1-like.
[Graphical view]

Gene3D

G3DSA:2.60.40.1490. Anti-silence. 1 hit.

KO

K10753.

PANTHER

PTHR12040. Anti-silence. 1 hit.

Pfam

PF04729. ASF1_hist_chap. 1 hit.
[Graphical view]

PIRSF

PIRSF037759. Histone_Asf1. 1 hit.

SUPFAM

SSF101546. Anti-silence. 1 hit.

ProtoNet

Search...

Entry information

Entry name

ASF1_SCHPO

Accession

Primary (citable) accession number: O74515

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2002
Last sequence update:	November 1, 1998
Last modified:	December 14, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program