ID   SUMT_SCHPO              Reviewed;         496 AA.
AC   O74468; P78775; Q9URL1;
DT   20-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   27-MAR-2024, entry version 144.
DE   RecName: Full=Probable uroporphyrinogen-III C-methyltransferase;
DE            EC=2.1.1.107;
GN   ORFNames=SPCC1739.06c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 246-496.
RC   STRAIN=PR745;
RX   PubMed=9501991; DOI=10.1093/dnares/4.6.363;
RA   Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.;
RT   "Identification of open reading frames in Schizosaccharomyces pombe
RT   cDNAs.";
RL   DNA Res. 4:363-369(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 407-496.
RA   Kawamukai M.;
RT   "S.pombe MET1 homolog.";
RL   Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Siroheme synthase involved in methionine biosynthesis.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 S-adenosyl-L-methionine + uroporphyrinogen III = H(+) +
CC         precorrin-2 + 2 S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:32459,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57308, ChEBI:CHEBI:57856,
CC         ChEBI:CHEBI:58827, ChEBI:CHEBI:59789; EC=2.1.1.107;
CC   -!- SIMILARITY: Belongs to the precorrin methyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; CU329672; CAA20780.1; -; Genomic_DNA.
DR   EMBL; D89123; BAA13785.1; -; mRNA.
DR   EMBL; AB009602; BAA23999.1; -; mRNA.
DR   PIR; T41114; T41114.
DR   PIR; T42365; T42365.
DR   PIR; T43302; T43302.
DR   RefSeq; NP_588414.1; NM_001023405.2.
DR   AlphaFoldDB; O74468; -.
DR   SMR; O74468; -.
DR   BioGRID; 275519; 27.
DR   STRING; 284812.O74468; -.
DR   iPTMnet; O74468; -.
DR   MaxQB; O74468; -.
DR   PaxDb; 4896-SPCC1739-06c-1; -.
DR   EnsemblFungi; SPCC1739.06c.1; SPCC1739.06c.1:pep; SPCC1739.06c.
DR   GeneID; 2538945; -.
DR   KEGG; spo:SPCC1739.06c; -.
DR   PomBase; SPCC1739.06c; -.
DR   VEuPathDB; FungiDB:SPCC1739.06c; -.
DR   eggNOG; KOG1527; Eukaryota.
DR   HOGENOM; CLU_011276_11_1_1; -.
DR   InParanoid; O74468; -.
DR   OMA; EDLNWSA; -.
DR   PhylomeDB; O74468; -.
DR   PRO; PR:O74468; -.
DR   Proteomes; UP000002485; Chromosome III.
DR   GO; GO:0004851; F:uroporphyrin-III C-methyltransferase activity; IBA:GO_Central.
DR   GO; GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0019354; P:siroheme biosynthetic process; IBA:GO_Central.
DR   GO; GO:0000103; P:sulfate assimilation; IEA:InterPro.
DR   CDD; cd11642; SUMT; 1.
DR   Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR   InterPro; IPR000878; 4pyrrol_Mease.
DR   InterPro; IPR035996; 4pyrrol_Methylase_sf.
DR   InterPro; IPR014777; 4pyrrole_Mease_sub1.
DR   InterPro; IPR014776; 4pyrrole_Mease_sub2.
DR   InterPro; IPR006366; CobA/CysG_C.
DR   InterPro; IPR012066; Met1_fungi.
DR   InterPro; IPR003043; Uropor_MeTrfase_CS.
DR   NCBIfam; TIGR01469; cobA_cysG_Cterm; 1.
DR   PANTHER; PTHR45790; SIROHEME SYNTHASE-RELATED; 1.
DR   PANTHER; PTHR45790:SF6; UROPORPHYRINOGEN-III C-METHYLTRANSFERASE; 1.
DR   Pfam; PF13241; NAD_binding_7; 1.
DR   Pfam; PF00590; TP_methylase; 1.
DR   PIRSF; PIRSF036555; SUMT_yeast; 1.
DR   SUPFAM; SSF75615; Siroheme synthase middle domains-like; 1.
DR   SUPFAM; SSF53790; Tetrapyrrole methylase; 1.
DR   PROSITE; PS00840; SUMT_2; 1.
PE   2: Evidence at transcript level;
KW   Amino-acid biosynthesis; Methionine biosynthesis; Methyltransferase;
KW   Porphyrin biosynthesis; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..496
FT                   /note="Probable uroporphyrinogen-III C-methyltransferase"
FT                   /id="PRO_0000150386"
FT   CONFLICT        330
FT                   /note="F -> L (in Ref. 2; BAA13785)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        343
FT                   /note="K -> E (in Ref. 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        345
FT                   /note="G -> S (in Ref. 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        379
FT                   /note="A -> P (in Ref. 2; BAA13785)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   496 AA;  54712 MW;  296D3CD7B9C1536E CRC64;
     MIVSWSLKDA VVVVVGSGKN AYRRVNLCLS ENSSKIFWFC RSSADGGFDV GHLQLDAKKS
     DAIEILPISE FDPVRSLTTL GKEETDFLVD AVFVSESNHH EKEILHRTCK RYRIPLNIID
     NPSLCSFTLP ATWSEPPLQI SLSTSSNGCR LAQRLLRHVV SSLPSGMPEA IERFGRVRAI
     TKTPEKKQWI NHVSDFWPLE KLVRMTEDDL LAIVSDNFSL PLSSSQSSSL ANSYESLSTT
     LDKPSLTLDP EAFPTHKRGS IALIGSGPGS PDLLTVAARK AIMKADYVLA DKLVPEAVLQ
     LIPRHTPLFI ARKFPGNADK AQDELHQVAF DALSRGDYVV RLKQGDPYIY GRGGEEYLFF
     TQHGYVPTVI PGISSALMAP ISAGIPVTHR GVADQFLVCT GTGQKGSMPK IPSFVPTQTT
     VFLMALHRLE ILVQALIESG WPRVLPVCIA ERVSCPDQRF IFSTLEDVVE EYNKYESLPP
     GLLITGYSCN TLRNTA
//