Reviewed, UniProtKB/Swiss-Prot O74484 (MPG1_SCHPO)

Names and origin

Protein names

Recommended name:
    Mannose-1-phosphate guanyltransferase
    EC=2.7.7.13
Alternative name(s):
    GTP-mannose-1-phosphate guanylyltransferase
    GDP-mannose pyrophosphorylase

Gene names

Name:	mpg1
ORF Names:	SPCC1906.01

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in cell wall synthesis where it is required for glycosylation. Involved in cell cycle progression through cell-size checkpoint. Required for the correct assembly of the septum. Ref.1
Catalytic activity	GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose.
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1.
Subcellular location	Cytoplasm. Ref.1
Sequence similarities	Belongs to the transferase hexapeptide repeat family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Septation
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GDP-mannose biosynthetic process Inferred by curator. Source: GeneDB_SPombe barrier septum formation Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell size control checkpoint Ref.1 Inferred from genetic interaction. Source: GeneDB_SPombe cell wall mannoprotein biosynthetic process Inferred by curator. Source: GeneDB_SPombe fungal-type cell wall biogenesis Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe protein amino acid glycosylation Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe
Cellular component	cell tip Ref.1 Inferred from direct assay. Source: GeneDB_SPombe cytosol Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mannose-1-phosphate guanylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Mannose-1-phosphate guanyltransferase		PRO_0000068740
Experimental info
	Sequence conflict	175 – 176	2	GI → VL in BAA13790. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O74484-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 55F55FF0ED017C02	FASTA	363	39,719
	10 20 30 40 50 60 MKALILVGGF GTRLRPLTLT LPKPLVEFGN KPMILHQVEA LAAAGVTDIV LAVNYRPEIM 70 80 90 100 110 120 VEALKKYEKE YNVNITFSVE NEPLGTAGPL ALARDILAKD HSPFFVLNSD VICEYPFADL 130 140 150 160 170 180 AAFHKAHGAE GTIVVTKVEE PSKYGVVVHY PNSESLIERF VEKPVEFVSN RINGGIYILN 190 200 210 220 230 240 PSVLDRIEPR PTSIEKEVFP AMVNDKQLHS FDLEGYWMDV GQPKDYLTGT CLYLSSLRKH 250 260 270 280 290 300 KPEILAPASS NIIGNVLIDP SATIGKNCKI GPNVVIGPNV TIGDGVRLQR CAILKSSRVR 310 320 330 340 350 360 DHAWVKSSIV GWNSTLGSWS RLENVSVLGD DVVVNDEIYV NGGSILPHKS ISANIEVPGT IVM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mpg1, a fission yeast protein required for proper septum structure, is involved in cell cycle progression through cell-size checkpoint." Donoso I., Munoz-Centeno M.C., Sanchez-Duran M.A., Flores A., Daga R.R., Guevara C.M., Bejarano E.R. Mol. Genet. Genomics 274:155-167(2005) [PubMed: 16049679] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION. Strain: ATCC 38366 / 972.
[2]	"Identification of open reading frames in Schizosaccharomyces pombe cDNAs." Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H. DNA Res. 4:363-369(1997) [PubMed: 9501991] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: PR745.
[3]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D89128 mRNA. Translation: BAA13790.1. CU329672 Genomic DNA. Translation: CAA20770.1.
PIR	T41209. T42371.
RefSeq	NP_588405.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O74484.
Genome annotation databases
GeneID	2538743.
GenomeReviews	Gene locus mpg1 in contig CU329672_GR.
KEGG	spo:SPCC1906.01.
NMPDR	fig|4896.1.peg.743.
Organism-specific databases
GeneDB_Spombe	SPCC1906.01.
Phylogenomic databases
OMA	RRIQLQP.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-000318-MON.
BRENDA	2.7.7.13. 653.
Gene expression databases
ArrayExpress	O74484.
Family and domain databases
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR005835. NTP_transferase. [Graphical view]
Pfam	PF00132. Hexapep. 4 hits. PF00483. NTP_transferase. 1 hit. [Graphical view]
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPG1_SCHPO

Accession

Primary (citable) accession number: O74484
Secondary accession number(s): P78779

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	November 1, 1998
Last modified:	November 3, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)