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Protein

Probable ubiquitin carboxyl-terminal hydrolase 16

Gene

ubp16

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei134NucleophilePROSITE-ProRule annotation1
Active sitei388Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

  • thiol-dependent ubiquitin-specific protease activity Source: PomBase

GO - Biological processi

  • protein deubiquitination Source: PomBase
  • ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-SPO-5689880. Ub-specific processing proteases.

Protein family/group databases

MEROPSiC19.A66.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ubiquitin carboxyl-terminal hydrolase 16 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 16
Ubiquitin thioesterase 16
Ubiquitin-specific-processing protease 16
Gene namesi
Name:ubp16
ORF Names:SPCC1682.12c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC1682.12c.
PomBaseiSPCC1682.12c. ubp16.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002483991 – 457Probable ubiquitin carboxyl-terminal hydrolase 16Add BLAST457

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei61Phosphoserine1 Publication1
Modified residuei64Phosphothreonine1 Publication1
Modified residuei65Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO74442.
PRIDEiO74442.

PTM databases

iPTMnetiO74442.

Interactioni

Protein-protein interaction databases

BioGridi275521. 25 interactors.
MINTiMINT-4679081.

Structurei

3D structure databases

ProteinModelPortaliO74442.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini125 – 429USPAdd BLAST305

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

InParanoidiO74442.
KOiK11855.
OMAiIVCALEM.
OrthoDBiEOG092C5R71.
PhylomeDBiO74442.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O74442-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLATLQGQS LDFAVKHSLD DLLKNPVRFR PAVVSSPSVP EGTYTVLNNP
60 70 80 90 100
KQSTVSRKSF SAPTSPTRNK RSGSSSQEYK KSSGTRGDGA NDFVDEDPAF
110 120 130 140 150
IPPARILFPE EKLSMEWDNI MPNAPGLVNL GNTCFMNSVL QLMTQTPPLV
160 170 180 190 200
QYLLSGQHSL SCRMNACVLC RMEQHVARAY PNKGTKRASA FKPSGIQSML
210 220 230 240 250
KVISSHFRPY RQEDAHEFMR YLVDAWQKSC LQNHKNLDHP SRETSVVHRI
260 270 280 290 300
FGGYLRQQIL CSVCKKPSNT YQALLDLSVD AKGSSLADSL KHFVHAEKLT
310 320 330 340 350
KQNKYRCENC KQLVDASKQM TIYRAPNILT IHFKRFTFNG FQSSKISKQI
360 370 380 390 400
SYPESFNLGP YMSDPNCSCW YELIGVLVHA GGSTRSGHYY SFCKSSNGVW
410 420 430 440 450
LKFDDDFVSN SSIDRVLNQQ AYILQYKRKS TSSSKHKLNT ENTVTKTSNK

KRRKISF
Length:457
Mass (Da):51,582
Last modified:November 1, 1998 - v1
Checksum:i56AADB2AA996EFF3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA20678.1.
PIRiT41069.
RefSeqiNP_587805.1. NM_001022798.2.

Genome annotation databases

EnsemblFungiiSPCC1682.12c.1; SPCC1682.12c.1:pep; SPCC1682.12c.
GeneIDi2538947.
KEGGispo:SPCC1682.12c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329672 Genomic DNA. Translation: CAA20678.1.
PIRiT41069.
RefSeqiNP_587805.1. NM_001022798.2.

3D structure databases

ProteinModelPortaliO74442.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275521. 25 interactors.
MINTiMINT-4679081.

Protein family/group databases

MEROPSiC19.A66.

PTM databases

iPTMnetiO74442.

Proteomic databases

MaxQBiO74442.
PRIDEiO74442.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC1682.12c.1; SPCC1682.12c.1:pep; SPCC1682.12c.
GeneIDi2538947.
KEGGispo:SPCC1682.12c.

Organism-specific databases

EuPathDBiFungiDB:SPCC1682.12c.
PomBaseiSPCC1682.12c. ubp16.

Phylogenomic databases

InParanoidiO74442.
KOiK11855.
OMAiIVCALEM.
OrthoDBiEOG092C5R71.
PhylomeDBiO74442.

Enzyme and pathway databases

ReactomeiR-SPO-5689880. Ub-specific processing proteases.

Miscellaneous databases

PROiO74442.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBP16_SCHPO
AccessioniPrimary (citable) accession number: O74442
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: November 1, 1998
Last modified: October 5, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.