Reviewed, UniProtKB/Swiss-Prot O74515 (ASF1_SCHPO)

Names and origin

Protein names

Recommended name:
    Histone chaperone cia1
Alternative name(s):
    Anti-silencing function protein 1

Gene names

Name:	cia1
Synonyms:	asf1
ORF Names:	SPCC663.05c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Ref.1
Subunit structure	Interacts with histone H3 and histone H4 By similarity.
Subcellular location	Nucleus Ref.3.
Sequence similarities	Belongs to the anti-silencing protein family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Coiled coil
Molecular function	Chaperone Chromatin regulator
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	chromatin assembly or disassembly Inferred from electronic annotation. Source: InterPro chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromatin assembly complex Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	protein binding Inferred from physical interaction. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 262

262

Histone chaperone cia1

PRO_0000089743

Regions

Coiled coil

173 – 196

24

Potential

Coiled coil

223 – 253

31

Potential

Compositional bias

176 – 259

84

Glu-rich

Secondary structure

1

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262

Helix Strand Turn

Details...

Beta strand

3 – 13

11

Beta strand

15 – 17

3

Beta strand

22 – 30

9

Beta strand

38 – 47

10

Beta strand

54 – 62

9

Beta strand

67 – 76

10

Helix

81 – 83

3

Helix

87 – 90

4

Beta strand

91 – 102

12

Beta strand

105 – 118

14

Helix

123 – 125

3

Helix

128 – 131

4

Helix

138 – 140

3

Beta strand

141 – 145

5

Beta strand

151 – 154

4

Sequences

	Sequence		Length	Mass (Da)
	O74515-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: FA4E491BC8B49171	FASTA	262	29,431
	10 20 30 40 50 60 MSIVNILSVN VLNNPAKFSD PYKFEITFEC LEPLKSDLEW KLTYVGSATS QSYDQILDTL 70 80 90 100 110 120 LVGPIPIGIN KFVFEADPPN IDLLPQLSDV LGVTVILLSC AYEDNEFVRV GYYVNNEMEG 130 140 150 160 170 180 LNLQEMDDAE IKKVKVDISK VWRSILAEKP RVTRFNIQWD NPDFDDAPPV QPDADEEEEE 190 200 210 220 230 240 EEADEMEEEF DEEGEGDEEE EEEDDGDGDG EGDGDGEGEN DGKGSEEEEE EEIDIEEEEE 250 260 ESALANASAA EEKPEEKPET SQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Polyanionic stretch-deleted histone chaperone cia1/Asf1p is functional both in vivo and in vitro." Umehara T., Chimura T., Ichikawa N., Horikoshi M. Genes Cells 7:59-73(2002) [PubMed: 11856374] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[3]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB031397 Genomic DNA. Translation: BAB82475.1.
CU329672 Genomic DNA. Translation: CAA20365.1.

PIR

T41536.

RefSeq

NP_588267.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CU9	X-ray	1.80	A	1-161	[»]
2DZE	X-ray	1.80	A/B	1-161	[»]
2Z34	X-ray	2.40	A/B	1-161	[»]
2Z3F	X-ray	2.70	A/B/C/D/E/F/G/H	1-161	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

O74515.

Genome annotation databases

GeneID

2539568.

GenomeReviews

Gene locus cia1 in contig CU329672_GR.

KEGG

spo:SPCC663.05c.

NMPDR

fig|4896.1.peg.605.

Organism-specific databases

GeneDB_Spombe

SPCC663.05c.

Phylogenomic databases

eggNOG

fuNOG05864.

HOGENOM

HBG611346.

OMA

VEENGSK.

OrthoDB

EOG9ZCVNB.

PhylomeDB

O74515.

Gene expression databases

ArrayExpress

O74515.

Family and domain databases

InterPro

IPR017282. Hist_deposition_Asf1.
IPR006818. Histone_chaperone_ASF1-like.
[Graphical view]

Gene3D

G3DSA:2.60.40.1490. Anti-silence. 1 hit.

PANTHER

PTHR12040. Anti-silence. 1 hit.

Pfam

PF04729. ASF1_hist_chap. 1 hit.
[Graphical view]

PIRSF

PIRSF037759. Histone_Asf1. 1 hit.

ProtoNet

Search...

Entry information

Entry name

ASF1_SCHPO

Accession

Primary (citable) accession number: O74515

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2002
Last sequence update:	November 1, 1998
Last modified:	January 19, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)